primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AATGTTCCCGCCATTCGTTA	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.285247	3937	4563	762-772	1.000000	80.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TGTTCCCGCCATTCGTTATT	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.285247	3937	4563	760-770	1.000000	80.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AATGTTCCCGCCATTCGTTA	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.285247	3937	4563	761-771	1.000000	80.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TAAATGTTCCCGCCATTCGT	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.285247	3937	4563	763-773	1.000000	80.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TGTTCCCGCCATTCGTTATT	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.285247	3937	4563	759-769	1.000000	80.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5678	6518	916-925	1.000000	79.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5678	6518	917-926	1.000000	75.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	58.2	57.3	0.0	16.9	0.871706	5678	6518	920-929	1.000000	75.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.883726	5678	6518	946-955	1.000000	74.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	AAAACCTAAATGGGGCGTGG	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.941815	5678	6518	974-983	1.000000	74.4
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	GGGGAATCCATAGAGGGTCA	20	20	45.0	55.0	58.0	57.9	1.4	0.1	0.156790	13824	14297	533-534	1.000000	80.8
atpH-atpI_p2	atpH-atpI	2	CGCAATACCTTCTACGGCTT	GGGGAATCCATAGAGGGTCA	20	20	50.0	55.0	58.1	57.9	0.0	0.1	0.182471	13824	14297	621-622	1.000000	80.3
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	GGGGAATCCATAGAGGGTCA	20	20	50.0	55.0	57.9	57.9	0.0	0.1	0.230682	13824	14297	589-590	1.000000	80.1
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	GGGGAATCCATAGAGGGTCA	20	20	45.0	55.0	58.2	57.9	1.4	0.1	0.302329	13824	14297	534-535	1.000000	79.7
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	GGGGAATCCATAGAGGGTCA	20	20	55.0	55.0	58.2	57.9	0.0	0.1	0.308719	13824	14297	593-594	1.000000	79.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	TGCTATACACCTTGCTGGGA	20	20	45.0	50.0	55.8	58.4	0.0	2.7	2.650401	29852	30550	836-837	1.000000	59.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	29852	30550	810-811	1.000000	58.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	ACACCTTGCTGGGATTGTAG	20	20	45.0	50.0	55.8	57.5	0.0	2.7	2.726197	29852	30550	830-831	1.000000	58.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	20	20	45.0	50.0	55.8	57.5	0.0	2.7	2.726197	29852	30550	829-830	1.000000	58.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	ATGCTATACACCTTGCTGGG	20	20	45.0	50.0	55.8	57.4	0.0	1.7	2.875136	29852	30550	837-838	1.000000	57.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31156	31636	583-585	1.000000	79.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	31156	31636	562-564	1.000000	79.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	31156	31636	559-561	1.000000	79.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31156	31636	580-582	1.000000	78.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.452638	31156	31636	585-587	1.000000	77.5
rps14-psaB_p1	rps14-psaB	1	ATTTCTGCCGCTTCTTCTCC	TTGATTGCCTCCACATCAGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.389286	36707	36843	216-216	1.000000	78.0
rps14-psaB_p2	rps14-psaB	2	CGACGGAACCCTGCTTATTT	TTGATTGCCTCCACATCAGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.399119	36707	36843	278-278	1.000000	77.7
rps14-psaB_p3	rps14-psaB	3	ATTTCTGCCGCTTCTTCTCC	GCAGCTTTCTTGATTGCCTC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.530924	36707	36843	225-225	1.000000	76.8
rps14-psaB_p4	rps14-psaB	4	CGACGGAACCCTGCTTATTT	GCAGCTTTCTTGATTGCCTC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.540757	36707	36843	287-287	1.000000	76.5
rps14-psaB_p5	rps14-psaB	5	TTTCACTCAACGACGGAACC	TTGATTGCCTCCACATCAGG	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.559147	36707	36843	288-288	1.000000	76.4
