primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
orf490_p1	orf490	1	GGGGACCGATCTAAGGAAGA	TGTTCAAAGGCCTCTTGGTC	20	20	55.0	50.0	57.9	58.0	0.0	20.5	0.102372	102	1574	1973-1973	0.500000	59.2
orf490_p2	orf490	2	GGGGACCGATCTAAGGAAGA	GGCCTCTTGGTCTATTGCTC	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.123212	102	1574	1965-1965	0.500000	59.0
orf490_p3	orf490	3	GTGTTTCCTTCTTTGCAGGC	TGTTCAAAGGCCTCTTGGTC	20	20	50.0	50.0	57.9	58.0	0.0	20.5	0.158808	102	1574	1953-1953	0.500000	58.7
orf490_p4	orf490	4	GGGGACCGATCTAAGGAAGA	GGGCTTGTCGAACATACTGT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.178390	102	1574	1851-1851	0.500000	58.6
orf490_p5	orf490	5	GTGTTTCCTTCTTTGCAGGC	GGCCTCTTGGTCTATTGCTC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.179648	102	1574	1945-1945	0.500000	58.6
orf490-accD_p1	orf490-accD	1	CATACCGGTCAGTCACAAGG	CCATGCTGCCTCCCATAAAT	20	20	55.0	50.0	58.0	58.0	12.4	0.0	0.015515	1575	3518	2900-2900	0.500000	59.9
orf490-accD_p2	orf490-accD	2	CACCTGATCGATGAACTGCA	CCATGCTGCCTCCCATAAAT	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.019706	1575	3518	2701-2701	0.500000	59.8
orf490-accD_p3	orf490-accD	3	TGCACCTGATCGATGAACTG	CCATGCTGCCTCCCATAAAT	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.019706	1575	3518	2703-2703	0.500000	59.8
orf490-accD_p4	orf490-accD	4	ACCTGATCGATGAACTGCAC	CCATGCTGCCTCCCATAAAT	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.021106	1575	3518	2700-2700	0.500000	59.8
orf490-accD_p5	orf490-accD	5	AGCCAGACTCAACCAGTAGT	CCATGCTGCCTCCCATAAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022275	1575	3518	2419-2419	0.500000	59.8
ycf12-trnL(uaa)_p1	ycf12-trnL(uaa)	1	GTTCTGTCAGGTCCCCTAGT	TTGAGACATAGGTTCGCACG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.069221	6264	8209	2092-2092	0.500000	59.4
ycf12-trnL(uaa)_p2	ycf12-trnL(uaa)	2	TTCTGTCAGGTCCCCTAGTG	TTGAGACATAGGTTCGCACG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.070616	6264	8209	2091-2091	0.500000	59.4
ycf12-trnL(uaa)_p3	ycf12-trnL(uaa)	3	TGCACTCATCGTTCTGTCAG	TTGAGACATAGGTTCGCACG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079463	6264	8209	2102-2102	0.500000	59.4
ycf12-trnL(uaa)_p4	ycf12-trnL(uaa)	4	AGCTAGCTGTTCTTGCACTC	TTGAGACATAGGTTCGCACG	20	20	50.0	50.0	57.9	58.0	18.6	0.0	0.101053	6264	8209	2115-2115	0.500000	59.2
ycf12-trnL(uaa)_p5	ycf12-trnL(uaa)	5	CTAGTGATAGCCCTGCTTGC	TTGAGACATAGGTTCGCACG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.132879	6264	8209	2077-2077	0.500000	58.9
chlI-psbC_p1	chlI-psbC	1	GGTGCAAGAGAAGTTTAGCC	GCAGGTTTTGAGAAGGGGAT	20	20	50.0	50.0	56.7	57.8	0.0	0.0	1.505746	13605	15572	2080-2080	0.500000	48.0
chlI-psbC_p2	chlI-psbC	2	GGTGCAAGAGAAGTTTAGCCA	GCAGGTTTTGAGAAGGGGAT	21	20	47.6	50.0	58.5	57.8	0.0	0.0	1.717860	13605	15572	2080-2080	0.500000	46.3
chlI-psbC_p3	chlI-psbC	3	TGGTGCAAGAGAAGTTTAGCC	GCAGGTTTTGAGAAGGGGAT	21	20	47.6	50.0	58.5	57.8	0.0	0.0	1.717860	13605	15572	2081-2081	0.500000	46.3
chlI-psbC_p4	chlI-psbC	4	GGTGCAAGAGAAGTTTAGCC	GACACCGAACCCGTACTTTC	20	20	50.0	55.0	56.7	58.6	0.0	0.0	1.861131	13605	15572	2053-2053	0.500000	45.1
