primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGTAGCGAAGAACTTGCACC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.371395	3148	3880	1244-1244	0.500000	57.0
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGTAGCGAAGAACTTGCACC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.371395	3148	3880	1243-1243	0.500000	57.0
matK-trnK-UUU_p3	matK-trnK-UUU	3	TAGCGAAGAACTTGCACCAA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.8	58.2	0.0	17.1	0.416700	3148	3880	1242-1242	0.500000	56.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	TAGCGAAGAACTTGCACCAA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.8	58.2	0.0	17.1	0.416700	3148	3880	1241-1241	0.500000	56.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGTAGCGAAGAACTTGCACC	ATTTGAAGATGCGTGTGTGC	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.539530	3148	3880	1453-1453	0.500000	55.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCTTTCAGGATCAGTCGTG	AAGGCAGGCGTTTTTAAGGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026030	3918	4718	967-982	1.000000	85.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCCTTTCAGGATCAGTCGTG	AGGCAGGCGTTTTTAAGGAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026030	3918	4718	966-981	1.000000	85.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCAGGATCAGTCGTGG	AAGGCAGGCGTTTTTAAGGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026030	3918	4718	966-981	1.000000	85.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	AGGCAGGCGTTTTTAAGGAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026030	3918	4718	965-980	1.000000	85.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCCTTTCAGGATCAGTCGT	AGGCAGGCGTTTTTAAGGAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.071709	3918	4718	967-982	1.000000	85.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	5840	6192	433-539	1.000000	84.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5840	6192	426-532	1.000000	84.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCACACCGGATCATGTCTTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	9.5	16.9	0.189961	5840	6192	447-553	1.000000	83.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	0.0	16.9	0.210236	5840	6192	435-541	1.000000	83.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.293454	5840	6192	436-542	1.000000	83.2
atpF-atpH_p1	atpF-atpH	1	TGGCCCAAAGAAACGAAAGA	TTATGGCCTGGTTGTAGCAC	20	20	45.0	50.0	57.9	57.9	0.0	3.3	0.182833	12197	12483	367-377	1.000000	83.9
atpF-atpH_p2	atpF-atpH	2	GTGGCCCAAAGAAACGAAAG	TTATGGCCTGGTTGTAGCAC	20	20	50.0	50.0	57.9	57.9	0.0	3.3	0.256985	12197	12483	368-378	1.000000	83.3
atpF-atpH_p3	atpF-atpH	3	TGGCCCAAAGAAACGAAAGA	CCTGGTTGTAGCACTAGCAC	20	20	45.0	55.0	57.9	58.3	0.0	0.0	0.333197	12197	12483	361-371	1.000000	82.8
atpF-atpH_p4	atpF-atpH	4	AGTGGCCCAAAGAAACGAAA	TTATGGCCTGGTTGTAGCAC	20	20	45.0	50.0	58.2	57.9	0.0	3.3	0.352489	12197	12483	369-379	1.000000	82.5
atpF-atpH_p5	atpF-atpH	5	GTGGCCCAAAGAAACGAAAG	CCTGGTTGTAGCACTAGCAC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.407348	12197	12483	362-372	1.000000	82.2