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.110498	41327	41968	820-821	1.000000	79.5
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	20	20	50.0	50.0	57.9	57.9	0.0	12.9	0.214563	41327	41968	818-819	1.000000	78.7
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.258580	41327	41968	747-748	1.000000	78.4
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.286351	41327	41968	751-752	1.000000	78.1
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.384749	41327	41968	746-747	1.000000	77.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAGAAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.1	0.0	0.063299	45858	46713	957-1000	1.000000	80.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATAGAGAAAAAGCCGGCT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	27.1	0.0	0.063299	45858	46713	961-1004	1.000000	80.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TCGATAGAGAAAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	21.9	0.0	0.068691	45858	46713	962-1005	1.000000	80.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AGAGAAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.1	0.0	0.128108	45858	46713	965-1008	1.000000	80.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGATAGAGAAAAAGCCGGCT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	27.1	0.0	0.128108	45858	46713	969-1012	1.000000	80.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCAAGATAGGAATAGCACCCG	AGAAGGTCTACGGTTCGAGT	21	20	52.4	50.0	58.0	57.8	0.0	0.0	1.242884	50159	51849	1780-1794	1.000000	71.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTCCTTCATTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	57.8	57.8	15.3	0.0	1.391288	50159	51849	1824-1838	1.000000	70.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GCCAAGATAGGAATAGCACCC	AGAAGGTCTACGGTTCGAGT	21	20	52.4	50.0	58.2	57.8	0.0	0.0	1.412936	50159	51849	1781-1795	1.000000	69.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCAAGATAGGAATAGCACCCG	AGCCCAATGCAACAAAATGC	21	20	52.4	45.0	58.0	58.5	0.0	0.0	1.519660	50159	51849	1862-1876	1.000000	69.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCAAGATAGGAATAGCACCCG	GAAGGTCTACGGTTCGAGTC	21	20	52.4	55.0	58.0	57.5	0.0	0.0	1.586656	50159	51849	1779-1793	1.000000	68.5
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	6.4	0.0	0.541273	64448	65790	1464-1490	1.000000	77.2
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.578888	64448	65790	1477-1503	1.000000	76.9
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	20	20	45.0	45.0	58.2	57.5	0.0	0.0	0.660959	64448	65790	1473-1499	1.000000	76.3
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.677289	64448	65790	1472-1498	1.000000	76.1
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.727231	64448	65790	1466-1492	1.000000	75.8
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AGGCGCTGTGGAAAGATATG	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.140713	66540	66899	450-451	1.000000	80.1
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	ACCCGTGACATTTTGTACCC	CAGGCGCTGTGGAAAGATAT	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.140713	66540	66899	451-452	1.000000	80.1
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CCCGTGACATTTTGTACCCA	AGGCGCTGTGGAAAGATATG	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.142057	66540	66899	449-450	1.000000	80.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	CAGGCGCTGTGGAAAGATAT	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.142057	66540	66899	450-451	1.000000	80.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACAGGATTTGAACCCGTGAC	AGGCGCTGTGGAAAGATATG	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.148749	66540	66899	461-462	1.000000	80.0
infA-rps8_p1	infA-rps8	1	GGTATCAAACTGCGTCGGAT	CTTTCCACTTCTCGAGGCAT	20	20	50.0	50.0	58.1	57.6	0.0	13.6	0.457339	79755	79768	218-218	1.000000	76.7
infA-rps8_p2	infA-rps8	2	TCTAAAAGGACTCGGACCGT	CTTTCCACTTCTCGAGGCAT	20	20	50.0	50.0	58.1	57.6	14.8	13.6	0.478131	79755	79768	161-161	1.000000	76.7
infA-rps8_p3	infA-rps8	3	GGTATCAAACTGCGTCGGAT	TTCTTTCCACTTCTCGAGGC	20	20	50.0	50.0	58.1	57.5	0.0	13.6	0.521433	79755	79768	220-220	1.000000	76.2
