primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	atpA-atpF	1	TACTTGAAGGACGGTACCGA	TGTTTGGGGCGATGAAAGAA	20	20	50.0	45.0	57.8	58.0	39.5	0.0	0.210533	13192	13250	196-200	1.000000	79.9
atpA-atpF_p2	atpA-atpF	2	GCCTACTTGAAGGACGGTAC	TGTTTGGGGCGATGAAAGAA	20	20	55.0	45.0	57.7	58.0	0.0	0.0	0.302237	13192	13250	199-203	1.000000	79.2
atpA-atpF_p3	atpA-atpF	3	TACTTGAAGGACGGTACCGA	ATGTTTGGGGCGATGAAAGA	20	20	50.0	45.0	57.8	57.8	39.5	0.0	0.413203	13192	13250	197-201	1.000000	78.3
atpA-atpF_p4	atpA-atpF	4	ACTTGAAGGACGGTACCGAT	TGTTTGGGGCGATGAAAGAA	20	20	50.0	45.0	58.4	58.0	39.5	0.0	0.458000	13192	13250	195-199	1.000000	78.0
atpA-atpF_p5	atpA-atpF	5	TACTTGAAGGACGGTACCGA	CATGTTTGGGGCGATGAAAG	20	20	50.0	50.0	57.8	57.7	39.5	0.0	0.481527	13192	13250	198-202	1.000000	77.7
ycf1_p1	ycf1	1	GGATCAGTTCGCTCAAACCA	TTTTCTCCAAAGGGTGTCCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.215930	118680	118829	709-709	0.500000	58.3
ycf1_p2	ycf1	2	TGGATCAGTTCGCTCAAACC	TTTTCTCCAAAGGGTGTCCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.215930	118680	118829	710-710	0.500000	58.3
ycf1_p3	ycf1	3	GGATCAGTTCGCTCAAACCA	AAAGGGTGTCCGCTCTTTTT	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.258955	118680	118829	701-701	0.500000	57.9
ycf1_p4	ycf1	4	TGGATCAGTTCGCTCAAACC	AAAGGGTGTCCGCTCTTTTT	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.258955	118680	118829	702-702	0.500000	57.9
ycf1_p5	ycf1	5	ACCTGGAGCTTTGGTTCTTC	TTTTCTCCAAAGGGTGTCCG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.302673	118680	118829	963-963	0.500000	57.6
ycf1-rps15_p1	ycf1-rps15	1	CGAGAAGCGGGTTATGTTGA	ACGATTAGGCGAAGAACACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010553	119247	119635	1281-1281	0.500000	59.9
ycf1-rps15_p2	ycf1-rps15	2	CGAGAAGCGGGTTATGTTGA	GATTAGGCGAAGAACACCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010553	119247	119635	1279-1279	0.500000	59.9
ycf1-rps15_p3	ycf1-rps15	3	CGGACACCCTTTGGAGAAAA	ACGATTAGGCGAAGAACACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030311	119247	119635	1060-1060	0.500000	59.8
ycf1-rps15_p4	ycf1-rps15	4	CGGACACCCTTTGGAGAAAA	GATTAGGCGAAGAACACCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030311	119247	119635	1058-1058	0.500000	59.8
ycf1-rps15_p5	ycf1-rps15	5	CGAGAAGCGGGTTATGTTGA	ATAAGCTAGCAGACGTTGGC	20	20	50.0	50.0	58.0	58.1	0.0	18.8	0.063258	119247	119635	1013-1013	0.500000	59.5
ndhH_p1	ndhH	1	GCCAACGTCTGCTAGCTTAT	AGCCTCAATCCACCCTTACT	20	20	50.0	50.0	58.1	58.0	17.2	0.0	0.086087	120013	121194	2128-2128	0.500000	59.3
ndhH_p2	ndhH	2	GCCAACGTCTGCTAGCTTAT	TGAGCCTCAATCCACCCTTA	20	20	50.0	50.0	58.1	58.0	17.2	0.0	0.093261	120013	121194	2130-2130	0.500000	59.3
