primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTTCTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	5916	6624	744-789	1.000000	84.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GTTCTTCAAGTCGCACGTTG	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.176209	5916	6624	774-819	1.000000	83.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	ATGTTCTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	0.0	16.9	0.210236	5916	6624	746-791	1.000000	83.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5916	6624	737-782	1.000000	83.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CATGTTCTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.293454	5916	6624	747-792	1.000000	82.9
rpoC1-rpoB_p1	rpoC1-rpoB	1	TGGCCCAAGCACTTATTTGT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108847	23211	23242	181-181	1.000000	79.6
rpoC1-rpoB_p2	rpoC1-rpoB	2	TGTGGCCCAAGCACTTATTT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108847	23211	23242	183-183	1.000000	79.6
rpoC1-rpoB_p3	rpoC1-rpoB	3	TTGTGGCCCAAGCACTTATT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108847	23211	23242	184-184	1.000000	79.6
rpoC1-rpoB_p4	rpoC1-rpoB	4	TTTGTGGCCCAAGCACTTAT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108847	23211	23242	185-185	1.000000	79.6
rpoC1-rpoB_p5	rpoC1-rpoB	5	GGTAGGATTTTTGTGGCCCA	ACGATCTTTGGCTCTGGAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.168969	23211	23242	194-194	1.000000	79.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GAGGTTATAGTTAAAGCCGCT	20	21	50.0	42.9	57.9	55.5	6.4	0.0	3.561071	66355	67508	1314-1319	1.000000	52.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GAGGTTATAGTTAAAGCCGCT	20	21	50.0	42.9	57.8	55.5	24.9	0.0	3.666079	66355	67508	1237-1242	1.000000	51.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GAGGTTATAGTTAAAGCCGCT	20	21	45.0	42.9	58.2	55.5	0.0	0.0	3.680757	66355	67508	1323-1328	1.000000	51.1
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GAGGTTATAGTTAAAGCCGCT	20	21	50.0	42.9	58.2	55.5	0.0	0.0	3.693662	66355	67508	1328-1333	1.000000	51.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GAGGTTATAGTTAAAGCCGCT	20	21	50.0	42.9	57.8	55.5	0.0	0.0	3.697087	66355	67508	1322-1327	1.000000	51.0
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	TGTCAACGCATCTGGGAAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.125490	68388	68824	585-606	1.000000	82.2
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	TGTCAACGCATCTGGGAAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.126833	68388	68824	584-605	1.000000	82.2
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	TGTCAACGCATCTGGGAAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.133526	68388	68824	596-617	1.000000	82.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	TGTCAACGCATCTGGGAAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.134859	68388	68824	595-616	1.000000	82.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	TGTCAACGCATCTGGGAAAA	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.134859	68388	68824	598-619	1.000000	82.1
rpl22-rps19_p1	rpl22-rps19	1	TACTCGTCGTGCTTTGTGAG	CACCGACTCTAAACTTCCGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.054113	85914	85949	197-197	1.000000	80.0
