primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ycf1-rps15	1	AATTATGGAACCTGCCCGTC	CCGCAGACCTCTCTGAGATA	20	20	50.0	55.0	57.9	58.0	0.0	8.0	0.078517	113175	113569	1074-1074	0.500000	59.4
ycf1-rps15_p2	ycf1-rps15	2	AATTATGGAACCTGCCCGTC	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.111405	113175	113569	1350-1350	0.500000	59.1
ycf1-rps15_p3	ycf1-rps15	3	TATGGAACCTGCCCGTCTAT	CCGCAGACCTCTCTGAGATA	20	20	50.0	55.0	57.9	58.0	0.0	8.0	0.122845	113175	113569	1071-1071	0.500000	59.0
ycf1-rps15_p4	ycf1-rps15	4	AATTATGGAACCTGCCCGTC	AATAAGCCAGCAACCGTTGA	20	20	50.0	45.0	57.9	58.1	0.0	15.6	0.148495	113175	113569	1111-1111	0.500000	58.8
ycf1-rps15_p5	ycf1-rps15	5	TATGGAACCTGCCCGTCTAT	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.155733	113175	113569	1347-1347	0.500000	58.8
rps15_p1	rps15	1	GCAAGTAACCCAATGCACAC	GTAAATCAGCAGCGACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113570	113809	1169-1169	0.500000	59.4
rps15_p2	rps15	2	GCAAGTAACCCAATGCACAC	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113570	113809	1168-1168	0.500000	59.4
rps15_p3	rps15	3	GCAAGTAACCCAATGCACAC	TGAGGTAAATCAGCAGCGAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113570	113809	1173-1173	0.500000	59.4
rps15_p4	rps15	4	TTTCTGCTGTCCGTGATCTG	GTAAATCAGCAGCGACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.094755	113570	113809	1207-1207	0.500000	59.2
rps15_p5	rps15	5	TTTCTGCTGTCCGTGATCTG	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.094755	113570	113809	1206-1206	0.500000	59.2
rps15-ndhH_p1	rps15-ndhH	1	GCAAGTAACCCAATGCACAC	GTAAATCAGCAGCGACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113810	113938	1169-1169	0.500000	59.4
rps15-ndhH_p2	rps15-ndhH	2	GCAAGTAACCCAATGCACAC	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113810	113938	1168-1168	0.500000	59.4
rps15-ndhH_p3	rps15-ndhH	3	GCAAGTAACCCAATGCACAC	TGAGGTAAATCAGCAGCGAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.074718	113810	113938	1173-1173	0.500000	59.4
rps15-ndhH_p4	rps15-ndhH	4	TTTCTGCTGTCCGTGATCTG	GTAAATCAGCAGCGACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.094755	113810	113938	1207-1207	0.500000	59.2
rps15-ndhH_p5	rps15-ndhH	5	TTTCTGCTGTCCGTGATCTG	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.094755	113810	113938	1206-1206	0.500000	59.2
ndhH_p1	ndhH	1	GCAAGTAACCCAATGCACAC	CGTTGTTGTATACCCGCAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.065525	113939	115120	2032-2032	0.500000	59.5
ndhH_p2	ndhH	2	TTTCTGCTGTCCGTGATCTG	CGTTGTTGTATACCCGCAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.085561	113939	115120	2070-2070	0.500000	59.3
ndhH_p3	ndhH	3	TCAACGGTTGCTGGCTTATT	CGTTGTTGTATACCCGCAGA	20	20	45.0	50.0	58.1	58.0	15.9	0.0	0.096665	113939	115120	1549-1549	0.500000	59.2
ndhH_p4	ndhH	4	GCAAGTAACCCAATGCACAC	CCTGACATAAGAAGGCCGAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.123248	113939	115120	2296-2296	0.500000	59.0
ndhH_p5	ndhH	5	GCAAGTAACCCAATGCACAC	GACATAAGAAGGCCGACAGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.123248	113939	115120	2293-2293	0.500000	59.0
ndhA_p1	ndhA	1	CGGCCACCGGGATTTATTAA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	6.3	0.0	0.068115	115122	117289	2438-2438	0.500000	59.5
ndhA_p2	ndhA	2	TCAACAGGCCCTAGAAGGAA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	115122	117289	2676-2676	0.500000	59.4
ndhA_p3	ndhA	3	TTCAACAGGCCCTAGAAGGA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	115122	117289	2677-2677	0.500000	59.4
