primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score matK_p1 matK 1 ACTGCATGAACATTTCAGAATCG AGTTTACCTGTCTCCGAGGT 23 20 39.1 50.0 58.0 57.7 15.6 0.0 3.327671 2038 3552 1722-1722 1.000000 53.6 matK_p2 matK 2 CTGCATGAACATTTCAGAATCGA AGTTTACCTGTCTCCGAGGT 23 20 39.1 50.0 57.8 57.7 0.0 0.0 3.531757 2038 3552 1721-1721 1.000000 51.9 matK_p3 matK 3 TCAACCTTTACTGCATGAACA AGTTTACCTGTCTCCGAGGT 21 20 38.1 50.0 55.7 57.7 0.0 0.0 3.587233 2038 3552 1731-1731 1.000000 51.5 matK_p4 matK 4 TGCATGAACATTTCAGAATCGA AGTTTACCTGTCTCCGAGGT 22 20 36.4 50.0 56.7 57.7 0.0 0.0 3.601165 2038 3552 1720-1720 1.000000 51.4 matK_p5 matK 5 CAGTTGATTCAACCTTTACTGCA AGTTTACCTGTCTCCGAGGT 23 20 39.1 50.0 57.6 57.7 22.7 0.0 3.743147 2038 3552 1739-1739 1.000000 50.2 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 AAAGCCGAGTACTCTACCGT AGAAGGCGGAGGTTTTTACG 20 20 50.0 50.0 58.2 58.2 20.3 0.0 0.363819 4281 5133 940-943 1.000000 77.4 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 AAGCCGAGTACTCTACCGTT AGAAGGCGGAGGTTTTTACG 20 20 50.0 50.0 58.2 58.2 20.3 0.0 0.363819 4281 5133 939-942 1.000000 77.4 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 TCCTAGCCGCACTTAAAAGC AGAAGGCGGAGGTTTTTACG 20 20 50.0 50.0 58.3 58.2 0.0 0.0 0.461237 4281 5133 955-958 1.000000 76.6 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CCGCACTTAAAAGCCGAGTA AGAAGGCGGAGGTTTTTACG 20 20 50.0 50.0 58.3 58.2 5.3 0.0 0.478205 4281 5133 949-952 1.000000 76.5 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 AAGCCGAGTACTCTACCGTT GGTTTTTACGGAACTTCGCC 20 20 50.0 50.0 58.2 57.7 20.3 0.0 0.489225 4281 5133 929-932 1.000000 76.4 trnS-GCU-trnG_p1 trnS-GCU-trnG 1 CAATCCGACGCTTTAGTCCA GAATTAAACCGAGGGACCCC 20 20 50.0 55.0 58.0 58.0 0.0 7.0 0.053027 9261 10100 953-956 1.000000 80.7 trnS-GCU-trnG_p2 trnS-GCU-trnG 2 AATCCGACGCTTTAGTCCAC GAATTAAACCGAGGGACCCC 20 20 50.0 55.0 58.0 58.0 0.0 7.0 0.054415 9261 10100 952-955 1.000000 80.7 trnS-GCU-trnG_p3 trnS-GCU-trnG 3 ACGGAAAGAGAGGGATTCGA GAATTAAACCGAGGGACCCC 20 20 50.0 55.0 58.2 58.0 0.0 7.0 0.199792 9261 10100 1007-1010 1.000000 79.5 trnS-GCU-trnG_p4 trnS-GCU-trnG 4 TGGAAACGGAAAGAGAGGGA GAATTAAACCGAGGGACCCC 20 20 50.0 55.0 58.3 58.0 0.0 7.0 0.323899 9261 10100 1012-1015 1.000000 78.5 trnS-GCU-trnG_p5 trnS-GCU-trnG 5 AGCAATCCGACGCTTTAGTC GAATTAAACCGAGGGACCCC 20 20 50.0 55.0 58.4 58.0 0.0 7.0 0.406867 9261 10100 955-958 1.000000 77.9 atpF-atpH_p1 atpF-atpH 1 CAGTGACCCAAGGAAACGAA CGGAGGGAAAAATACGAGGT 20 20 50.0 50.0 58.0 57.3 0.0 0.0 0.724056 14120 14569 604-617 1.000000 74.9 atpF-atpH_p2 atpF-atpH 2 CAGTGACCCAAGGAAACGAA CGAGGCGGAGGGAAAAATAC 20 20 50.0 55.0 58.0 58.7 0.0 0.0 0.741479 14120 14569 609-622 1.000000 74.7 atpF-atpH_p3 atpF-atpH 3 CAGTGACCCAAGGAAACGAA GAGGCGGAGGGAAAAATACG 