primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8615	9350	791-810	1.000000	53.8
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8615	9350	794-813	1.000000	52.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8615	9350	793-812	1.000000	52.7
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8615	9350	792-811	1.000000	51.9
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ATTAGCAATCCGCCGCTTTA	CAAATTGAAAGACCCCTTAACCA	20	23	45.0	39.1	58.0	57.2	0.0	0.0	3.838033	8615	9350	831-850	1.000000	51.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.476375	27034	28309	1405-1444	1.000000	78.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCGTCAAGCCCTGATCAATA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.702379	27034	28309	1396-1435	1.000000	76.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.746530	27034	28309	1325-1364	1.000000	76.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	58.4	0.0	14.6	0.749738	27034	28309	1387-1426	1.000000	76.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGTCAAGCCCTGATCAATA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.4	58.4	0.0	14.6	0.975742	27034	28309	1378-1417	1.000000	74.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCTGCGATCTAGGCA	AACGTACGGAAAGAGAGGGA	22	20	45.5	50.0	58.1	58.1	0.0	21.1	2.154524	46220	47142	1031-1074	1.000000	65.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCTGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	22	20	45.5	50.0	58.1	58.1	0.0	0.0	2.164027	46220	47142	1005-1048	1.000000	65.7
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCATTTATCTGCGATCTAGGCA	ACGTACGGAAAGAGAGGGAT	22	20	45.5	50.0	58.1	57.9	0.0	21.1	2.210503	46220	47142	1030-1073	1.000000	65.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CCATTTATCTGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	22	20	45.5	50.0	58.1	58.2	0.0	0.0	2.221013	46220	47142	1026-1069	1.000000	65.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCATTTATCTGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	22	20	45.5	45.0	58.1	57.8	0.0	5.6	2.262328	46220	47142	976-1019	1.000000	65.0
accD-psaI_p1	accD-psaI	1	AGCTACACGGTTTCTTTCCC	GCCATTGAAATTGCCGGAAA	20	20	50.0	45.0	57.8	57.9	0.0	5.0	0.272284	60555	61241	802-850	1.000000	80.4
accD-psaI_p2	accD-psaI	2	GCTACACGGTTTCTTTCCCT	GCCATTGAAATTGCCGGAAA	20	20	50.0	45.0	57.8	57.9	0.0	5.0	0.272284	60555	61241	801-849	1.000000	80.4
accD-psaI_p3	accD-psaI	3	AGCTACACGGTTTCTTTCCC	GAAGCCATTGAAATTGCCGG	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.536552	60555	61241	805-853	1.000000	78.3
accD-psaI_p4	accD-psaI	4	GCTACACGGTTTCTTTCCCT	GAAGCCATTGAAATTGCCGG	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.536552	60555	61241	804-852	1.000000	78.3
accD-psaI_p5	accD-psaI	5	GCTACACGGTTTCTTTCCCT	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	57.8	57.5	0.0	8.9	0.632727	60555	61241	789-837	1.000000	77.6
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TGTCGTGTCATTGATTTCTGC	20	21	50.0	42.9	58.0	57.2	7.6	0.0	1.820923	88700	88767	126-126	1.000000	71.1
