primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	trnH-CAC-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	77	392	350-374	1.000000	84.9
trnH-CAC-psbA_p2	trnH-CAC-psbA	2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	77	392	365-389	1.000000	84.7
trnH-CAC-psbA_p3	trnH-CAC-psbA	3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	77	392	351-375	1.000000	84.7
trnH-CAC-psbA_p4	trnH-CAC-psbA	4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	392	368-392	1.000000	84.6
trnH-CAC-psbA_p5	trnH-CAC-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	77	392	366-390	1.000000	84.5
trnK-AAA-trnQ-CAA_p1	trnK-AAA-trnQ-CAA	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4352	5705	1412-2185	1.000000	90.0
trnK-AAA-trnQ-CAA_p2	trnK-AAA-trnQ-CAA	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4352	5705	1411-2184	1.000000	90.0
trnK-AAA-trnQ-CAA_p3	trnK-AAA-trnQ-CAA	3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4352	5705	1398-2171	1.000000	87.7
trnK-AAA-trnQ-CAA_p4	trnK-AAA-trnQ-CAA	4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	6.4	16.9	0.646638	4352	5705	1423-2196	1.000000	86.8
trnK-AAA-trnQ-CAA_p5	trnK-AAA-trnQ-CAA	5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4352	5705	1421-2194	1.000000	85.6
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.8	57.2	0.0	0.0	0.926353	6913	7543	741-755	1.000000	75.6
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	58.2	57.2	2.0	0.0	0.965511	6913	7543	739-753	1.000000	75.3
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.6	57.2	0.9	0.0	1.172484	6913	7543	736-750	1.000000	73.6
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.5	57.2	0.0	0.0	1.213145	6913	7543	742-756	1.000000	73.3
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	20	20	50.0	50.0	57.8	56.9	0.0	0.0	1.225207	6913	7543	740-754	1.000000	73.2
rpoB-trnC-UGC_p1	rpoB-trnC-UGC	1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.227525	25241	26334	1176-1182	1.000000	80.2
rpoB-trnC-UGC_p2	rpoB-trnC-UGC	2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	57.9	58.4	0.0	14.6	0.500888	25241	26334	1158-1164	1.000000	78.1
rpoB-trnC-UGC_p3	rpoB-trnC-UGC	3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.563463	25241	26334	1200-1206	1.000000	77.5
rpoB-trnC-UGC_p4	rpoB-trnC-UGC	4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.981431	25241	26334	1177-1183	1.000000	74.2
rpoB-trnC-UGC_p5	rpoB-trnC-UGC	5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	20	19	55.0	52.6	57.9	58.0	0.0	0.0	1.152069	25241	26334	1198-1204	1.000000	72.8
psbM-trnD-GAC_p1	psbM-trnD-GAC	1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.2	58.5	0.0	5.4	3.295722	27799	28826	1139-1152	1.000000	55.2
psbM-trnD-GAC_p2	psbM-trnD-GAC	2	AGAATGAAGAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	55.2	57.0	0.0	5.4	3.815876	27799	28826	1140-1153	1.000000	51.1
psbM-trnD-GAC_p3	psbM-trnD-GAC	3	AGAATGAAGAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	20	19	45.0	52.6	55.2	57.8	0.0	5.4	4.050125	27799	28826	1138-1151	1.000000	49.2
psbM-trnD-GAC_p4	psbM-trnD-GAC	4	AGAATGAAGAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	20	21	45.0	47.6	55.2	58.5	0.0	5.4	4.309865	27799	28826	1141-1154	1.000000	47.1
psbM-trnD-GAC_p5	psbM-trnD-GAC	5	TAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	55.2	58.5	0.0	5.4	4.320309	27799	28826	1140-1153	1.000000	47.0
trnT-ACC-psbD_p1	trnT-ACC-psbD	1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	20	20	55.0	50.0	58.1	57.9	7.6	0.0	0.198269	30439	31703	1405-1432	1.000000	82.0
trnT-ACC-psbD_p2	trnT-ACC-psbD	2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.220423	30439	31703	1408-1435	1.000000	81.8
trnT-ACC-psbD_p3	trnT-ACC-psbD	3	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.417007	30439	31703	1382-1409	1.000000	80.3
trnT-ACC-psbD_p4	trnT-ACC-psbD	4	TGGTAGAGTAACGCCATGGT	CAAAAACAAAGCGGTCCCTC	20	20	50.0	50.0	58.4	57.9	14.7	0.0	0.570277	30439	31703	1404-1431	1.000000	79.0
