primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	AAAACACCGAACCATCCGAT	AAAATGCAAGCACGGTTTGG	20	20	45.0	45.0	57.8	58.4	0.0	0.0	0.613386	1154	1390	448-449	1.000000	76.3
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	AAAATGCAAGCACGGTTTGG	20	20	45.0	45.0	57.8	58.4	0.0	0.0	0.662787	1154	1390	427-428	1.000000	76.0
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	AAAACACCGAACCATCCGAT	CTTCATCCGACTAGTTCCGG	20	20	45.0	55.0	57.8	57.5	0.0	15.1	0.687914	1154	1390	385-386	1.000000	75.9
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GCTTGTACTTTCGCGTCTCT	AAAATGCAAGCACGGTTTGG	20	20	50.0	45.0	58.3	58.4	5.8	0.0	0.719842	1154	1390	377-378	1.000000	75.7
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	CACCGAACCATCCGATGTAA	AAAATGCAAGCACGGTTTGG	20	20	50.0	45.0	57.7	58.4	7.6	0.0	0.722348	1154	1390	444-445	1.000000	75.5
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	20	22	45.0	40.9	58.0	57.3	0.0	0.0	2.757064	8598	9855	1335-1345	1.000000	63.2
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	20	22	45.0	40.9	58.0	57.0	0.0	0.0	3.015948	8598	9855	1336-1346	1.000000	61.1
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CGGAGAGAGAGGGATTCGAA	CGAACGAATCACACTTTTACCA	20	22	55.0	40.9	58.3	57.3	0.0	0.0	3.037320	8598	9855	1383-1393	1.000000	60.8
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.6	57.3	0.9	0.0	3.128806	8598	9855	1321-1331	1.000000	60.3
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	20	22	55.0	40.9	58.5	57.3	0.0	0.0	3.234820	8598	9855	1316-1326	1.000000	59.5
atpF_p1	atpF	1	TCGAAGAGTTACCATTAGTGTCTC	AGCTCTGTTCTTTGTTAGTCCT	24	22	41.7	40.9	57.8	57.0	0.0	0.0	7.157433	12546	14127	1716-1823	1.000000	42.8
atpF_p2	atpF	2	CGAAGAGTTACCATTAGTGTCTCT	AGCTCTGTTCTTTGTTAGTCCT	24	22	41.7	40.9	57.8	57.0	0.0	0.0	7.162563	12546	14127	1715-1822	1.000000	42.8
atpF_p3	atpF	3	TCGAAGAGTTACCATTAGTGTCT	AGCTCTGTTCTTTGTTAGTCCT	23	22	39.1	40.9	56.8	57.0	0.0	0.0	7.198642	12546	14127	1716-1823	1.000000	42.8
atpF_p4	atpF	4	TCGAAGAGTTACCATTAGTGTCTC	AGAGCTCTGTTCTTTGTTAGTCC	24	23	41.7	43.5	57.8	58.1	0.0	17.4	7.303317	12546	14127	1718-1825	1.000000	42.8
atpF_p5	atpF	5	TCGAAGAGTTACCATTAGTGTCTC	GAGCTCTGTTCTTTGTTAGTCCT	24	23	41.7	43.5	57.8	58.1	0.0	6.4	7.303317	12546	14127	1717-1824	1.000000	42.8
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	30051	31025	1127-1130	1.000000	70.0
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	30051	31025	1076-1079	1.000000	69.2
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.1	57.6	0.0	0.0	1.526483	30051	31025	1075-1078	1.000000	69.2
petN-psbM_p4	petN-psbM	4	ATGGTAAGTCTTGCTTGGGC	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.5	0.0	1.568000	30051	31025	1135-1138	1.000000	68.8