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GTCGTGTCATTGATTTCTGCT	20	21	50.0	42.9	58.0	56.9	7.6	0.0	2.100021	88700	88767	125-125	1.000000	69.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	AGTTGGGCGCTTTAACCATT	TGTCGTGTCATTGATTTCTGC	20	21	45.0	42.9	58.4	57.2	7.6	0.0	2.183858	88700	88767	127-127	1.000000	68.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	TTGGGCGCTTTAACCATTCA	TGTCGTGTCATTGATTTCTGC	20	21	45.0	42.9	58.4	57.2	7.6	0.0	2.189730	88700	88767	125-125	1.000000	68.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	ATGTCGTGTCATTGATTTCTGC	20	22	50.0	40.9	58.0	57.7	7.6	0.0	2.355934	88700	88767	127-127	1.000000	66.8
ycf2_p1	ycf2	1	CTTTGACCCCCGAATTTGGA	CGATTTCTTCCGGAAGCAGA	20	20	50.0	50.0	58.1	58.0	13.8	34.9	0.092273	89451	89570	1286-1286	0.666667	65.9
ycf2_p2	ycf2	2	CTTTGACCCCCGAATTTGGA	TCGATTTCTTCCGGAAGCAG	20	20	50.0	50.0	58.1	58.0	13.8	34.9	0.092273	89451	89570	1287-1287	0.666667	65.9
ycf2_p3	ycf2	3	TCTTTGACCCCCGAATTTGG	CGATTTCTTCCGGAAGCAGA	20	20	50.0	50.0	58.1	58.0	4.5	34.9	0.092273	89451	89570	1287-1287	0.666667	65.9
ycf2_p4	ycf2	4	TTTGACCCCCGAATTTGGAG	CGATTTCTTCCGGAAGCAGA	20	20	50.0	50.0	58.1	58.0	13.8	34.9	0.092273	89451	89570	1285-1285	0.666667	65.9
ycf2_p5	ycf2	5	TTTGACCCCCGAATTTGGAG	TCGATTTCTTCCGGAAGCAG	20	20	50.0	50.0	58.1	58.0	13.8	34.9	0.092273	89451	89570	1286-1286	0.666667	65.9
rps7-rps12_p1	rps7-rps12	1	TTCTTCTGCAGTACCTCGAC	TCTTTCGAGATCCACCCTACA	20	21	50.0	47.6	57.0	57.9	14.7	0.0	2.107606	99998	100050	126-126	1.000000	65.7
rps7-rps12_p2	rps7-rps12	2	TCTTCTGCAGTACCTCGACG	TCTTTCGAGATCCACCCTACA	20	21	55.0	47.6	59.2	57.9	14.7	0.0	2.292795	99998	100050	125-125	1.000000	64.2
rps7-rps12_p3	rps7-rps12	3	TTTCTTCTGCAGTACCTCGAC	TCTTTCGAGATCCACCCTACA	21	21	47.6	47.6	57.7	57.9	14.7	0.0	2.395252	99998	100050	127-127	1.000000	63.4
rps7-rps12_p4	rps7-rps12	4	CTTCTGCAGTACCTCGACG	TCTTTCGAGATCCACCCTACA	19	21	57.9	47.6	57.7	57.9	14.7	0.0	2.410251	99998	100050	124-124	1.000000	63.3
rps7-rps12_p5	rps7-rps12	5	TTCTGCAGTACCTCGACGT	TCTTTCGAGATCCACCCTACA	19	21	52.6	47.6	58.4	57.9	14.7	0.0	2.458391	99998	100050	123-123	1.000000	62.9
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TACTCCGACAGCATCTAGGG	CCTCAGCCTACGGGGTATTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.022710	100851	102747	2566-2584	1.000000	82.8
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	AGCGATTCACATGGCATCAT	CCTCAGCCTACGGGGTATTA	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.028314	100851	102747	2648-2667	1.000000	82.7
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TCTACAACGCACTAGAACGC	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.032232	100851	102747	2602-2621	1.000000	82.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TACTCCGACAGCATCTAGGG	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.026092	100851	102747	2750-2758	0.666667	66.5
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	AGCGATTCACATGGCATCAT	CATTGCGGAAAATTCCCCAC	20	20	45.0	50.0	58.0	58.0	0.0	6.6	0.031696	100851	102747	2833-2841	0.666667	66.4
rrn16_p1	rrn16	1	GGCTCCAAGTTGTTCAGGAA	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.034771	103047	104537	1948-1962	1.000000	83.6
rrn16_p2	rrn16	2	GGCTCCAAGTTGTTCAGGAA	TCGCTTCTTTTTGGGTGTGA	20	20	50.0	45.0	58.0	58.0	1.4	0.0	0.064305	103047	104537	1938-1952	1.000000	83.4
rrn16_p3	rrn16	3	GGCTCCAAGTTGTTCAGGAA	TGAATCGCCCCATCTATCCT	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.048771	103047	104537	2378-2396	1.000000	83.3
