primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbI-trnS-GCU_p1 psbI-trnS-GCU 1 CGTAATCCTGGACGTGAAGA ATTGGGAGAGATGGCTGAGT 20 20 50.0 50.0 57.1 58.1 0.0 0.0 1.042943 8409 8547 223-255 1.000000 77.0 psbI-trnS-GCU_p2 psbI-trnS-GCU 2 CGTAATCCTGGACGTGAAGA CGAGTTATTCGTACCGAGGG 20 20 50.0 55.0 57.1 57.6 0.0 0.0 1.329655 8409 8547 171-203 1.000000 75.1 psbI-trnS-GCU_p3 psbI-trnS-GCU 3 CGTAATCCTGGACGTGAAGAA ATTGGGAGAGATGGCTGAGT 21 20 47.6 50.0 57.8 58.1 0.0 0.0 1.335793 8409 8547 223-255 1.000000 74.7 psbI-trnS-GCU_p4 psbI-trnS-GCU 4 CCTATCCAATGATCCAGGACG ATTGGGAGAGATGGCTGAGT 21 20 52.4 50.0 57.7 58.1 0.0 0.0 1.434740 8409 8547 242-274 1.000000 73.8 psbI-trnS-GCU_p5 psbI-trnS-GCU 5 CGTAATCCTGGACGTGAAGA GATGGCTGAGTGGACGAAAG 20 20 50.0 55.0 57.1 58.6 0.0 0.0 1.569752 8409 8547 214-246 1.000000 72.8 trnS-GCU-trnC-GCA_p1 trnS-GCU-trnC-GCA 1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.2 58.1 0.0 0.0 0.233476 8636 9385 901-902 1.000000 79.6 trnS-GCU-trnC-GCA_p2 trnS-GCU-trnC-GCA 2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 58.2 58.4 0.0 14.6 0.506839 8636 9385 883-884 1.000000 77.5 trnS-GCU-trnC-GCA_p3 trnS-GCU-trnC-GCA 3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 20 20 55.0 50.0 58.6 58.1 0.0 0.0 0.718384 8636 9385 837-838 1.000000 75.9 trnS-GCU-trnC-GCA_p4 trnS-GCU-trnC-GCA 4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 57.3 58.1 0.0 0.0 0.756923 8636 9385 902-903 1.000000 75.5 trnS-GCU-trnC-GCA_p5 trnS-GCU-trnC-GCA 5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 20 20 55.0 50.0 58.6 58.4 0.0 14.6 0.991747 8636 9385 819-820 1.000000 73.7 trnG-UCC-trnfM-CAU_p1 trnG-UCC-trnfM-CAU 1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 57.8 58.0 0.0 4.2 0.208039 36033 36224 260-270 1.000000 83.2 trnG-UCC-trnfM-CAU_p2 trnG-UCC-trnfM-CAU 2 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 57.8 58.0 0.0 0.0 0.195421 36033 36224 296-306 1.000000 82.7 trnG-UCC-trnfM-CAU_p3 trnG-UCC-trnfM-CAU 3 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.277623 36033 36224 254-264 1.000000 82.7 trnG-UCC-trnfM-CAU_p4 trnG-UCC-trnfM-CAU 4 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.348744 36033 36224 253-263 1.000000 82.2 trnG-UCC-trnfM-CAU_p5 trnG-UCC-trnfM-CAU 5 TCTTTGCCAAGGAGAAGACG TTCAGTTCTTCTCTTCGGCG 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.235825 36033 36224 318-328 1.000000 82.1 psaA-D3Q48_pgp065_p1 psaA-D3Q48_pgp065 1 GAGAAATGACCCGGTCTAGC GCTATAGCACTTACTCCTGGT 20 21 55.0 47.6 57.8 56.6 18.5 20.0 2.611864 41373 42098 891-896 1.000000 61.3 psaA-D3Q48_pgp065_p2 psaA-D3Q48_pgp065 2 GAGAAATGACCCGGTCTAGC TGGTTGAAGATCACTAGACGT 20 21 55.0 