chlI-psbC_p5	chlI-psbC	5	GGTGCAAGAGAAGTTTAGCC	AACCCGTACTTTCTATGGGC	20	20	50.0	50.0	56.7	57.3	0.0	4.8	1.987983	13605	15572	2046-2046	0.500000	44.1
trnV(uac)-trnMe(cau)_p1	trnV(uac)-trnMe(cau)	1	GAGCTAGAAGGCCACAGAAC	TGAACCAATGACTTTCGCCT	20	20	55.0	45.0	58.0	57.7	5.0	0.0	0.287490	40783	41458	831-843	1.000000	81.5
trnV(uac)-trnMe(cau)_p2	trnV(uac)-trnMe(cau)	2	GGGCTATACGGATTCGAACC	TGAACCAATGACTTTCGCCT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.346331	40783	41458	803-815	1.000000	81.2
trnV(uac)-trnMe(cau)_p3	trnV(uac)-trnMe(cau)	3	ATGAGCTAGAAGGCCACAGA	TGAACCAATGACTTTCGCCT	20	20	50.0	45.0	58.1	57.7	5.0	0.0	0.398934	40783	41458	833-845	1.000000	80.6
trnV(uac)-trnMe(cau)_p4	trnV(uac)-trnMe(cau)	4	CGAACCGTAGACAATCTCGG	TGAACCAATGACTTTCGCCT	20	20	55.0	45.0	58.2	57.7	12.3	0.0	0.446524	40783	41458	789-801	1.000000	80.5
trnV(uac)-trnMe(cau)_p5	trnV(uac)-trnMe(cau)	5	AAGGCCACAGAACAAGGTTT	TGAACCAATGACTTTCGCCT	20	20	45.0	45.0	57.8	57.7	5.0	0.0	0.429531	40783	41458	824-836	1.000000	80.4
trnQ(uug)-chlB_p1	trnQ(uug)-chlB	1	AGCGGTAAAACACGTGACTT	GTCTCCTAAAGGTGCATGCA	20	20	45.0	50.0	58.1	57.9	25.0	18.5	0.169829	73389	73722	472-500	1.000000	86.8
trnQ(uug)-chlB_p2	trnQ(uug)-chlB	2	AAGCGGTAAAACACGTGACT	GTCTCCTAAAGGTGCATGCA	20	20	45.0	50.0	58.1	57.9	25.0	18.5	0.169829	73389	73722	473-501	1.000000	86.7
trnQ(uug)-chlB_p3	trnQ(uug)-chlB	3	GACTCATGCGTAGGAGGTTC	GTCTCCTAAAGGTGCATGCA	20	20	55.0	50.0	57.8	57.9	19.1	18.5	0.325213	73389	73722	451-479	1.000000	85.7
trnQ(uug)-chlB_p4	trnQ(uug)-chlB	4	AAGCGGTAAAACACGTGACT	AGTCGTCTCCTAAAGGTGCA	20	20	45.0	50.0	58.1	58.4	25.0	0.0	0.426926	73389	73722	477-505	1.000000	84.7
trnQ(uug)-chlB_p5	trnQ(uug)-chlB	5	AGCGGTAAAACACGTGACTT	AGTCGTCTCCTAAAGGTGCA	20	20	45.0	50.0	58.1	58.4	25.0	0.0	0.426926	73389	73722	476-504	1.000000	84.7
rpl19-trnF(gaa)_p1	rpl19-trnF(gaa)	1	AAGTGGGGCTTGCATAAACA	GGCAAACACTCGCACTTTAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040657	94319	94438	1075-1075	0.500000	59.7
rpl19-trnF(gaa)_p2	rpl19-trnF(gaa)	2	AGTGGGGCTTGCATAAACAA	GGCAAACACTCGCACTTTAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040657	94319	94438	1074-1074	0.500000	59.7
rpl19-trnF(gaa)_p3	rpl19-trnF(gaa)	3	AAGTGGGGCTTGCATAAACA	CTTCCTGCTGCTATTGCTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.042347	94319	94438	1112-1112	0.500000	59.7
rpl19-trnF(gaa)_p4	rpl19-trnF(gaa)	4	AGTGGGGCTTGCATAAACAA	CTTCCTGCTGCTATTGCTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.042347	94319	94438	1111-1111	0.500000	59.7
rpl19-trnF(gaa)_p5	rpl19-trnF(gaa)	5	GAGCGCATCCGATATCCAAT	GGCAAACACTCGCACTTTAC	20	20	50.0	50.0	58.0	58.0	11.5	0.0	0.048150	94319	94438	928-928	0.500000	59.6
orf340_p1	orf340	1	ATTGCTCGTAGGCTGTTCTG	AGCGTACCGACAACTTCATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	98436	99458	1785-1785	0.500000	59.8
orf340_p2	orf340	2	CATTGCTCGTAGGCTGTTCT	AGCGTACCGACAACTTCATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	98436	99458	1786-1786	0.500000	59.8
orf340_p3	orf340	3	ATTGCTCGTAGGCTGTTCTG	GGCTTGCGACTCACAATCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025881	98436	99458	2022-2022	0.500000	59.8
