primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GGAGCAATAACCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	56.9	58.0	0.0	0.0	1.120585	310	1368	1207-1207	1.000000	71.0
psbA_p2	psbA	2	AGGAGCAATAACCCCTCTTG	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	56.9	58.0	0.0	0.0	1.129230	310	1368	1208-1208	1.000000	71.0
psbA_p3	psbA	3	AAGGAGCAATAACCCCTCTTG	AAATTCGTGTGCTTGGGAGT	21	20	47.6	45.0	57.6	58.0	0.0	0.0	1.391184	310	1368	1209-1209	1.000000	68.9
psbA_p4	psbA	4	AGGAGCAATAACCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	21	20	47.6	45.0	58.4	58.0	0.0	0.0	1.456448	310	1368	1208-1208	1.000000	68.3
psbA_p5	psbA	5	GGAGCAATAACCCCTCTTGA	AATTCGTGTGCTTGGGAGTC	20	20	50.0	50.0	56.9	58.5	0.0	0.0	1.578084	310	1368	1206-1206	1.000000	67.4
rps16_p1	rps16	1	TCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	21	21	47.6	42.9	56.5	57.5	1.8	0.0	3.966559	4911	6078	1279-1279	1.000000	48.3
rps16_p2	rps16	2	TTCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	22	21	45.5	42.9	57.2	57.5	1.8	0.0	4.244669	4911	6078	1280-1280	1.000000	46.1
rps16_p3	rps16	3	TCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGAC	21	20	47.6	45.0	56.5	56.0	1.8	0.0	4.492972	4911	6078	1279-1279	1.000000	44.1
rps16_p4	rps16	4	TCTATACAAGTTGCTACCCCC	ATGTACAACCGAACCAATGACT	21	22	47.6	40.9	56.5	58.0	1.8	0.0	4.503575	4911	6078	1280-1280	1.000000	44.0
rps16_p5	rps16	5	CTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	20	21	50.0	42.9	54.9	57.5	1.8	0.0	4.558768	4911	6078	1278-1278	1.000000	43.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	11.1	16.9	0.189961	6079	6816	832-832	1.000000	78.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCACAACGGATCATGTCCTT	CGAAATTGAAATGGGGCGTC	20	20	50.0	50.0	57.9	58.1	11.1	0.0	0.205795	6079	6816	891-891	1.000000	78.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCACAACGGATCATGTCCTT	GAAATTGAAATGGGGCGTCG	20	20	50.0	50.0	57.9	58.1	11.1	0.0	0.205795	6079	6816	890-890	1.000000	78.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6079	6816	811-811	1.000000	78.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTCG	20	20	45.0	50.0	58.1	58.1	8.1	0.0	0.231259	6079	6816	869-869	1.000000	78.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	7912	8033	234-235	1.000000	79.8
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	7912	8033	235-236	1.000000	79.8
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	7912	8033	235-236	1.000000	79.8
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	7912	8033	236-237	1.000000	79.8
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176426	7912	8033	253-254	1.000000	78.9
atpF_p1	atpF	1	GGCTCGAATGGTTACCATGA	AAGAGGGGCAAAGTAATAGA	20	20	50.0	40.0	57.7	53.3	18.5	0.0	5.035495	11539	12789	1397-1399	1.000000	40.1
atpF_p2	atpF	2	GGCTCGAATGGTTACCATGA	AGAGGGGCAAAGTAATAGAA	20	20	50.0	40.0	57.7	53.3	18.5	0.0	5.035495	11539	12789	1396-1398	1.000000	40.1