ndhH_p3	ndhH	3	GCCAACGTCTGCTAGCTTAT	CCTCAATCCACCCTTACTGC	20	20	50.0	55.0	58.1	58.0	17.2	0.0	0.102798	120013	121194	2126-2126	0.500000	59.2
ndhH_p4	ndhH	4	GCCAACGTCTGCTAGCTTAT	GCCTCAATCCACCCTTACTG	20	20	50.0	55.0	58.1	58.0	17.2	0.0	0.102798	120013	121194	2127-2127	0.500000	59.2
ndhH_p5	ndhH	5	TGCGGATCGGTTGAATTTCA	AGCCTCAATCCACCCTTACT	20	20	45.0	50.0	58.2	58.0	1.9	0.0	0.212619	120013	121194	2252-2252	0.500000	58.3
ndhA_p1	ndhA	1	AGAGTTGAAGCCCCAAAAGG	TGCGCGTATTGTTTGTTGAC	20	20	50.0	45.0	58.0	58.0	0.0	8.4	0.026620	121196	123434	2579-2579	0.500000	59.8
ndhA_p2	ndhA	2	AGTTGAAGCCCCAAAAGGAG	TGCGCGTATTGTTTGTTGAC	20	20	50.0	45.0	58.0	58.0	0.0	8.4	0.026620	121196	123434	2577-2577	0.500000	59.8
ndhA_p3	ndhA	3	GAGTTGAAGCCCCAAAAGGA	TGCGCGTATTGTTTGTTGAC	20	20	50.0	45.0	58.0	58.0	0.0	8.4	0.030168	121196	123434	2578-2578	0.500000	59.8
ndhA_p4	ndhA	4	GTTGAAGCCCCAAAAGGAGA	TGCGCGTATTGTTTGTTGAC	20	20	50.0	45.0	58.0	58.0	0.0	8.4	0.030168	121196	123434	2576-2576	0.500000	59.8
ndhA_p5	ndhA	5	TCCCACTTTTGAATTGCCGA	TGCGCGTATTGTTTGTTGAC	20	20	45.0	45.0	58.0	58.0	0.0	8.4	0.037251	121196	123434	2616-2616	0.500000	59.7
ndhI_p1	ndhI	1	GTTGATATAGTTGGGGCGCA	TGAACCGACCGGGAATTTTT	20	20	50.0	45.0	58.0	58.0	10.8	13.4	0.054141	123522	124034	1444-1444	0.500000	59.6
ndhI_p2	ndhI	2	GTTGATATAGTTGGGGCGCA	TTGAACCGACCGGGAATTTT	20	20	50.0	45.0	58.0	58.0	10.8	13.4	0.054141	123522	124034	1445-1445	0.500000	59.6
ndhI_p3	ndhI	3	GTTGATATAGTTGGGGCGCA	ACCCCATCACCAATAGTCCA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.058397	123522	124034	1795-1795	0.500000	59.5
ndhI_p4	ndhI	4	GTTGATATAGTTGGGGCGCA	TAATCCCGTACCACGACGTA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.069465	123522	124034	1869-1869	0.500000	59.4
ndhI_p5	ndhI	5	GTTGATATAGTTGGGGCGCA	ACGGAACCCTAGGAATTGGA	20	20	50.0	50.0	58.0	58.0	10.8	11.5	0.077074	123522	124034	1141-1141	0.500000	59.4
ndhI-ndhG_p1	ndhI-ndhG	1	GTCAACAAACAATACGCGCA	ACCCCATCACCAATAGTCCA	20	20	45.0	50.0	58.0	58.0	5.7	0.0	0.045735	124035	124408	1155-1155	0.500000	59.6
ndhI-ndhG_p2	ndhI-ndhG	2	GTCAACAAACAATACGCGCA	TAATCCCGTACCACGACGTA	20	20	45.0	50.0	58.0	58.0	5.7	0.0	0.056803	124035	124408	1229-1229	0.500000	59.5
ndhI-ndhG_p3	ndhI-ndhG	3	ACGACCGTCACGAATTGAAT	ACCCCATCACCAATAGTCCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.094738	124035	124408	801-801	0.500000	59.2
ndhI-ndhG_p4	ndhI-ndhG	4	ACGACCGTCACGAATTGAAT	TAATCCCGTACCACGACGTA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.105805	124035	124408	875-875	0.500000	59.2
