primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCCTTTCAGGATCAGTCGTG CGATTCAAAGTGTATCGCGC 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.023911 4100 4754 1509-1532 0.750000 77.5 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCCTTTCAGGATCAGTCGTG GATTCAAAGTGTATCGCGCG 20 20 55.0 50.0 58.0 58.0 0.0 26.8 0.023911 4100 4754 1508-1531 0.750000 77.5 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CCTTTCAGGATCAGTCGTGG CGATTCAAAGTGTATCGCGC 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.023911 4100 4754 1508-1531 0.750000 77.5 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CCTTTCAGGATCAGTCGTGG GATTCAAAGTGTATCGCGCG 20 20 55.0 50.0 58.0 58.0 0.0 26.8 0.023911 4100 4754 1507-1530 0.750000 77.5 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 TCCCTCGCTTCATCCAAATG GATTCAAAGTGTATCGCGCG 20 20 50.0 50.0 58.0 58.0 0.0 26.8 0.050861 4100 4754 1456-1479 0.750000 77.5 atpF-atpH_p1 atpF-atpH 1 TGGCCCAAAGAAACGAAAGA GCCTGGTTGTAGCATTAGCA 20 20 45.0 50.0 57.9 58.2 0.0 0.0 0.309857 12398 12788 453-490 1.000000 88.6 atpF-atpH_p2 atpF-atpH 2 GTGGCCCAAAGAAACGAAAG GCCTGGTTGTAGCATTAGCA 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.384008 12398 12788 454-491 1.000000 88.0 atpF-atpH_p3 atpF-atpH 3 ATTAAACCCGAAACTCCCGG GCCTGGTTGTAGCATTAGCA 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.368120 12398 12788 484-521 1.000000 87.7 atpF-atpH_p4 atpF-atpH 4 TGGCCCAAAGAAACGAAAGA TGGCCTGGTTGTAGCATTAG 20 20 45.0 50.0 57.9 57.6 0.0 3.3 0.478418 12398 12788 455-492 1.000000 87.2 atpF-atpH_p5 atpF-atpH 5 AGTGGCCCAAAGAAACGAAA GCCTGGTTGTAGCATTAGCA 20 20 45.0 50.0 58.2 58.2 0.0 0.0 0.479513 12398 12788 455-492 1.000000 87.2 trnC-GCA-petN_p1 trnC-GCA-petN 1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.233476 27836 28525 720-774 1.000000 89.7 trnC-GCA-petN_p2 trnC-GCA-petN 2 CGATATGGCCGAGTGGTAAG AGCCCAAGCGAGACTTACTA 20 20 55.0 50.0 57.9 58.2 0.0 0.0 0.217341 27836 28525 760-814 1.000000 89.4 trnC-GCA-petN_p3 trnC-GCA-petN 3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 58.4 58.2 14.7 0.0 0.506839 27836 28525 738-792 1.000000 87.3 trnC-GCA-petN_p4 trnC-GCA-petN 4 GATATGGCCGAGTGGTAAGG AGCCCAAGCGAGACTTACTA 20 20 55.0 50.0 57.5 58.2 0.0 0.0 0.608714 27836 28525 759-813 1.000000 86.3 trnC-GCA-petN_p5 trnC-GCA-petN 5 CGATATGGCCGAGTGGTAAG GCCCAAGCGAGACTTACTAT 20 20 55.0 50.0 57.9 57.1 0.0 0.0 0.958777 27836 28525 759-813 1.000000 83.5 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 AAATGCAAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 23 20 26.1 50.0 52.7 58.5 0.0 5.4 8.816568 29640 30260 706-791 1.000000 48.9 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 AATGCAAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 23 20 26.1 50.0 52.4 58.5 0.0 5.4 9.034411 29640 30260 705-790 1.000000 48.9 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 AAATGCAAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 24 20 25.0 50.0 53.3 58.5 0.0 5.4 9.197929 29640 30260 706-791 1.000000 48.9 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 AAATGCAAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 23 20 26.1 50.0 52.7 57.0 0.0 5.4 9.336721 29640 30260 707-792 1.000000 48.9 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 AATGCAAGAATATTTACTTCCAT GTTCAATTGGTCAGAGCACC 23 20 26.1 50.0 52.4 57.0 0.0 5.4 9.554565 29640 30260 706-791 