primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbT-psbN_p1 psbT-psbN 1 TTTTCGAGAACCACCCAAGG TCGCCATCTCCATATCTGGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.039797 78062 78127 222-222 1.000000 82.6 psbT-psbN_p2 psbT-psbN 2 TTTTCGAGAACCACCCAAGG CCCTAGTCGCCATCTCCATA 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.099609 78062 78127 228-228 1.000000 82.0 psbT-psbN_p3 psbT-psbN 3 TTTTCGAGAACCACCCAAGG GATCCATTCGAGGAACACGG 20 20 50.0 55.0 58.0 58.4 0.0 0.0 0.452422 78062 78127 136-136 1.000000 81.1 psbT-psbN_p4 psbT-psbN 4 TTTTCGAGAACCACCCAAGG GGGTACGCCTTATATACCGC 20 20 50.0 55.0 58.0 57.7 0.0 0.0 0.308603 78062 78127 184-184 1.000000 81.0 psbT-psbN_p5 psbT-psbN 5 TTTTCGAGAACCACCCAAGG GTCGCCATCTCCATATCTGG 20 20 50.0 55.0 58.0 57.6 0.0 0.0 0.401148 78062 78127 223-223 1.000000 79.7 ycf1_p1 ycf1 1 AAGAGGGATCCACCGAAGAA CGGTTCATTCCCCATCCAAT 20 20 50.0 50.0 58.0 57.9 15.2 0.0 0.119156 114507 114626 1508-1508 0.500000 59.0 ycf1_p2 ycf1 2 TTGCACACATGTCCAAGTGA CGGTTCATTCCCCATCCAAT 20 20 45.0 50.0 58.0 57.9 16.0 0.0 0.121666 114507 114626 1170-1170 0.500000 59.0 ycf1_p3 ycf1 3 AAGAGGGATCCACCGAAGAA CACCTCTTCTGCTCGATCAC 20 20 50.0 55.0 58.0 58.1 15.2 0.0 0.127460 114507 114626 1289-1289 0.500000 59.0 ycf1_p4 ycf1 4 AAGAGGGATCCACCGAAGAA CCGTTCATCCTCGTCTTGTT 20 20 50.0 50.0 58.0 57.9 15.2 0.0 0.127665 114507 114626 1592-1592 0.500000 59.0 ycf1_p5 ycf1 5 TTGCACACATGTCCAAGTGA CACCTCTTCTGCTCGATCAC 20 20 45.0 55.0 58.0 58.1 16.0 0.0 0.129970 114507 114626 951-951 0.500000 59.0 rps15_p1 rps15 1 CCAGATCGAAATGACTGGCA GAAAGGAGTCTGTGCACCAA 20 20 50.0 50.0 58.0 58.0 0.0 15.7 0.061101 118928 119191 850-850 0.500000 59.5 rps15_p2 rps15 2 CCAGATCGAAATGACTGGCA TTCCTCTCCGCCAATAATGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.061101 118928 119191 1096-1096 0.500000 59.5 rps15_p3 rps15 3 ACCAGATCGAAATGACTGGC GAAAGGAGTCTGTGCACCAA 20 20 50.0 50.0 58.0 58.0 18.6 15.7 0.062520 118928 119191 851-851 0.500000 59.5 rps15_p4 rps15 4 ACCAGATCGAAATGACTGGC TTCCTCTCCGCCAATAATGC 20 20 50.0 50.0 58.0 58.0 18.6 0.0 0.062520 118928 119191 1097-1097 0.500000 59.5 rps15_p5 rps15 5 CCAGATCGAAATGACTGGCA AACACCATGCATTGATGGGT 20 20 50.0 45.0 58.0 58.1 0.0 8.9 0.087043 118928 119191 532-532 0.500000 59.3 ndhH_p1 ndhH 1 GAAAACGTCAACGACTGCTG TACGTTGTTGTATCCCTGCG 20 20 50.0 50.0 58.0 58.0 9.9 0.0 0.006140 119296 120483 1578-1578 0.500000 60.0 ndhH_p2 ndhH 2 CCAGATCGAAATGACTGGCA TACGTTGTTGTATCCCTGCG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.029372 119296 120483 1824-1824 0.500000 59.8 ndhH_p3 ndhH 3 ACCAGATCGAAATGACTGGC TACGTTGTTGTATCCCTGCG 20 20 50.0 50.0 58.0 58.0 18.6 0.0 0.030790 119296 120483 1825-1825 0.500000 59.8 ndhH_p4 ndhH 4 GAAAACGTCAACGACTGCTG TGTTCCAACGAATCACACGT 20 20 50.0 45.0 58.0 58.1 9.9 0.0 0.062511 119296 120483 1965-1965 0.500000 59.5 ndhH_p5 ndhH 5 CCAGATCGAAATGACTGGCA TGTTCCAACGAATCACACGT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.085742 119296 120483 2211-2211 0.500000 59.3 ndhA_p1 ndhA 1 ATGGAGATGGAAAATCCGCC TTGGACAATACTCGACGCAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.022318 120485 122665 2747-2747 0.500000 59.8 ndhA_p2 ndhA 2 CAGGCTCTGGAAGGAATTCC TTGGACAATACTCGACGCAG 20 20 55.0 50.0 58.0 58.0 17.3 0.0 0.059089 120485 122665 2965-2965 0.500000 59.5 ndhA_p3 ndhA 3 ATGGAGATGGAAAATCCGCC TGCAGCTCGTACTGTTTGTT 20 20 50.0 45.0 58.0 58.1 0.0 8.8 0.065013 120485 122665 2458-2458 0.500000 59.5 ndhA_p4 ndhA 4 ATGGAGATGGAAAATCCGCC TTGCAGCTCGTACTGTTTGT 20 20 50.0 45.0 58.0 58.1 0.0 8.8 0.065013 120485 122665 2459-2459 0.500000 59.5 ndhA_p5 ndhA 5 ATGGAGATGGAAAATCCGCC AACCCAGTCACCATAGGGAA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.066864 120485 122665 2424-2424 0.500000 59.5 ndhI_p1 ndhI 1 CATCGAGGAGCCGTATGAAG GACATTTATGGCTCCCACGT 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.095890 122734 123291 1807-1807 0.500000 59.2 ndhI_p2 ndhI 2 GCGGGTGGAATTTCCCTATT GACATTTATGGCTCCCACGT 20 20 50.0 50.0 57.9 58.0 12.9 0.0 0.105752 122734 123291 1448-1448 0.500000 59.2 ndhI_p3 ndhI 3 CATCGAGGAGCCGTATGAAG CATGACCTTACTTACCGGGC 20 20 55.0 55.0 57.9 58.1 0.0 6.3 0.108038 122734 123291 1554-1554 0.500000 59.1 ndhI_p4 ndhI 4 ACCCTACAAACATGCCAAGG GACATTTATGGCTCCCACGT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.115031 122734 123291 1861-1861 0.500000 59.1 ndhI_p5 ndhI 5 CCCTACAAACATGCCAAGGT GACATTTATGGCTCCCACGT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.115031 122734 123291 1860-1860 0.500000 59.1 ndhG_p1 ndhG 1 AACTGCGTCGAGTATTGTCC ACAATAGCTGGCCCAATAGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.020948 123635 124165 1591-1591 0.500000 59.8 ndhG_p2 ndhG 2 CTGCGTCGAGTATTGTCCAA ACAATAGCTGGCCCAATAGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.022318 123635 124165 1589-1589 0.500000 59.8 ndhG_p3 ndhG 3 AACTGCGTCGAGTATTGTCC CCAACTCCCCATCAATCTCG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.048789 123635 124165 1346-1346 0.500000 59.6 ndhG_p4 ndhG 4 CTGCGTCGAGTATTGTCCAA CCAACTCCCCATCAATCTCG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.050159 123635 124165 1344-1344 0.500000 59.6 ndhG_p5 ndhG 5 GCCCGGTAAGTAAGGTCATG ACAATAGCTGGCCCAATAGC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.063652 123635 124165 1086-1086 0.500000 59.5 ndhG-ndhE_p1 ndhG-ndhE 1 ACGTGGGAGCCATAAATGTC ACAATAGCTGGCCCAATAGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.051504 124166 124424 833-833 0.500000 59.6 ndhG-ndhE_p2 ndhG-ndhE 2 GCCCGGTAAGTAAGGTCATG ACAATAGCTGGCCCAATAGC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.063652 124166 124424 1086-1086 0.500000 59.5 ndhG-ndhE_p3 ndhG-ndhE 3 ACGTGGGAGCCATAAATGTC ACCATGTTTCGGCTTGTGAT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.128687 124166 124424 674-674 0.500000 59.0 ndhG-ndhE_p4 ndhG-ndhE 4 GGATACAGAAGCGGGATTGG ACAATAGCTGGCCCAATAGC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.128882 124166 124424 1209-1209 0.500000 59.0 ndhG-ndhE_p5 ndhG-ndhE 5 ACGTGGGAGCCATAAATGTC GTCCTCTGTTCTTGGAGCAG 20 20 50.0 55.0 58.0 57.9 0.0 5.3 0.130239 124166 124424 1281-1281 0.500000 59.0 ndhE_p1 