petN-psbM_p5	petN-psbM	5	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.8	57.6	0.0	0.0	1.583671	30051	31025	1074-1077	1.000000	68.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	20	20	45.0	55.0	55.8	58.1	0.0	0.0	2.287973	31131	32162	1128-1160	1.000000	65.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.315353	31131	32162	1113-1145	1.000000	64.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	20	20	45.0	50.0	55.8	58.1	0.0	10.4	2.374003	31131	32162	1132-1164	1.000000	64.4
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	22	20	40.9	55.0	57.4	58.1	0.0	0.0	2.682607	31131	32162	1135-1167	1.000000	61.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	22	20	40.9	50.0	57.4	57.9	0.0	0.0	2.709987	31131	32162	1120-1152	1.000000	61.7
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTGGCTGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.190415	37068	37194	239-240	1.000000	81.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	37068	37194	228-229	1.000000	81.4
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.213831	37068	37194	235-236	1.000000	81.3
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.213831	37068	37194	236-237	1.000000	81.3
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TTCCATGACCCCTCTTAGCT	GGTGGCTGAGTGGTTGATAG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.300272	37068	37194	231-232	1.000000	80.7
ndhJ-ndhK_p1	ndhJ-ndhK	1	GCAATCGAAGCCCAAAGATC	CAAGGTTCTTTTGCGCAGAA	20	20	50.0	45.0	57.5	57.5	2.1	11.3	0.972230	51101	51181	173-173	1.000000	73.1
ndhJ-ndhK_p2	ndhJ-ndhK	2	GAGTCCCAATCCTCGGTTTT	CAAGGTTCTTTTGCGCAGAA	20	20	50.0	45.0	57.5	57.5	0.0	11.3	0.982003	51101	51181	217-217	1.000000	72.9
ndhJ-ndhK_p3	ndhJ-ndhK	3	GGCAATCGAAGCCCAAAGAT	CAAGGTTCTTTTGCGCAGAA	20	20	50.0	45.0	58.9	57.5	18.6	11.3	1.384043	51101	51181	174-174	1.000000	69.8
ndhJ-ndhK_p4	ndhJ-ndhK	4	AGTCCCAATCCTCGGTTTTT	CAAGGTTCTTTTGCGCAGAA	20	20	45.0	45.0	57.0	57.5	0.0	11.3	1.462229	51101	51181	216-216	1.000000	69.0
ndhJ-ndhK_p5	ndhJ-ndhK	5	GCAATCGAAGCCCAAAGATC	CAAGGTTCTTTTGCGCAGAAT	20	21	50.0	42.9	57.5	58.0	2.1	14.3	1.499168	51101	51181	173-173	1.000000	68.9
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CCCAACCAGAAACTAGAGCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	74055	74489	611-611	1.000000	79.7
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	74055	74489	613-613	1.000000	79.7
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	74055	74489	531-531	1.000000	78.8
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	CCAACCAGAAACTAGAGCCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.274098	74055	74489	610-610	1.000000	78.5
clpP-psbB_p5	clpP-psbB	5	CTTTTGGAACACCAATGGGC	CCCAACCAGAAACTAGAGCC	20	20	50.0	55.0	57.8	57.9	3.6	0.0	0.283669	74055	74489	557-557	1.000000	78.4
rpl22_p1	rpl22	1	CCAAGTCTGAAACCAAGTGGA	ATTCATTGGCGGGGGATAAC	21	20	47.6	50.0	58.1	58.0	3.8	0.0	1.073674	85475	85861	495-502	1.000000	73.8
rpl22_p2	rpl22	2	CCAAGTCTGAAACCAAGTGGA	CATTCATTGGCGGGGGATAA	21	20	47.6	50.0	58.1	58.0	3.8	0.0	1.075094	85475	85861	496-503	1.000000	73.8
rpl22_p3	rpl22	3	CCAAGTCTGAAACCAAGTGGA	TTATCATTCATTGGCGGGGG	21	20	47.6	50.0	58.1	58.0	3.8	0.0	1.075094	85475	85861	500-507	1.000000	73.8