20 20 50.0 55.0 58.0 58.7 0.0 0.0 0.741479 14120 14569 608-621 1.000000 74.7 atpF-atpH_p4 atpF-atpH 4 CAGTGACCCAAGGAAACGAA GCGGAGGGAAAAATACGAGG 20 20 50.0 55.0 58.0 58.7 0.0 0.0 0.741479 14120 14569 605-618 1.000000 74.7 atpF-atpH_p5 atpF-atpH 5 CAGTGACCCAAGGAAACGAA GGCGGAGGGAAAAATACGAG 20 20 50.0 55.0 58.0 58.7 0.0 0.0 0.741479 14120 14569 606-619 1.000000 74.7 rps2-rpoC2_p1 rps2-rpoC2 1 ACCAAAATGAACCCCTGCTT TTCGAGGGGAAAATGAGAGA 20 20 45.0 45.0 57.9 55.9 0.0 0.0 2.239295 17604 17844 421-421 1.000000 63.4 rps2-rpoC2_p2 rps2-rpoC2 2 AATGAACCCCTGCTTCCATC TTCGAGGGGAAAATGAGAGA 20 20 50.0 45.0 57.9 55.9 0.0 0.0 2.287628 17604 17844 416-416 1.000000 63.0 rps2-rpoC2_p3 rps2-rpoC2 3 GAACCCCTGCTTCCATCATC TTCGAGGGGAAAATGAGAGA 20 20 55.0 45.0 58.3 55.9 0.0 0.0 2.452507 17604 17844 413-413 1.000000 61.7 rps2-rpoC2_p4 rps2-rpoC2 4 ACCAAAATGAACCCCTGCTT TCGAGGGGAAAATGAGAGAT 20 20 45.0 45.0 57.9 55.6 0.0 0.0 2.476792 17604 17844 420-420 1.000000 61.5 rps2-rpoC2_p5 rps2-rpoC2 5 GACCAAAATGAACCCCTGCT TTCGAGGGGAAAATGAGAGA 20 20 50.0 45.0 58.4 55.9 0.0 0.0 2.505067 17604 17844 422-422 1.000000 61.3 rpoC2_p1 rpoC2 1 AAAGCAGGATGTCCATGACG CCTGGTCGAATTGGGAGAAG 20 20 50.0 55.0 58.3 58.0 1.3 0.0 0.287077 20891 21010 207-207 1.000000 78.5 rpoC2_p2 rpoC2 2 AAAGCAGGATGTCCATGACG GTCGAATTGGGAGAAGCTGT 20 20 50.0 50.0 58.3 57.9 1.3 0.0 0.357843 20891 21010 203-203 1.000000 77.9 rpoC2_p3 rpoC2 3 GGATGTCCATGACGTGTACG CCTGGTCGAATTGGGAGAAG 20 20 55.0 55.0 58.4 58.0 0.0 0.0 0.473275 20891 21010 201-201 1.000000 77.0 rpoC2_p4 rpoC2 4 AAAGCAGGATGTCCATGACG TGGTCGGAGTCCTACTCATG 20 20 50.0 55.0 58.3 58.2 1.3 13.2 0.493271 20891 21010 231-231 1.000000 76.7 rpoC2_p5 rpoC2 5 GGATGTCCATGACGTGTACG GTCGAATTGGGAGAAGCTGT 20 20 55.0 50.0 58.4 57.9 0.0 0.0 0.544041 20891 21010 197-197 1.000000 76.5 rpoC1_p1 rpoC1 1 GAGAGGACCGAATTCACACC TTGCTCGTTCGAGAACTACG 20 20 55.0 50.0 58.0 58.0 6.2 23.5 0.051793 22173 24960 2962-2963 1.000000 79.7 rpoC1_p2 rpoC1 2 GGAGAGGACCGAATTCACAC TTGCTCGTTCGAGAACTACG 20 20 55.0 50.0 58.0 58.0 6.2 23.5 0.051793 22173 24960 2963-2964 1.000000 79.7 rpoC1_p3 rpoC1 3 CCTAGTTGAACCGGAGAGGA TTGCTCGTTCGAGAACTACG 20 20 55.0 50.0 58.2 58.0 9.5 23.5 0.191601 22173 24960 2975-2976 1.000000 78.6 rpoC1_p4 rpoC1 4 GGACCGAATTCACACCGTAT TTGCTCGTTCGAGAACTACG 20 20 50.0 50.0 57.7 58.0 6.2 23.5 0.345004 22173 24960 2958-2959 1.000000 77.4 rpoC1_p5 rpoC1 5 GAGAGGACCGAATTCACACC GCTCGTTCGAGAACTACGAT 20 20 55.0 50.0 58.0 57.5 6.2 23.5 0.466835 22173 24960 2960-2961 1.000000 76.4 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CGTCAAGCCCTGATCAATGA CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.110548 28200 29345 1303-1304 1.000000 79.7 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 CGTCAAGCCCTGATCAATGA AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 58.0 58.4 0.0 14.6 0.383911 28200 29345 1285-1286 1.000000 77.5 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 AAAGTTCTTCCGTCAAGCCC CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.4 58.1 0.0 0.0 0.476375 28200 29345 1313-1314 1.000000 76.7 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 ATTCCCTCATTTCCATCCCG CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 57.3 58.1 0.0 0.0 0.746530 28200 29345 1233-1234 1.000000 74.6 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 AAAGTTCTTCCGTCAAGCCC AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 58.4 58.4 0.0 14.6 0.749738 28200 29345 1295-1296 1.000000 74.6 petN-psbM_p1 petN-psbM 1 ACATTTTCCCTTTCGCTCGT TGCTACTGCACTGTTCATTCT 20 21 45.0 42.9 58.1 57.6 0.0 0.0 1.515214 30354 31535 1304-1306 1.000000 68.2 petN-psbM_p2 petN-psbM 2 CCCTTTCGCTCGTAGTATGG TGCTACTGCACTGTTCATTCT 20 21 55.0 42.9 57.9 57.6 0.0 0.0 1.543088 30354 31535 1297-1299 1.000000 68.0 petN-psbM_p3 petN-psbM 3 CCTTTCGCTCGTAGTATGGG TGCTACTGCACTGTTCATTCT 20 21 55.0 42.9 57.9 57.6 0.0 0.0 1.543088 30354 31535 1296-1298 1.000000 68.0 petN-psbM_p4 petN-psbM 4 CTTTCGCTCGTAGTATGGGG TGCTACTGCACTGTTCATTCT 20 21 55.0 42.9 57.9 57.6 0.0 0.0 1.543088 30354 31535 1295-1297 1.000000 68.0 petN-psbM_p5 petN-psbM 5 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 20 21 50.0 42.9 58.2 57.6 0.0 0.0 1.558772 30354 31535 1342-1344 1.000000 67.8 ndhK-ndhC_p1 ndhK-ndhC 1 AGAGAAGCGGCCATAAACTG TTTATGCATGGCGAAAGGGA 20 20 50.0 45.0 58.0 57.9 2.2 10.2 0.166137 53214 53272 212-212 1.000000 79.0 ndhK-ndhC_p2 ndhK-ndhC 2 AGAGAAGCGGCCATAAACTG GGTTCAGTTTATGCATGGCG 20 20 50.0 50.0 58.0 57.8 2.2 10.2 0.223144 53214 53272 219-219 1.000000 78.6 ndhK-ndhC_p3 ndhK-ndhC 3 AGAGAAGCGGCCATAAACTG GTTTATGCATGGCGAAAGGG 20 20 50.0 50.0 58.0 57.8 2.2 10.2 0.238164 53214 53272 213-213 1.000000 78.5 ndhK-ndhC_p4 ndhK-ndhC 4 AGAGAAGCGGCCATAAACTG TGGGCAATGAGTTTCGATGT 20 20 50.0 45.0 58.0 57.8 2.2 0.0 0.228665 53214 53272 294-294 1.000000 78.4 ndhK-ndhC_p5 ndhK-ndhC 5 AGAGAAGCGGCCATAAACTG TTATGCATGGCGAAAGGGAG 20 20 50.0 50.0 58.0 58.3 2.2 10.2 0.350699 53214 53272 211-211 1.000000 77.6 atpB-rbcL_p1 atpB-rbcL 1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 20 21 55.0 47.6 58.0 58.0 9.5 0.0 1.063706 57785 58525 878-887 1.000000 72.6 atpB-rbcL_p2 atpB-rbcL 2 GGGGGAAAAGCTACATCCAG CACCAGCTTTGAATCCAACAC 20 21 55.0 47.6 57.9 58.0 12.8 0.0 1.075858 57785 58525 891-900 1.000000 72.5 atpB-rbcL_p3 atpB-rbcL 3 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 20 21 55.0 42.9 58.0 58.1 9.5 0.0 1.096994 57785 58525 877-886 1.000000 72.3 atpB-rbcL_p4 atpB-rbcL 4 GGGGGAAAAGCTACATCCAG ACCAGCTTTGAATCCAACACT 20 21 55.0 42.9 57.9 58.1 