primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU_p1 trnK-UUU 1 TACCAATGTCAACCAAGCCA CGTGCGGTGTAATTCCATTG 20 20 45.0 50.0 57.3 57.8 0.0 0.0 0.838864 1377 3965 2759-2760 1.000000 73.4 trnK-UUU_p2 trnK-UUU 2 ACCAATGTCAACCAAGCCAG CGTGCGGTGTAATTCCATTG 20 20 50.0 50.0 58.7 57.8 0.0 0.0 0.841379 1377 3965 2758-2759 1.000000 73.4 trnK-UUU_p3 trnK-UUU 3 ATACCAATGTCAACCAAGCCA CGTGCGGTGTAATTCCATTG 21 20 42.9 50.0 57.8 57.8 0.0 0.0 1.340628 1377 3965 2760-2761 1.000000 69.4 trnK-UUU_p4 trnK-UUU 4 TACCAATGTCAACCAAGCCAG CGTGCGGTGTAATTCCATTG 21 20 47.6 50.0 58.5 57.8 0.0 0.0 1.658839 1377 3965 2759-2760 1.000000 66.8 trnK-UUU_p5 trnK-UUU 5 CAATGTCAACCAAGCCAGC CGTGCGGTGTAATTCCATTG 19 20 52.6 50.0 57.5 57.8 0.0 0.0 1.674830 1377 3965 2756-2757 1.000000 66.7 matK-trnK-UUU_p1 matK-trnK-UUU 1 ACTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 21 20 47.6 50.0 58.3 57.8 0.0 0.0 1.477007 3232 3927 864-865 1.000000 68.4 matK-trnK-UUU_p2 matK-trnK-UUU 2 ACTCCTGAAAGAGAAGTGGG CGTGCGGTGTAATTCCATTG 20 20 50.0 50.0 56.5 57.8 0.0 0.0 1.706995 3232 3927 864-865 1.000000 66.5 matK-trnK-UUU_p3 matK-trnK-UUU 3 CTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 20 20 50.0 50.0 56.5 57.8 0.0 0.0 1.706995 3232 3927 863-864 1.000000 66.5 matK-trnK-UUU_p4 matK-trnK-UUU 4 ACTCCTGAAAGAGAAGTGGGT TGGGTTGCTAACTCAATGGT 21 20 47.6 45.0 58.3 57.0 0.0 0.0 2.274236 3232 3927 805-806 1.000000 62.0 matK-trnK-UUU_p5 matK-trnK-UUU 5 ACTCCTGAAAGAGAAGTGGGTA CGTGCGGTGTAATTCCATTG 22 20 45.5 50.0 58.1 57.8 0.0 0.0 2.314458 3232 3927 864-865 1.000000 61.7 rps16_p1 rps16 1 AGCACGACATAAATCCAGTCA GGGTCAATTCCCTATACCACT 21 21 42.9 47.6 57.4 56.5 0.0 0.0 4.130238 4522 5608 1255-1255 1.000000 47.0 rps16_p2 rps16 2 AGCACGACATAAATCCAGTCA GGGTCAATTCCCTATACCACTT 21 22 42.9 45.5 57.4 57.2 0.0 0.0 4.400758 4522 5608 1255-1255 1.000000 44.9 rps16_p3 rps16 3 AGCACGACATAAATCCAGTCA TCCCTATACCACTTTGCAATGA 21 22 42.9 40.9 57.4 57.1 0.0 18.3 4.463507 4522 5608 1247-1247 1.000000 44.4 rps16_p4 rps16 4 ACGAGGGATACTAGTGATCCTC GGGTCAATTCCCTATACCACT 22 21 50.0 47.6 58.0 56.5 20.9 0.0 4.543924 4522 5608 1188-1188 1.000000 43.7 rps16_p5 rps16 5 AGCACGACATAAATCCAGTCAA GGGTCAATTCCCTATACCACT 22 21 40.9 47.6 58.1 56.5 0.0 0.0 4.582384 4522 5608 1255-1255 1.000000 43.4 trnfM-CAU-trnT-GGU_p1 trnfM-CAU-trnT-GGU 1 CCTCAAGGTTATGAGCCTCG CCATGGCATTACTCTACCACC 20 21 55.0 52.4 57.8 58.1 0.0 0.0 1.380020 13584 16352 2897-2917 1.000000 69.6 trnfM-CAU-trnT-GGU_p2 trnfM-CAU-trnT-GGU 2 CCTCGTGAGCTACCAAACTG CCATGGCATTACTCTACCACC 20 21 55.0 52.4 58.3 58.1 0.0 0.0 1.434011 13584 16352 2882-2902 1.000000 69.2 trnfM-CAU-trnT-GGU_p3 