primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	rps2	1	TGAGATTCAGGAATAGTAACCGA	TCAATGGCCAATCCGCTTTA	23	20	39.1	45.0	56.6	57.9	0.0	11.0	4.582178	2796	3527	882-887	1.000000	44.5
rps2_p2	rps2	2	TGAGATTCAGGAATAGTAACCGA	GGGTTGTGTATCAATGGCCA	23	20	39.1	50.0	56.6	58.2	0.0	8.6	4.599873	2796	3527	892-897	1.000000	44.4
rps2_p3	rps2	3	TGAGATTCAGGAATAGTAACCGA	TGGCCAATCCGCTTTAGAAA	23	20	39.1	45.0	56.6	57.8	0.0	0.6	4.654012	2796	3527	878-883	1.000000	44.0
rps2_p4	rps2	4	TGAGATTCAGGAATAGTAACCGA	TATCAATGGCCAATCCGCTT	23	20	39.1	45.0	56.6	57.6	0.0	11.0	4.809186	2796	3527	884-889	1.000000	42.7
rps2_p5	rps2	5	TGAGATTCAGGAATAGTAACCGA	GTATCAATGGCCAATCCGCT	23	20	39.1	50.0	56.6	58.4	0.0	11.0	4.836672	2796	3527	885-890	1.000000	42.5
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	AGCTAACCAGACGCTCTACT	CGAGACTTTGGGTTATCCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.195843	23061	23211	336-336	1.000000	79.4
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	TTATGAGCCTTGCGAGCTAC	CGAGACTTTGGGTTATCCGG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.289136	23061	23211	274-274	1.000000	78.9
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	TATGAGCCTTGCGAGCTAAC	CGAGACTTTGGGTTATCCGG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.289136	23061	23211	349-349	1.000000	78.6
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	AGCTAACCAGACGCTCTACT	TGGTTCACGCATGTTTGTTG	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.332568	23061	23211	299-299	1.000000	78.4
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	TTATGAGCCTTGCGAGCTAC	TGGTTCACGCATGTTTGTTG	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.425861	23061	23211	237-237	1.000000	77.9
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	ATTTTCATTCCTTGCCCCGT	21	20	47.6	45.0	58.0	58.1	8.2	0.0	1.103293	30877	31675	937-938	1.000000	71.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	30877	31675	888-889	1.000000	71.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	30877	31675	909-910	1.000000	71.1
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	CATTCCTTGCCCCGTACTTT	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.206297	30877	31675	932-933	1.000000	71.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	TTTCATTCCTTGCCCCGTAC	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.211505	30877	31675	935-936	1.000000	70.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	33040	33567	601-601	1.000000	79.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	33040	33567	633-633	1.000000	79.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	33040	33567	589-589	1.000000	78.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	33040	33567	600-600	1.000000	77.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	33040	33567	621-621	1.000000	77.5
trnF-GAA-rbcL_p1	trnF-GAA-rbcL	1	GGGATAGCTCAGCTGGTAGA	CCAGCAGTAGATACCCTCGA	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.019845	34668	35770	1139-1212	1.000000	85.8
trnF-GAA-rbcL_p2	trnF-GAA-rbcL	2	CCTCGTGTCACCAGTTCAAA	CCAGCAGTAGATACCCTCGA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.069235	34668	35770	1103-1176	1.000000	85.4
trnF-GAA-rbcL_p3	trnF-GAA-rbcL	3	GGGATAGCTCAGCTGGTAGA	CGCAATGCTAGCAAATGGAG	20	20	55.0	50.0	58.0	57.9	10.3	23.5	0.117853	34668	35770	1211-1284	1.000000	84.9
trnF-GAA-rbcL_p4	trnF-GAA-rbcL	4	GGGATAGCTCAGCTGGTAGA	GCTGCTGCTAGTGAGGTATG	20	20	55.0	55.0	58.0	58.1	10.3	8.5	0.139238	34668	35770	1181-1254	1.000000	84.7