1.000000 48.9 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.273369 30717 31461 646-814 0.875000 84.8 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 CCGTTGCCTCCTTGAAAGAG TACCGCTGAGTTAAAAGGGC 20 20 55.0 50.0 58.8 57.9 0.0 0.0 0.943401 30717 31461 647-815 0.875000 79.5 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 AAACCACTAGACGATGGGGG TACCGCTGAGTTAAAAGGGC 20 20 55.0 50.0 59.1 57.9 0.0 0.0 1.193914 30717 31461 618-786 0.875000 77.4 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 CGTTGCCTCCTTGAAAGAGA ACCGCTGAGTTAAAAGGGC 20 19 50.0 52.6 57.8 58.1 0.0 0.0 1.227191 30717 31461 645-813 0.875000 77.2 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 CCGTTGCCTCCTTGAAAGA TACCGCTGAGTTAAAAGGGC 19 20 52.6 50.0 57.7 57.9 0.0 0.0 1.426051 30717 31461 647-815 0.875000 75.6 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 AAGGGCCCTTTTAACTCAGC CCAGCCTCCTAAAGCGAAAT 20 20 50.0 50.0 58.1 58.0 32.5 0.0 0.119392 31534 32219 898-1640 1.000000 89.3 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 AAGGGCCCTTTTAACTCAGC AAACTAGGCGCAAAGAACCA 20 20 50.0 45.0 58.1 58.0 32.5 10.8 0.099609 31534 32219 1272-2014 1.000000 87.7 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 AAGGGCCCTTTTAACTCAGC AACTAGGCGCAAAGAACCAA 20 20 50.0 45.0 58.1 58.0 32.5 10.8 0.099609 31534 32219 1271-2013 1.000000 87.7 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 AAGGGCCCTTTTAACTCAGC ACCAAAACTAGGCGCAAAGA 20 20 50.0 45.0 58.1 58.0 32.5 10.8 0.099609 31534 32219 1276-2018 1.000000 87.7 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 AAGGGCCCTTTTAACTCAGC CACCAAAACTAGGCGCAAAG 20 20 50.0 50.0 58.1 57.9 32.5 10.8 0.125746 31534 32219 1277-2019 1.000000 87.4 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 21 20 47.6 50.0 58.0 58.1 8.2 0.0 1.134847 43865 44594 798-998 1.000000 81.4 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 21 20 47.6 50.0 58.0 58.2 8.2 0.0 1.191833 43865 44594 819-1019 1.000000 80.8 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 21 20 47.6 45.0 58.0 57.8 8.2 0.0 1.233148 43865 44594 769-969 1.000000 80.8 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 21 20 47.6 50.0 58.0 58.3 8.2 0.0 1.301418 43865 44594 771-971 1.000000 80.3 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 21 20 47.6 50.0 58.0 58.4 8.2 0.0 1.427808 43865 44594 799-999 1.000000 79.0 rps4-trnT-UGU_p1 rps4-trnT-UGU 1 GTTAGTCCCGGTAAAGCTCC CTCAGAGGTTAGAGCATCGC 20 20 55.0 55.0 57.7 57.9 0.0 0.0 0.439081 45590 45932 457-508 1.000000 87.4 rps4-trnT-UGU_p2 rps4-trnT-UGU 2 GTTAGTCCCGGTAAAGCTCC ATGGTCATCGGTTCGATTCC 20 20 55.0 50.0 57.7 57.8 0.0 0.0 0.546835 45590 45932 425-476 1.000000 87.0 rps4-trnT-UGU_p3 rps4-trnT-UGU 3 TTTTTGAAACGAGGTCCCCG CTCAGAGGTTAGAGCATCGC 20 20 50.0 55.0 58.7 57.9 0.0 0.0 0.821836 45590 45932 424-475 1.000000 84.8 rps4-trnT-UGU_p4 rps4-trnT-UGU 4 GTTAGTCCCGGTAAAGCTCC CAGAGGTTAGAGCATCGCAT 20 20 55.0 50.0 57.7 57.5 0.0 0.0 0.829177 45590 45932 455-506 1.000000 84.3 rps4-trnT-UGU_p5 rps4-trnT-UGU 5 GTTAGTCCCGGTAAAGCTCC TCAGAGGTTAGAGCATCGCA 20 20 55.0 50.0 57.7 58.5 0.0 0.0 0.832012 45590 45932 456-507 1.000000 84.3 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 46006 46787 786-838 1.000000 90.9 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 46006 46787 818-870 1.000000 90.0 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ATTAAGTCCGCAGCGTCTAC 