trnfM-CAU-trnT-GGU 3 CCTCAAGGTTATGAGCCTCG GACTTACGTCTTACCATGGCA 20 21 55.0 47.6 57.8 57.8 0.0 13.0 1.472281 13584 16352 2910-2930 1.000000 68.9 trnfM-CAU-trnT-GGU_p4 trnfM-CAU-trnT-GGU 4 CCTCAAGGTTATGAGCCTCG TGACTTACGTCTTACCATGGC 20 21 55.0 47.6 57.8 57.8 0.0 13.0 1.472281 13584 16352 2911-2931 1.000000 68.9 trnfM-CAU-trnT-GGU_p5 trnfM-CAU-trnT-GGU 5 CCTCGTGAGCTACCAAACTG GACTTACGTCTTACCATGGCA 20 21 55.0 47.6 58.3 57.8 0.0 13.0 1.526273 13584 16352 2895-2915 1.000000 68.5 psbM-petN_p1 psbM-petN 1 TGGAAGTCAATATTCTCGCA TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 54.5 58.2 0.0 0.0 3.670895 18702 19460 941-946 1.000000 51.1 psbM-petN_p2 psbM-petN 2 TGGAAGTCAATATTCTCGCA GTAGTATGGGGGAGGAGTGG 20 20 40.0 60.0 54.5 58.3 0.0 0.0 3.801267 18702 19460 885-890 1.000000 50.0 psbM-petN_p3 psbM-petN 3 TGGAAGTCAATATTCTCGCA TAGTATGGGGGAGGAGTGGA 20 20 40.0 55.0 54.5 58.4 0.0 0.0 3.891699 18702 19460 884-889 1.000000 49.3 psbM-petN_p4 psbM-petN 4 TGCTACTGCACTGTTTATTCT TAGTAAGTCTCGCTTGGGCT 21 20 38.1 50.0 55.2 58.2 0.0 0.0 3.908487 18702 19460 916-921 1.000000 49.2 psbM-petN_p5 psbM-petN 5 TGCTACTGCACTGTTTATTCT GTAGTATGGGGGAGGAGTGG 21 20 38.1 60.0 55.2 58.3 0.0 0.0 4.038859 18702 19460 860-865 1.000000 48.2 psaA_p1 psaA 1 AGCTAAGCCTTGGCTAAACC TTGGCGGGTCTCTTTGTATG 20 20 50.0 50.0 57.9 57.9 14.2 0.0 0.238222 41572 43824 2362-2362 1.000000 78.1 psaA_p2 psaA 2 GCTAAGCCTTGGCTAAACCT TTGGCGGGTCTCTTTGTATG 20 20 50.0 50.0 57.9 57.9 15.1 0.0 0.238222 41572 43824 2361-2361 1.000000 78.1 psaA_p3 psaA 3 AGCTAAGCCTTGGCTAAACC GTTGGCGGGTCTCTTTGTAT 20 20 50.0 50.0 57.9 57.9 14.2 0.0 0.239697 41572 43824 2363-2363 1.000000 78.1 psaA_p4 psaA 4 GCTAAGCCTTGGCTAAACCT GTTGGCGGGTCTCTTTGTAT 20 20 50.0 50.0 57.9 57.9 15.1 0.0 0.239697 41572 43824 2362-2362 1.000000 78.1 psaA_p5 psaA 5 AATACGACGAGTAGTGGGGT TTGGCGGGTCTCTTTGTATG 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.242739 41572 43824 2386-2386 1.000000 78.1 ycf3_p1 ycf3 1 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGA 20 20 45.0 45.0 57.8 55.7 0.0 21.9 2.453412 44453 46439 2130-2131 1.000000 60.4 ycf3_p2 ycf3 2 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGA 20 20 45.0 45.0 57.8 55.7 0.0 21.9 2.453412 44453 46439 2129-2130 1.000000 60.4 ycf3_p3 ycf3 3 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGAT 20 21 45.0 42.9 57.8 56.3 0.0 29.8 2.890713 44453 46439 2130-2131 1.000000 56.9 ycf3_p4 ycf3 4 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGAT 20 21 45.0 42.9 57.8 56.3 0.0 29.8 2.890713 44453 46439 2129-2130 1.000000 56.9 ycf3_p5 ycf3 5 GATCAACAACCAAACCACCC AGAGCATATGGATCGATCGA 20 20 50.0 45.0 57.2 55.7 