primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	5794	6540	80-976	1.000000	86.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	5794	6540	80-918	1.000000	86.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	5794	6540	80-959	1.000000	86.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5794	6540	80-901	1.000000	86.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	58.2	58.0	7.8	0.0	0.217997	5794	6540	80-977	1.000000	85.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9248	9410	80-266	1.000000	70.6
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9248	9410	80-269	1.000000	70.3
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9248	9410	80-272	1.000000	69.8
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9248	9410	80-275	1.000000	69.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9248	9410	80-270	1.000000	68.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	AACGGGGCTTCACAATCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.031752	26303	27403	80-1846	1.000000	84.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	ACGGGGCTTCACAATCTTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.031752	26303	27403	80-1845	1.000000	84.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCGCCTACACAAGCAAATTG	AACGGGGCTTCACAATCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023370	26303	27403	80-2038	1.000000	84.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCGCCTACACAAGCAAATTG	ACGGGGCTTCACAATCTTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023370	26303	27403	80-2037	1.000000	84.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGCCTACACAAGCAAATTG	GAAGGGAATCGCGAAGTCTT	20	20	50.0	50.0	58.0	58.0	0.0	15.2	0.030286	26303	27403	80-2112	1.000000	83.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	27475	28308	80-949	1.000000	85.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	27475	28308	80-947	1.000000	85.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	27475	28308	80-950	1.000000	84.8
trnC-GCA-petN_p4	trnC-GCA-petN	4	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	27475	28308	80-1001	1.000000	84.7
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	27475	28308	80-999	1.000000	84.7
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	28399	28906	80-595	1.000000	83.6
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	28399	28906	80-593	1.000000	83.6
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.516362	28399	28906	80-592	1.000000	83.1
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	28399	28906	80-592	1.000000	82.2
petN-psbM_p5	petN-psbM	5	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	28399	28906	80-594	1.000000	82.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	29935	30504	80-727	1.000000	80.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	29935	30504	80-706	1.000000	80.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	29935	30504	80-703	1.000000	80.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.3	58.2	0.0	0.0	0.414292	29935	30504	80-724	1.000000	79.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.546195	29935	30504	80-729	1.000000	78.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAG	20	20	50.0	50.0	57.9	58.1	4.1	15.7	0.211199	40383	41131	80-849	1.000000	85.4
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	40383	41131	80-856	1.000000	84.8
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	40383	41131	80-860	1.000000	84.6
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.405330	40383	41131	80-855	1.000000	83.8
psaA-ycf3_p5	psaA-ycf3	5	TTTGGCCCATTCCTCGAAAG	TCACGAGGCGTTTCGAATAG	20	20	50.0	50.0	58.5	58.1	0.0	15.7	0.551132	40383	41131	80-853	1.000000	82.6
rpl20-rps12_p1	rpl20-rps12	1	CGGGCTATATATCCGCGTTT	CGAGGAACATGTACTAGGGTG	20	21	50.0	52.4	57.7	57.6	4.9	0.0	1.727258	66373	67105	80-799	1.000000	70.4