42.9 57.8 56.4 18.5 0.0 2.811852 41373 42098 849-854 1.000000 59.9 psaA-D3Q48_pgp065_p3 psaA-D3Q48_pgp065 3 TTTTACTTCTGGTTCCGGCG GCTATAGCACTTACTCCTGGT 20 21 50.0 47.6 58.5 56.6 0.0 20.0 2.870181 41373 42098 826-831 1.000000 59.5 psaA-D3Q48_pgp065_p4 psaA-D3Q48_pgp065 4 TTTTACTTCTGGTTCCGGCG TGGTTGAAGATCACTAGACGT 20 21 50.0 42.9 58.5 56.4 0.0 0.0 3.070169 41373 42098 784-789 1.000000 58.0 psaA-D3Q48_pgp065_p5 psaA-D3Q48_pgp065 5 GAGAAATGACCCGGTCTAGC TGGTTGAAGATCACTAGACGTT 20 22 55.0 40.9 57.8 57.1 18.5 0.0 3.093110 41373 42098 849-854 1.000000 57.6 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCCTTTGCTACTCGGAGCTA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.9 57.8 4.6 0.0 0.336606 49656 50415 876-904 1.000000 83.2 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 ACTCGGAGCTAAAAATCCCG AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.7 57.8 4.6 0.0 0.508407 49656 50415 867-895 1.000000 81.9 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 TTCTCCGGTCCTTTGCTACT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.4 57.8 0.0 0.0 0.556367 49656 50415 884-912 1.000000 81.4 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TTTCTCCGGTCCTTTGCTAC AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.664849 49656 50415 885-913 1.000000 80.6 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 TCCTTTGCTACTCGGAGCTA GAAGGTCTACGGTTCGAGTC 20 20 50.0 55.0 57.9 57.5 4.6 0.0 0.680379 49656 50415 875-903 1.000000 80.5 accD-psaI_p1 accD-psaI 1 TCAACTCCACACTTTCTTTCCT AAAGAAGCCATTGCGATTGC 22 20 40.9 45.0 57.5 58.0 0.0 1.6 2.504143 57642 58414 859-887 1.000000 66.3 accD-psaI_p2 accD-psaI 2 TCAACTCCACACTTTCTTTCCT TTGCGATTGCCGGAAATACT 22 20 40.9 45.0 57.5 58.3 0.0 8.0 2.758482 57642 58414 849-877 1.000000 64.2 accD-psaI_p3 accD-psaI 3 TCAACTCCACACTTTCTTTCCT AGGCCTACTAAAGGGACCAA 22 20 40.9 50.0 57.5 57.6 0.0 1.3 2.857975 57642 58414 829-857 1.000000 63.5 accD-psaI_p4 accD-psaI 4 TCAACTCCACACTTTCTTTCCT GGGACCAAAACAGAGGGAAA 22 20 40.9 50.0 57.5 57.6 0.0 0.0 2.878728 57642 58414 817-845 1.000000 63.4 accD-psaI_p5 accD-psaI 5 TCAACTCCACACTTTCTTTCCT AAAGGGACCAAAACAGAGGG 22 20 40.9 50.0 57.5 57.6 0.0 0.0 2.885466 57642 58414 820-848 1.000000 63.3 trnW-CCA-trnP-UGG_p1 trnW-CCA-trnP-UGG 1 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.161494 65610 65771 216-228 1.000000 84.5 trnW-CCA-trnP-UGG_p2 trnW-CCA-trnP-UGG 2 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.162838 65610 65771 217-229 1.000000 84.5 trnW-CCA-trnP-UGG_p3 trnW-CCA-trnP-UGG 3 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.169530 65610 65771 205-217 1.000000 84.5 trnW-CCA-trnP-UGG_p4 trnW-CCA-trnP-UGG 4 