12.8 0.0 1.109145 57785 58525 890-899 1.000000 72.2 atpB-rbcL_p5 atpB-rbcL 5 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 20 21 50.0 47.6 58.1 58.0 9.5 0.0 1.143136 57785 58525 876-885 1.000000 71.9 accD-psaI_p1 accD-psaI 1 TGGGATCTGTAGTAGGCGAG GGCACCAAAACAGAGGGTAA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.027633 62103 62796 1198-1199 1.000000 80.6 accD-psaI_p2 accD-psaI 2 AAGGTTCACAAGCAGCTGAA GGCACCAAAACAGAGGGTAA 20 20 45.0 50.0 57.9 58.0 20.4 0.0 0.067431 62103 62796 868-869 1.000000 80.6 accD-psaI_p3 accD-psaI 3 GCGAGAAAATCACCCGTTTG GGCACCAAAACAGAGGGTAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.046547 62103 62796 1183-1184 1.000000 80.5 accD-psaI_p4 accD-psaI 4 TGGGATCTGTAGTAGGCGAG TCCGCATGCATAAAAGCTCT 20 20 55.0 45.0 58.0 58.0 0.0 19.6 0.060026 62103 62796 1406-1406 1.000000 80.3 accD-psaI_p5 accD-psaI 5 TGGGATCTGTAGTAGGCGAG TTGTTTTTCGAGACCCCGTT 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.058591 62103 62796 1746-1746 1.000000 80.1 psaJ-rpl33_p1 psaJ-rpl33 1 ACCCGTGACATTTTGTACCC CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.202195 70739 71167 1246-1250 1.000000 78.7 psaJ-rpl33_p2 psaJ-rpl33 2 CCCGTGACATTTTGTACCCA CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.203538 70739 71167 1245-1249 1.000000 78.7 psaJ-rpl33_p3 psaJ-rpl33 3 ACAGGATTTGAACCCGTGAC CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.210231 70739 71167 1257-1261 1.000000 78.6 psaJ-rpl33_p4 psaJ-rpl33 4 CAGGATTTGAACCCGTGACA CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.211564 70739 71167 1256-1260 1.000000 78.6 psaJ-rpl33_p5 psaJ-rpl33 5 TGACAGGATTTGAACCCGTG CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.211564 70739 71167 1259-1263 1.000000 78.6 petB_p1 petB 1 AGAACCACGAAGTCCTAGCT ATTGTTCCTCCCCCAAATGA 20 20 50.0 45.0 58.1 56.7 0.0 0.0 1.393567 78485 79916 1597-1597 1.000000 69.1 petB_p2 petB 2 AGAACCACGAAGTCCTAGCT TTGTTCCTCCCCCAAATGAT 20 20 50.0 45.0 58.1 56.7 0.0 0.0 1.393567 78485 79916 1596-1596 1.000000 69.1 petB_p3 petB 3 TCACAAGAACCACGAAGTCC ATTGTTCCTCCCCCAAATGA 20 20 50.0 45.0 57.8 56.7 0.0 0.0 1.553975 78485 79916 1602-1602 1.000000 67.8 petB_p4 petB 4 TCACAAGAACCACGAAGTCC TTGTTCCTCCCCCAAATGAT 20 20 50.0 45.0 57.8 56.7 0.0 0.0 1.553975 78485 79916 1601-1601 1.000000 67.8 petB_p5 petB 5 CACAAGAACCACGAAGTCCT TTGTTCCTCCCCCAAATGAT 20 20 50.0 45.0 57.8 56.7 0.0 0.0 1.560169 78485 79916 1600-1600 1.000000 67.7 petD-rpoA_p1 petD-rpoA 1 CATTTCGTCGTCCAGTAGCA TTGGGCATTCTAGAAAAGCA 20 20 50.0 40.0 58.0 55.2 0.0 25.4 2.785724 81283 81470 351-358 1.000000 59.7 petD-rpoA_p2 petD-rpoA 2 GGTTGGGTATTGGAGCAACA TTGGGCATTCTAGAAAAGCA 20 20 50.0 40.0 58.1 55.2 0.0 25.4 2.858651 81283 81470 294-301 1.000000 59.6 petD-rpoA_p3 petD-rpoA 3 TGGTTGGGTATTGGAGCAAC TTGGGCATTCTAGAAAAGCA 20 20 50.0 40.0 58.1 55.2 0.0 25.4 2.858651 81283 81470 295-302 1.000000 59.5 petD-rpoA_p4 petD-rpoA 4 CCAGTAGCAACAACCGTCTT TTGGGCATTCTAGAAAAGCA 20 20 50.0 40.0 58.1 55.2 0.0 25.4 2.897570 81283 81470 340-347 1.000000 58.9 petD-rpoA_p5 petD-rpoA 5 TTTCGTCGTCCAGTAGCAAC TTGGGCATTCTAGAAAAGCA 20 20 50.0 40.0 58.2 55.2 0.0 25.4 3.001885 81283 81470 349-356 1.000000 58.