rrn16_p4	rrn16	4	TAGTGGCGTTGAGTTTCTCG	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.076353	103047	104537	1928-1934	1.000000	83.3
rrn16_p5	rrn16	5	TAGTGGCGTTGAGTTTCTCG	TGAATCGCCCCATCTATCCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.090353	103047	104537	2358-2368	1.000000	83.0
trnA-UGC_p1	trnA-UGC	1	GCATCTGACTCCTTCATGCA	TATCGGCTAAGTTCACGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.521056	105908	106780	913-930	1.000000	81.1
trnA-UGC_p2	trnA-UGC	2	TCTGACTCCTTCATGCATGC	TATCGGCTAAGTTCACGAGC	20	20	50.0	50.0	58.0	57.5	22.2	0.0	0.521056	105908	106780	910-927	1.000000	81.1
trnA-UGC_p3	trnA-UGC	3	TGACTCCTTCATGCATGCTC	TATCGGCTAAGTTCACGAGC	20	20	50.0	50.0	58.0	57.5	22.2	0.0	0.521056	105908	106780	908-925	1.000000	81.1
trnA-UGC_p4	trnA-UGC	4	TGATTTACTTCACGGGCGAG	TATCGGCTAAGTTCACGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.491534	105908	106780	988-1005	1.000000	81.0
trnA-UGC_p5	trnA-UGC	5	GCGCCAGGGAAAAGAATAGA	TATCGGCTAAGTTCACGAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.525338	105908	106780	937-954	1.000000	80.9
rrn23_p1	rrn23	1	ACCTTAGGATGGCAGCCTTA	AAACTTCTGTCGCTCCATCC	20	20	50.0	50.0	58.1	57.9	31.4	0.0	0.207289	106931	109739	2933-2935	1.000000	82.1
rrn23_p2	rrn23	2	ACCTTAGGATGGCAGCCTTA	GGGGAAGTCGGAATAGGAGA	20	20	50.0	55.0	58.1	57.9	31.4	0.0	0.190313	106931	109739	2864-2864	0.666667	65.2
rrn23_p3	rrn23	3	GTTCGAGTCCGCTTATCTCC	GGGGAAGTCGGAATAGGAGA	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.206191	106931	109739	3000-3000	0.666667	65.1
rrn23_p4	rrn23	4	GGATGGCAGCCTTAAAGTGA	GGGGAAGTCGGAATAGGAGA	20	20	50.0	55.0	57.9	57.9	16.0	0.0	0.212399	106931	109739	2858-2858	0.666667	65.0
rrn23_p5	rrn23	5	TCCTTCCATTTAGCAGCACC	GGGGAAGTCGGAATAGGAGA	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.212399	106931	109739	2881-2881	0.666667	65.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGACAGCGGTAAAAGCTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	5.7	0.0	0.032171	116300	117285	1083-1114	1.000000	82.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTGGACAGCGGTAAAAGCTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.032171	116300	117285	1084-1115	1.000000	82.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGGACAGCGGTAAAAGCTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.0	58.0	5.7	0.0	0.033541	116300	117285	1084-1115	1.000000	82.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGGACAGCGGTAAAAGCTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.033541	116300	117285	1085-1116	1.000000	82.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGGACAGCGGTAAAAGCTTT	TTTAGAAGATGCCGCCACTC	20	20	45.0	50.0	58.0	58.0	5.7	0.0	0.048516	116300	117285	1143-1174	1.000000	82.1
rrn23_p1	rrn23	1	GCTCTGGGGAAGTCGGAAT	ACCTTAGGATGGCAGCCTTA	19	20	57.9	50.0	59.1	58.1	0.0	31.4	2.198202	134620	137428	2869-2870	1.000000	66.2
rrn23_p2	rrn23	2	GCTCTGGGGAAGTCGGAAT	GGATGGCAGCCTTAAAGTGA	19	20	57.9	50.0	59.1	57.9	0.0	16.0	2.220288	134620	137428	2863-2864	1.000000	66.0
rrn23_p3	rrn23	3	GCTCTGGGGAAGTCGGAAT	AGGATGGCAGCCTTAAAGTG	19	20	57.9	50.0	59.1	57.9	0.0	20.7	2.226306	134620	137428	2864-2865	1.000000	66.0
rrn23_p4	rrn23	4	GCTCTGGGGAAGTCGGAA	ACCTTAGGATGGCAGCCTTA	18	20	61.1	50.0	58.6	58.1	0.0	31.4	2.717843	134620	137428	2869-2870	1.000000	62.0