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.170864 65610 65771 203-215 1.000000 84.5 trnW-CCA-trnP-UGG_p5 trnW-CCA-trnP-UGG 5 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.170864 65610 65771 206-218 1.000000 84.5 infA-rps8_p1 infA-rps8 1 CGTTGGGAAGCGATTCAGTA CGGGATGGGGGTTGTAATTC 20 20 50.0 55.0 58.0 58.3 16.2 0.0 0.317020 79154 79265 270-270 1.000000 80.7 infA-rps8_p2 infA-rps8 2 CGTTGGGAAGCGATTCAGTA AGAATTTTAGGCGGGATGGG 20 20 50.0 50.0 58.0 57.6 16.2 0.0 0.369555 79154 79265 281-281 1.000000 80.2 infA-rps8_p3 infA-rps8 3 CGTTGGGAAGCGATTCAGTA CGGGGTATAATGACAGACCG 20 20 50.0 55.0 58.0 57.6 16.2 0.0 0.438451 79154 79265 239-239 1.000000 80.2 infA-rps8_p4 infA-rps8 4 CGTTGGGAAGCGATTCAGTA TTCTTTCTACCTCTCGGGGT 20 20 50.0 50.0 58.0 57.4 16.2 0.0 0.622423 79154 79265 253-253 1.000000 78.5 infA-rps8_p5 infA-rps8 5 CGTTGGGAAGCGATTCAGTA GAATTTTAGGCGGGATGGGG 20 20 50.0 55.0 58.0 58.7 16.2 0.0 0.676304 79154 79265 280-280 1.000000 77.7 rpl16-rps3_p1 rpl16-rps3 1 GGAATCTGGTTCTTTTGGGGT CCACTACAAACCATTCGAGC 21 20 47.6 50.0 58.1 56.8 0.0 0.0 2.294076 80753 81905 1289-1319 1.000000 64.5 rpl16-rps3_p2 rpl16-rps3 2 GGAATCTGGTTCTTTTGGGGT CCACTACAAACCATTCGAGCT 21 21 47.6 47.6 58.1 58.3 0.0 0.0 2.410533 80753 81905 1289-1319 1.000000 63.6 rpl16-rps3_p3 rpl16-rps3 3 GGAATCTGGTTCTTTTGGGGT TCCACTACAAACCATTCGAGC 21 21 47.6 47.6 58.1 58.3 0.0 0.0 2.415129 80753 81905 1290-1320 1.000000 63.6 rpl16-rps3_p4 rpl16-rps3 4 CGGAATCTGGTTCTTTTGGG CCACTACAAACCATTCGAGC 20 20 50.0 50.0 56.7 56.8 0.0 0.0 2.487065 80753 81905 1290-1320 1.000000 63.0 rpl16-rps3_p5 rpl16-rps3 5 CGGAATCTGGTTCTTTTGGG CCACTACAAACCATTCGAGCT 20 21 50.0 47.6 56.7 58.3 0.0 0.0 2.603523 80753 81905 1290-1320 1.000000 62.1 ycf1-ndhF_p1 ycf1-ndhF 1 CTAGCACTGGGTAGACCTCA GGGGGTGGACGTATTTCTTC 20 20 55.0 55.0 57.9 58.0 0.0 0.0 0.179501 108341 108425 517-565 1.000000 84.9 ycf1-ndhF_p2 ycf1-ndhF 2 CTAGCACTGGGTAGACCTCA TGTGGGGGTGAGAAAATTGG 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.153639 108341 108425 635-683 1.000000 84.5 ycf1-ndhF_p3 ycf1-ndhF 3 GCCGAGAAGAAGGTATCAGC GGGGGTGGACGTATTTCTTC 20 20 55.0 55.0 58.1 58.0 0.0 0.0 0.174721 108341 108425 601-649 1.000000 84.1 ycf1-ndhF_p4 ycf1-ndhF 4 GCCGAGAAGAAGGTATCAGC TGTGGGGGTGAGAAAATTGG 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.148860 108341 108425 719-767 1.000000 83.9 ycf1-ndhF_p5 ycf1-ndhF 5 TCGTTGTGGTCGGACTCTAT TGTGGGGGTGAGAAAATTGG 20 20 50.0 50.0 58.2 58.0 8.2 0.0 0.177964 108341 108425 823-871 1.000000 83.0 ndhF_p1 ndhF 1 GCCGAGAAGAAGGTATCAGC GAGGTAAGATCCGGTGAATCG 20 21 55.0 52.4 58.1 58.0 0.0 0.0 1.155221 108426 110645 2788-2842 1.000000 72.9 ndhF_p2 ndhF 2 CTAGCACTGGGTAGACCTCA