infA-rps8_p4	infA-rps8	4	TCTAAAAGGACTCGGACCGT	TTCTTTCCACTTCTCGAGGC	20	20	50.0	50.0	58.1	57.5	14.8	13.6	0.542225	79755	79768	163-163	1.000000	76.2
infA-rps8_p5	infA-rps8	5	GACCGTTGGGAAGTGATTCA	CTTTCCACTTCTCGAGGCAT	20	20	50.0	50.0	57.8	57.6	0.0	13.6	0.572183	79755	79768	147-147	1.000000	76.0
rpl14_p1	rpl14	1	TCAGCAATAACGTCTCTGCC	ATACGCTCTTCAGGCACTTG	20	20	50.0	50.0	58.0	58.0	10.9	0.0	0.025881	80344	80712	1019-1019	1.000000	80.5
rpl14_p2	rpl14	2	TCAGCAATAACGTCTCTGCC	GACAAATAGAAGCAGGGCGA	20	20	50.0	50.0	58.0	58.0	10.9	0.0	0.034321	80344	80712	978-978	1.000000	80.5
rpl14_p3	rpl14	3	CGGGTCGACTGATACGTTTT	ATACGCTCTTCAGGCACTTG	20	20	50.0	50.0	58.0	58.0	16.5	0.0	0.029038	80344	80712	1246-1246	1.000000	80.3
rpl14_p4	rpl14	4	CGGGTCGACTGATACGTTTT	GACAAATAGAAGCAGGGCGA	20	20	50.0	50.0	58.0	58.0	16.5	0.0	0.037478	80344	80712	1205-1205	1.000000	80.3
rpl14_p5	rpl14	5	TCAGCAATAACGTCTCTGCC	ATTGAGATAGCTGCGTGCAA	20	20	50.0	45.0	58.0	58.0	10.9	0.0	0.038565	80344	80712	760-760	0.250000	49.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGTATTTAGCTGCAATAAAAGC	AGCGTGTCTACCGATTTCAC	24	20	37.5	50.0	57.9	58.0	1.0	0.0	4.156959	113373	113990	750-1056	1.000000	48.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTATTTAGCTGCAATAAAAGC	CAGCGTGTCTACCGATTTCA	24	20	37.5	50.0	57.9	58.0	1.0	0.0	4.158330	113373	113990	751-1057	1.000000	48.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGTATTTAGCTGCAATAAAAGC	TTTACAGTATGCCGCCACTC	24	20	37.5	50.0	57.9	58.0	1.0	0.0	4.167671	113373	113990	810-1116	1.000000	48.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTGCGACAAACAAATAAGA	AGCGTGTCTACCGATTTCAC	20	20	35.0	50.0	53.8	58.0	0.0	0.0	4.234839	113373	113990	678-984	1.000000	48.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTGCGACAAACAAATAAGA	CAGCGTGTCTACCGATTTCA	20	20	35.0	50.0	53.8	58.0	0.0	0.0	4.236209	113373	113990	679-985	1.000000	48.2
ccsA_p1	ccsA	1	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCCT	20	21	50.0	42.9	58.0	56.3	17.2	6.6	2.739927	114149	115120	1094-1094	1.000000	58.3
ccsA_p2	ccsA	2	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCCTT	20	22	50.0	40.9	58.0	57.0	17.2	6.6	3.015330	114149	115120	1094-1094	1.000000	56.1
ccsA_p3	ccsA	3	AGTGGCGGCATACTGTAAAA	ACGACGGTCTTATGTATTCCT	20	21	45.0	42.9	57.5	56.3	17.2	6.6	3.204400	114149	115120	1093-1093	1.000000	54.6
ccsA_p4	ccsA	4	GAGTGGCGGCATACTGTAAA	ACGACGGTCTTATGTATTCC	20	20	50.0	45.0	58.0	54.7	17.2	6.6	3.307376	114149	115120	1094-1094	1.000000	53.8
ccsA_p5	ccsA	5	GAGTGGCGGCATACTGTAAA	GTACGACGGTCTTATGTATTCCT	20	23	50.0	43.5	58.0	57.6	17.2	6.6	3.448499	114149	115120	1096-1096	1.000000	52.6
ccsA-ndhD_p1	ccsA-ndhD	1	TGGGACCCCAAAGAAACTTG	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	57.9	58.0	8.4	0.0	0.096390	115121	115343	793-793	1.000000	80.6
ccsA-ndhD_p2	ccsA-ndhD	2	AGTGCCATTTTTACCCAGGG	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.1	58.0	2.9	0.0	0.078545	115121	115343	1246-1246	1.000000	80.3
ccsA-ndhD_p3	ccsA-ndhD	3	GCCCCAAGTGCCATTTTTAC	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.109042	115121	115343	1252-1252	1.000000	80.0
ccsA-ndhD_p4	ccsA-ndhD	4	AGTGCCATTTTTACCCAGGG	CTCGATGGCTTCTCTTGCAT	20	20	50.0	50.0	58.1	58.0	2.9	0.0	0.111981	115121	115343	1244-1244	1.000000	80.0
ccsA-ndhD_p5	ccsA-ndhD	5	GTTTCACCACATCGGGTCTT	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.123526	115121	115343	1227-1227	1.000000	79.9
ycf1_p1	ycf1	1	TGATTGGTATGATCCACGGG	ATACGCATATCGGCACCAAG	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.133033	124781	124900	267-267	1.000000	71.8
ycf1_p2	ycf1	2	GATTGGTATGATCCACGGGT	ATACGCATATCGGCACCAAG	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.135059	124781	124900	266-266	1.000000	71.8
ycf1_p3	ycf1	3	TGATTGGTATGATCCACGGG	AATACGCATATCGGCACCAA	20	20	50.0	45.0	57.1	57.8	0.0	0.0	1.177081	124781	124900	268-268	1.000000	71.5
ycf1_p4	ycf1	4	GATTGGTATGATCCACGGGT	AATACGCATATCGGCACCAA	20	20	50.0	45.0	57.1	57.8	0.0	0.0	1.179107	124781	124900	267-267	1.000000	71.5
ycf1_p5	ycf1	5	TTGATTGGTATGATCCACGGG	ATACGCATATCGGCACCAAG	21	20	47.6	50.0	57.8	58.2	0.0	0.0	1.411497	124781	124900	268-268	1.000000	69.6