0.0 21.9 3.098864 44453 46439 2131-2132 1.000000 55.3 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032006 48379 49201 924-925 1.000000 79.8 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073242 48379 49201 916-917 1.000000 79.5 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 48379 49201 894-895 1.000000 79.0 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 20 20 50.0 55.0 58.0 58.2 0.0 10.2 0.272624 48379 49201 914-915 1.000000 77.9 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 20 20 50.0 50.0 58.0 57.7 0.0 0.0 0.303758 48379 49201 923-924 1.000000 77.7 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 20 20 50.0 55.0 57.5 57.9 0.0 0.0 0.540775 52501 53274 880-907 1.000000 78.1 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 20 20 55.0 55.0 57.4 57.9 0.0 0.0 0.680025 52501 53274 884-911 1.000000 77.0 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 20 20 50.0 55.0 57.5 58.3 0.0 22.8 0.770156 52501 53274 881-908 1.000000 76.2 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TGGTCCTTCACTAATCGGGG GGTTCGAATCCGTATAGCCC 20 20 55.0 55.0 58.8 57.9 0.0 0.0 0.875460 52501 53274 883-910 1.000000 75.4 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CTGGTCCTTCACTAATCGGG CGGTTCGAATCCGTATAGCC 20 20 55.0 55.0 57.4 58.3 0.0 22.8 0.909407 52501 53274 885-912 1.000000 75.1 rbcL_p1 rbcL 1 AGTAGACCCTGTCGTTGTGA ACTGCATTTCGTAACTGCTT 20 20 50.0 40.0 58.0 56.0 0.0 9.9 2.035646 57004 58437 1565-1565 1.000000 63.7 rbcL_p2 rbcL 2 ATCGAGTAGACCCTGTCGTT ACTGCATTTCGTAACTGCTT 20 20 50.0 40.0 57.9 56.0 0.0 9.9 2.152540 57004 58437 1569-1569 1.000000 62.8 rbcL_p3 rbcL 3 AGTAGACCCTGTCGTTGTGA ACTGCATTTCGTAACTGCTTT 20 21 50.0 38.1 58.0 56.7 0.0 9.9 2.272339 57004 58437 1565-1565 1.000000 61.8 rbcL_p4 rbcL 4 TAGACCCTGTCGTTGTGAGA ACTGCATTTCGTAACTGCTT 20 20 50.0 40.0 57.7 56.0 0.0 9.9 2.308306 57004 58437 1563-1563 1.000000 61.5 rbcL_p5 rbcL 5 GTAGACCCTGTCGTTGTGAG ACTGCATTTCGTAACTGCTT 20 20 55.0 40.0 57.7 56.0 0.0 9.9 2.376064 57004 58437 1564-1564 1.000000 61.0 rbcL-psaI_p1 rbcL-psaI 1 GCCGCAGCTTGTGAAATATG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.2 58.0 0.0 26.9 0.195661 58438 59686 1388-1388 1.000000 78.8 rbcL-psaI_p2 rbcL-psaI 2 GCCGCAGCTTGTGAAATATG AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.2 57.9 0.0 26.8 0.272195 58438 59686 1394-1394 1.000000 78.2 rbcL-psaI_p3 rbcL-psaI 3 TCGAGTTCGATCCGGTAGAT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.7 58.0 0.0 26.9 0.278412 58438 59686 1354-1354 1.000000 78.2 rbcL-psaI_p4 rbcL-psaI 4 TCGAGTTCGATCCGGTAGAT AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 57.7 