GAGGTAAGATCCGGTGAATCG 20 21 55.0 52.4 57.9 58.0 0.0 0.0 1.160001 108426 110645 2704-2758 1.000000 72.9 ndhF_p3 ndhF 3 TCGTTGTGGTCGGACTCTAT GAGGTAAGATCCGGTGAATCG 20 21 50.0 52.4 58.2 58.0 8.2 0.0 1.184325 108426 110645 2892-2946 1.000000 72.6 ndhF_p4 ndhF 4 ATCAATTCGGTCGTTGTGGT GAGGTAAGATCCGGTGAATCG 20 21 45.0 52.4 57.8 58.0 0.0 0.0 1.193516 108426 110645 2902-2956 1.000000 72.5 ndhF_p5 ndhF 5 AGAAGAAGGAGCCGAGAAGA GAGGTAAGATCCGGTGAATCG 20 21 50.0 52.4 57.8 58.0 0.0 0.0 1.238407 108426 110645 2798-2852 1.000000 72.2 ndhF-rpl32_p1 ndhF-rpl32 1 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 20 24 50.0 29.2 58.0 55.3 17.5 0.0 6.751912 110646 111652 1150-1166 1.000000 44.3 ndhF-rpl32_p2 ndhF-rpl32 2 TGCCGTCGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 58.0 55.3 17.5 0.0 6.763201 110646 111652 1148-1164 1.000000 44.3 ndhF-rpl32_p3 ndhF-rpl32 3 TTGCCGTCGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 58.0 55.3 17.5 0.0 6.763201 110646 111652 1149-1165 1.000000 44.3 ndhF-rpl32_p4 ndhF-rpl32 4 CTTTTTGTTGCCGTCGGAAA TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 57.8 55.3 17.5 0.0 6.915793 110646 111652 1156-1172 1.000000 44.3 ndhF-rpl32_p5 ndhF-rpl32 5 TTTGTTGCCGTCGGAAAAAG TCCAAATATTTTTACGAATACGCT 20 24 45.0 29.2 57.8 55.3 17.5 0.0 6.915793 110646 111652 1153-1169 1.000000 44.3 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GGGAAATCTCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 21 20 52.4 50.0 57.8 58.0 6.3 0.0 1.203301 111818 112587 862-888 1.000000 75.9 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GGGAAATCTCTTTCTACCGGG CAGCGTGTCTACCGATTTCA 21 20 52.4 50.0 57.8 58.0 6.3 0.0 1.204672 111818 112587 863-889 1.000000 75.8 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 GGGAAATCTCTTTCTACCGGG TCCTAAGAGCAGCGTGTCTA 21 20 52.4 50.0 57.8 57.9 6.3 0.0 1.319748 111818 112587 872-898 1.000000 74.9 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GGGAAATCTCTTTCTACCGGG CCTAAGAGCAGCGTGTCTAC 21 20 52.4 55.0 57.8 57.8 6.3 0.0 1.391766 111818 112587 871-897 1.000000 74.3 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GGGAAATCTCTTTCTACCGGG CTAAGAGCAGCGTGTCTACC 21 20 52.4 55.0 57.8 57.8 6.3 0.0 1.391766 111818 112587 870-896 1.000000 74.3 psaC-ndhE_p1 psaC-ndhE 1 ACATCCGTCCCAAGGTATCA CTGAAGCCGCTATTGGACTC 20 20 50.0 55.0 58.1 58.4 0.0 0.0 0.549515 115900 116144 436-439 1.000000 78.0 psaC-ndhE_p2 psaC-ndhE 2 ACATCCGTCCCAAGGTATCA CCGCTATTGGACTCGCTATT 20 20 50.0 50.0 58.1 57.6 0.0 0.0 0.566410 115900 116144 430-433 1.000000 77.9 psaC-ndhE_p3 psaC-ndhE 3 TTTGGCTTTACATCCGTCCC CTGAAGCCGCTATTGGACTC 20 20 50.0 55.0 58.2 58.4 0.0 0.0 0.594665 115900 