ndhE 1 ACGTGGGAGCCATAAATGTC CTGCGAGTTGTCTCATTCGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055775 124425 124727 1367-1367 0.500000 59.6 ndhE_p2 ndhE 2 CGAGATTGATGGGGAGTTGG CTGCGAGTTGTCTCATTCGA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.058346 124425 124727 799-799 0.500000 59.5 ndhE_p3 ndhE 3 ACGTGGGAGCCATAAATGTC CAGTCCTCTGTTCTTGGAGC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.130239 124425 124727 1283-1283 0.500000 59.0 ndhE_p4 ndhE 4 ACGTGGGAGCCATAAATGTC GTCCTCTGTTCTTGGAGCAG 20 20 50.0 55.0 58.0 57.9 0.0 5.3 0.130239 124425 124727 1281-1281 0.500000 59.0 ndhE_p5 ndhE 5 CGAGATTGATGGGGAGTTGG GTCCTCTGTTCTTGGAGCAG 20 20 55.0 55.0 58.0 57.9 0.0 5.3 0.132811 124425 124727 713-713 0.500000 58.9 psaC_p1 psaC 1 GCTATTGGGCCAGCTATTGT GTAGTGATGAAGCCCGTCAG 20 20 50.0 55.0 58.0 58.1 6.2 0.0 0.085223 124971 125216 993-993 0.500000 59.3 psaC_p2 psaC 2 GCTATTGGGCCAGCTATTGT CCCCCTGCAGTGTACAAAAT 20 20 50.0 50.0 58.0 58.1 6.2 13.8 0.089762 124971 125216 1221-1221 0.500000 59.3 psaC_p3 psaC 3 GCTATTGGGCCAGCTATTGT AACCGCGAATCTCGAGTAAC 20 20 50.0 50.0 58.0 58.1 6.2 8.2 0.107668 124971 125216 1038-1038 0.500000 59.1 psaC_p4 psaC 4 GCTATTGGGCCAGCTATTGT CAACCGCGAATCTCGAGTAA 20 20 50.0 50.0 58.0 58.1 6.2 8.2 0.108974 124971 125216 1039-1039 0.500000 59.1 psaC_p5 psaC 5 CGAGATTGATGGGGAGTTGG GTAGTGATGAAGCCCGTCAG 20 20 55.0 55.0 58.0 58.1 0.0 0.0 0.113063 124971 125216 1238-1238 0.500000 59.1 psaC-ndhD_p1 psaC-ndhD 1 GCTATTGGGCCAGCTATTGT TTCCAATGCCACAGGATACG 20 20 50.0 50.0 58.0 58.0 6.2 0.0 0.050075 125217 125343 1330-1330 0.500000 59.6 psaC-ndhD_p2 psaC-ndhD 2 TCGAATGAGACAACTCGCAG TTCCAATGCCACAGGATACG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.054345 125217 125343 796-796 0.500000 59.6 psaC-ndhD_p3 psaC-ndhD 3 GCTATTGGGCCAGCTATTGT GTAGTGATGAAGCCCGTCAG 20 20 50.0 55.0 58.0 58.1 6.2 0.0 0.085223 125217 125343 993-993 0.500000 59.3 psaC-ndhD_p4 psaC-ndhD 4 TCGAATGAGACAACTCGCAG GTAGTGATGAAGCCCGTCAG 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.089493 125217 125343 459-459 0.500000 59.3 psaC-ndhD_p5 psaC-ndhD 5 GCTATTGGGCCAGCTATTGT CCCCCTGCAGTGTACAAAAT 20 20 50.0 50.0 58.0 58.1 6.2 13.8 0.089762 125217 125343 1221-1221 0.500000 59.3 ndhD_p1 ndhD 1 GCTATTGGGCCAGCTATTGT AGGTGCAAATTCGGCAATTG 20 20 50.0 45.0 58.0 57.9 6.2 0.0 0.096284 125344 126846 2585-2585 0.500000 59.2 ndhD_p2 ndhD 2 GCTATTGGGCCAGCTATTGT CAAGGTGCAAATTCGGCAAT 20 20 50.0 45.0 58.0 57.9 6.2 6.3 0.096284 125344 126846 2587-2587 0.500000 59.2 ndhD_p3 ndhD 3 GCTATTGGGCCAGCTATTGT GAGCGGTATGGGCTAATGAG 20 20 50.0 55.0 58.0 57.9 6.2 0.0 0.099172 125344 126846 2709-2709 0.500000 59.2 ndhD_p4 ndhD 4 TCGAATGAGACAACTCGCAG AGGTGCAAATTCGGCAATTG 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.100554 125344 126846 2051-2051 0.500000 59.2 ndhD_p5 ndhD 5 TCGAATGAGACAACTCGCAG CAAGGTGCAAATTCGGCAAT 20 20 50.0 45.0 58.0 57.9 0.0 6.3 0.100554 125344 126846 2053-2053 0.500000 59.2 ccsA_p1 ccsA 1 GGATTCTGGACCACGAGAAC GTGAAATCGGTAGACACGCT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.019370 