ndhI-ndhG_p5	ndhI-ndhG	5	TGTGAAGTATGTGTTCGCGT	ACCCCATCACCAATAGTCCA	20	20	45.0	50.0	57.9	58.0	5.8	0.0	0.163198	124035	124408	991-991	0.500000	58.7
ndhG_p1	ndhG	1	AAAAATTCCCGGTCGGTTCA	CAAGACACATCAGAGCACGA	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124409	124945	977-977	0.500000	59.3
ndhG_p2	ndhG	2	AAAAATTCCCGGTCGGTTCA	TCAAGACACATCAGAGCACG	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124409	124945	978-978	0.500000	59.3
ndhG_p3	ndhG	3	AAAATTCCCGGTCGGTTCAA	CAAGACACATCAGAGCACGA	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124409	124945	976-976	0.500000	59.3
ndhG_p4	ndhG	4	AAAATTCCCGGTCGGTTCAA	TCAAGACACATCAGAGCACG	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124409	124945	977-977	0.500000	59.3
ndhG_p5	ndhG	5	AAAATTCCCGGTCGGTTCAA	AGACACATCAGAGCACGAAC	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.087623	124409	124945	974-974	0.500000	59.3
ndhG-ndhE_p1	ndhG-ndhE	1	AAAAATTCCCGGTCGGTTCA	CAAGACACATCAGAGCACGA	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124946	125213	977-977	0.500000	59.3
ndhG-ndhE_p2	ndhG-ndhE	2	AAAAATTCCCGGTCGGTTCA	TCAAGACACATCAGAGCACG	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124946	125213	978-978	0.500000	59.3
ndhG-ndhE_p3	ndhG-ndhE	3	AAAATTCCCGGTCGGTTCAA	CAAGACACATCAGAGCACGA	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124946	125213	976-976	0.500000	59.3
ndhG-ndhE_p4	ndhG-ndhE	4	AAAATTCCCGGTCGGTTCAA	TCAAGACACATCAGAGCACG	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.086196	124946	125213	977-977	0.500000	59.3
ndhG-ndhE_p5	ndhG-ndhE	5	AAAATTCCCGGTCGGTTCAA	AGACACATCAGAGCACGAAC	20	20	45.0	50.0	58.0	57.9	8.7	0.0	0.087623	124946	125213	974-974	0.500000	59.3
ndhE_p1	ndhE	1	TGGACTATTGGTGATGGGGT	TTAAAGAACCCGCGGCTATC	20	20	50.0	50.0	58.0	58.1	0.0	22.8	0.076159	125214	125519	1539-1539	0.500000	59.4
ndhE_p2	ndhE	2	TGGACTATTGGTGATGGGGT	TGCTTTACATCCGTCCCAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.128141	125214	125519	1174-1174	0.500000	59.0
ndhE_p3	ndhE	3	TGGACTATTGGTGATGGGGT	CTTACAACCAACGCAGTCCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.141238	125214	125519	1222-1222	0.500000	58.9
ndhE_p4	ndhE	4	TGGACTATTGGTGATGGGGT	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.146061	125214	125519	1168-1168	0.500000	58.8
ndhE_p5	ndhE	5	TGGACTATTGGTGATGGGGT	TTACAACCAACGCAGTCCTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.146493	125214	125519	1221-1221	0.500000	58.8
ndhD_p1	ndhD	1	GCGTGTCCCACAGATGTATT	GCTAATGAGGCATGGGGATC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.192595	126142	127671	2380-2380	0.500000	58.5