57.9 0.0 26.8 0.354946 58438 59686 1360-1360 1.000000 77.6 rbcL-psaI_p5 rbcL-psaI 5 GGCGATCAAATTCGAGTTCG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.6 58.0 0.0 26.9 0.380261 58438 59686 1365-1365 1.000000 77.4 petA_p1 petA 1 ATTCTGGGATCTGCGATTGG GCCAATAAATCGCGGCTTTT 20 20 50.0 45.0 57.7 58.1 0.0 6.1 0.345931 62035 62997 1030-1030 1.000000 77.3 petA_p2 petA 2 ATTCTGGGATCTGCGATTGG CAATCGCCAATAAATCGCGG 20 20 50.0 50.0 57.7 58.3 0.0 6.1 0.593414 62035 62997 1035-1035 1.000000 75.3 petA_p3 petA 3 ATTCTGGGATCTGCGATTGG AATCGCGGCTTTTTACCAAC 20 20 50.0 45.0 57.7 57.7 0.0 6.1 0.608492 62035 62997 1023-1023 1.000000 75.2 petA_p4 petA 4 TCTGGGATCTGCGATTGGAT GCCAATAAATCGCGGCTTTT 20 20 50.0 45.0 58.6 58.1 0.0 6.1 0.650948 62035 62997 1028-1028 1.000000 74.9 petA_p5 petA 5 ATTCTGGGATCTGCGATTGG AGCAATCGCCAATAAATCGC 20 20 50.0 45.0 57.7 57.6 0.0 0.0 0.673066 62035 62997 1037-1037 1.000000 74.7 psbE-petL_p1 psbE-petL 1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.493582 64751 65978 1386-1386 1.000000 76.2 psbE-petL_p2 psbE-petL 2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 20 20 45.0 50.0 58.0 58.5 0.0 0.0 0.497931 64751 65978 1384-1384 1.000000 76.1 psbE-petL_p3 psbE-petL 3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.527577 64751 65978 1375-1375 1.000000 75.9 psbE-petL_p4 psbE-petL 4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.9 58.5 6.4 0.0 0.531926 64751 65978 1373-1373 1.000000 75.9 psbE-petL_p5 psbE-petL 5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 58.2 58.5 0.0 0.0 0.713535 64751 65978 1377-1377 1.000000 74.4 infA_p1 infA 1 GTCTCTTCGCTTGAATTTTGGA ATGGTAATCGCCCCTCCTAT 22 20 40.9 50.0 57.3 57.7 0.0 0.0 3.093338 78815 79207 517-568 1.000000 59.3 infA_p2 infA 2 AGTCTCTTCGCTTGAATTTTGG ATGGTAATCGCCCCTCCTAT 22 20 40.9 50.0 57.2 57.7 0.0 0.0 3.100177 78815 79207 518-569 1.000000 59.2 infA_p3 infA 3 GTCTCTTCGCTTGAATTTTGGA CGCCCCTCCTATAGTACTCG 22 20 40.9 60.0 57.3 58.5 0.0 0.0 3.298178 78815 79207 509-560 1.000000 57.6 infA_p4 infA 4 AGTCTCTTCGCTTGAATTTTGG CGCCCCTCCTATAGTACTCG 22 20 40.9 60.0 57.2 58.5 0.0 0.0 3.305017 78815 79207 510-561 1.000000 57.6 infA_p5 infA 5 TCTCTTCGCTTGAATTTTGGAAC ATGGTAATCGCCCCTCCTAT 23 20 39.1 50.0 57.9 57.7 0.0 0.0 3.452477 78815 79207 516-567 1.000000 56.4 rps3_p1 rps3 1 ACAGAATGGGAAGACAAGGC TCCATCTTAGATTTCGATTCCCA 20 23 50.0 39.1 57.8 57.1 0.0 0.0 4.093791 82013 82732 792-793 1.000000 47.5 rps3_p2 rps3 2 ACAGAATGGGAAGACAAGGC TCCATCTTAGATTTCGATTCCCAG 20 24 50.0 41.7 57.8 58.1 0.0 0.0 4.348195 82013 82732 792-793 1.000000 45.4 rps3_p3 rps3 3 CAGAATGGGAAGACAAGGCT TCCATCTTAGATTTCGATTCCCA 20 23 