atpF_p3	atpF	3	GGCTCGAATGGTTACCATGA	AAAGAGGGGCAAAGTAATAGA	20	21	50.0	38.1	57.7	54.2	18.5	0.0	5.114034	11539	12789	1398-1400	1.000000	40.1
atpF_p4	atpF	4	GGCTCGAATGGTTACCATGA	AAGAGGGGCAAAGTAATAGAA	20	21	50.0	38.1	57.7	54.2	18.5	0.0	5.114034	11539	12789	1397-1399	1.000000	40.1
atpF_p5	atpF	5	GGCTCGAATGGTTACCATGA	AGAGGGGCAAAGTAATAGAAA	20	21	50.0	38.1	57.7	54.2	18.5	0.0	5.114034	11539	12789	1396-1398	1.000000	40.1
ycf3_p1	ycf3	1	ACAAAGAGACCTGCCAACAG	TCGGAATAGTTGCCTTCGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.782074	41704	43657	2915-2943	1.000000	73.8
ycf3_p2	ycf3	2	CAAAGAGACCTGCCAACAGT	TCGGAATAGTTGCCTTCGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.782074	41704	43657	2914-2942	1.000000	73.8
ycf3_p3	ycf3	3	AATTAAAAATCACGCGCGCA	TCGGAATAGTTGCCTTCGTT	20	20	40.0	45.0	58.0	57.2	32.6	0.0	0.784500	41704	43657	2626-2654	1.000000	73.8
ycf3_p4	ycf3	4	TTAAAAATCACGCGCGCATT	TCGGAATAGTTGCCTTCGTT	20	20	40.0	45.0	58.0	57.2	32.6	0.0	0.784500	41704	43657	2624-2652	1.000000	73.8
ycf3_p5	ycf3	5	GAGACCTGCCAACAGTCAAA	TCGGAATAGTTGCCTTCGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.787628	41704	43657	2910-2938	1.000000	73.7
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTGATTTTGGCAC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.489957	62749	63771	1132-1132	1.000000	76.2
petA-psbJ_p2	petA-psbJ	2	GGCATCTGTGATTTTGGCAC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.0	57.4	0.0	0.0	0.551259	62749	63771	1131-1131	1.000000	75.7
petA-psbJ_p3	petA-psbJ	3	TGGCATCTGTGATTTTGGCA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	58.4	57.5	0.0	0.0	0.859305	62749	63771	1133-1133	1.000000	73.2
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.6	57.5	0.0	0.0	0.902837	62749	63771	1159-1159	1.000000	72.8
petA-psbJ_p5	petA-psbJ	5	TGGCATCTGTGATTTTGGCA	CCGGATTAGGTTCATCCCTG	20	20	45.0	55.0	58.4	57.4	0.0	0.0	0.920607	62749	63771	1132-1132	1.000000	72.7
rps18-rpl20_p1	rps18-rpl20	1	AACCGAGTCGACCACTAGAA	CCAACGAAATCCGAAAAGAAGT	20	22	50.0	40.9	58.1	57.5	15.6	0.0	2.581886	67928	68167	341-342	1.000000	59.6
rps18-rpl20_p2	rps18-rpl20	2	AAGAACCGAGTCGACCACTA	CCAACGAAATCCGAAAAGAAGT	20	22	50.0	40.9	58.1	57.5	15.6	0.0	2.581886	67928	68167	344-345	1.000000	59.6
rps18-rpl20_p3	rps18-rpl20	3	TTGAAAGAACCGAGTCGACC	CCAACGAAATCCGAAAAGAAGT	20	22	50.0	40.9	57.9	57.5	15.6	0.0	2.627958	67928	68167	348-349	1.000000	59.2
rps18-rpl20_p4	rps18-rpl20	4	AACCGAGTCGACCACTAGAA	TCCAACGAAATCCGAAAAGA	20	20	50.0	40.0	58.1	55.3	15.6	0.0	2.788629	67928	68167	342-343	1.000000	57.9
rps18-rpl20_p5	rps18-rpl20	5	AAGAACCGAGTCGACCACTA	TCCAACGAAATCCGAAAAGA	20	20	50.0	40.0	58.1	55.3	15.6	0.0	2.788629	67928	68167	345-346	1.000000	57.9
clpP1_p1	clpP1	1	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGA	21	20	47.6	40.0	57.7	55.5	0.0	0.0	3.778547	69634	71614	2137-2138	1.000000	49.8