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.111966	27205	27992	811-845	1.000000	85.6
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCGAGATTTACT	20	20	50.0	50.0	58.4	58.0	14.7	0.0	0.385329	27205	27992	829-863	1.000000	83.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTCCCTATTGTGTACCGTGC	CAGCCCAAGCGAGATTTACT	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.419800	27205	27992	874-908	1.000000	82.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	CCAGTTCAAATCCGGGTGTC	CAGCCCAAGCGAGATTTACT	20	20	55.0	50.0	58.8	58.0	0.0	0.0	0.864569	27205	27992	806-840	1.000000	79.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	27205	27992	818-852	1.000000	78.5
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	ATGAATTGCTGGTGGTAGAA	20	20	50.0	40.0	58.1	54.6	0.0	0.0	3.477004	43764	43943	261-264	1.000000	57.4
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	AGGCTTTTGTTTACCGAGGG	TGAATTGCTGGTGGTAGAAT	20	20	50.0	40.0	58.1	54.6	0.0	0.0	3.477004	43764	43943	260-263	1.000000	57.4
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GTAGGAAAGATGGCCGAGTG	AATGAATTGCTGGTGGTAGA	20	20	55.0	40.0	58.1	54.6	0.0	0.0	3.441317	43764	43943	312-315	1.000000	56.8
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GTAGGAAAGATGGCCGAGTG	ATGAATTGCTGGTGGTAGAA	20	20	55.0	40.0	58.1	54.6	0.0	0.0	3.441317	43764	43943	311-314	1.000000	56.8
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GTAGGAAAGATGGCCGAGTG	TGAATTGCTGGTGGTAGAAT	20	20	55.0	40.0	58.1	54.6	0.0	0.0	3.441317	43764	43943	310-313	1.000000	56.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGGTCCTTTGATAATCGGG	AGAGCCCAATGCACAAAAGA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.121399	48672	49825	1382-1387	1.000000	82.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGGTCCTTTGATAATCGGG	CGGTTCAAGTCCGTATAGCC	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.271950	48672	49825	1287-1292	1.000000	81.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGTCCTTTGATAATCGGGG	AGAGCCCAATGCACAAAAGA	20	20	55.0	45.0	57.7	58.0	0.0	0.0	0.272296	48672	49825	1381-1386	1.000000	80.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CGGGTCCTTTGATAATCGGG	GGTTCAAGTCCGTATAGCCC	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.356892	48672	49825	1286-1291	1.000000	80.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGGGTCCTTTGATAATCGGG	GGTCTTTGAAACAGGTCCGA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.370891	48672	49825	1353-1358	1.000000	80.2
accD-psaI_p1	accD-psaI	1	GGTGACAGCGAGTTTTGGTA	TGATTGCCGGAAATACGAGG	20	20	50.0	50.0	58.1	58.1	0.0	8.0	0.178198	57031	57777	1044-1064	1.000000	83.1
accD-psaI_p2	accD-psaI	2	GGTGACAGCGAGTTTTGGTA	TTGCTCGGACGGAAATAAGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.144844	57031	57777	1586-1644	1.000000	82.6
accD-psaI_p3	accD-psaI	3	ATGGGATCCGTAGTTGGTGA	TTGCTCGGACGGAAATAAGG	20	20	50.0	50.0	58.1	58.0	5.5	0.0	0.144412	57031	57777	1804-1804	0.500000	58.8
accD-psaI_p4	accD-psaI	4	ATGGGATCCGTAGTTGGTGA	TGATTGCCGGAAATACGAGG	20	20	50.0	50.0	58.1	58.1	5.5	8.0	0.177766	57031	57777	1282-1282	0.500000	58.6