trnF-GAA-rbcL_p5	trnF-GAA-rbcL	5	CTGGTAGAGCAGAGGACTGA	CCAGCAGTAGATACCCTCGA	20	20	55.0	55.0	57.9	58.0	2.5	0.0	0.153638	34668	35770	1127-1200	1.000000	84.7
trnM-CAU-accD_p1	trnM-CAU-accD	1	TACGCCTTAACGACCAATGG	GCTGCTCTTCAATAGGACCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052520	40299	40822	1417-1486	1.000000	83.3
trnM-CAU-accD_p2	trnM-CAU-accD	2	TACGCCTTAACGACCAATGG	CTTGGTTCTTTGTCCAGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026054	40299	40822	1213-1213	0.333333	53.1
trnM-CAU-accD_p3	trnM-CAU-accD	3	TACGCCTTAACGACCAATGG	TCTTGGTTCTTTGTCCAGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031680	40299	40822	1214-1214	0.333333	53.1
trnM-CAU-accD_p4	trnM-CAU-accD	4	TACGCCTTAACGACCAATGG	CCAGTGTGGATTACGATGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035701	40299	40822	1167-1167	0.333333	53.0
trnM-CAU-accD_p5	trnM-CAU-accD	5	TACGCCTTAACGACCAATGG	ACCAGTGTGGATTACGATGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037112	40299	40822	1168-1168	0.333333	53.0
accD_p1	accD	1	GGGTCCTATTGAAGAGCAGC	GCCCTAGCCTCCCTAATCTT	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.303657	41182	41301	195-195	1.000000	85.6
accD_p2	accD	2	CACGGGTCCTATTGAAGAGC	GCCCTAGCCTCCCTAATCTT	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.322749	41182	41301	198-198	1.000000	85.4
accD_p3	accD	3	AGGAACTGGCTTCTGCAAAA	GCCCTAGCCTCCCTAATCTT	20	20	45.0	55.0	57.9	58.3	0.0	0.0	0.335852	41182	41301	238-238	1.000000	84.4
accD_p4	accD	4	AAGGTAAGGAGGAACTGGCT	GCCCTAGCCTCCCTAATCTT	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.310354	41182	41301	247-247	1.000000	84.3
accD_p5	accD	5	AGGTAAGGAGGAACTGGCTT	GCCCTAGCCTCCCTAATCTT	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.310354	41182	41301	246-246	1.000000	84.3
rps12-psbB_p1	rps12-psbB	1	ACACCCTAGTACACGTTCCT	GCCGGGATCATTCAATACGA	20	20	50.0	50.0	57.7	57.8	0.0	2.2	0.463144	54123	55204	1245-1246	1.000000	77.1
rps12-psbB_p2	rps12-psbB	2	ACACCCTAGTACACGTTCCT	TGGACAGAAATTAACCGGCC	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.470347	54123	55204	1262-1263	1.000000	77.0
rps12-psbB_p3	rps12-psbB	3	GCGGGGGATTTCTTGACATT	GCCGGGATCATTCAATACGA	20	20	50.0	50.0	58.5	57.8	0.0	2.2	0.689488	54123	55204	1201-1202	1.000000	75.3
rps12-psbB_p4	rps12-psbB	4	GCGGGGGATTTCTTGACATT	TGGACAGAAATTAACCGGCC	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.696691	54123	55204	1218-1219	1.000000	75.2
rps12-psbB_p5	rps12-psbB	5	ACCCTAGTACACGTTCCTCG	GCCGGGATCATTCAATACGA	20	20	55.0	50.0	58.5	57.8	0.0	2.2	0.706472	54123	55204	1243-1244	1.000000	75.1
rpl22_p1	rpl22	1	GTTGTGCAAACCAAAGGGAA	AAGTCAAGGGACCATTTGGA	20	20	45.0	45.0	57.3	56.6	0.0	3.0	2.051673	66020	66562	653-653	1.000000	66.2
rpl22_p2	rpl22	2	GTTGTGCAAACCAAAGGGAA	AGTCAAGGGACCATTTGGAT	20	20	45.0	45.0	57.3	56.4	0.0	3.0	2.288577	66020	66562	652-652	1.000000	64.3
rpl22_p3	rpl22	3	GTTGTGCAAACCAAAGGGAA	AAAGTCAAGGGACCATTTGGA	20	21	45.0	42.9	57.3	57.4	0.0	3.0	2.293180	66020	66562	654-654	1.000000	64.2
rpl22_p4	rpl22	4	GTTGTGCAAACCAAAGGGAA	GACCATAAAGTCAAGGGACCA	20	21	45.0	47.6	57.3	57.3	0.0	0.0	2.395981	66020	66562	660-660	1.000000	63.4
rpl22_p5	rpl22	5	GTTGTGCAAACCAAAGGGAA	TGACCATAAAGTCAAGGGACC	20	21	45.0	47.6	57.3	57.3	0.0	0.0	2.395981	66020	66562	661-661	1.000000	63.4
atpF-atpA_p1	atpF-atpA	1	TGACATGTTTGGTGCCATGA	CAATGCCGTCACCTACTTGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.100769	68821	68888	223-223	1.000000	80.6