clpP1_p2	clpP1	2	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAA	21	21	47.6	38.1	57.7	56.3	0.0	0.0	3.989701	69634	71614	2137-2138	1.000000	48.1
clpP1_p3	clpP1	3	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGA	21	20	47.6	40.0	57.4	55.5	0.0	0.0	4.066145	69634	71614	2136-2137	1.000000	47.5
clpP1_p4	clpP1	4	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAAA	21	22	47.6	36.4	57.7	57.0	0.0	0.0	4.271502	69634	71614	2137-2138	1.000000	45.9
clpP1_p5	clpP1	5	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGAA	21	21	47.6	38.1	57.4	56.3	0.0	0.0	4.277299	69634	71614	2136-2137	1.000000	45.8
rpl22-rps19_p1	rpl22-rps19	1	AATTTCTTCGCCGTAGTCCC	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.114310	83377	83438	895-896	1.000000	79.4
rpl22-rps19_p2	rpl22-rps19	2	CGCATAACTACGTCCTCGAG	AAAAGTTTGGGTAGAGCCGG	20	20	55.0	50.0	58.0	58.1	5.1	0.0	0.114442	83377	83438	926-927	1.000000	79.4
rpl22-rps19_p3	rpl22-rps19	3	AATTTCTTCGCCGTAGTCCC	ATTACCTTCTGGGGAGGTCC	20	20	50.0	55.0	58.0	58.1	0.0	0.1	0.125248	83377	83438	977-978	1.000000	79.3
rpl22-rps19_p4	rpl22-rps19	4	CGCATAACTACGTCCTCGAG	ATTACCTTCTGGGGAGGTCC	20	20	55.0	55.0	58.0	58.1	5.1	0.1	0.125380	83377	83438	1008-1009	1.000000	79.3
rpl22-rps19_p5	rpl22-rps19	5	AATTTCTTCGCCGTAGTCCC	TGGGGAGGTCCGTTTGATAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132150	83377	83438	968-969	1.000000	79.3
rps19_p1	rps19	1	CGACCTAAAGCGTATACTTCTGT	GATTGTTCGTCGCCGTAGTA	23	20	43.5	50.0	58.1	57.8	0.6	0.0	3.260437	83439	83717	464-465	1.000000	54.6
rps19_p2	rps19	2	CGACCTAAAGCGTATACTTCTGT	TTTGATTGTTCGTCGCCGTA	23	20	43.5	45.0	58.1	58.2	0.6	0.0	3.301157	83439	83717	467-468	1.000000	54.3
rps19_p3	rps19	3	CGACCTAAAGCGTATACTTCTGT	AATTTGATTGTTCGTCGCCG	23	20	43.5	45.0	58.1	57.8	0.6	0.0	3.323222	83439	83717	469-470	1.000000	54.1
rps19_p4	rps19	4	TCGACCTAAAGCGTATACTTCTG	GATTGTTCGTCGCCGTAGTA	23	20	43.5	50.0	57.8	57.8	0.6	0.0	3.344385	83439	83717	465-466	1.000000	53.9
rps19_p5	rps19	5	TCGACCTAAAGCGTATACTTCTG	TTTGATTGTTCGTCGCCGTA	23	20	43.5	45.0	57.8	58.2	0.6	0.0	3.385105	83439	83717	468-469	1.000000	53.6
ycf2_p1	ycf2	1	TCCACAACAACGAAAGCACT	CCCATGATCCTGAACCGATC	20	20	45.0	55.0	58.3	57.8	0.0	0.0	0.430646	91340	91459	259-259	1.000000	76.9
ycf2_p2	ycf2	2	TCCACAACAACGAAAGCACT	GATCCCATGATCCTGAACCG	20	20	45.0	55.0	58.3	57.8	0.0	0.0	0.430646	91340	91459	262-262	1.000000	76.9
ycf2_p3	ycf2	3	TCCACAACAACGAAAGCACT	ATCCCATGATCCTGAACCGA	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.448272	91340	91459	261-261	1.000000	76.7
ycf2_p4	ycf2	4	TCCACAACAACGAAAGCACT	TCCCATGATCCTGAACCGAT	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.448272	91340	91459	260-260	1.000000	76.7
ycf2_p5	ycf2	5	TCCACAACAACGAAAGCACT	TGAACCGATCTTACCTGGGA	20	20	45.0	50.0	58.3	57.8	0.0	0.0	0.496049	91340	91459	249-249	1.000000	76.4
