primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 99 493 464-635 1.000000 85.5 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 ACTGCCTTAATCCACTTGGC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 58.2 57.9 0.0 0.0 0.235305 99 493 465-636 1.000000 85.2 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.7 57.9 0.0 0.0 0.406792 99 493 457-628 1.000000 83.9 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 99 493 513-684 1.000000 83.1 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 58.2 58.3 0.0 0.0 0.496882 99 493 514-685 1.000000 82.9 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 TCCCTCGCTTCATCCAAATG TTGAAAAAGGTGCTCAGCCT 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.114464 4359 5274 1054-1098 1.000000 85.3 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 TCCCTCGCTTCATCCAAATG AAAAAGGTGCTCAGCCTACC 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.131629 4359 5274 1051-1095 1.000000 85.1 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CCGATGGTATGGACGAATCC TTGAAAAAGGTGCTCAGCCT 20 20 55.0 45.0 57.9 57.9 0.0 0.0 0.152064 4359 5274 1071-1115 1.000000 85.0 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CGATGGTATGGACGAATCCC TTGAAAAAGGTGCTCAGCCT 20 20 55.0 45.0 57.9 57.9 0.0 0.0 0.152064 4359 5274 1070-1114 1.000000 85.0 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CGATGGTATGGACGAATCCC AAAAAGGTGCTCAGCCTACC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.169229 4359 5274 1067-1111 1.000000 84.8 atpF-atpH_p1 atpF-atpH 1 TGGCCCAAAGAAACGAAAGA GCCTGGTTGTAGCATTAGCA 20 20 45.0 50.0 57.9 58.2 0.0 0.0 0.309857 13858 14231 452-491 1.000000 81.7 atpF-atpH_p2 atpF-atpH 2 GTGGCCCAAAGAAACGAAAG GCCTGGTTGTAGCATTAGCA 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.384008 13858 14231 453-492 1.000000 81.1 atpF-atpH_p3 atpF-atpH 3 ATTAAACCCGAAACTCCCGG GCCTGGTTGTAGCATTAGCA 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.368120 13858 14231 483-522 1.000000 81.0 atpF-atpH_p4 atpF-atpH 4 TGGCCCAAAGAAACGAAAGA TGGCCTGGTTGTAGCATTAG 20 20 45.0 50.0 57.9 57.6 0.0 3.3 0.478418 13858 14231 454-493 1.000000 80.3 atpF-atpH_p5 atpF-atpH 5 TGGCCCAAAGAAACGAAAGA CAGAGGGCAAAATACGAGGT 20 20 45.0 50.0 57.9 57.6 0.0 0.0 0.463302 13858 14231 518-557 1.000000 80.1 rps2-rpoC2_p1 rps2-rpoC2 1 ATCGTTCGCTGGAATCGAAA GGTTGAGGGTTGGAACGAAT 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.098945 17301 17533 1518-1523 1.000000 80.7 rps2-rpoC2_p2 rps2-rpoC2 2 TCGTTCGCTGGAATCGAAAT GGTTGAGGGTTGGAACGAAT 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.098945 17301 17533 1517-1522 1.000000 80.7 rps2-rpoC2_p3 rps2-rpoC2 3 ATCGTTCGCTGGAATCGAAA CTCGAAAAGAGGGTTGAGGG 20 20 45.0 55.0 58.0 57.9 0.0 0.0 0.105187 17301 17533 1529-1534 1.000000 80.6 rps2-rpoC2_p4 rps2-rpoC2 4 TCGTTCGCTGGAATCGAAAT CTCGAAAAGAGGGTTGAGGG 20 20 45.0 55.0 58.0 57.9 0.0 0.0 0.105187 17301 17533 1528-1533 1.000000 80.6 rps2-rpoC2_p5 rps2-rpoC2 5 CGAGATCGGGGTCACAATTT GGTTGAGGGTTGGAACGAAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.118538 17301 17533 1492-1497 1.000000 80.6 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 AACTTCCTTTCGCAGTACCC GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.186403 