orf340_p4	orf340	4	CATTGCTCGTAGGCTGTTCT	GGCTTGCGACTCACAATCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025881	98436	99458	2023-2023	0.500000	59.8
orf340_p5	orf340	5	CCAAGCTCCCTCATACACAC	AGCGTACCGACAACTTCATC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037669	98436	99458	1909-1909	0.500000	59.7
orf340-trnI(gau)_p1	orf340-trnI(gau)	1	GCAATTGAGAGCGAAGGAGA	TTGAAGCCTACGTCATGCTC	20	20	50.0	50.0	58.0	58.0	10.3	6.8	0.025881	99459	100289	1842-1842	0.500000	59.8
orf340-trnI(gau)_p2	orf340-trnI(gau)	2	CTCAGCTTCGTAGTCGCTTT	TTGAAGCCTACGTCATGCTC	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.026173	99459	100289	1523-1523	0.500000	59.8
orf340-trnI(gau)_p3	orf340-trnI(gau)	3	CGAGATGCTGTTCGTGAAGA	TTGAAGCCTACGTCATGCTC	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.027533	99459	100289	1749-1749	0.500000	59.8
orf340-trnI(gau)_p4	orf340-trnI(gau)	4	GCAATTGAGAGCGAAGGAGA	AAGCCTACGTCATGCTCTTG	20	20	50.0	50.0	58.0	58.0	10.3	6.8	0.031472	99459	100289	1839-1839	0.500000	59.7
orf340-trnI(gau)_p5	orf340-trnI(gau)	5	GCAATTGAGAGCGAAGGAGA	CTTGAAGCCTACGTCATGCT	20	20	50.0	50.0	58.0	58.0	10.3	0.0	0.031472	99459	100289	1843-1843	0.500000	59.7
ndhB-minD_p1	ndhB-minD	1	CACTCTCGTTCGTAGTCTGC	ATCTTCTCTCTGTCCACGGT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.339047	113750	115622	1939-1939	0.500000	57.3
ndhB-minD_p2	ndhB-minD	2	TTGGTGTGAGCCCATTCATC	ATCTTCTCTCTGTCCACGGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.385691	113750	115622	1973-1973	0.500000	56.9
ndhB-minD_p3	ndhB-minD	3	AGGACACTCTCGTTCGTAGT	ATCTTCTCTCTGTCCACGGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.405757	113750	115622	1943-1943	0.500000	56.8
ndhB-minD_p4	ndhB-minD	4	GTAGTCTGCCTCTCTCGTGA	ATCTTCTCTCTGTCCACGGT	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.483507	113750	115622	1928-1928	0.500000	56.1
ndhB-minD_p5	ndhB-minD	5	ATTCATCTCCCTAGCCCAGG	ATCTTCTCTCTGTCCACGGT	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.484053	113750	115622	1960-1960	0.500000	56.1
minD-ccsA_p1	minD-ccsA	1	TCTTCTCGCTCTGGGAATCT	TCAATTGGTTGGGCAAAGGA	20	20	50.0	45.0	57.8	57.9	0.0	5.4	0.245602	116826	118070	1395-1407	1.000000	90.0
minD-ccsA_p2	minD-ccsA	2	TCTTCTCGCTCTGGGAATCT	AATTGGTTGGGCAAAGGACT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.253142	116826	118070	1393-1405	1.000000	90.0
minD-ccsA_p3	minD-ccsA	3	TCTTCTCGCTCTGGGAATCT	ATTGGTTGGGCAAAGGACTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.253142	116826	118070	1392-1404	1.000000	90.0
minD-ccsA_p4	minD-ccsA	4	TCTTCTCGCTCTGGGAATCT	TGGGCAAAGGACTTCATAGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.280273	116826	118070	1386-1398	1.000000	89.8
minD-ccsA_p5	minD-ccsA	5	TCTTCTCGCTCTGGGAATCT	CAATTGGTTGGGCAAAGGAC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.319828	116826	118070	1394-1406	1.000000	89.4
ycf1-chlN_p1	ycf1-chlN	1	TGTTGTTCGGTTCCACACAT	GGTTCCCATGCCTAGGATTG	20	20	45.0	55.0	58.0	58.0	0.0	14.8	0.058679	138697	139302	1326-1350	1.000000	84.1