rpl22_p4	rpl22	4	CCAAGTCTGAAACCAAGTGGA	TCTGACTTTCGCGAGACATG	21	20	47.6	50.0	58.1	58.0	3.8	9.6	1.086438	85475	85861	570-576	1.000000	73.5
rpl22_p5	rpl22	5	CCAAGTCTGAAACCAAGTGGA	CCTACTCTGACTTTCGCGAG	21	20	47.6	55.0	58.1	57.8	3.8	9.6	1.250475	85475	85861	575-581	1.000000	72.2
trnI-GAU_p1	trnI-GAU	1	AGAGAGGGATGGGGTTTCTC	ATTCTTTATCCCTGGCGCAG	20	20	55.0	50.0	58.1	58.0	0.0	10.8	0.140706	104964	105977	1097-1099	1.000000	79.3
trnI-GAU_p2	trnI-GAU	2	AGAGGGATGGGGTTTCTCTC	ATTCTTTATCCCTGGCGCAG	20	20	55.0	50.0	58.1	58.0	0.0	10.8	0.140706	104964	105977	1095-1097	1.000000	79.3
trnI-GAU_p3	trnI-GAU	3	GAGAGGGATGGGGTTTCTCT	ATTCTTTATCCCTGGCGCAG	20	20	55.0	50.0	58.1	58.0	0.0	10.8	0.140706	104964	105977	1096-1098	1.000000	79.3
trnI-GAU_p4	trnI-GAU	4	GATGGGGTTTCTCTCGCTTT	ATTCTTTATCCCTGGCGCAG	20	20	50.0	50.0	57.9	58.0	0.0	10.8	0.142471	104964	105977	1090-1092	1.000000	79.3
trnI-GAU_p5	trnI-GAU	5	TCTCTCGCTTTTGGCATAGC	ATTCTTTATCCCTGGCGCAG	20	20	50.0	50.0	58.3	58.0	11.0	10.8	0.370476	104964	105977	1081-1083	1.000000	77.5
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	110759	111360	743-758	1.000000	78.7
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	110759	111360	780-795	1.000000	78.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110759	111360	694-709	1.000000	77.5
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	20	20	55.0	55.0	57.8	57.8	18.9	4.4	0.421214	110759	111360	695-710	1.000000	77.1
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.7	58.2	18.7	18.9	0.442458	110759	111360	731-746	1.000000	76.9
ndhF_p1	ndhF	1	GGGTATTGGGAAAAGAAGAGCA	GCGATAAGAATTGGTGAATCGG	22	22	45.5	45.5	58.3	57.9	0.0	0.0	4.436690	112712	114934	2325-2362	1.000000	45.8
ndhF_p2	ndhF	2	TGGGTATTGGGAAAAGAAGAGC	GCGATAAGAATTGGTGAATCGG	22	22	45.5	45.5	58.3	57.9	0.0	0.0	4.436690	112712	114934	2326-2363	1.000000	45.8
ndhF_p3	ndhF	3	GGGTATTGGGAAAAGAAGAGC	GCGATAAGAATTGGTGAATCGG	21	22	47.6	45.5	56.6	57.9	0.0	0.0	4.562698	112712	114934	2325-2362	1.000000	44.8
ndhF_p4	ndhF	4	GGGTATTGGGAAAAGAAGAGCA	CGAAAGCGATAAGAATTGGTGA	22	22	45.5	40.9	58.3	57.1	0.0	0.0	5.160705	112712	114934	2330-2367	1.000000	41.3
ndhF_p5	ndhF	5	GGGTATTGGGAAAAGAAGAGCA	TCGAAAGCGATAAGAATTGGTG	22	22	45.5	40.9	58.3	57.1	0.0	0.0	5.160705	112712	114934	2331-2368	1.000000	41.3
ccsA_p1	ccsA	1	TAGACACGCTGCTCTTAGGA	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.140013	116972	117937	1699-1700	1.000000	80.0
ccsA_p2	ccsA	2	TAGACACGCTGCTCTTAGGA	TTCTTGGTGGGAACGAGTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.162484	116972	117937	1801-1802	1.000000	79.8
ccsA_p3	ccsA	3	TAGACACGCTGCTCTTAGGA	CTCGATGGCTTCTCTTGCAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.173449	116972	117937	1697-1698	1.000000	79.7
ccsA_p4	ccsA	4	TAGACACGCTGCTCTTAGGA	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	57.9	58.1	0.0	15.1	0.196790	116972	117937	1683-1684	1.000000	79.6