32425 33262 953-994 1.000000 88.5 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.287509 32425 33262 887-928 1.000000 88.1 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 AACTTCCTTTCGCAGTACCC CCGATGACTTACGCCTTACC 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.331336 32425 33262 950-991 1.000000 87.4 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 20 20 50.0 55.0 57.7 58.2 0.0 0.0 0.432442 32425 33262 884-925 1.000000 87.0 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 AACTTCCTTTCGCAGTACCC TTGAACCGATGACTTACGCC 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.463238 32425 33262 955-996 1.000000 86.3 trnM-CAU-psbD_p1 trnM-CAU-psbD 1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 20 20 50.0 40.0 58.3 53.0 0.0 6.7 5.273069 33328 34756 1209-1483 1.000000 47.8 trnM-CAU-psbD_p2 trnM-CAU-psbD 2 GGCGTAAGTCATCGGTTCAA TTACCAAGGGCTATAGTCAT 20 20 50.0 40.0 58.3 52.8 0.0 6.7 5.524429 33328 34756 1208-1482 1.000000 47.8 trnM-CAU-psbD_p3 trnM-CAU-psbD 3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCAT 20 21 50.0 38.1 58.3 53.7 0.0 6.7 5.575652 33328 34756 1209-1483 1.000000 47.8 trnM-CAU-psbD_p4 trnM-CAU-psbD 4 CCCCTTTAACTCAGCGGTAG TTTACCAAGGGCTATAGTCA 20 20 55.0 40.0 57.7 53.0 0.0 6.7 5.301387 33328 34756 1245-1519 1.000000 47.7 trnM-CAU-psbD_p5 trnM-CAU-psbD 5 CCCCTTTAACTCAGCGGTAG TTACCAAGGGCTATAGTCAT 20 20 55.0 40.0 57.7 52.8 0.0 6.7 5.552747 33328 34756 1244-1518 1.000000 47.7 psbC-trnS-UGA_p1 psbC-trnS-UGA 1 GCAGCAGGGTTTGAAAAAGG GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.151581 37188 37418 389-404 1.000000 82.9 psbC-trnS-UGA_p2 psbC-trnS-UGA 2 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 20 20 55.0 55.0 58.2 58.0 0.0 0.0 0.181932 37188 37418 350-365 1.000000 82.8 psbC-trnS-UGA_p3 psbC-trnS-UGA 3 GCAGCAGGGTTTGAAAAAGG GCTGAGTGGTTGATAGCTCC 20 20 50.0 55.0 58.1 58.1 0.0 0.0 0.183481 37188 37418 381-396 1.000000 82.7 psbC-trnS-UGA_p4 psbC-trnS-UGA 4 GCAGCAGGGTTTGAAAAAGG GGCTGAGTGGTTGATAGCTC 20 20 50.0 55.0 58.1 58.1 0.0 0.0 0.183481 37188 37418 382-397 1.000000 82.7 psbC-trnS-UGA_p5 psbC-trnS-UGA 5 GCAGCAGGGTTTGAAAAAGG GGTTGATAGCTCCGGTCTTG 20 20 50.0 55.0 58.1 58.1 0.0 0.0 0.188901 37188 37418 374-389 1.000000 82.7 psaA-ycf3_p1 psaA-ycf3 1 TGAGAAATGACCCGGTTTGG GGAAACAGGCTATAGCGCTT 20 20 50.0 50.0 58.1 58.0 0.0 18.7 0.140786 43734 44473 904-934 1.000000 82.9 psaA-ycf3_p2 psaA-ycf3 2 TGAGAAATGACCCGGTTTGG ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.136672 43734 44473 922-952 1.000000 82.8 psaA-ycf3_p3 psaA-ycf3 3 TGAGAAATGACCCGGTTTGG CGATCAAGCCGCTGAGTATT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.237622 43734 44473 924-954 1.000000 82.0 psaA-ycf3_p4 psaA-ycf3 4 TGAGAAATGACCCGGTTTGG AGCCGCTGAGTATTGGAAAC 20 20 50.0 50.0 58.1 58.3 0.0 0.0 0.356647 43734 44473 918-948 1.000000 81.1 psaA-ycf3_p5 psaA-ycf3 5 TGAGAAATGACCCGGTTTGG CAAGCCGCTGAGTATTGGAA 20 20 50.0 50.0 58.1 58.3 0.0 0.0 0.357875 43734 44473 920-950 1.000000 81.1 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 21 20 47.6 50.0 58.0 58.1 8.2 0.0 1.134847 46447 47311 748-955 1.000000 82.8 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 21 