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TTTGTGCCGACAAATACAAA TCCTAAGAGCAGCGTGTCTA 20 20 35.0 50.0 54.3 57.9 0.0 0.0 3.795610 117537 118595 1122-1125 1.000000 50.8 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TTTGTGCCGACAAATACAAA AGCAGCGTGTCTACCAATTT 20 20 35.0 45.0 54.3 57.8 0.0 0.0 3.856336 117537 118595 1115-1118 1.000000 50.4 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTTGTGCCGACAAATACAAA CCTAAGAGCAGCGTGTCTAC 20 20 35.0 55.0 54.3 57.8 0.0 0.0 3.867627 117537 118595 1121-1124 1.000000 50.3 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTTGTGCCGACAAATACAAA CTAAGAGCAGCGTGTCTACC 20 20 35.0 55.0 54.3 57.8 0.0 0.0 3.867627 117537 118595 1120-1123 1.000000 50.3 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TTTGTGCCGACAAATACAAA GAGCAGCGTGTCTACCAATT 20 20 35.0 50.0 54.3 58.3 0.0 0.0 3.944479 117537 118595 1116-1119 1.000000 49.7 ndhD-psaC_p1 ndhD-psaC 1 AAAATTAAGGAACCCGCGGA AGAGGACTGTGTCGGTTGTA 20 20 45.0 50.0 57.8 58.0 30.7 0.0 0.212349 121547 121656 305-306 1.000000 78.8 ndhD-psaC_p2 ndhD-psaC 2 AAATTAAGGAACCCGCGGAT AGAGGACTGTGTCGGTTGTA 20 20 45.0 50.0 57.6 58.0 30.7 0.0 0.439127 121547 121656 304-305 1.000000 77.0 ndhD-psaC_p3 ndhD-psaC 3 CGCGGATATCGGCAAAACTA AGAGGACTGTGTCGGTTGTA 20 20 50.0 50.0 58.5 58.0 28.3 0.0 0.515107 121547 121656 291-292 1.000000 76.4 ndhD-psaC_p4 ndhD-psaC 4 AAAATTAAGGAACCCGCGGA AAGAGATGTGAATCCGCCTG 20 20 45.0 50.0 57.8 57.7 30.7 0.0 0.525826 121547 121656 286-287 1.000000 76.4 ndhD-psaC_p5 ndhD-psaC 5 GAAAATTAAGGAACCCGCGG AGAGGACTGTGTCGGTTGTA 20 20 50.0 50.0 57.4 58.0 21.0 0.0 0.563685 121547 121656 306-307 1.000000 76.0 ndhA_p1 ndhA 1 ACCCAGTTACCATAGGGAACA CCCGCCGGGTTTTATCAATT 21 20 47.6 50.0 58.1 58.9 13.3 15.9 1.968148 124189 126362 2393-2393 1.000000 64.6 ndhA_p2 ndhA 2 ACCCAGTTACCATAGGGAACA CCGCCGGGTTTTATCAATTT 21 20 47.6 45.0 58.1 57.0 13.3 2.2 2.027550 124189 126362 2392-2392 1.000000 64.1 ndhA_p3 ndhA 3 TGAACCCAGTTACCATAGGGA CCCGCCGGGTTTTATCAATT 21 20 47.6 50.0 57.8 58.9 0.0 15.9 2.100625 124189 126362 2396-2396 1.000000 63.6 ndhA_p4 ndhA 4 ACCCAGTTACCATAGGGAACA GCCGGGTTTTATCAATTTGCA 21 21 47.6 42.9 58.1 58.0 13.3 2.2 2.110042 124189 126362 2390-2390 1.000000 63.5 ndhA_p5 ndhA 5 TGAACCCAGTTACCATAGGGA CCGCCGGGTTTTATCAATTT 21 20 47.6 45.0 57.8 57.0 0.0 2.2 2.160027 124189 126362 2395-2395 1.000000 63.1