rpl22-rps19_p2	rpl22-rps19	2	TACTCGTCGTGCTTTGTGAG	GGAGAATTTGCACCGACTCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.146887	85914	85949	207-207	1.000000	79.2
rpl22-rps19_p3	rpl22-rps19	3	TACTCGTCGTGCTTTGTGAG	ACGGATCGTATGGTAGGTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.399639	85914	85949	234-234	1.000000	77.1
rpl22-rps19_p4	rpl22-rps19	4	TACTCGTCGTGCTTTGTGAG	TGCACCGACTCTAAACTTCC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.496892	85914	85949	199-199	1.000000	76.4
rpl22-rps19_p5	rpl22-rps19	5	TTACTCGTCGTGCTTTGTGA	CACCGACTCTAAACTTCCGG	20	20	45.0	55.0	57.5	58.0	0.0	0.0	0.503239	85914	85949	198-198	1.000000	76.4
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CCTAACAGACCGATAGACTTGA	ACGCCTAGGACACCAGAATA	22	20	45.5	50.0	56.8	57.8	0.0	14.8	3.403490	108696	108951	300-300	1.000000	56.2
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CCTAACAGACCGATAGACTTGA	TACGCCTAGGACACCAGAAT	22	20	45.5	50.0	56.8	57.8	0.0	14.8	3.403490	108696	108951	301-301	1.000000	56.2
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	CCTAACAGACCGATAGACTTGA	GTTCCTCTACGCCTAGGACA	22	20	45.5	55.0	56.8	58.2	0.0	14.8	3.488731	108696	108951	308-308	1.000000	55.5
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	CCTAACAGACCGATAGACTTGA	TTCCTCTACGCCTAGGACAC	22	20	45.5	55.0	56.8	58.2	0.0	14.8	3.488731	108696	108951	307-307	1.000000	55.5
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	ACAGACCGATAGACTTGAAC	ACGCCTAGGACACCAGAATA	20	20	45.0	50.0	54.5	57.8	0.0	14.8	3.608241	108696	108951	296-296	1.000000	54.6
rrn5_p1	rrn5	1	ACCTGGGGCTGTAGTATGTT	AATGGTGCATTCCAAGGTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047165	108952	109071	984-984	0.500000	59.6
rrn5_p2	rrn5	2	TGGGGCTGTAGTATGTTCCA	AATGGTGCATTCCAAGGTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054300	108952	109071	981-981	0.500000	59.6
rrn5_p3	rrn5	3	GTCAAGTGGAAGTGCAGTGA	AATGGTGCATTCCAAGGTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.060451	108952	109071	571-571	0.500000	59.5
rrn5_p4	rrn5	4	CAGCTGAGGATGCCTAACAG	AATGGTGCATTCCAAGGTGT	20	20	55.0	45.0	58.0	58.0	8.4	0.0	0.063182	108952	109071	545-545	0.500000	59.5
rrn5_p5	rrn5	5	GCTGAGGATGCCTAACAGAC	AATGGTGCATTCCAAGGTGT	20	20	55.0	45.0	58.1	58.0	6.4	0.0	0.067265	108952	109071	543-543	0.500000	59.5
rrn5-trnR-ACG_p1	rrn5-trnR-ACG	1	GTCAAGTGGAAGTGCAGTGA	CCACTTCGACAAAAGACCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084648	109072	109327	877-880	1.000000	84.4
rrn5-trnR-ACG_p2	rrn5-trnR-ACG	2	GTCAAGTGGAAGTGCAGTGA	ACTTGCTCTGCTATTCTGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.081971	109072	109327	1023-1026	1.000000	83.8
rrn5-trnR-ACG_p3	rrn5-trnR-ACG	3	TGGTGGTCAAACTGTACTGC	ACTTGCTCTGCTATTCTGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073737	109072	109327	656-656	0.500000	59.4
rrn5-trnR-ACG_p4	rrn5-trnR-ACG	4	TGGTGGTCAAACTGTACTGC	CCACTTCGACAAAAGACCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.076413	109072	109327	510-510	0.500000	59.4
rrn5-trnR-ACG_p5	rrn5-trnR-ACG	5	CAGCTGAGGATGCCTAACAG	ACTTGCTCTGCTATTCTGCC	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.084702	109072	109327	997-997	0.500000	59.3