ccsA_p5	ccsA	5	TAGACACGCTGCTCTTAGGA	GAATGAGTGGTTTCGTTGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.173806	116972	117937	1671-1671	0.500000	58.6
ccsA-ndhD_p1	ccsA-ndhD	1	CGAATTCGGCACTTGTAGCT	TGACAAAGTGCAGGCTATCC	20	20	50.0	50.0	58.6	57.9	20.2	0.0	0.754013	117938	118168	393-394	1.000000	77.4
ccsA-ndhD_p2	ccsA-ndhD	2	ATTCGGCACTTGTAGCTTCT	TGACAAAGTGCAGGCTATCC	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.884024	117938	118168	390-391	1.000000	76.4
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCACTTGTAGC	TGACAAAGTGCAGGCTATCC	20	20	50.0	50.0	58.9	57.9	29.8	0.0	1.037528	117938	118168	394-395	1.000000	75.1
ccsA-ndhD_p4	ccsA-ndhD	4	CGAATTCGGCACTTGTAGCT	GACAAAGTGCAGGCTATCCT	20	20	50.0	50.0	58.6	57.6	20.2	0.0	1.050101	117938	118168	392-393	1.000000	75.1
ccsA-ndhD_p5	ccsA-ndhD	5	CGAATTCGGCACTTGTAGCT	CGGATTTCGTTCTCTCGCTA	20	20	50.0	50.0	58.6	57.5	20.2	0.0	1.110348	117938	118168	418-419	1.000000	74.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	GGAAGCAGCTATTGGGCTAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.236326	120038	120516	666-672	1.000000	82.9
psaC-ndhE_p2	psaC-ndhE	2	CAATACATCTGTGGGGCAGG	GGAAGCAGCTATTGGGCTAG	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.426512	120038	120516	642-648	1.000000	81.5
psaC-ndhE_p3	psaC-ndhE	3	CCAATACATCTGTGGGGCAG	GGAAGCAGCTATTGGGCTAG	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.426512	120038	120516	643-649	1.000000	81.5
psaC-ndhE_p4	psaC-ndhE	4	CAGGCTCGTACACATTGAGT	GGAAGCAGCTATTGGGCTAG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.480736	120038	120516	626-632	1.000000	81.2
psaC-ndhE_p5	psaC-ndhE	5	AGCTTTACATCCGTCCCAAG	GGAAGCAGCTATTGGGCTAG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.513707	120038	120516	672-678	1.000000	80.6
ycf1_p1	ycf1	1	TGATTGGTATGATCCACGGT	ACGCATATCGACACCAAGAT	20	20	45.0	45.0	56.3	57.1	0.0	0.0	2.579289	127800	127919	124-253	1.000000	63.8
ycf1_p2	ycf1	2	ACGCATCAAAAAGTAGCACA	ACGCATATCGACACCAAGAT	20	20	40.0	45.0	56.3	57.1	0.0	0.0	2.618217	127800	127919	94-223	1.000000	63.6
ycf1_p3	ycf1	3	TGATTGGTATGATCCACGGT	GGACCAATACGCATATCGACA	20	21	45.0	47.6	56.3	58.0	0.0	0.0	2.709750	127800	127919	132-261	1.000000	62.7
ycf1_p4	ycf1	4	TGATTGGTATGATCCACGGT	TGGACCAATACGCATATCGAC	20	21	45.0	47.6	56.3	58.0	0.0	0.0	2.709750	127800	127919	133-262	1.000000	62.7
ycf1_p5	ycf1	5	ACGCATCAAAAAGTAGCACA	GGACCAATACGCATATCGACA	20	21	40.0	47.6	56.3	58.0	0.0	0.0	2.748678	127800	127919	102-231	1.000000	62.5
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	132577	133178	743-758	1.000000	78.7
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	132577	133178	780-795	1.000000	78.1
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	132577	133178	694-709	1.000000	77.5
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	57.8	57.8	4.4	18.9	0.421214	132577	133178	695-710	1.000000	77.1
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	20	20	50.0	55.0	58.2	57.7	18.9	18.7	0.442458	132577	133178	731-746	1.000000	76.9