primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	20	22	45.0	40.9	58.0	57.3	0.0	0.0	2.757064	8626	9887	1321-1339	1.000000	64.8
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	20	22	45.0	40.9	58.0	57.0	0.0	0.0	3.015948	8626	9887	1322-1340	1.000000	62.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.6	57.3	0.9	0.0	3.128806	8626	9887	1307-1325	1.000000	61.9
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	20	22	55.0	40.9	58.5	57.3	0.0	0.0	3.234820	8626	9887	1302-1320	1.000000	61.1
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GCTTTAGTCCACTCAGCCAT	TCGAACGAATCACACTTTTACC	20	22	50.0	40.9	57.6	57.0	0.9	0.0	3.387690	8626	9887	1308-1326	1.000000	59.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	GGTCGGTAATGCTGGTGAAT	20	20	45.0	50.0	56.4	58.0	0.0	0.0	1.622558	45791	46431	809-854	1.000000	71.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	GGTCGGTAATGCTGGTGAAT	20	20	45.0	50.0	56.4	58.0	0.0	0.0	1.622558	45791	46431	810-855	1.000000	71.7
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45791	46431	781-826	1.000000	70.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45791	46431	782-827	1.000000	70.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	45791	46431	730-775	1.000000	70.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47816	48757	992-1140	1.000000	81.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47816	48757	1000-1148	1.000000	81.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47816	48757	1024-1172	1.000000	81.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47816	48757	1032-1180	1.000000	80.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47816	48757	1015-1163	1.000000	80.1
atpB-rbcL_p1	atpB-rbcL	1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	20	20	45.0	45.0	57.9	56.6	2.1	0.0	1.507995	56270	57206	1030-1045	1.000000	72.9
atpB-rbcL_p2	atpB-rbcL	2	CAGGACCGGAAGTAGTAGGA	AGTCTCTGTTTGTGGTGACA	20	20	55.0	45.0	57.6	56.6	9.5	0.0	1.803650	56270	57206	974-989	1.000000	70.8
atpB-rbcL_p3	atpB-rbcL	3	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	57.5	56.6	8.0	0.0	1.868803	56270	57206	1031-1046	1.000000	70.0
atpB-rbcL_p4	atpB-rbcL	4	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	20	21	45.0	42.9	57.9	57.2	2.1	0.0	1.982079	56270	57206	1030-1045	1.000000	69.1
atpB-rbcL_p5	atpB-rbcL	5	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	58.7	56.6	0.0	0.0	2.038520	56270	57206	1054-1069	1.000000	68.5
rpoA-rps11_p1	rpoA-rps11	1	CGGGTGGATCCTGCTATTTC	CATTACGGGCCATTCGTAGA	20	20	55.0	50.0	58.1	57.5	14.2	4.4	0.635141	81004	81071	204-204	1.000000	76.1
rpoA-rps11_p2	rpoA-rps11	2	CGGGTGGATCCTGCTATTTC	TACGGGCCATTCGTAGAAGT	20	20	55.0	50.0	58.1	58.5	14.2	4.4	0.638211	81004	81071	201-201	1.000000	76.1
rpoA-rps11_p3	rpoA-rps11	3	CCTTTCATGAGTGGGGACAG	CATTACGGGCCATTCGTAGA	20	20	55.0	50.0	57.9	57.5	0.0	4.4	0.639178	81004	81071	291-291	1.000000	75.7
rpoA-rps11_p4	rpoA-rps11	4	CCTTTCATGAGTGGGGACAG	TACGGGCCATTCGTAGAAGT	20	20	55.0	50.0	57.9	58.5	0.0	4.4	0.642248	81004	81071	288-288	1.000000	75.7
rpoA-rps11_p5	rpoA-rps11	5	CGGGTGGATCCTGCTATTTC	TTACGGGCCATTCGTAGAAG	20	20	55.0	50.0	58.1	57.4	14.2	4.4	0.705562	81004	81071	202-202	1.000000	75.5
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	86501	86547	197-197	1.000000	79.6
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	86501	86547	183-183	1.000000	79.6
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86501	86547	182-182	1.000000	79.6
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86501	86547	184-184	1.000000	79.6
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.1	58.2	0.2	35.0	0.281592	86501	86547	211-211	1.000000	78.5
ycf1_p1	ycf1	1	CCGCCCAGTTTATCGACTTT	AGCCACCTCCTCTTCTTCAT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.301852	113524	113673	843-843	0.500000	57.6
ycf1_p2	ycf1	2	CCGCCCAGTTTATCGACTTT	CCACTCGGGGAATTGCAATA	20	20	50.0	50.0	58.3	57.9	0.0	13.4	0.317172	113524	113673	1305-1305	0.500000	57.5