trnR-ACG_p1	trnR-ACG	1	GTCAAGTGGAAGTGCAGTGA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048772	109328	109401	1460-1463	1.000000	82.7
trnR-ACG_p2	trnR-ACG	2	ACACCTTGGAATGCACCATT	CCGTTGTTCCTCAGTAGCTC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.016341	109328	109401	909-909	0.500000	59.9
trnR-ACG_p3	trnR-ACG	3	TGGTGGTCAAACTGTACTGC	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.040537	109328	109401	1093-1093	0.500000	59.7
trnR-ACG_p4	trnR-ACG	4	ACACCTTGGAATGCACCATT	ACTTGCTCTGCTATTCTGCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.049540	109328	109401	472-472	0.500000	59.6
trnR-ACG_p5	trnR-ACG	5	CAGCTGAGGATGCCTAACAG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	58.0	58.0	8.4	0.0	0.051503	109328	109401	1434-1434	0.500000	59.6
ccsA_p1	ccsA	1	TCCGATTTCCATTTCCAAATTTGT	GCTTTTGCTTATGCCTTGTCC	24	21	33.3	47.6	57.9	58.4	16.7	0.0	5.497468	115892	116866	1097-1099	1.000000	40.4
ccsA_p2	ccsA	2	CCGATTTCCATTTCCAAATTTGT	GCTTTTGCTTATGCCTTGTCC	23	21	34.8	47.6	56.5	58.4	16.7	0.0	5.840115	115892	116866	1096-1098	1.000000	40.4
ccsA_p3	ccsA	3	TCCGATTTCCATTTCCAAATTTGT	TGCTTATGCCTTGTCCTATGT	24	21	33.3	42.9	57.9	57.1	16.7	0.0	6.039808	115892	116866	1092-1094	1.000000	40.4
ccsA_p4	ccsA	4	TTCCGATTTCCATTTCCAAA	GCTTTTGCTTATGCCTTGTCC	20	21	35.0	47.6	53.3	58.4	0.0	0.0	6.053348	115892	116866	1098-1100	1.000000	40.4
ccsA_p5	ccsA	5	TTTCCGATTTCCATTTCCAA	GCTTTTGCTTATGCCTTGTCC	20	21	35.0	47.6	53.3	58.4	0.0	0.0	6.053348	115892	116866	1099-1101	1.000000	40.4
ccsA-ndhD_p1	ccsA-ndhD	1	GGGCTACATAGTTACGGTTCA	TCAGTTGAGAAGGTGGAAGC	21	20	47.6	50.0	57.2	57.4	0.0	0.0	2.357455	116867	117061	285-285	1.000000	64.2
ccsA-ndhD_p2	ccsA-ndhD	2	AGGGCTACATAGTTACGGTTC	TCAGTTGAGAAGGTGGAAGC	21	20	47.6	50.0	56.9	57.4	0.0	0.0	2.642455	116867	117061	286-286	1.000000	61.9
ccsA-ndhD_p3	ccsA-ndhD	3	GGGCTACATAGTTACGGTTCA	ATCAGTTGAGAAGGTGGAAGC	21	21	47.6	47.6	57.2	57.9	0.0	0.0	2.871525	116867	117061	286-286	1.000000	60.1
ccsA-ndhD_p4	ccsA-ndhD	4	GGGCTACATAGTTACGGTTCAT	TCAGTTGAGAAGGTGGAAGC	22	20	45.5	50.0	57.7	57.4	0.0	0.0	2.883258	116867	117061	285-285	1.000000	60.0
ccsA-ndhD_p5	ccsA-ndhD	5	GGGCTACATAGTTACGGTTCA	TGGCATTTACCCAGATTTCGT	21	21	47.6	42.9	57.2	57.9	0.0	0.0	2.886925	116867	117061	316-316	1.000000	59.7
rps15-ycf1_p1	rps15-ycf1	1	CAGACCCCCTGTTTTCTTCT	AGCTTGTATGAATCGTTATTGGT	20	23	50.0	34.8	57.0	56.3	0.0	0.0	5.626084	124923	125215	454-455	1.000000	42.3
rps15-ycf1_p2	rps15-ycf1	2	CAGACCCCCTGTTTTCTTCTT	AGCTTGTATGAATCGTTATTGGT	21	23	47.6	34.8	57.8	56.3	0.0	0.0	5.896316	124923	125215	454-455	1.000000	42.3
rps15-ycf1_p3	rps15-ycf1	3	CAGACCCCCTGTTTTCTTCT	AGCTTGTATGAATCGTTATTGGTT	20	24	50.0	33.3	57.0	57.0	0.0	0.0	5.961896	124923	125215	454-455	1.000000	42.3
rps15-ycf1_p4	rps15-ycf1	4	CAGACCCCCTGTTTTCTTCT	GCTTGTATGAATCGTTATTGGT	20	22	50.0	36.4	57.0	54.9	0.0	0.0	6.056979	124923	125215	453-454	1.000000	42.3
rps15-ycf1_p5	rps15-ycf1	5	TGAAATTCAACAGACCCCCT	AGCTTGTATGAATCGTTATTGGT	20	23	45.0	34.8	56.6	56.3	0.0	0.0	6.036136	124923	125215	464-465	1.000000	42.2
trnR-ACG_p1	trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	GTCAAGTGGAAGTGCAGTGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048772	131926	131999	1460-1463	1.000000	82.7