20 47.6 50.0 58.0 58.2 8.2 0.0 1.191833 46447 47311 769-976 1.000000 82.1 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 21 20 47.6 45.0 58.0 57.8 8.2 5.6 1.233148 46447 47311 719-926 1.000000 82.1 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 21 20 47.6 50.0 58.0 58.4 8.2 0.0 1.427808 46447 47311 749-956 1.000000 80.4 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 TTTATCCGCGATCTAGGCAT CCCTCGGTAAACAAAAGCCT 20 20 45.0 50.0 56.6 58.1 8.2 0.0 1.514239 46447 47311 746-953 1.000000 79.7 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 48719 49506 811-856 1.000000 85.2 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 48719 49506 819-864 1.000000 84.7 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 48719 49506 843-888 1.000000 84.3 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 48719 49506 851-896 1.000000 83.7 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 48719 49506 825-870 1.000000 83.3 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA TGGATAGGATGGCCTTTACG 20 20 55.0 50.0 58.0 56.8 10.3 3.3 1.224095 50548 51242 820-839 1.000000 75.3 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 GGGATAGCTCAGCTGGTAGA GGATAGGATGGCCTTTACGT 20 20 55.0 50.0 58.0 56.8 10.3 3.3 1.226313 50548 51242 819-838 1.000000 75.3 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 20 20 50.0 50.0 58.1 56.8 0.0 3.3 1.273485 50548 51242 784-803 1.000000 75.2 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 ATGTGGCATCGGTAATGGTC TGGATAGGATGGCCTTTACG 20 20 50.0 50.0 58.0 56.8 0.0 3.3 1.254271 50548 51242 840-859 1.000000 75.0 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 ATGTGGCATCGGTAATGGTC GGATAGGATGGCCTTTACGT 20 20 50.0 50.0 58.0 56.8 0.0 3.3 1.256489 50548 51242 839-858 1.000000 75.0 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 CGCTAATCGGGGCTAAAACT CCGAGAATGTCTACGGTTCG 20 20 50.0 55.0 58.1 58.2 0.0 0.0 0.226706 52923 54113 1209-1353 1.000000 83.7 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCGCTAATCGGGGCTAAAAC CCGAGAATGTCTACGGTTCG 20 20 50.0 55.0 58.1 58.2 0.0 0.0 0.232140 52923 54113 1210-1354 1.000000 83.7 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 CTCTGGACCTTCGCTAATCG CCGAGAATGTCTACGGTTCG 20 20 55.0 55.0 57.9 58.2 0.0 0.0 0.303085 52923 54113 1220-1364 1.000000 83.1 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 GGGCTAAAACTCCGGAAAGT CCGAGAATGTCTACGGTTCG 20 20 50.0 55.0 57.8 58.2 5.2 0.0 0.371913 52923 54113 1200-1344 1.000000 82.6 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CTTCGCTAATCGGGGCTAAA CCGAGAATGTCTACGGTTCG 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.410471 52923 54113 1212-1356 1.000000 82.2 petA-psbJ_p1 petA-psbJ 1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.0 57.6 0.0 4.1 0.403359 66120 67152 1095-1178 1.000000 81.8 petA-psbJ_p2 petA-psbJ 2 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 57.8 57.6 0.0 4.1 0.519826 66120 67152 1078-1161 1.000000 80.9 petA-psbJ_p3 petA-psbJ 3 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.2 57.6 13.8 4.1 0.543756 66120 67152 1092-1175 1.000000 80.7 petA-psbJ_p4 petA-psbJ 4 TTACGTGTCCAAGGCCTTTT ATTCCGGATTGGGTTCATCC 20 20 45.0 50.0 57.6 