ndhD_p2	ndhD	2	GCGTGTCCCACAGATGTATT	GGGCGTCTGGTCACAATTAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.208101	126142	127671	2016-2016	0.500000	58.3
ndhD_p3	ndhD	3	GCGTGTCCCACAGATGTATT	GGGAGCAGTATGGGCTAATG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.215303	126142	127671	2393-2393	0.500000	58.3
ndhD_p4	ndhD	4	GCGTGTCCCACAGATGTATT	TTCCGCAATTGTGGCTTCTA	20	20	50.0	45.0	58.0	57.8	0.0	29.6	0.216839	126142	127671	2258-2258	0.500000	58.3
ndhD_p5	ndhD	5	CTTGGGACGGATGTAAAGCA	GCTAATGAGGCATGGGGATC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.276370	126142	127671	2349-2349	0.500000	57.8
ndhD-ccsA_p1	ndhD-ccsA	1	GAAGTATTACCGCGTGCTCA	GCTAATGAGGCATGGGGATC	20	20	50.0	55.0	58.1	58.2	4.9	0.0	0.251147	127672	127968	1227-1227	0.500000	58.0
ndhD-ccsA_p2	ndhD-ccsA	2	GAAGTATTACCGCGTGCTCA	GGGAGCAGTATGGGCTAATG	20	20	50.0	55.0	58.1	57.8	4.9	0.0	0.273855	127672	127968	1240-1240	0.500000	57.8
ndhD-ccsA_p3	ndhD-ccsA	3	GAAGTATTACCGCGTGCTCA	TTCCGCAATTGTGGCTTCTA	20	20	50.0	45.0	58.1	57.8	4.9	29.6	0.275391	127672	127968	1105-1105	0.500000	57.8
ndhD-ccsA_p4	ndhD-ccsA	4	CGGACTGGCTATCCCAATTC	GCTAATGAGGCATGGGGATC	20	20	55.0	55.0	58.1	58.2	2.5	0.0	0.284185	127672	127968	931-931	0.500000	57.7
ndhD-ccsA_p5	ndhD-ccsA	5	CGGACTGGCTATCCCAATTC	GGGAGCAGTATGGGCTAATG	20	20	55.0	55.0	58.1	57.8	2.5	0.0	0.306892	127672	127968	944-944	0.500000	57.5
ccsA_p1	ccsA	1	CGGACTGGCTATCCCAATTC	GTGAAATCGGTAGACACGCT	20	20	55.0	50.0	58.1	58.0	2.5	0.0	0.123595	127969	128928	1845-1845	0.500000	59.0
ccsA_p2	ccsA	2	CGGACTGGCTATCCCAATTC	TGAAATCGGTAGACACGCTG	20	20	55.0	50.0	58.1	58.0	2.5	0.0	0.124966	127969	128928	1844-1844	0.500000	59.0
ccsA_p3	ccsA	3	CGGACTGGCTATCCCAATTC	AACCGGTACAACGATTCGAG	20	20	55.0	50.0	58.1	58.0	2.5	8.0	0.129067	127969	128928	2090-2090	0.500000	59.0
ccsA_p4	ccsA	4	CGGACTGGCTATCCCAATTC	CCGGTACAACGATTCGAGTT	20	20	55.0	50.0	58.1	58.0	2.5	14.0	0.129067	127969	128928	2088-2088	0.500000	59.0
ccsA_p5	ccsA	5	TTATCTCGACGAAATGGGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.160012	127969	128928	1863-1863	0.500000	58.7
trnL-UAG_p1	trnL-UAG	1	ACTTGGAGAGCGGGTACTAA	AACCGGTACAACGATTCGAG	20	20	50.0	50.0	57.8	58.0	0.0	8.0	0.240264	129037	129116	822-822	0.500000	58.1
trnL-UAG_p2	trnL-UAG	2	ACTTGGAGAGCGGGTACTAA	CCGGTACAACGATTCGAGTT	20	20	50.0	50.0	57.8	58.0	0.0	14.0	0.240264	129037	129116	820-820	0.500000	58.1
trnL-UAG_p3	trnL-UAG	3	ACTTGGAGAGCGGGTACTAA	TGGGTAGCGTTGAAAGCTTT	20	20	50.0	45.0	57.8	58.0	0.0	12.5	0.251303	129037	129116	1313-1313	0.500000	58.0
trnL-UAG_p4	trnL-UAG	4	ACTTGGAGAGCGGGTACTAA	TTGGGTAGCGTTGAAAGCTT	20	20	50.0	45.0	57.8	58.0	0.0	11.4	0.251303	129037	129116	1314-1314	0.500000	58.0