ccsA-ndhD_p1	ccsA-ndhD	1	TTTTTACCCAAGGCTTCGCT	TCAGTAGCTTTTCGATGGCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.034469	114587	114831	1269-1269	1.000000	79.8
ccsA-ndhD_p2	ccsA-ndhD	2	TTTTTACCCAAGGCTTCGCT	CAGTAGCTTTTCGATGGCCT	20	20	45.0	50.0	58.0	58.0	0.0	3.3	0.040132	114587	114831	1268-1268	1.000000	79.7
ccsA-ndhD_p3	ccsA-ndhD	3	AATTCAGCAATTGTGGCGTG	TCAGTAGCTTTTCGATGGCC	20	20	45.0	50.0	57.9	58.0	7.4	0.0	0.093013	114587	114831	733-733	1.000000	79.4
ccsA-ndhD_p4	ccsA-ndhD	4	AATTCAGCAATTGTGGCGTG	CAGTAGCTTTTCGATGGCCT	20	20	45.0	50.0	57.9	58.0	7.4	3.3	0.098675	114587	114831	732-732	1.000000	79.3
ccsA-ndhD_p5	ccsA-ndhD	5	GGAAGGGTCCAAATTCAGCA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.100820	114587	114831	744-744	1.000000	79.3
ndhD_p1	ndhD	1	ACGTAGGAAAAGAAGAAAAAGCC	TTACCGACAAAATCCGTGCT	23	20	39.1	45.0	57.6	57.8	0.0	0.0	3.607203	114832	116361	1681-1681	1.000000	51.2
ndhD_p2	ndhD	2	ACGTAGGAAAAGAAGAAAAAGCC	TACCGACAAAATCCGTGCTC	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.717158	114832	116361	1680-1680	1.000000	50.3
ndhD_p3	ndhD	3	CGTAGGAAAAGAAGAAAAAGCCT	TTACCGACAAAATCCGTGCT	23	20	39.1	45.0	57.3	57.8	0.0	0.0	3.863848	114832	116361	1680-1680	1.000000	49.1
ndhD_p4	ndhD	4	CGTAGGAAAAGAAGAAAAAGCCT	TACCGACAAAATCCGTGCTC	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.973803	114832	116361	1679-1679	1.000000	48.3
ndhD_p5	ndhD	5	ACGTAGGAAAAGAAGAAAAAGCC	CGACAAAATCCGTGCTCAAA	23	20	39.1	45.0	57.6	57.3	0.0	0.0	4.113706	114832	116361	1677-1677	1.000000	47.1
ycf1_p1	ycf1	1	TCAAGTGTAGTGGGGGATAGT	CCCCTTTTGTCTCTTCTTTTCG	21	22	47.6	45.5	57.5	57.5	0.0	0.0	3.974632	127317	127436	218-218	1.000000	48.6
ycf1_p2	ycf1	2	AGTGTAGTGGGGGATAGTCT	CCCCTTTTGTCTCTTCTTTTCG	20	22	50.0	45.5	56.4	57.5	0.0	0.0	4.042678	127317	127436	215-215	1.000000	48.0
ycf1_p3	ycf1	3	TTTTCAAGTGTAGTGGGGGA	CCCCTTTTGTCTCTTCTTTTCG	20	22	45.0	45.5	56.3	57.5	0.0	0.0	4.219277	127317	127436	221-221	1.000000	46.6
ycf1_p4	ycf1	4	AAGTGTAGTGGGGGATAGTCT	CCCCTTTTGTCTCTTCTTTTCG	21	22	47.6	45.5	57.2	57.5	0.0	0.0	4.268301	127317	127436	216-216	1.000000	46.2
ycf1_p5	ycf1	5	AGTGTAGTGGGGGATAGTCTT	CCCCTTTTGTCTCTTCTTTTCG	21	22	47.6	45.5	57.2	57.5	0.0	0.0	4.268301	127317	127436	215-215	1.000000	46.2
ycf2_p1	ycf2	1	ATGCTCGTTCCAAGTTCGAA	TGCACGAGATCTTGTAGCAC	20	20	45.0	50.0	57.8	58.0	0.0	24.5	0.167526	145304	145423	303-303	1.000000	78.9
ycf2_p2	ycf2	2	ATGCTCGTTCCAAGTTCGAA	GGTTCCAATGCACGAGATCT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.193656	145304	145423	311-311	1.000000	78.7
ycf2_p3	ycf2	3	ATGCTCGTTCCAAGTTCGAA	TACTAACGGATTCGGGTCCA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.306174	145304	145423	339-339	1.000000	77.8
ycf2_p4	ycf2	4	ATGCTCGTTCCAAGTTCGAA	ACCAATGAGGCCCTATCGAT	20	20	45.0	50.0	57.8	58.3	0.0	2.2	0.429163	145304	145423	281-281	1.000000	76.9
ycf2_p5	ycf2	5	ATGCTCGTTCCAAGTTCGAA	GCACGAGATCTTGTAGCACT	20	20	45.0	50.0	57.8	57.7	0.0	22.4	0.449645	145304	145423	302-302	1.000000	76.7