ycf1_p3	ycf1	3	CCGCCCAGTTTATCGACTTT	CGTATCAAAGCCACCTCCTC	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.324512	113524	113673	851-851	0.500000	57.4
ycf1_p4	ycf1	4	CCGCCCAGTTTATCGACTTT	TGATTATACCGCGGCGAAAA	20	20	50.0	45.0	58.3	58.1	0.0	28.6	0.328559	113524	113673	889-889	0.500000	57.4
ycf1_p5	ycf1	5	CCGCCCAGTTTATCGACTTT	TTGATTATACCGCGGCGAAA	20	20	50.0	45.0	58.3	58.1	0.0	28.6	0.328559	113524	113673	890-890	0.500000	57.4
ndhH_p1	ndhH	1	TCATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	24	20	29.2	45.0	55.5	58.0	0.0	5.8	6.490568	118064	119259	1291-1291	0.500000	20.0
ndhH_p2	ndhH	2	TGAAGATTGGTTTGAATTTTGTTCT	TAATTTTGTCGCGTCCGCTA	25	20	28.0	45.0	56.4	58.0	0.0	5.8	6.638764	118064	119259	1349-1349	0.500000	20.0
ndhH_p3	ndhH	3	TTCATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	25	20	28.0	45.0	56.2	58.0	0.0	5.8	6.816045	118064	119259	1292-1292	0.500000	20.0
ndhH_p4	ndhH	4	CATTTTGATGAACCTCATTTTGA	TAATTTTGTCGCGTCCGCTA	23	20	30.4	45.0	54.1	58.0	0.0	5.8	6.906081	118064	119259	1290-1290	0.500000	20.0
ndhH_p5	ndhH	5	TCATTTTGATGAACCTCATTTTG	TAATTTTGTCGCGTCCGCTA	23	20	30.4	45.0	54.1	58.0	0.0	5.8	6.906081	118064	119259	1291-1291	0.500000	20.0
ndhA_p1	ndhA	1	GGGCATTATTGGTGGAGAGG	GCTCGTATTGTTTGTTGGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035929	119049	121255	2705-2705	0.500000	59.7
ndhA_p2	ndhA	2	TGGGAAGTCCAATGGCAAAA	GCTCGTATTGTTTGTTGGCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.116912	119049	121255	2584-2584	0.500000	59.1
ndhA_p3	ndhA	3	TGGGGTTTATCAGGACCGAT	GCTCGTATTGTTTGTTGGCC	20	20	50.0	50.0	58.1	58.0	4.6	0.0	0.135015	119049	121255	2674-2674	0.500000	58.9
ndhA_p4	ndhA	4	CTTGGACCTTTTATGGCGGA	GCTCGTATTGTTTGTTGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.145595	119049	121255	2976-2976	0.500000	58.8
ndhA_p5	ndhA	5	GCTACGAGCTTCTGGAATCC	GCTCGTATTGTTTGTTGGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.163429	119049	121255	2654-2654	0.500000	58.7
ndhE-rpl32_p1	ndhE-rpl32	1	TCAACTCGTATCAATCAATCT	AGAAAAAGCTTTTATGGCAGC	21	21	33.3	38.1	52.4	55.6	0.0	24.1	9.943478	123268	124080	951-951	0.500000	20.0
ndhE-rpl32_p2	ndhE-rpl32	2	TCAACTCGTATCAATCAATCT	AAGAAAAAGCTTTTATGGCAGC	21	22	33.3	36.4	52.4	56.4	0.0	24.1	10.198804	123268	124080	952-952	0.500000	20.0
ndhE-rpl32_p3	ndhE-rpl32	3	TCAACTCGTATCAATCAATCT	TGGCAGCTAAATATCCCTTT	21	20	33.3	40.0	52.4	54.4	0.0	0.0	10.236269	123268	124080	937-937	0.500000	20.0
ndhE-rpl32_p4	ndhE-rpl32	4	ATCAACTCGTATCAATCAATCT	AGAAAAAGCTTTTATGGCAGC	22	21	31.8	38.1	53.1	55.6	0.0	24.1	10.265981	123268	124080	952-952	0.500000	20.0
ndhE-rpl32_p5	ndhE-rpl32	5	TCAACTCGTATCAATCAATCT	TGGCAGCTAAATATCCCTTTT	21	21	33.3	38.1	52.4	55.2	0.0	0.0	10.369627	123268	124080	937-937	0.500000	20.0
psaC-ndhE_p1	psaC-ndhE	1	TACATCCGTCCCAGGGTATC	GGGTCACTTCACTGGTTTGT	20	20	55.0	50.0	58.0	57.9	5.0	0.0	0.058152	128337	128857	1289-1352	1.000000	86.2
psaC-ndhE_p2	psaC-ndhE	2	TACATCCGTCCCAGGGTATC	AGGCGCAATTACTATGGCTC	20	20	55.0	50.0	58.0	58.1	5.0	10.8	0.121135	128337	128857	1078-1141	1.000000	86.1
psaC-ndhE_p3	psaC-ndhE	3	TACATCCGTCCCAGGGTATC	GTTGGAGATGGGGTCACTTC	20	20	55.0	55.0	58.0	57.9	5.0	0.0	0.120713	128337	128857	1299-1362	1.000000	85.7
psaC-ndhE_p4	psaC-ndhE	4	TTTCTAGTAACCGGCCAAGC	GGGTCACTTCACTGGTTTGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.156319	128337	128857	1886-1950	1.000000	84.8
psaC-ndhE_p5	psaC-ndhE	5	TACATCCGTCCCAGGGTATC	GGAAGCAGCTATTGGGCTAG	20	20	55.0	55.0	58.0	58.1	5.0	0.0	0.115826	128337	128857	715-715	0.500000	59.1
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	165578	165624	197-197	1.000000	79.6
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	165578	165624	183-183	1.000000	79.6
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	165578	165624	182-182	1.000000	79.6
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	165578	165624	184-184	1.000000	79.6
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	20	20	55.0	55.0	58.2	58.1	35.0	0.2	0.281592	165578	165624	211-211	1.000000	78.5