20 20 55.0 50.0 57.9 57.8 0.0 15.0 0.338047 46006 46787 804-856 1.000000 89.3 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT ATTAAGTCCGCAGCGTCTAC 20 20 50.0 50.0 57.8 57.8 15.5 15.0 0.445800 46006 46787 836-888 1.000000 88.4 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATGCGATGCTCTAACCTCTG ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.5 58.0 0.0 0.0 0.527459 46006 46787 788-840 1.000000 87.8 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 22 20 31.8 55.0 53.9 58.7 0.0 0.0 6.805875 49817 50679 777-1113 1.000000 51.1 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 22 20 31.8 55.0 53.9 58.1 0.0 0.0 6.230058 49817 50679 792-1128 1.000000 51.0 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 ATCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 23 20 30.4 55.0 54.5 58.1 0.0 0.0 6.643914 49817 50679 793-1129 1.000000 51.0 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TCATATTCGTAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 23 20 30.4 55.0 54.5 58.1 0.0 0.0 6.643914 49817 50679 792-1128 1.000000 51.0 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 21 20 33.3 55.0 52.3 58.1 0.0 0.0 6.808247 49817 50679 791-1127 1.000000 51.0 accD-psaI_p1 accD-psaI 1 TGAATCAAAATTCAATAGAGCA AGGGAAAGTCGAAAGTTGTCA 22 21 27.3 42.9 52.1 57.4 0.0 0.0 9.469789 57872 58319 313-681 0.875000 47.0 accD-psaI_p2 accD-psaI 2 TGAATCAAAATTCAATAGAGCAT AGGGAAAGTCGAAAGTTGTCA 23 21 26.1 42.9 52.7 57.4 0.0 0.0 9.805164 57872 58319 313-681 0.875000 47.0 accD-psaI_p3 accD-psaI 3 TGAATCAAAATTCAATAGAGCATT AGGGAAAGTCGAAAGTTGTCA 24 21 25.0 42.9 53.6 57.4 0.0 0.0 9.986796 57872 58319 313-681 0.875000 47.0 accD-psaI_p4 accD-psaI 4 TGAATCAAAATTCAATAGAGCA GGGAAAGTCGAAAGTTGTCA 22 20 27.3 45.0 52.1 55.9 0.0 0.0 10.002143 57872 58319 312-680 0.875000 47.0 accD-psaI_p5 accD-psaI 5 TGAATCAAAATTCAATAGAGCA AGGGAAAGTCGAAAGTTGTCAT 22 22 27.3 40.9 52.1 57.9 0.0 0.0 10.002667 57872 58319 313-681 0.875000 47.0 psaI-ycf4_p1 psaI-ycf4 1 GCAATGGCAATGGCTTCTTT GACCCCGTTATAGGTTGTATCC 20 22 45.0 50.0 57.9 57.7 8.6 0.0 2.366555 58431 58812 483-512 1.000000 71.4 psaI-ycf4_p2 psaI-ycf4 2 GCAATGGCAATGGCTTCTTT ACCCCGTTATAGGTTGTATCCA 20 22 45.0 45.5 57.9 58.3 8.6 0.0 2.447041 58431 58812 482-511 1.000000 70.8 psaI-ycf4_p3 psaI-ycf4 3 GCAATGGCAATGGCTTCTTT CCCCGTTATAGGTTGTATCCA 20 21 45.0 47.6 57.9 56.6 8.6 0.0 2.513345 58431 58812 481-510 1.000000 70.3 psaI-ycf4_p4 psaI-ycf4 4 GCAATGGCAATGGCTTCTTT ACCCCGTTATAGGTTGTATCC 20 21 45.0 47.6 57.9 56.6 8.6 0.0 2.515594 58431 58812 482-511 1.000000 70.2 psaI-ycf4_p5 psaI-ycf4 5 GCAATGGCAATGGCTTCTTT CCCCGTTATAGGTTGTATCCAT 20 22 45.0 45.5 57.9 57.1 8.6 0.0 3.006582 58431 58812 481-510 1.000000 66.3 petA-psbJ_p1 petA-psbJ 1 AGAAACAGTTCGAGAAGGTTCA CTGGATTGGGTTCATCTCTGT 22 21 40.9 47.6 57.6 57.4 11.7 0.0 4.043491 61400 62058 726-1147 1.000000 58.3 petA-psbJ_p2 petA-psbJ 2 TCGAGAAGGTTCAATTGTCAGA CTGGATTGGGTTCATCTCTGT 22 21 40.9 47.6 57.4 57.4 5.1 0.0 4.242127 61400 62058 717-1138 1.000000 56.8 petA-psbJ_p3 petA-psbJ 3 AGAAACAGTTCGAGAAGGTTCA TGGATTGGGTTCATCTCTGT 22 20 40.9 45.0 57.6 56.1 11.7 0.0 4.280043 61400 62058 725-1146 1.000000 56.5 petA-psbJ_p4 petA-psbJ 4 AGAAACAGTTCGAGAAGGTTCA TCTGGATTGGGTTCATCTCTG 22 21 40.9 47.6 57.6 57.1 11.7 0.0 4.322288 61400 62058 727-1148 1.000000 56.1 petA-psbJ_p5 petA-psbJ 5 