atpF-atpA_p2	atpF-atpA	2	TGACATGTTTGGTGCCATGA	TGCTAATTTCGTCTGCTCGA	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.654992	68821	68888	136-136	1.000000	77.1
atpF-atpA_p3	atpF-atpA	3	TGACATGTTTGGTGCCATGA	AATGCCGTCACCTACTTGAA	20	20	45.0	45.0	58.0	57.1	0.0	0.0	0.858725	68821	68888	222-222	1.000000	74.6
atpF-atpA_p4	atpF-atpA	4	TGACATGTTTGGTGCCATGA	ATGCCGTCACCTACTTGAAA	20	20	45.0	45.0	58.0	57.1	0.0	0.0	0.858725	68821	68888	221-221	1.000000	74.6
atpF-atpA_p5	atpF-atpA	5	TGACATGTTTGGTGCCATGA	TGCCGTCACCTACTTGAAAT	20	20	45.0	45.0	58.0	57.1	0.0	0.0	0.858725	68821	68888	220-220	1.000000	74.6
ycf1_p1	ycf1	1	ACCGCAAAAGATCCTTCTCC	GTAGTTTGTATGGCCAGCGA	20	20	50.0	50.0	57.9	58.0	0.0	13.0	0.133885	89794	89913	1081-1102	1.000000	82.5
ycf1_p2	ycf1	2	ACCGCAAAAGATCCTTCTCC	AGTAGTTTGTATGGCCAGCG	20	20	50.0	50.0	57.9	58.0	0.0	13.0	0.139518	89794	89913	1082-1103	1.000000	82.4
ycf1_p3	ycf1	3	AGAGGGATTCACCGCAAAAG	GTAGTTTGTATGGCCAGCGA	20	20	50.0	50.0	58.2	58.0	0.0	13.0	0.192157	89794	89913	1091-1112	1.000000	82.0
ycf1_p4	ycf1	4	GAAGAGGGATTCACCGCAAA	GTAGTTTGTATGGCCAGCGA	20	20	50.0	50.0	58.2	58.0	0.0	13.0	0.197325	89794	89913	1093-1114	1.000000	81.9
ycf1_p5	ycf1	5	GAGGGATTCACCGCAAAAGA	GTAGTTTGTATGGCCAGCGA	20	20	50.0	50.0	58.2	58.0	0.0	13.0	0.197325	89794	89913	1090-1111	1.000000	81.9
rpl32_p1	rpl32	1	AACAAAAACGTTGATGGGGA	TCTTTCACATCCAACTACACCA	20	22	40.0	40.9	56.1	57.3	15.5	0.0	4.642422	98961	99134	248-248	1.000000	45.8
rpl32_p2	rpl32	2	ACAAAAACGTTGATGGGGAA	TCTTTCACATCCAACTACACCA	20	22	40.0	40.9	56.1	57.3	15.5	0.0	4.642422	98961	99134	247-247	1.000000	45.8
rpl32_p3	rpl32	3	AACAAAAACGTTGATGGGGA	TGCATAAAGAGGGTATCGTCT	20	21	40.0	42.9	56.1	56.2	15.5	0.0	4.697314	98961	99134	326-326	1.000000	44.6
rpl32_p4	rpl32	4	ACAAAAACGTTGATGGGGAA	TGCATAAAGAGGGTATCGTCT	20	21	40.0	42.9	56.1	56.2	15.5	0.0	4.697314	98961	99134	325-325	1.000000	44.6
rpl32_p5	rpl32	5	ACAAAAACGTTGATGGGGAAA	TCTTTCACATCCAACTACACCA	21	22	38.1	40.9	56.8	57.3	15.5	0.0	4.872285	98961	99134	247-247	1.000000	43.9
trnA-UGC_p1	trnA-UGC	1	AGACATCCAACCCGTAATCG	TCATACATGCTCCACTTGGC	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.639590	104384	104420	92-93	1.000000	78.3
trnA-UGC_p2	trnA-UGC	2	AGACATCCAACCCGTAATCG	CATACATGCTCCACTTGGCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.645348	104384	104420	91-92	1.000000	78.3
trnA-UGC_p3	trnA-UGC	3	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.601486	104384	104420	186-187	1.000000	76.9
trnA-UGC_p4	trnA-UGC	4	ATCCAACCCGTAATCGCAAC	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.6	58.0	0.0	0.0	0.629032	104384	104420	182-183	1.000000	76.7
trnA-UGC_p5	trnA-UGC	5	CATCCAACCCGTAATCGCAA	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.6	58.0	0.0	0.0	0.630023	104384	104420	183-184	1.000000	76.7
atpA-atpF_p1	atpA-atpF	1	CAATGCCGTCACCTACTTGA	TGACATGTTTGGTGCCATGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.100769	132599	132666	223-223	1.000000	80.6
atpA-atpF_p2	atpA-atpF	2	TGCTAATTTCGTCTGCTCGA	TGACATGTTTGGTGCCATGA	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.654992	132599	132666	136-136	1.000000	77.1
atpA-atpF_p3	atpA-atpF	3	AATGCCGTCACCTACTTGAA	TGACATGTTTGGTGCCATGA	20	20	45.0	45.0	57.1	58.0	0.0	0.0	0.858725	132599	132666	222-222	1.000000	74.6
atpA-atpF_p4	atpA-atpF	4	ATGCCGTCACCTACTTGAAA	TGACATGTTTGGTGCCATGA	20	20	45.0	45.0	57.1	58.0	0.0	0.0	0.858725	132599	132666	221-221	1.000000	74.6
atpA-atpF_p5	atpA-atpF	5	TGCCGTCACCTACTTGAAAT	TGACATGTTTGGTGCCATGA	20	20	45.0	45.0	57.1	58.0	0.0	0.0	0.858725	132599	132666	220-220	1.000000	74.6