50.0 39.1 57.5 57.1 0.0 0.0 4.391109 82013 82732 791-792 1.000000 45.1 rps3_p4 rps3 4 ACAGAATGGGAAGACAAGGC CCATCTTAGATTTCGATTCCCAG 20 23 50.0 43.5 57.8 56.8 0.0 0.0 4.395870 82013 82732 791-792 1.000000 45.0 rps3_p5 rps3 5 ACAGAATGGGAAGACAAGGC CCATCTTAGATTTCGATTCCCA 20 22 50.0 40.9 57.8 55.7 0.0 0.0 4.532421 82013 82732 791-792 1.000000 43.9 ndhF_p1 ndhF 1 GGAGAGATGGGGGAAGAAGA TGGTTTGGTGAATCGGAAACA 20 21 55.0 42.9 57.8 58.3 0.0 0.0 1.526513 105207 107426 2365-2365 1.000000 67.8 ndhF_p2 ndhF 2 AGGAAGGGGAATAAGAGGGG TGGTTTGGTGAATCGGAAACA 20 21 55.0 42.9 57.8 58.3 0.0 0.0 1.558362 105207 107426 2344-2344 1.000000 67.6 ndhF_p3 ndhF 3 GGAGAGATGGGGGAAGAAGA GGTTTGGTGAATCGGAAACA 20 20 55.0 45.0 57.8 56.5 0.0 0.0 1.638224 105207 107426 2364-2364 1.000000 66.9 ndhF_p4 ndhF 4 GGAGAGATGGGGGAAGAAGA TGGTTTGGTGAATCGGAAAC 20 20 55.0 45.0 57.8 56.5 0.0 0.0 1.638224 105207 107426 2365-2365 1.000000 66.9 ndhF_p5 ndhF 5 AGGAAGGGGAATAAGAGGGG GGTTTGGTGAATCGGAAACA 20 20 55.0 45.0 57.8 56.5 0.0 0.0 1.670073 105207 107426 2343-2343 1.000000 66.7 rps15-trnN-GUU_p1 rps15-trnN-GUU 1 CAGAACCCCTGTTTTCTTCT ACGACCAGTCAGTTAACAGC 20 20 45.0 50.0 55.2 57.8 0.0 8.6 2.982252 118504 120044 1663-1663 1.000000 56.2 rps15-trnN-GUU_p2 rps15-trnN-GUU 2 CAGAACCCCTGTTTTCTTCT AGGATTCGAACCTACGACCA 20 20 45.0 50.0 55.2 58.2 0.0 15.0 2.988487 118504 120044 1676-1676 1.000000 56.1 rps15-trnN-GUU_p3 rps15-trnN-GUU 3 CAGAACCCCTGTTTTCTTCT GGATTCGAACCTACGACCAG 20 20 45.0 55.0 55.2 57.8 0.0 0.0 3.037162 118504 120044 1675-1675 1.000000 55.8 rps15-trnN-GUU_p4 rps15-trnN-GUU 4 ACTTGAAATTCAACAGAACCCC ACGACCAGTCAGTTAACAGC 22 20 40.9 50.0 57.1 57.8 4.0 8.6 3.087598 118504 120044 1676-1676 1.000000 55.3 rps15-trnN-GUU_p5 rps15-trnN-GUU 5 ACTTGAAATTCAACAGAACCCC AGGATTCGAACCTACGACCA 22 20 40.9 50.0 57.1 58.2 4.0 15.0 3.093833 118504 120044 1689-1689 1.000000 55.3 trnR-ACG-rrn5_p1 trnR-ACG-rrn5 1 CTCAGGGGGAAAGTAAAGGC CACACCAATCCATCCCGAAT 20 20 55.0 50.0 57.9 57.9 0.0 0.0 0.187410 120448 120705 466-466 1.000000 78.5 trnR-ACG-rrn5_p2 trnR-ACG-rrn5 2 CTCAGGGGGAAAGTAAAGGC GGTGACGATACTGTAGGGGA 20 20 55.0 55.0 57.9 57.9 0.0 0.0 0.193243 120448 120705 425-425 1.000000 78.5 trnR-ACG-rrn5_p3 trnR-ACG-rrn5 3 CTCAGGGGGAAAGTAAAGGC ATCCATCCCGAATTTGGTGG 20 20 55.0 50.0 57.9 57.9 0.0 13.2 0.202050 120448 120705 459-459 1.000000 78.4 trnR-ACG-rrn5_p4 trnR-ACG-rrn5 4 GAAAGTAAAGGCCCTTCCCC CACACCAATCCATCCCGAAT 20 20 55.0 50.0 58.2 57.9 0.0 0.0 0.208486 120448 120705 458-458 1.000000 78.3 trnR-ACG-rrn5_p5 trnR-ACG-rrn5 5 GGAAAGTAAAGGCCCTTCCC CACACCAATCCATCCCGAAT 20 20 55.0 50.0 58.2 57.9 0.0 0.0 0.208486 120448 120705 459-459 1.000000 78.3