rpl20-rps12_p2	rpl20-rps12	2	TCCGCGTTTAATTCTGGTCA	CGAGGAACATGTACTAGGGTG	20	21	45.0	52.4	57.5	57.6	4.9	0.0	1.897357	66373	67105	80-788	1.000000	69.1
rpl20-rps12_p3	rpl20-rps12	3	TTTTTGTTCTACGCCTCCGG	CGAGGAACATGTACTAGGGTG	20	21	50.0	52.4	58.5	57.6	0.0	0.0	1.923215	66373	67105	80-816	1.000000	68.7
rpl20-rps12_p4	rpl20-rps12	4	CGGGCTATATATCCGCGTTT	CGAGGAACATGTACTAGGGT	20	20	50.0	50.0	57.7	56.1	4.9	0.0	2.222461	66373	67105	80-799	1.000000	66.4
rpl20-rps12_p5	rpl20-rps12	5	CCCCGAAAACTTGATGCAAA	CGAGGAACATGTACTAGGGTG	20	21	45.0	52.4	57.2	57.6	0.0	0.0	2.248736	66373	67105	80-844	1.000000	66.1
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	21	20	47.6	55.0	57.6	58.4	0.0	0.0	1.824342	71460	71532	80-133	1.000000	70.4
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	21	20	47.6	55.0	57.6	57.7	0.0	0.0	1.680523	71460	71532	80-181	1.000000	70.3
psbT-psbN_p3	psbT-psbN	3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	71460	71532	80-189	1.000000	69.4
psbT-psbN_p4	psbT-psbN	4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	71460	71532	80-192	1.000000	69.3
psbT-psbN_p5	psbT-psbN	5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	21	20	47.6	55.0	57.6	57.5	0.0	4.1	1.945894	71460	71532	80-182	1.000000	68.2
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCCGGTGA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.064600	106778	109006	80-2523	1.000000	82.8
ndhF_p2	ndhF	2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCCGGTGAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.064600	106778	109006	80-2522	1.000000	82.8
ndhF_p3	ndhF	3	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCCGGTGAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.067186	106778	109006	80-2514	1.000000	82.7
ndhF_p4	ndhF	4	TGACAAATTCTACGGCTCGG	AAAGAGGTAAGGTCCGGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024390	106778	109006	80-2716	0.800000	74.4
ndhF_p5	ndhF	5	TGACAAATTCTACGGCTCGG	AAGAGGTAAGGTCCGGTGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024390	106778	109006	80-2715	0.800000	74.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCACCGGACCTTACCTCTTT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.027638	109626	110127	80-1400	1.000000	87.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTCACCGGACCTTACCTCTT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.027638	109626	110127	80-1401	1.000000	87.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCACCGGACCTTACCTCTTT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.029008	109626	110127	80-1401	1.000000	87.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTCACCGGACCTTACCTCTT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.029008	109626	110127	80-1402	1.000000	87.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCACCGGACCTTACCTCTTT	TGATCCACATAAAAGGGCCG	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.071142	109626	110127	80-2000	1.000000	86.4
ccsA-ndhD_p1	ccsA-ndhD	1	CGGCCCTTTTATGTGGATCA	GATATGGGACTCAACGGAGC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.207677	111267	111447	80-1243	1.000000	82.2
ccsA-ndhD_p2	ccsA-ndhD	2	CGGCCCTTTTATGTGGATCA	CTCAACGGAGCCCTTTTACA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.230246	111267	111447	80-1234	1.000000	82.1
ccsA-ndhD_p3	ccsA-ndhD	3	CGGCCCTTTTATGTGGATCA	ACTCAACGGAGCCCTTTTAC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.231766	111267	111447	80-1235	1.000000	82.1
ccsA-ndhD_p4	ccsA-ndhD	4	TTGGCCCGAGTGCTATTTTT	GATATGGGACTCAACGGAGC	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.238471	111267	111447	80-1381	1.000000	81.9
ccsA-ndhD_p5	ccsA-ndhD	5	CGGCCCTTTTATGTGGATCA	TCTCTCGGTCAACGCAATTT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.215152	111267	111447	80-1333	0.800000	73.5