accD-psaI_p5	accD-psaI	5	ATGGGATCCGTAGTTGGTGA	AAAAAGACCCGCAATCCCAT	20	20	50.0	45.0	58.1	58.1	5.5	0.0	0.188679	57031	57777	1861-1861	0.500000	58.5
petA-psbJ_p1	petA-psbJ	1	GATCCATTACGGGTCCAAGG	CATATTCCGGATTGGGGTCA	20	20	55.0	50.0	57.7	57.1	0.0	4.1	1.205294	61083	62096	1158-1163	1.000000	76.7
petA-psbJ_p2	petA-psbJ	2	GATCCATTACGGGTCCAAGG	TGGTTCATATTCCGGATTGGG	20	21	55.0	47.6	57.7	57.8	0.0	4.1	1.478290	61083	62096	1163-1168	1.000000	74.5
petA-psbJ_p3	petA-psbJ	3	GATCCATTACGGGTCCAAGG	GTTCATATTCCGGATTGGGGT	20	21	55.0	47.6	57.7	57.8	0.0	4.1	1.479791	61083	62096	1161-1166	1.000000	74.5
petA-psbJ_p4	petA-psbJ	4	GATCCATTACGGGTCCAAGG	TATTCCGGATTGGGGTCATC	20	20	55.0	50.0	57.7	56.8	0.0	4.1	1.500024	61083	62096	1156-1161	1.000000	74.4
petA-psbJ_p5	petA-psbJ	5	GATCCATTACGGGTCCAAGG	TCATATTCCGGATTGGGGTC	20	20	55.0	50.0	57.7	56.8	0.0	4.1	1.500024	61083	62096	1159-1164	1.000000	74.3
petL-petG_p1	petL-petG	1	TGAGCAAGATACGACTTATTTGA	GCCGCGCCTGTATTGTAAAT	23	20	34.8	50.0	55.8	59.3	0.0	0.0	6.550889	64060	64241	296-301	1.000000	45.1
petL-petG_p2	petL-petG	2	TGAGCAAGATACGACTTATTTG	GCCGCGCCTGTATTGTAAAT	22	20	36.4	50.0	54.3	59.3	0.0	0.0	7.003486	64060	64241	296-301	1.000000	45.1
petL-petG_p3	petL-petG	3	TGAGCAAGATACGACTTATTTGA	GCCGCGCCTGTATTGTAAA	23	19	34.8	52.6	55.8	58.9	0.0	0.0	7.120375	64060	64241	296-301	1.000000	45.1
petL-petG_p4	petL-petG	4	TGAGCAAGATACGACTTATTTGA	CCGCGCCTGTATTGTAAATA	23	20	34.8	45.0	55.8	56.0	0.0	0.0	7.190612	64060	64241	295-300	1.000000	45.1
petL-petG_p5	petL-petG	5	GAGCAAGATACGACTTATTTGA	GCCGCGCCTGTATTGTAAAT	22	20	36.4	50.0	54.1	59.3	0.0	0.0	7.268250	64060	64241	295-300	1.000000	45.1
infA-rps8_p1	infA-rps8	1	GGAAGTGGTTCAGGAATGAAC	CGACTAGAACGAATAGGCGG	21	20	47.6	55.0	56.8	58.0	11.7	11.8	2.232049	78125	78217	184-193	1.000000	65.1
infA-rps8_p2	infA-rps8	2	GGAAGTGGTTCAGGAATGAAC	GCGGTATGGGGGTTGTAATT	21	20	47.6	50.0	56.8	57.9	11.7	0.0	2.259765	78125	78217	243-252	1.000000	64.2
infA-rps8_p3	infA-rps8	3	GGAAGTGGTTCAGGAATGAAC	GAGGGATAATGACAGACCGC	21	20	47.6	55.0	56.8	57.8	11.7	0.0	2.354599	78125	78217	210-219	1.000000	63.8
infA-rps8_p4	infA-rps8	4	GGAAGTGGTTCAGGAATGAAC	TAGAACGAATAGGCGGGGAA	21	20	47.6	50.0	56.8	58.2	11.7	0.0	2.419035	78125	78217	180-189	1.000000	63.7
infA-rps8_p5	infA-rps8	5	GGAAGTGGTTCAGGAATGAAC	GGATAATGACAGACCGCGAG	21	20	47.6	55.0	56.8	58.2	11.7	8.2	2.426073	78125	78217	207-216	1.000000	63.2
rps8-rpl14_p1	rps8-rpl14	1	TCTATCCAGATCAGCATTTCGT	AATAGTGTCATTGGCTCCCG	22	20	40.9	50.0	57.1	57.7	0.0	0.0	3.276682	78623	78805	274-274	1.000000	57.6
rps8-rpl14_p2	rps8-rpl14	2	TCTATCCAGATCAGCATTTCGT	ATAGTGTCATTGGCTCCCGA	22	20	40.9	50.0	57.1	58.5	0.0	0.0	3.448559	78623	78805	273-273	1.000000	56.2
rps8-rpl14_p3	rps8-rpl14	3	TCTATCCAGATCAGCATTTCGT	GTCATTGGCTCCCGAAGTAT	22	20	40.9	50.0	57.1	57.4	0.0	0.0	3.566682	78623	78805	268-268	1.000000	55.3
rps8-rpl14_p4	rps8-rpl14	4	TTCTATCCAGATCAGCATTTCGT	AATAGTGTCATTGGCTCCCG	23	20	39.1	50.0	57.7	57.7	0.0	0.0	3.614118	78623	78805	275-275	1.000000	54.9
rps8-rpl14_p5	rps8-rpl14	5	TCTATCCAGATCAGCATTTCGT	TGTCATTGGCTCCCGAAGTA	22	20	40.9	50.0	57.1	58.7	0.0	0.0	3.672762	78623	78805	269-269	1.000000	54.5