trnL-UAG_p5	trnL-UAG	5	GACTTGGAGAGCGGGTACTA	CCGGTACAACGATTCGAGTT	20	20	55.0	50.0	58.2	58.0	0.0	14.0	0.253110	129037	129116	821-821	0.500000	58.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	TGGGTAGCGTTGAAAGCTTT	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.032171	129117	129735	756-756	0.500000	59.7
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	AGCGTGTCTACCGATTTCAC	TTGGGTAGCGTTGAAAGCTT	20	20	50.0	45.0	58.0	58.0	0.0	11.4	0.032171	129117	129735	757-757	0.500000	59.7
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CAGCGTGTCTACCGATTTCA	TGGGTAGCGTTGAAAGCTTT	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.033541	129117	129735	757-757	0.500000	59.7
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	TTGGGTAGCGTTGAAAGCTT	20	20	50.0	45.0	58.0	58.0	0.0	11.4	0.033541	129117	129735	758-758	0.500000	59.7
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCGTGTCTACCGATTTCAC	AAAGTAGAAGCCCCACTCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054110	129117	129735	1212-1212	0.500000	59.6
rpl32-ndhF_p1	rpl32-ndhF	1	AACTCGAATCGTTGTACCGG	AAAGTAGAAGCCCCACTCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059582	129919	130214	969-969	0.500000	59.5
rpl32-ndhF_p2	rpl32-ndhF	2	CTCGAATCGTTGTACCGGTT	AAAGTAGAAGCCCCACTCCT	20	20	50.0	50.0	58.0	58.0	15.0	0.0	0.059582	129919	130214	967-967	0.500000	59.5
rpl32-ndhF_p3	rpl32-ndhF	3	AACTCGAATCGTTGTACCGG	TGTTGCCGACGGAAAAAGTA	20	20	50.0	45.0	58.0	58.0	0.0	20.5	0.062474	129919	130214	983-983	0.500000	59.5
rpl32-ndhF_p4	rpl32-ndhF	4	CTCGAATCGTTGTACCGGTT	TGTTGCCGACGGAAAAAGTA	20	20	50.0	45.0	58.0	58.0	15.0	20.5	0.062474	129919	130214	981-981	0.500000	59.5
rpl32-ndhF_p5	rpl32-ndhF	5	CTCGAATCGTTGTACCGGTT	TACATGAAGGGGGAATTGCG	20	20	50.0	50.0	58.0	57.9	15.0	0.0	0.064636	129919	130214	1623-1623	0.500000	59.5
ndhF_p1	ndhF	1	AAAGCTTTCAACGCTACCCA	ATCCAGCAAGTTTGGCAACT	20	20	45.0	45.0	58.0	58.3	5.7	0.0	0.315999	130215	132434	2812-2812	0.500000	57.5
ndhF_p2	ndhF	2	AAGCTTTCAACGCTACCCAA	ATCCAGCAAGTTTGGCAACT	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.315999	130215	132434	2811-2811	0.500000	57.5
ndhF_p3	ndhF	3	CAACGCTACCCAATATCCCC	ATCCAGCAAGTTTGGCAACT	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.399449	130215	132434	2804-2804	0.500000	56.8
ndhF_p4	ndhF	4	TCGAGTCAGGCTGATTCAGA	ATCCAGCAAGTTTGGCAACT	20	20	50.0	45.0	58.2	58.3	31.2	0.0	0.452235	130215	132434	2920-2920	0.500000	56.4
ndhF_p5	ndhF	5	GCTACCCAATATCCCCGTTC	ATCCAGCAAGTTTGGCAACT	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.472091	130215	132434	2800-2800	0.500000	56.2