57.6 17.6 4.1 0.716678 66120 67152 1090-1173 1.000000 79.3 petA-psbJ_p5 petA-psbJ 5 TACGTGTCCAAGGCCTTTTG ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.4 57.6 17.6 4.1 0.764949 66120 67152 1089-1172 1.000000 78.9 trnN-GUU-nad5_p1 trnN-GUU-nad5 1 AGGATTCGAACCTACGACCA CGGGCGCATTTCTTCATATC 20 20 50.0 50.0 58.2 57.4 15.0 10.8 0.758112 112159 113716 1638-1718 1.000000 83.8 trnN-GUU-nad5_p2 trnN-GUU-nad5 2 CCCCAAGTAGGATTCGAACC CGGGCGCATTTCTTCATATC 20 20 55.0 50.0 57.7 57.4 0.0 10.8 0.928680 112159 113716 1646-1726 1.000000 82.4 trnN-GUU-nad5_p3 trnN-GUU-nad5 3 GGATTCGAACCTACGACCAA CGGGCGCATTTCTTCATATC 20 20 50.0 50.0 57.3 57.4 0.0 10.8 1.261669 112159 113716 1637-1717 1.000000 79.7 trnN-GUU-nad5_p4 trnN-GUU-nad5 4 AGGATTCGAACCTACGACCA GGTATCAAATCTGTAGGGGGC 20 21 50.0 52.4 58.2 57.9 15.0 0.0 1.298399 112159 113716 1658-1738 1.000000 79.4 trnN-GUU-nad5_p5 trnN-GUU-nad5 5 AGGATTCGAACCTACGACCA TATCAAATCTGTAGGGGGCG 20 20 50.0 50.0 58.2 56.8 15.0 0.0 1.378221 112159 113716 1656-1736 1.000000 78.8 nad5-rpl32_p1 nad5-rpl32 1 CTGGAAGTGGAAGGAAAGGT ACAAAGCTTTTAACGCTGCC 20 20 50.0 45.0 57.0 57.9 0.0 9.5 1.093666 115958 117120 1312-1418 1.000000 77.8 nad5-rpl32_p2 nad5-rpl32 2 CTGGAAGTGGAAGGAAAGGT GCCCAATACCCCTTCCTTTT 20 20 50.0 50.0 57.0 57.8 0.0 0.0 1.207164 115958 117120 1295-1401 1.000000 77.0 nad5-rpl32_p3 nad5-rpl32 3 CTGGAAGTGGAAGGAAAGGT TTTAACGCTGCCCAATACCC 20 20 50.0 50.0 57.0 58.5 0.0 0.0 1.493671 115958 117120 1304-1410 1.000000 74.6 nad5-rpl32_p4 nad5-rpl32 4 CTGGAAGTGGAAGGAAAGGT CAAAGCTTTTAACGCTGCCC 20 20 50.0 50.0 57.0 58.6 0.0 9.5 1.541093 115958 117120 1311-1417 1.000000 74.3 nad5-rpl32_p5 nad5-rpl32 5 CTGGAAGTGGAAGGAAAGGT AGCTTTTAACGCTGCCCAAT 20 20 50.0 45.0 57.0 58.7 0.0 0.0 1.702653 115958 117120 1308-1414 1.000000 73.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 57.9 58.0 13.5 0.0 0.142032 117286 118218 905-1079 1.000000 85.3 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 57.9 58.0 13.5 0.0 0.143402 117286 118218 906-1080 1.000000 85.3 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAAAGGAAGGGGTATTGGGC AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.255529 117286 118218 922-1096 1.000000 84.3 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AAAAGGAAGGGGTATTGGGC CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.256900 117286 118218 923-1097 1.000000 84.3 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 57.9 57.9 13.5 0.0 0.258478 117286 118218 915-1089 1.000000 84.3 ycf1_p1 ycf1 1 TCATCATTACACAATCGAGTCCT ATGCCAGAGTGATGGAAAGC 23 20 39.1 50.0 57.4 58.2 0.0 0.0 3.838225 132145 132264 261-261 1.000000 53.3 ycf1_p2 ycf1 2 TCATCATTACACAATCGAGTCCT GCCAGAGTGATGGAAAGCAA 23 20 39.1 50.0 57.4 58.5 0.0 0.0 4.060369 132145 132264 259-259 1.000000 51.5 ycf1_p3 ycf1 3 TCATCATTACACAATCGAGTCC ATGCCAGAGTGATGGAAAGC 22 20 40.9 50.0 56.0 58.2 0.0 0.0 4.240596 132145 132264 261-261 1.000000 50.1 ycf1_p4 ycf1 4 CATCATTACACAATCGAGTCCT ATGCCAGAGTGATGGAAAGC 22 20 40.9 50.0 56.0 58.2 0.0 0.0 4.250410 132145 132264 260-260 1.000000 50.0 ycf1_p5 ycf1 5 TCATCATTACACAATCGAGTCCTT ATGCCAGAGTGATGGAAAGC 24 20 37.5 50.0 58.0 58.2 0.0 0.0 4.262173 132145 132264 261-261 1.000000 49.9