ndhD_p1	ndhD	1	CGGCCCTTTTATGTGGATCA	AGGACTGCGTTGGTTGTAAG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.172381	111448	112968	80-2447	1.000000	82.0
ndhD_p2	ndhD	2	CGGCCCTTTTATGTGGATCA	CCCTGGGATGGATGTAAAGC	20	20	50.0	55.0	57.9	58.0	0.0	7.0	0.064452	111448	112968	80-2494	0.700000	70.3
ndhD_p3	ndhD	3	AGTTGGGACCCAAAGGAAAC	CCCTGGGATGGATGTAAAGC	20	20	50.0	55.0	57.9	58.0	27.9	7.0	0.095258	111448	112968	80-2174	0.700000	70.0
ndhD_p4	ndhD	4	GTTGGGACCCAAAGGAAACT	CCCTGGGATGGATGTAAAGC	20	20	50.0	55.0	57.9	58.0	27.9	7.0	0.095258	111448	112968	80-2173	0.700000	70.0
ndhD_p5	ndhD	5	GAAGCGTGGGGATCCTATTG	CCCTGGGATGGATGTAAAGC	20	20	55.0	55.0	58.1	58.0	12.6	7.0	0.128882	111448	112968	80-2195	0.700000	69.8
ndhD-psaC_p1	ndhD-psaC	1	GCAAGGAAAATGGTTCGTGG	AAGAGATGTGAATCCGCCTG	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.386929	112969	113071	80-233	1.000000	82.4
ndhD-psaC_p2	ndhD-psaC	2	GCAAGGAAAATGGTTCGTGG	GAGATGTGAATCCGCCTGTC	20	20	50.0	55.0	57.9	58.4	0.0	0.0	0.488218	112969	113071	80-231	1.000000	81.7
ndhD-psaC_p3	ndhD-psaC	3	GCAAGGAAAATGGTTCGTGG	ATGGCATGAAACAACTCGCA	20	20	50.0	45.0	57.9	58.8	0.0	0.0	0.817924	112969	113071	80-180	1.000000	80.5
ndhD-psaC_p4	ndhD-psaC	4	GCAAGGAAAATGGTTCGTGG	AGAGATGTGAATCCGCCTGT	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.865860	112969	113071	80-232	1.000000	78.6
ndhD-psaC_p5	ndhD-psaC	5	GCAAGGAAAATGGTTCGTGG	TGTAAGAGATGTGAATCCGCC	20	21	50.0	47.6	57.9	57.8	0.0	0.0	1.244016	112969	113071	80-236	1.000000	75.5
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAACCGTCCAAAGATGAA	GAGGCCGAATCCACTTTGAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.274497	114615	114989	80-1010	1.000000	83.1
ndhG-ndhI_p2	ndhG-ndhI	2	ATGATCCCATACCAGGACGT	GAGGCCGAATCCACTTTGAA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.281937	114615	114989	80-1093	1.000000	82.6
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAACCGTCCAAAGATGAA	CTGCCAGGTACATTGGTCAA	20	20	50.0	50.0	58.1	57.8	0.0	14.8	0.294540	114615	114989	80-1118	1.000000	82.6
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAACCGTCCAAAGATGAA	TGCCAGGTACATTGGTCAAG	20	20	50.0	50.0	58.1	57.8	0.0	14.8	0.294540	114615	114989	80-1117	1.000000	82.6
ndhG-ndhI_p5	ndhG-ndhI	5	ATGATCCCATACCAGGACGT	TGCCAGGTACATTGGTCAAG	20	20	50.0	50.0	57.9	57.8	0.0	14.8	0.301980	114615	114989	80-1200	1.000000	82.2
rps15-ycf1_p1	rps15-ycf1	1	ATCCGTTGACGTTTTCCCAA	AGCAGCCAAGTTTCACATGA	20	20	45.0	45.0	58.0	58.0	2.6	0.0	0.044435	119244	119632	80-775	1.000000	85.1
rps15-ycf1_p2	rps15-ycf1	2	CAGCATCCGTTGACGTTTTC	AGCAGCCAAGTTTCACATGA	20	20	50.0	45.0	58.0	58.0	2.6	0.0	0.064055	119244	119632	80-779	1.000000	84.9
rps15-ycf1_p3	rps15-ycf1	3	TGACAAATAGGCCAGCATCC	AGCAGCCAAGTTTCACATGA	20	20	50.0	45.0	57.9	58.0	5.0	0.0	0.071664	119244	119632	80-791	1.000000	84.8
rps15-ycf1_p4	rps15-ycf1	4	ATCCGTTGACGTTTTCCCAA	GCAGCCAAGTTTCACATGAC	20	20	45.0	50.0	58.0	57.9	2.6	0.0	0.086002	119244	119632	80-774	1.000000	84.8
rps15-ycf1_p5	rps15-ycf1	5	AACACCATGCATTGATGGGT	AGCAGCCAAGTTTCACATGA	20	20	45.0	45.0	58.1	58.0	8.9	0.0	0.073150	119244	119632	80-1035	1.000000	83.9
ycf1_p1	ycf1	1	GCCCAAGGTTTTTGGCAATT	CTCGAAACACGGACTCGATT	20	20	45.0	50.0	58.0	58.0	0.0	2.4	0.041604	122969	123088	80-1096	1.000000	85.0
ycf1_p2	ycf1	2	TTTGCCCCTTCTTGAACGAA	CTCGAAACACGGACTCGATT	20	20	45.0	50.0	57.9	58.0	0.0	2.4	0.081508	122969	123088	80-585	0.900000	80.8
ycf1_p3	ycf1	3	AAAAGGGGAGTTCGGTTTCC	CTCGAAACACGGACTCGATT	20	20	50.0	50.0	58.0	58.0	0.0	2.4	0.030337	122969	123088	80-1390	0.800000	75.9
ycf1_p4	ycf1	4	GAACAAAAGGGGAGTTCGGT	CTCGAAACACGGACTCGATT	20	20	50.0	50.0	58.0	58.0	0.0	2.4	0.044402	122969	123088	80-1394	0.800000	75.8
ycf1_p5	ycf1	5	TGAACAAAAGGGGAGTTCGG	CTCGAAACACGGACTCGATT	20	20	50.0	50.0	58.0	58.0	0.0	2.4	0.045799	122969	123088	80-1395	0.800000	75.8