ycf1_p1	ycf1	1	TAATTATTGAACCCGCGCGT	CTTAATACTCTCCGGTGCCG	20	20	45.0	55.0	58.3	57.9	29.9	0.0	0.481347	111221	111340	256-256	1.000000	79.3
ycf1_p2	ycf1	2	TAATTATTGAACCCGCGCGT	TTAATACTCTCCGGTGCCGA	20	20	45.0	50.0	58.3	58.2	29.9	12.7	0.579190	111221	111340	255-255	1.000000	78.5
ycf1_p3	ycf1	3	TAATTATTGAACCCGCGCGT	TGCCGAGATGCTAAAACCAT	20	20	45.0	45.0	58.3	57.6	29.9	0.0	0.764051	111221	111340	241-241	1.000000	77.2
ycf1_p4	ycf1	4	TTAATTATTGAACCCGCGCG	CTTAATACTCTCCGGTGCCG	20	20	45.0	55.0	57.3	57.9	25.7	0.0	0.876787	111221	111340	257-257	1.000000	76.1
ycf1_p5	ycf1	5	TAATTATTGAACCCGCGCGT	GTGCCGAGATGCTAAAACCA	20	20	45.0	50.0	58.3	58.6	29.9	0.0	0.896699	111221	111340	242-242	1.000000	76.1
ndhA-ndhI_p1	ndhA-ndhI	1	ACCAAGACTTAGAATGGATCAACT	TGACCAATATACCTTGCGGC	24	20	37.5	50.0	57.6	58.0	9.0	0.0	4.418285	115760	115845	264-266	1.000000	46.5
ndhA-ndhI_p2	ndhA-ndhI	2	ACCAAGACTTAGAATGGATCAACT	CAATATACCTTGCGGCTCGT	24	20	37.5	50.0	57.6	58.1	9.0	0.0	4.514214	115760	115845	260-262	1.000000	45.7
ndhA-ndhI_p3	ndhA-ndhI	3	ACCAAGACTTAGAATGGATCAACT	ACCAATATACCTTGCGGCTC	24	20	37.5	50.0	57.6	57.7	9.0	0.0	4.636196	115760	115845	262-264	1.000000	44.7
ndhA-ndhI_p4	ndhA-ndhI	4	ACCAAGACTTAGAATGGATCAACT	GACCAATATACCTTGCGGCT	24	20	37.5	50.0	57.6	57.7	9.0	0.0	4.636196	115760	115845	263-265	1.000000	44.7
ndhA-ndhI_p5	ndhA-ndhI	5	ACCAAGACTTAGAATGGATCAACT	GCTCGTACTGTCTGTTGACC	24	20	37.5	55.0	57.6	58.3	9.0	0.0	4.680736	115760	115845	246-248	1.000000	44.5
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	GGCGGCATTAAAAGCTTTGT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.086679	121175	121738	681-681	1.000000	84.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	GGCGGCATTAAAAGCTTTGT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.088049	121175	121738	682-682	1.000000	84.5
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AATAAACCCAACGGCCACTT	GGCGGCATTAAAAGCTTTGT	20	20	45.0	45.0	58.0	57.9	2.2	13.5	0.082785	121175	121738	1045-1046	1.000000	82.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AATAAACCCAACGGCCACTT	GGATACTCGGCGGCATTAAA	20	20	45.0	50.0	58.0	58.1	2.2	10.5	0.130239	121175	121738	1053-1054	1.000000	82.5
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCTGGTACCAATATTGCGG	GGCGGCATTAAAAGCTTTGT	20	20	50.0	45.0	58.0	57.9	17.1	13.5	0.111530	121175	121738	1248-1248	0.500000	59.1
rpl32-ndhF_p1	rpl32-ndhF	1	GGTCGATGGGGAAAAGACTC	ACATAGGAACGGGAAGTGGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.078604	121904	122871	1392-1463	1.000000	86.4
rpl32-ndhF_p2	rpl32-ndhF	2	GTCGATGGGGAAAAGACTCC	ACATAGGAACGGGAAGTGGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.078604	121904	122871	1391-1462	1.000000	86.4
rpl32-ndhF_p3	rpl32-ndhF	3	GTCGATGGGGAAAAGACTCC	AACCAAAATCCCCAACACGA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.100317	121904	122871	1895-1966	1.000000	85.7
rpl32-ndhF_p4	rpl32-ndhF	4	GGTCGATGGGGAAAAGACTC	TACATGAAGGGGGAATTGCG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.076923	121904	122871	2075-2146	1.000000	85.5
rpl32-ndhF_p5	rpl32-ndhF	5	GTCGATGGGGAAAAGACTCC	TACATGAAGGGGGAATTGCG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.076923	121904	122871	2074-2145	1.000000	85.5