ndhA_p4	ndhA	4	GGCAAAAAGAGGGGGATTCA	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101015	115122	117289	2749-2749	0.500000	59.2
ndhA_p5	ndhA	5	TGGCAAAAAGAGGGGGATTC	TGACCAATATACCTTGCGGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101015	115122	117289	2750-2750	0.500000	59.2
ndhI_p1	ndhI	1	AGCCGAGTGTGAACGATTAC	AGACCACGGAAGTTACTCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	117368	117868	1682-1682	0.500000	59.9
ndhI_p2	ndhI	2	AGCCGAGTGTGAACGATTAC	AGACTTGTACAGACCACGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016709	117368	117868	1692-1692	0.500000	59.9
ndhI_p3	ndhI	3	TTTGTGACCTTTGAGGAGCC	AGACCACGGAAGTTACTCCA	20	20	50.0	50.0	58.0	58.0	0.1	0.0	0.024114	117368	117868	1888-1888	0.500000	59.8
ndhI_p4	ndhI	4	TTTGTGACCTTTGAGGAGCC	AGACTTGTACAGACCACGGA	20	20	50.0	50.0	58.0	58.0	0.1	0.0	0.027622	117368	117868	1898-1898	0.500000	59.8
ndhI_p5	ndhI	5	GACCTTTGAGGAGCCGTATG	AGACCACGGAAGTTACTCCA	20	20	55.0	50.0	58.1	58.0	0.1	0.0	0.064045	117368	117868	1883-1883	0.500000	59.5
ndhG_p1	ndhG	1	TTGATCACATCCGAGCGTTT	CGATGAGTTCCCCGAGTTTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.188068	118238	118768	1935-1935	0.500000	58.5
ndhG_p2	ndhG	2	TTGATCACATCCGAGCGTTT	GAATCGATGAGTTCCCCGAG	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.234841	118238	118768	1939-1939	0.500000	58.1
ndhG_p3	ndhG	3	TCAAATTGCTTTGGGTCGGT	CGATGAGTTCCCCGAGTTTT	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.396256	118238	118768	1660-1660	0.500000	56.8
ndhG_p4	ndhG	4	AATTGATCACATCCGAGCGT	CGATGAGTTCCCCGAGTTTT	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.410687	118238	118768	1937-1937	0.500000	56.7
ndhG_p5	ndhG	5	ATTGATCACATCCGAGCGTT	CGATGAGTTCCCCGAGTTTT	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.410687	118238	118768	1936-1936	0.500000	56.7
psaC_p1	psaC	1	AAAACTCGGGGAACTCATCG	TCTTTCCTCCCCACATGGAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.109141	119529	119774	949-949	0.500000	59.1
psaC_p2	psaC	2	CTCGGGGAACTCATCGATTC	TCTTTCCTCCCCACATGGAT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.155914	119529	119774	945-945	0.500000	58.8
psaC_p3	psaC	3	AAAACTCGGGGAACTCATCG	GAGACGTTTCTTTCCTCCCC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.190982	119529	119774	957-957	0.500000	58.5
psaC_p4	psaC	4	AAAACTCGGGGAACTCATCG	TTCCAAAGCCACAGGATACG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.202712	119529	119774	1104-1104	0.500000	58.4
psaC_p5	psaC	5	CTCGGGGAACTCATCGATTC	GAGACGTTTCTTTCCTCCCC	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.237755	119529	119774	953-953	0.500000	58.1
ndhD_p1	ndhD	1	TCTGCTCCAAGAACAGAGGA	AGCAAATTCCGCAATTGTGG	20	20	50.0	45.0	58.0	57.9	0.0	28.7	0.098623	119895	121397	2128-2128	0.500000	59.2
ndhD_p2	ndhD	2	AAAACTCGGGGAACTCATCG	AGCAAATTCCGCAATTGTGG	20	20	50.0	45.0	57.9	57.9	0.0	28.7	0.177929	119895	121397	2356-2356	0.500000	58.6
ndhD_p3	ndhD	3	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	119895	121397	2259-2259	0.500000	58.5
ndhD_p4	ndhD	4	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	119895	121397	2260-2260	0.500000	58.5
ndhD_p5	ndhD	5	TGATACCTTGGGACGGATGT	AGCAAATTCCGCAATTGTGG	20	20	50.0	45.0	58.1	57.9	0.0	28.7	0.209037	119895	121397	2166-2166	0.500000	58.3
ndhD-ccsA_p1	ndhD-ccsA	1	CTCCCGGCCAACGTAATTTA	AGCAAATTCCGCAATTGTGG	20	20	50.0	45.0	58.0	57.9	2.2	28.7	0.110170	121398	121655	999-999	0.500000	59.1
ndhD-ccsA_p2	ndhD-ccsA	2	CTCCCGGCCAACGTAATTTA	ACCCAAGGCTTTGCTACTTC	20	20	50.0	50.0	58.0	58.1	2.2	2.4	0.115946	121398	121655	1523-1523	0.500000	59.1