127105 128067 1529-1529 0.500000 59.8 ccsA_p2 ccsA 2 GGATTCTGGACCACGAGAAC TGAAATCGGTAGACACGCTG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.020741 127105 128067 1528-1528 0.500000 59.8 ccsA_p3 ccsA 3 TTCTCGATGGCTTCTCTTGC GTGAAATCGGTAGACACGCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.023566 127105 128067 1753-1753 0.500000 59.8 ccsA_p4 ccsA 4 TTCTCGATGGCTTCTCTTGC TGAAATCGGTAGACACGCTG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.024937 127105 128067 1752-1752 0.500000 59.8 ccsA_p5 ccsA 5 TCAGTTGACAAGGTGGAAGC GTGAAATCGGTAGACACGCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.040512 127105 128067 1438-1438 0.500000 59.7 trnL-UAG-rpl32_p1 trnL-UAG-rpl32 1 AGCGTGTCTACCGATTTCAC GCACTCTATCCTCGAGCTTG 20 20 50.0 55.0 58.0 57.9 0.0 7.0 0.140570 128246 129181 1632-1632 0.500000 58.9 trnL-UAG-rpl32_p2 trnL-UAG-rpl32 2 CAGCGTGTCTACCGATTTCA GCACTCTATCCTCGAGCTTG 20 20 50.0 55.0 58.0 57.9 0.0 7.0 0.141941 128246 129181 1633-1633 0.500000 58.9 trnL-UAG-rpl32_p3 trnL-UAG-rpl32 3 AAAATAGTACTTGGCGCGGT GCACTCTATCCTCGAGCTTG 20 20 45.0 55.0 57.9 57.9 8.8 7.0 0.236277 128246 129181 2101-2101 0.500000 58.1 trnL-UAG-rpl32_p4 trnL-UAG-rpl32 4 AAATAGTACTTGGCGCGGTT GCACTCTATCCTCGAGCTTG 20 20 45.0 55.0 57.9 57.9 8.8 7.0 0.236277 128246 129181 2100-2100 0.500000 58.1 trnL-UAG-rpl32_p5 trnL-UAG-rpl32 5 TCCTAAGAGCAGCGTGTCTA GCACTCTATCCTCGAGCTTG 20 20 50.0 55.0 57.9 57.9 0.0 7.0 0.257017 128246 129181 1642-1642 0.500000 57.9 rpl32-ndhF_p1 rpl32-ndhF 1 CATCGGGTCATGCTCATTCA GGAACAAGCAGAAGTCCCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.061075 129335 130447 1405-1405 0.500000 59.5 rpl32-ndhF_p2 rpl32-ndhF 2 CATCGGGTCATGCTCATTCA CGAAAGGTATGACCCATGCA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.079289 129335 130447 1356-1356 0.500000 59.4 rpl32-ndhF_p3 rpl32-ndhF 3 CATCGGGTCATGCTCATTCA GCCCACATACGACGAAGATT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.106399 129335 130447 1438-1438 0.500000 59.1 rpl32-ndhF_p4 rpl32-ndhF 4 CATCGGGTCATGCTCATTCA ACGAACACATTCCCACTAGC 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.137094 129335 130447 1791-1791 0.500000 58.9 rpl32-ndhF_p5 rpl32-ndhF 5 CATCGGGTCATGCTCATTCA AGGAAAAGCCCACATACGAC 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.151756 129335 130447 1445-1445 0.500000 58.8 ndhF_p1 ndhF 1 CAAGCTCGAGGATAGAGTGC TTGCACACATGTCCAAGTGA 20 20 55.0 45.0 57.9 58.0 15.6 16.0 0.182628 130448 132670 2983-2983 0.500000 58.5 ndhF_p2 ndhF 2 TCAAGCCCTGTTTTTGGTGT TTGCACACATGTCCAAGTGA 20 20 45.0 45.0 58.1 58.0 0.0 16.0 0.190586 130448 132670 2694-2694 0.500000 58.5 ndhF_p3 ndhF 3 CAAGCTCGAGGATAGAGTGC GTGCACGACAAAACCTGAAC 20 20 55.0 50.0 57.9 58.2 15.6 0.0 0.310159 130448 132670 2885-2885 0.500000 57.5 ndhF_p4 ndhF 4 TCAAGCCCTGTTTTTGGTGT GTGCACGACAAAACCTGAAC 20 20 45.0 50.0 58.1 58.2 0.0 0.0 0.318117 130448 132670 2596-2596 0.500000 57.5 ndhF_p5 ndhF 5 CAAGCTCGAGGATAGAGTGC ACCTGAACCTGAAGACCTGT 20 20 55.0 50.0 57.9 58.2 15.6 0.0 0.324926 130448 132670 2873-2873 0.500000 57.4