ycf1-chlN_p2	ycf1-chlN	2	GAGGTGGGCGTTAGGTTTAG	GGTTCCCATGCCTAGGATTG	20	20	55.0	55.0	58.0	58.0	0.0	14.8	0.033065	138697	139302	1523-1547	1.000000	84.0
ycf1-chlN_p3	ycf1-chlN	3	GAGGTGGGCGTTAGGTTTAG	GGACAGCTACCACACTCATG	20	20	55.0	55.0	58.0	58.0	0.0	4.5	0.035683	138697	139302	1875-1899	1.000000	83.4
ycf1-chlN_p4	ycf1-chlN	4	GAGGTGGGCGTTAGGTTTAG	ATGCTCCGTGTTTGAGATCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.049786	138697	139302	1778-1802	1.000000	83.4
ycf1-chlN_p5	ycf1-chlN	5	GAGGTGGGCGTTAGGTTTAG	GGAGCAGATATGGAGGGGAT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.055962	138697	139302	1733-1757	1.000000	83.4
minD-ndhB_p1	minD-ndhB	1	ATCTTCTCTCTGTCCACGGT	CACTCTCGTTCGTAGTCTGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.339047	144084	145956	1939-1939	0.500000	57.3
minD-ndhB_p2	minD-ndhB	2	ATCTTCTCTCTGTCCACGGT	TTGGTGTGAGCCCATTCATC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.385691	144084	145956	1973-1973	0.500000	56.9
minD-ndhB_p3	minD-ndhB	3	ATCTTCTCTCTGTCCACGGT	AGGACACTCTCGTTCGTAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.405757	144084	145956	1943-1943	0.500000	56.8
minD-ndhB_p4	minD-ndhB	4	ATCTTCTCTCTGTCCACGGT	GTAGTCTGCCTCTCTCGTGA	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.483507	144084	145956	1928-1928	0.500000	56.1
minD-ndhB_p5	minD-ndhB	5	ATCTTCTCTCTGTCCACGGT	ATTCATCTCCCTAGCCCAGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.484053	144084	145956	1960-1960	0.500000	56.1
trnI(gau)-orf340_p1	trnI(gau)-orf340	1	TTGAAGCCTACGTCATGCTC	GCAATTGAGAGCGAAGGAGA	20	20	50.0	50.0	58.0	58.0	6.8	10.3	0.025881	159417	160247	1842-1842	0.500000	59.8
trnI(gau)-orf340_p2	trnI(gau)-orf340	2	TTGAAGCCTACGTCATGCTC	CTCAGCTTCGTAGTCGCTTT	20	20	50.0	50.0	58.0	58.0	6.8	0.0	0.026173	159417	160247	1523-1523	0.500000	59.8
trnI(gau)-orf340_p3	trnI(gau)-orf340	3	TTGAAGCCTACGTCATGCTC	CGAGATGCTGTTCGTGAAGA	20	20	50.0	50.0	58.0	58.0	6.8	0.0	0.027533	159417	160247	1749-1749	0.500000	59.8
trnI(gau)-orf340_p4	trnI(gau)-orf340	4	AAGCCTACGTCATGCTCTTG	GCAATTGAGAGCGAAGGAGA	20	20	50.0	50.0	58.0	58.0	6.8	10.3	0.031472	159417	160247	1839-1839	0.500000	59.7
trnI(gau)-orf340_p5	trnI(gau)-orf340	5	CTTGAAGCCTACGTCATGCT	GCAATTGAGAGCGAAGGAGA	20	20	50.0	50.0	58.0	58.0	0.0	10.3	0.031472	159417	160247	1843-1843	0.500000	59.7
trnF(gaa)-rpl19_p1	trnF(gaa)-rpl19	1	AGCCAATTGATAGCAGAGGC	TAAACCCCTTTGGCTCAACC	20	20	50.0	50.0	58.0	58.0	7.1	0.0	0.025482	163878	164877	1289-1289	0.500000	59.8
trnF(gaa)-rpl19_p2	trnF(gaa)-rpl19	2	AGCCAATTGATAGCAGAGGC	ACAACGAAAGACAAGCCCAT	20	20	50.0	45.0	58.0	58.0	7.1	0.0	0.040289	163878	164877	1694-1694	0.500000	59.7
trnF(gaa)-rpl19_p3	trnF(gaa)-rpl19	3	AGCCAATTGATAGCAGAGGC	GTAAAGTGCGAGTGTTTGCC	20	20	50.0	50.0	58.0	58.0	7.1	0.0	0.053960	163878	164877	1177-1177	0.500000	59.6
trnF(gaa)-rpl19_p4	trnF(gaa)-rpl19	4	AGCCAATTGATAGCAGAGGC	TGAGCAATAGCAGCAGGAAG	20	20	50.0	50.0	58.0	58.0	7.1	0.0	0.055650	163878	164877	1140-1140	0.500000	59.6
trnF(gaa)-rpl19_p5	trnF(gaa)-rpl19	5	AGCCAATTGATAGCAGAGGC	CTGAGATGGCCTGCTTACAG	20	20	50.0	55.0	58.0	58.0	7.1	3.3	0.070717	163878	164877	1397-1397	0.500000	59.4