TCGAGAAGGTTCAATTGTCAGA TGGATTGGGTTCATCTCTGT 22 20 40.9 45.0 57.4 56.1 5.1 0.0 4.478679 61400 62058 716-1137 1.000000 54.9 ndhF-rpl32_p1 ndhF-rpl32 1 CCGTGTGAACCAAAACCCTA CAAAGCTTTTAAGGCTGCCC 20 20 50.0 50.0 58.0 57.9 0.0 16.6 0.114518 111331 112211 1441-1759 1.000000 90.2 ndhF-rpl32_p2 ndhF-rpl32 2 GTTGCCGACGGAAAAAGAAG AGCTTTTAAGGCTGCCCAAT 20 20 50.0 45.0 58.0 58.1 20.5 16.6 0.089638 111331 112211 1072-1193 0.375000 61.2 ndhF-rpl32_p3 ndhF-rpl32 3 TGCCGACGGAAAAAGAAGAA AGCTTTTAAGGCTGCCCAAT 20 20 45.0 45.0 58.0 58.1 20.5 16.6 0.100927 111331 112211 1070-1191 0.375000 61.1 ndhF-rpl32_p4 ndhF-rpl32 4 TTGCCGACGGAAAAAGAAGA AGCTTTTAAGGCTGCCCAAT 20 20 45.0 45.0 58.0 58.1 20.5 16.6 0.100927 111331 112211 1071-1192 0.375000 61.1 ndhF-rpl32_p5 ndhF-rpl32 5 CCGTGTGAACCAAAACCCTA AGCTTTTAAGGCTGCCCAAT 20 20 50.0 45.0 58.0 58.1 0.0 16.6 0.087132 111331 112211 1478-1599 0.375000 60.7 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 20 20 55.0 50.0 58.1 58.0 1.3 0.0 0.096773 112377 112973 727-1032 0.750000 74.3 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 ATTGGGCAGCCTTAAAAGCT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.1 58.0 12.4 0.0 0.065054 112377 112973 718-782 0.625000 67.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 ATTGGGCAGCCTTAAAAGCT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 58.1 58.0 12.4 0.0 0.066425 112377 112973 719-783 0.625000 67.7 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GGGCAGCCTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 57.9 58.0 13.5 0.0 0.092440 112377 112973 715-779 0.625000 67.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GGGCAGCCTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 57.9 58.0 13.5 0.0 0.093810 112377 112973 716-780 0.625000 67.4 rps15-ycf1_p1 rps15-ycf1 1 GACCCCTTGTTGTCTTCGTT ATCGATCCCATCCGTTTGTC 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.277321 122211 122639 966-1013 0.500000 65.0 rps15-ycf1_p2 rps15-ycf1 2 GGTGCTGAGGACCCATATTG ATCGATCCCATCCGTTTGTC 20 20 55.0 50.0 58.0 57.8 12.6 0.0 0.267621 122211 122639 1326-1373 0.500000 64.5 rps15-ycf1_p3 rps15-ycf1 3 ATGAGTCGAAGAACACCGTG ATCGATCCCATCCGTTTGTC 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.306486 122211 122639 1354-1401 0.500000 64.2 rps15-ycf1_p4 rps15-ycf1 4 GGTCATTTCGTGTAGCTGGT ATCGATCCCATCCGTTTGTC 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.335256 122211 122639 1296-1343 0.500000 64.0 rps15-ycf1_p5 rps15-ycf1 5 TAGCTGGTGCAGTCATAGGT ATCGATCCCATCCGTTTGTC 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.374478 122211 122639 1284-1331 0.500000 63.8 ycf1_p1 ycf1 1 TGGGGTCATACCACCTTCAT GCTGAGGACTTCGATGAGTG 20 20 50.0 55.0 58.0 58.1 0.0 5.2 0.100273 124847 124966 1377-1377 0.625000 65.4 ycf1_p2 ycf1 2 TGGGGTCATACCACCTTCAT CCCTTCCCTTTGGTTCTGTC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.109133 124847 124966 1133-1133 0.625000 65.4 ycf1_p3 ycf1 3 TGGGGTCATACCACCTTCAT CCTTCCCTTTGGTTCTGTCC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.109133 124847 124966 1132-1132 0.625000 65.4 ycf1_p4 ycf1 4 TGGGGTCATACCACCTTCAT CTTCCCTTTGGTTCTGTCCC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.109133 124847 124966 1131-1131 0.625000 65.4 ycf1_p5 ycf1 5 TGGGGTCATACCACCTTCAT GAAACCTTGGCACAGATCCA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.110491 124847 124966 1240-1240 0.625000 65.4