rrn23_p5	rrn23	5	GCTCTGGGGAAGTCGGAA	GGATGGCAGCCTTAAAGTGA	18	20	61.1	50.0	58.6	57.9	0.0	16.0	2.739929	134620	137428	2863-2864	1.000000	61.9
trnI-GAU_p1	trnI-GAU	1	CCTCTACGTTCTTGACAGCC	TGAGCTAACCCCAAAAACCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006268	138522	139532	1845-1845	0.666667	66.6
trnI-GAU_p2	trnI-GAU	2	CTCTACGTTCTTGACAGCCC	TGAGCTAACCCCAAAAACCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006268	138522	139532	1844-1844	0.666667	66.6
trnI-GAU_p3	trnI-GAU	3	CCTCTACGTTCTTGACAGCC	AAAGAAGCGAGCTACGTCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018359	138522	139532	1519-1519	0.666667	66.5
trnI-GAU_p4	trnI-GAU	4	CTCTACGTTCTTGACAGCCC	AAAGAAGCGAGCTACGTCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018359	138522	139532	1518-1518	0.666667	66.5
trnI-GAU_p5	trnI-GAU	5	TAAGGATTCTTGCGGTTCCG	TGAGCTAACCCCAAAAACCC	20	20	50.0	50.0	58.0	58.0	6.9	0.0	0.022479	138522	139532	2312-2312	0.666667	66.5
rps12_p1	rps12	1	CCCTTCGCTCGATGAGAAAA	GCTCTATTTGCCTCTGCCAT	20	20	50.0	50.0	58.0	58.0	6.7	0.0	0.031691	143515	144314	1876-1884	1.000000	82.9
rps12_p2	rps12	2	CCCTTCGCTCGATGAGAAAA	GCAGCATCCACTAATTCGGA	20	20	50.0	50.0	58.0	58.0	6.7	0.0	0.056586	143515	144314	1810-1818	1.000000	82.8
rps12_p3	rps12	3	CCCTTCGCTCGATGAGAAAA	AAGCTCTATTTGCCTCTGCC	20	20	50.0	50.0	58.0	58.0	6.7	0.0	0.060033	143515	144314	1878-1886	1.000000	82.6
rps12_p4	rps12	4	CCCTTCGCTCGATGAGAAAA	CATATTTCGACCCGGACGTT	20	20	50.0	50.0	58.0	58.1	6.7	0.7	0.079578	143515	144314	1775-1783	1.000000	82.6
rps12_p5	rps12	5	TCGCTCGATGAGAAAATGGG	GCTCTATTTGCCTCTGCCAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081571	143515	144314	1872-1880	1.000000	82.5
trnL-CAA_p1	trnL-CAA	1	AGCAATTGGATGTCCGACTC	CCTTTCTCTTCGTCTCAGGC	20	20	50.0	55.0	58.0	58.0	7.7	0.0	0.033035	147953	148033	500-500	1.000000	83.4
trnL-CAA_p2	trnL-CAA	2	AGCAATTGGATGTCCGACTC	CTTTCTCTTCGTCTCAGGCC	20	20	50.0	55.0	58.0	58.0	7.7	0.0	0.033035	147953	148033	499-499	1.000000	83.3
trnL-CAA_p3	trnL-CAA	3	TGCAATTGCTCAGGATACCC	CCTTTCTCTTCGTCTCAGGC	20	20	50.0	55.0	57.9	58.0	29.4	0.0	0.060334	147953	148033	959-959	0.666667	66.2
trnL-CAA_p4	trnL-CAA	4	TGCAATTGCTCAGGATACCC	CTTTCTCTTCGTCTCAGGCC	20	20	50.0	55.0	57.9	58.0	29.4	0.0	0.060334	147953	148033	958-958	0.666667	66.2
trnL-CAA_p5	trnL-CAA	5	AGCAATTGGATGTCCGACTC	TGGACCTTAGCTCCATGGAA	20	20	50.0	50.0	58.0	58.0	7.7	17.0	0.064424	147953	148033	797-797	0.666667	66.2
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CCAAATTCGGGGGTCAAAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	155598	155665	285-289	1.000000	85.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.036329	155598	155665	812-812	0.666667	66.4
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	AATCATGGTCCTTGCGGATC	CCACAATGACGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	6.3	0.049892	155598	155665	1435-1435	0.666667	66.3
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CTCCAAATTCGGGGGTCAAA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	155598	155665	291-291	0.666667	65.9
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	TCCAAATTCGGGGGTCAAAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	155598	155665	290-290	0.666667	65.9