116144 445-448 1.000000 77.6 psaC-ndhE_p4 psaC-ndhE 4 TTTGGCTTTACATCCGTCCC CCGCTATTGGACTCGCTATT 20 20 50.0 50.0 58.2 57.6 0.0 0.0 0.611560 115900 116144 439-442 1.000000 77.5 psaC-ndhE_p5 psaC-ndhE 5 GGACACATTGAGTACACCCG CTGAAGCCGCTATTGGACTC 20 20 55.0 55.0 58.3 58.4 0.0 0.0 0.701633 115900 116144 389-392 1.000000 77.1 ndhG-ndhI_p1 ndhG-ndhI 1 TCATGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 25 20 36.0 45.0 57.3 58.3 0.0 0.0 5.985812 117194 117566 463-487 1.000000 45.9 ndhG-ndhI_p2 ndhG-ndhI 2 TCATGTATTAGTCCAGGTAAATCCA CAAATTGCTTTGGGTCGGTT 25 20 36.0 45.0 57.3 57.5 0.0 0.0 6.208886 117194 117566 462-486 1.000000 45.9 ndhG-ndhI_p3 ndhG-ndhI 3 CATGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 24 20 37.5 45.0 56.0 58.3 0.0 0.0 6.311708 117194 117566 462-486 1.000000 45.9 ndhG-ndhI_p4 ndhG-ndhI 4 TGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 22 20 36.4 45.0 54.0 58.3 0.0 0.0 6.343140 117194 117566 460-484 1.000000 45.9 ndhG-ndhI_p5 ndhG-ndhI 5 CATGTATTAGTCCAGGTAAATCCA CAAATTGCTTTGGGTCGGTT 24 20 37.5 45.0 56.0 57.5 0.0 0.0 6.534783 117194 117566 461-485 1.000000 45.9 ndhI-ndhA_p1 ndhI-ndhA 1 GCTCGTATTGTTTGTTGGCC TTAGGTGGTCTACGAGCTGC 20 20 50.0 55.0 58.0 59.2 0.0 7.0 1.207078 118068 119697 1776-1802 1.000000 72.0 ndhI-ndhA_p2 ndhI-ndhA 2 GGCTCGTATTGTTTGTTGGC TTAGGTGGTCTACGAGCTGC 20 20 50.0 55.0 58.0 59.2 0.0 7.0 1.207078 118068 119697 1777-1803 1.000000 72.0 ndhI-ndhA_p3 ndhI-ndhA 3 GCTCGTATTGTTTGTTGGCC TTTAGGTGGTCTACGAGCTG 20 20 50.0 50.0 58.0 56.7 0.0 7.0 1.341971 118068 119697 1777-1803 1.000000 71.0 ndhI-ndhA_p4 ndhI-ndhA 4 GGCTCGTATTGTTTGTTGGC TTTAGGTGGTCTACGAGCTG 20 20 50.0 50.0 58.0 56.7 0.0 7.0 1.341971 118068 119697 1778-1804 1.000000 71.0 ndhI-ndhA_p5 ndhI-ndhA 5 CGGCTCGTATTGTTTGTTGG TTAGGTGGTCTACGAGCTGC 20 20 50.0 55.0 57.8 59.2 0.0 7.0 1.425526 118068 119697 1778-1804 1.000000 70.3 rps15-trnN-GUU_p1 rps15-trnN-GUU 1 TGACACTCCCCTTTTTACAGA AATTATGGAACCTGCCCGTC 21 20 42.9 50.0 56.5 57.9 0.0 0.0 2.561309 122031 122150 884-902 1.000000 61.5 rps15-trnN-GUU_p2 rps15-trnN-GUU 2 TGACACTCCCCTTTTTACAGA TATGGAACCTGCCCGTCTAT 21 20 42.9 50.0 56.5 57.9 0.0 0.0 2.605637 122031 122150 881-899 1.000000 61.1 rps15-trnN-GUU_p3 rps15-trnN-GUU 3 TGACACTCCCCTTTTTACAGA TTATGGAACCTGCCCGTCTA 21 20 42.9 50.0 56.5 58.1 0.0 0.0 2.639814 122031 122150 882-900 1.000000 60.9 rps15-trnN-GUU_p4 rps15-trnN-GUU 4 TGACACTCCCCTTTTTACAGA AACCTGCCCGTCTATCTGTA 21 20 42.9 50.0 56.5 57.8 0.0 0.0 2.663094 122031 122150 876-894 1.000000 60.7 rps15-trnN-GUU_p5 rps15-trnN-GUU 5 TGACACTCCCCTTTTTACAGA ACCTGCCCGTCTATCTGTAA 21 20 42.9 50.0 56.5 57.8 0.0 0.0 2.663094 122031 122150 875-893 1.000000 60.7