trnT-ACC-psbD_p5	trnT-ACC-psbD	5	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	20	20	55.0	55.0	58.1	58.5	7.6	0.0	0.582197	30439	31703	1391-1418	1.000000	78.9
psbC-trnS-UCA_p1	psbC-trnS-UCA	1	GAGCGACGTTCTGCAGAATA	ACGGGTATACTGGATCGTGT	20	20	50.0	50.0	58.1	57.9	30.7	2.3	0.153595	34135	34315	695-696	1.000000	80.5
psbC-trnS-UCA_p2	psbC-trnS-UCA	2	ACCGCTTACCCTAGTGAGTT	TGGGAGGGGTCATCTCATTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.079969	34135	34315	1396-1402	1.000000	80.4
psbC-trnS-UCA_p3	psbC-trnS-UCA	3	GAGCGACGTTCTGCAGAATA	TGGGAGGGGTCATCTCATTT	20	20	50.0	50.0	58.1	58.0	30.7	0.0	0.105003	34135	34315	1114-1120	1.000000	80.4
psbC-trnS-UCA_p4	psbC-trnS-UCA	4	ACCGCTTACCCTAGTGAGTT	ACGGGTATACTGGATCGTGT	20	20	50.0	50.0	58.1	57.9	0.0	2.3	0.128561	34135	34315	977-978	1.000000	80.2
psbC-trnS-UCA_p5	psbC-trnS-UCA	5	TATGGTCTGGGGAGGCTTAC	TGGGAGGGGTCATCTCATTT	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.211825	34135	34315	1485-1491	1.000000	79.3
rps4-trnT-ACA_p1	rps4-trnT-ACA	1	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137323	44704	45094	569-577	1.000000	81.0
rps4-trnT-ACA_p2	rps4-trnT-ACA	2	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245076	44704	45094	537-545	1.000000	80.3
rps4-trnT-ACA_p3	rps4-trnT-ACA	3	TCTAAGATCGCTTCCTGCCT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.350954	44704	45094	553-561	1.000000	79.3
rps4-trnT-ACA_p4	rps4-trnT-ACA	4	TCTAAGATCGCTTCCTGCCT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.458707	44704	45094	521-529	1.000000	78.6
rps4-trnT-ACA_p5	rps4-trnT-ACA	5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.0	57.5	0.0	7.6	0.505091	44704	45094	577-585	1.000000	78.0
trnL-UUA-trnF-UUC_p1	trnL-UUA-trnF-UUC	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	46566	47324	633-934	1.000000	83.8
trnL-UUA-trnF-UUC_p2	trnL-UUA-trnF-UUC	2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	46566	47324	630-931	1.000000	80.6
trnL-UUA-trnF-UUC_p3	trnL-UUA-trnF-UUC	3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	46566	47324	622-923	1.000000	80.0
trnL-UUA-trnF-UUC_p4	trnL-UUA-trnF-UUC	4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.079322	46566	47324	609-910	1.000000	79.5
trnL-UUA-trnF-UUC_p5	trnL-UUA-trnF-UUC	5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.079322	46566	47324	605-906	1.000000	79.5
ndhK-ndhC_p1	ndhK-ndhC	1	GGCTGGAAAGTCTTGACCAA	ATGGCGAAAGGGAGCATTAG	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.045871	49030	49096	192-198	1.000000	82.5
ndhK-ndhC_p2	ndhK-ndhC	2	GGCTGGAAAGTCTTGACCAA	GGCGAAAGGGAGCATTAGAA	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.057130	49030	49096	190-196	1.000000	82.4
ndhK-ndhC_p3	ndhK-ndhC	3	GGCTGGAAAGTCTTGACCAA	TTTATGCATGGCGAAAGGGA	20	20	50.0	45.0	58.0	57.9	11.3	10.2	0.155042	49030	49096	199-205	1.000000	81.5
ndhK-ndhC_p4	ndhK-ndhC	4	GGCTGGAAAGTCTTGACCAA	GTTTATGCATGGCGAAAGGG	20	20	50.0	50.0	58.0	57.8	11.3	10.2	0.227069	49030	49096	200-206	1.000000	80.9
ndhK-ndhC_p5	ndhK-ndhC	5	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	20	20	50.0	45.0	58.0	57.8	11.3	0.0	0.217570	49030	49096	281-287	1.000000	80.2
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	20	21	45.0	47.6	57.6	58.1	19.2	0.0	1.506956	61676	62585	1097-1152	1.000000	70.9
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	20	21	45.0	47.6	57.6	57.8	19.2	0.0	1.598556	61676	62585	1098-1153	1.000000	70.2
petA-psbJ_p3	petA-psbJ	3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	20	21	45.0	47.6	57.4	58.1	0.0	0.0	1.664061	61676	62585	1071-1126	1.000000	69.8
petA-psbJ_p4	petA-psbJ	4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	20	21	45.0	47.6	57.4	57.8	0.0	0.0	1.755661	61676	62585	1072-1127	1.000000	69.0
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.2	58.1	12.3	0.0	1.848592	61676	62585	1103-1158	1.000000	68.2