ndhD-ccsA_p3	ndhD-ccsA	3	GTTTTTGATTCTGGACCGCG	AGCAAATTCCGCAATTGTGG	20	20	50.0	45.0	58.0	57.9	0.0	28.7	0.119420	121398	121655	523-523	0.500000	59.0
ndhD-ccsA_p4	ndhD-ccsA	4	GTTTTTGATTCTGGACCGCG	ACCCAAGGCTTTGCTACTTC	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.125196	121398	121655	1047-1047	0.500000	59.0
ndhD-ccsA_p5	ndhD-ccsA	5	CTCCCGGCCAACGTAATTTA	CGGCTCTTCTTTGTGGATCA	20	20	50.0	50.0	58.0	57.9	2.2	0.0	0.126145	121398	121655	1405-1405	0.500000	59.0
ccsA_p1	ccsA	1	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	121656	122624	1550-1550	0.500000	59.6
ccsA_p2	ccsA	2	GTTTTTGATTCTGGACCGCG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	121656	122624	1549-1549	0.500000	59.6
ccsA_p3	ccsA	3	GTTTTTGATTCTGGACCGCG	AATTTTGGTGGTGGGGAGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045935	121656	122624	2090-2090	0.500000	59.6
ccsA_p4	ccsA	4	CATGGCTTTATTGGTGCTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.077263	121656	122624	1898-1898	0.500000	59.4
ccsA_p5	ccsA	5	CATGGCTTTATTGGTGCTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.078633	121656	122624	1897-1897	0.500000	59.4
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	GGATATGAGGCGGCGTTAAA	20	20	50.0	50.0	58.0	58.1	0.0	9.4	0.134133	122814	123582	895-895	0.500000	58.9
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	GGATATGAGGCGGCGTTAAA	20	20	50.0	50.0	58.0	58.1	0.0	9.4	0.135503	122814	123582	896-896	0.500000	58.9
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	GAAGTAGCAAAGCCTTGGGT	GGATATGAGGCGGCGTTAAA	20	20	50.0	50.0	58.1	58.1	0.0	9.4	0.215245	122814	123582	1398-1398	0.500000	58.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	TGATCCACAAAGAAGAGCCG	GGATATGAGGCGGCGTTAAA	20	20	50.0	50.0	57.9	58.1	0.0	9.4	0.225443	122814	123582	1516-1516	0.500000	58.2
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	GGATATGAGGCGGCGTTAAA	20	20	50.0	50.0	57.9	58.1	0.0	9.4	0.250579	122814	123582	905-905	0.500000	58.0
rpl32-ndhF_p1	rpl32-ndhF	1	GACTCCCCACCACCAAAATT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.042096	123748	124734	1509-1509	0.500000	59.7
rpl32-ndhF_p2	rpl32-ndhF	2	GACTCCCCACCACCAAAATT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	58.0	58.0	0.0	17.5	0.053385	123748	124734	1507-1507	0.500000	59.6
rpl32-ndhF_p3	rpl32-ndhF	3	GACTCCCCACCACCAAAATT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	58.0	58.0	0.0	17.5	0.053385	123748	124734	1508-1508	0.500000	59.6
rpl32-ndhF_p4	rpl32-ndhF	4	AATAAGACTCCCCACCACCA	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.9	58.0	0.0	17.5	0.086161	123748	124734	1514-1514	0.500000	59.3
rpl32-ndhF_p5	rpl32-ndhF	5	ATAAGACTCCCCACCACCAA	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.9	58.0	0.0	17.5	0.086161	123748	124734	1513-1513	0.500000	59.3
ndhF_p1	ndhF	1	CAGGTAAGATCCGGCGAATC	GCCGAGAAGAAGGTATCAGC	20	20	55.0	55.0	58.2	58.1	9.3	0.0	0.356092	124735	126966	2752-2752	0.500000	57.2
ndhF_p2	ndhF	2	CAGGTAAGATCCGGCGAATC	CTAGCACTGGGTAGACCTCA	20	20	55.0	55.0	58.2	57.9	9.3	0.0	0.360872	124735	126966	2668-2668	0.500000	57.1
ndhF_p3	ndhF	3	CAGGTAAGATCCGGCGAATC	TCGTTGTGGTCGGACTCTAT	20	20	55.0	50.0	58.2	58.2	9.3	8.2	0.385196	124735	126966	2856-2856	0.500000	56.9
ndhF_p4	ndhF	4	CAGGTAAGATCCGGCGAATC	ATCAATTCGGTCGTTGTGGT	20	20	55.0	45.0	58.2	57.8	9.3	0.0	0.394387	124735	126966	2866-2866	0.500000	56.8
ndhF_p5	ndhF	5	CAGGTAAGATCCGGCGAATC	AGAAGAAGGAGCCGAGAAGA	20	20	55.0	50.0	58.2	57.8	9.3	0.0	0.439278	124735	126966	2762-2762	0.500000	56.5