trnR-ACG_p2	trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	ACACCTTGGAATGCACCATT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016341	131926	131999	909-909	0.500000	59.9
trnR-ACG_p3	trnR-ACG	3	CCGTTGTTCCTCAGTAGCTC	TGGTGGTCAAACTGTACTGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.040537	131926	131999	1093-1093	0.500000	59.7
trnR-ACG_p4	trnR-ACG	4	ACTTGCTCTGCTATTCTGCC	ACACCTTGGAATGCACCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.049540	131926	131999	472-472	0.500000	59.6
trnR-ACG_p5	trnR-ACG	5	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGATGCCTAACAG	20	20	55.0	55.0	58.0	58.0	0.0	8.4	0.051503	131926	131999	1434-1434	0.500000	59.6
trnR-ACG-rrn5_p1	trnR-ACG-rrn5	1	CCACTTCGACAAAAGACCCA	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084648	132000	132255	877-880	1.000000	84.4
trnR-ACG-rrn5_p2	trnR-ACG-rrn5	2	ACTTGCTCTGCTATTCTGCC	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.081971	132000	132255	1023-1026	1.000000	83.8
trnR-ACG-rrn5_p3	trnR-ACG-rrn5	3	ACTTGCTCTGCTATTCTGCC	TGGTGGTCAAACTGTACTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073737	132000	132255	656-656	0.500000	59.4
trnR-ACG-rrn5_p4	trnR-ACG-rrn5	4	CCACTTCGACAAAAGACCCA	TGGTGGTCAAACTGTACTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.076413	132000	132255	510-510	0.500000	59.4
trnR-ACG-rrn5_p5	trnR-ACG-rrn5	5	ACTTGCTCTGCTATTCTGCC	CAGCTGAGGATGCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.084702	132000	132255	997-997	0.500000	59.3
rrn5_p1	rrn5	1	AATGGTGCATTCCAAGGTGT	ACCTGGGGCTGTAGTATGTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.047165	132256	132375	984-984	0.500000	59.6
rrn5_p2	rrn5	2	AATGGTGCATTCCAAGGTGT	TGGGGCTGTAGTATGTTCCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054300	132256	132375	981-981	0.500000	59.6
rrn5_p3	rrn5	3	AATGGTGCATTCCAAGGTGT	GTCAAGTGGAAGTGCAGTGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.060451	132256	132375	571-571	0.500000	59.5
rrn5_p4	rrn5	4	AATGGTGCATTCCAAGGTGT	CAGCTGAGGATGCCTAACAG	20	20	45.0	55.0	58.0	58.0	0.0	8.4	0.063182	132256	132375	545-545	0.500000	59.5
rrn5_p5	rrn5	5	AATGGTGCATTCCAAGGTGT	GCTGAGGATGCCTAACAGAC	20	20	45.0	55.0	58.0	58.1	0.0	6.4	0.067265	132256	132375	543-543	0.500000	59.5
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	ACGCCTAGGACACCAGAATA	CCTAACAGACCGATAGACTTGA	20	22	50.0	45.5	57.8	56.8	14.8	0.0	3.403490	132376	132631	300-300	1.000000	56.2
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TACGCCTAGGACACCAGAAT	CCTAACAGACCGATAGACTTGA	20	22	50.0	45.5	57.8	56.8	14.8	0.0	3.403490	132376	132631	301-301	1.000000	56.2
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	GTTCCTCTACGCCTAGGACA	CCTAACAGACCGATAGACTTGA	20	22	55.0	45.5	58.2	56.8	14.8	0.0	3.488731	132376	132631	308-308	1.000000	55.5
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	TTCCTCTACGCCTAGGACAC	CCTAACAGACCGATAGACTTGA	20	22	55.0	45.5	58.2	56.8	14.8	0.0	3.488731	132376	132631	307-307	1.000000	55.5
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	ACGCCTAGGACACCAGAATA	ACAGACCGATAGACTTGAAC	20	20	50.0	45.0	57.8	54.5	14.8	0.0	3.608241	132376	132631	296-296	1.000000	54.6