rps18-rpl20_p1	rps18-rpl20	1	GAAAGAAGTGAGTCGACCCC	AACCGGAAAATACTTGCACA	20	20	55.0	40.0	57.9	55.9	13.0	9.5	2.220447	67216	67487	404-407	1.000000	63.8
rps18-rpl20_p2	rps18-rpl20	2	GAAAGAAGTGAGTCGACCCC	ACCGGAAAATACTTGCACAA	20	20	55.0	40.0	57.9	55.9	13.0	9.5	2.220447	67216	67487	403-406	1.000000	63.8
rps18-rpl20_p3	rps18-rpl20	3	GAAGTGAGTCGACCCCTAGA	AACCGGAAAATACTTGCACA	20	20	55.0	40.0	57.9	55.9	13.0	9.5	2.264494	67216	67487	400-403	1.000000	63.5
rps18-rpl20_p4	rps18-rpl20	4	GAAGTGAGTCGACCCCTAGA	ACCGGAAAATACTTGCACAA	20	20	55.0	40.0	57.9	55.9	13.0	9.5	2.264494	67216	67487	399-402	1.000000	63.5
rps18-rpl20_p5	rps18-rpl20	5	AGAAGTGAGTCGACCCCTAG	ACCGGAAAATACTTGCACAA	20	20	55.0	40.0	57.9	55.9	13.0	9.5	2.270497	67216	67487	400-403	1.000000	63.4
petD_p1	petD	1	TCCTTCAACTCCACAGTATTGT	ACTCCCTAGATACCCACGTC	22	20	40.9	55.0	57.0	57.6	0.0	0.0	3.336287	75560	76752	1318-1323	1.000000	54.3
petD_p2	petD	2	TCCTTCAACTCCACAGTATTGT	CTCCCTAGATACCCACGTCT	22	20	40.9	55.0	57.0	57.3	0.0	0.0	3.635314	75560	76752	1317-1322	1.000000	51.9
petD_p3	petD	3	TTTTCCTTCAACTCCACAGT	ACTCCCTAGATACCCACGTC	20	20	40.0	55.0	54.7	57.6	0.0	0.0	3.661476	75560	76752	1321-1326	1.000000	51.7
petD_p4	petD	4	TTCCTTCAACTCCACAGTATTGT	ACTCCCTAGATACCCACGTC	23	20	39.1	55.0	57.7	57.6	0.0	0.0	3.666329	75560	76752	1319-1324	1.000000	51.7
petD_p5	petD	5	TTTTTCCTTCAACTCCACAGT	ACTCCCTAGATACCCACGTC	21	20	38.1	55.0	55.5	57.6	0.0	0.0	3.819418	75560	76752	1322-1327	1.000000	50.5
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	GCACTCTCGTACGGATTTCA	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	57.7	58.0	16.5	6.7	0.287896	112817	113604	1757-1762	1.000000	80.1
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	AAATGAGCACTCTCGTACGG	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	57.7	58.0	16.5	6.7	0.294109	112817	113604	1763-1768	1.000000	80.0
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	GCACTCTCGTACGGATTTCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.7	57.9	16.5	0.0	0.397647	112817	113604	1216-1221	1.000000	80.0
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	AAATGAGCACTCTCGTACGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.7	57.9	16.5	0.0	0.403860	112817	113604	1222-1227	1.000000	80.0
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	ATGAGCACTCTCGTACGGAT	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	58.3	58.0	16.5	6.7	0.332989	112817	113604	1761-1766	1.000000	79.7
ndhH_p1	ndhH	1	TCCATATTAGTCCGTAGACCTCT	TCATGCTAGAACGAATCAAGGA	23	22	43.5	40.9	57.4	57.3	0.0	0.0	6.315878	121329	122510	1297-1297	1.000000	40.6
ndhH_p2	ndhH	2	GGACAAACATCCATATTAGTCCG	TCATGCTAGAACGAATCAAGGA	23	22	43.5	40.9	57.1	57.3	0.0	0.0	6.660601	121329	122510	1306-1306	1.000000	40.6
ndhH_p3	ndhH	3	TCCATATTAGTCCGTAGACCTCT	TCATGCTAGAACGAATCAAGGAA	23	23	43.5	39.1	57.4	57.9	0.0	0.0	6.664570	121329	122510	1297-1297	1.000000	40.6
ndhH_p4	ndhH	4	TCCATATTAGTCCGTAGACCTCT	TTCATGCTAGAACGAATCAAGGA	23	23	43.5	39.1	57.4	57.9	0.0	0.0	6.664570	121329	122510	1298-1298	1.000000	40.6
ndhH_p5	ndhH	5	ACATCCATATTAGTCCGTAGACC	TCATGCTAGAACGAATCAAGGA	23	22	43.5	40.9	57.0	57.3	0.0	0.0	6.715413	121329	122510	1300-1300	1.000000	40.6
ycf1_p1	ycf1	1	GTTTTACGCCTTTGGGAACG	ACATGCCGAAGTGATGGAAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.239541	126574	126693	914-938	1.000000	81.1
ycf1_p2	ycf1	2	GTTTTACGCCTTTGGGAACG	AAAAGCACCCTCTGACCAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053832	126574	126693	810-834	0.500000	62.0
ycf1_p3	ycf1	3	GTTTTACGCCTTTGGGAACG	GAAAAAGCACCCTCTGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059458	126574	126693	812-836	0.500000	62.0
ycf1_p4	ycf1	4	GGACCACTCGGGAAATTGAA	AAAAGCACCCTCTGACCAAG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.207319	126574	126693	1168-1168	0.250000	48.3
ycf1_p5	ycf1	5	GGACCACTCGGGAAATTGAA	GAAAAAGCACCCTCTGACCA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.212945	126574	126693	1170-1170	0.250000	48.3