primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Gly_p1	tRNA-Ser-tRNA-Gly	1	ATTAGCAATCCGACGCGTTA	TACCACTAAACCATACCCGC	20	20	45.0	50.0	57.8	57.0	15.5	0.0	1.198826	9330	10153	869-884	1.000000	77.9
tRNA-Ser-tRNA-Gly_p2	tRNA-Ser-tRNA-Gly	2	TTAGACCTCTCGGCCATCTC	TACCACTAAACCATACCCGC	20	20	55.0	50.0	58.3	57.0	2.2	0.0	1.292331	9330	10153	852-867	1.000000	77.3
tRNA-Ser-tRNA-Gly_p3	tRNA-Ser-tRNA-Gly	3	TTAGCAATCCGACGCGTTAG	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.4	57.0	15.5	0.0	1.433732	9330	10153	868-883	1.000000	76.0
tRNA-Ser-tRNA-Gly_p4	tRNA-Ser-tRNA-Gly	4	ATTAGCAATCCGACGCGTTA	TTACCACTAAACCATACCCGC	20	21	45.0	47.6	57.8	57.7	15.5	0.0	1.477867	9330	10153	870-885	1.000000	75.7
tRNA-Ser-tRNA-Gly_p5	tRNA-Ser-tRNA-Gly	5	TTAGACCTCTCGGCCATCTC	TTACCACTAAACCATACCCGC	20	21	55.0	47.6	58.3	57.7	2.2	0.0	1.571372	9330	10153	853-868	1.000000	75.1
tRNA-Gly-tRNA-Gly_p1	tRNA-Gly-tRNA-Gly	1	TTACTTTGTGGCGGGTATGG	CCCGCATCATTAGCTTGGAA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.437688	10177	11015	900-924	1.000000	84.6
tRNA-Gly-tRNA-Gly_p2	tRNA-Gly-tRNA-Gly	2	TTGTGGCGGGTATGGTTTAG	CCCGCATCATTAGCTTGGAA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.437688	10177	11015	895-919	1.000000	84.6
tRNA-Gly-tRNA-Gly_p3	tRNA-Gly-tRNA-Gly	3	TTACTTTGTGGCGGGTATGG	ATCGAACCCGCATCATTAGC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.603901	10177	11015	906-930	1.000000	83.2
tRNA-Gly-tRNA-Gly_p4	tRNA-Gly-tRNA-Gly	4	TTGTGGCGGGTATGGTTTAG	ATCGAACCCGCATCATTAGC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.603901	10177	11015	901-925	1.000000	83.2
tRNA-Gly-tRNA-Gly_p5	tRNA-Gly-tRNA-Gly	5	TTGTGGCGGGTATGGTTTAG	AACCCGCATCATTAGCTTGG	20	20	50.0	50.0	57.9	58.6	0.0	0.0	0.724238	10177	11015	897-921	1.000000	82.3
tRNA-Gly-tRNA-Arg_p1	tRNA-Gly-tRNA-Arg	1	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.084759	11064	11207	230-233	1.000000	75.3
tRNA-Gly-tRNA-Arg_p2	tRNA-Gly-tRNA-Arg	2	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.168087	11064	11207	236-239	1.000000	74.7
tRNA-Gly-tRNA-Arg_p3	tRNA-Gly-tRNA-Arg	3	TTCCAAGCTAATGATGCGGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.3	56.9	0.0	0.0	2.372466	11064	11207	220-223	1.000000	73.0
tRNA-Gly-tRNA-Arg_p4	tRNA-Gly-tRNA-Arg	4	TTCCAAGCTAATGATGCGGG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.3	57.9	0.0	0.0	2.455793	11064	11207	226-229	1.000000	72.4
tRNA-Gly-tRNA-Arg_p5	tRNA-Gly-tRNA-Arg	5	GCTAATGATGCGGGTTCGAT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.5	56.9	0.0	0.0	2.538678	11064	11207	214-217	1.000000	71.7
tRNA-Asp-tRNA-Tyr_p1	tRNA-Asp-tRNA-Tyr	1	TCAATTGGTTAGAGCACCGC	CTCGAGTGGTTAATGGGGAC	20	20	50.0	55.0	58.6	57.7	5.4	3.6	0.862140	28787	28902	238-252	1.000000	83.6
tRNA-Asp-tRNA-Tyr_p2	tRNA-Asp-tRNA-Tyr	2	TCAATTGGTTAGAGCACCGC	ATGCTCGAGTGGTTAATGGG	20	20	50.0	50.0	58.6	57.7	5.4	15.1	0.883079	28787	28902	241-255	1.000000	83.4
tRNA-Asp-tRNA-Tyr_p3	tRNA-Asp-tRNA-Tyr	3	GGTTCGAGACCCGTCAATC	CTCGAGTGGTTAATGGGGAC	19	20	57.9	55.0	58.0	57.7	5.7	3.6	1.357404	28787	28902	197-211	1.000000	81.0
tRNA-Asp-tRNA-Tyr_p4	tRNA-Asp-tRNA-Tyr	4	GTTCGAGACCCGTCAATCC	CTCGAGTGGTTAATGGGGAC	19	20	57.9	55.0	58.0	57.7	0.0	3.6	1.357404	28787	28902	196-210	1.000000	81.0
tRNA-Asp-tRNA-Tyr_p5	tRNA-Asp-tRNA-Tyr	5	GTTCGAGACCCGTCAATCC	ATGCTCGAGTGGTTAATGGG	19	20	57.9	50.0	58.0	57.7	0.0	15.1	1.378343	28787	28902	199-213	1.000000	80.7
tRNA-Glu-tRNA-Cys_p1	tRNA-Glu-tRNA-Cys	1	TTGTCCTAGGCCTCTAGACG	CATCCCCCAGTTCGAATCTG	20	20	55.0	55.0	57.9	58.0	9.7	16.2	0.095190	29234	30795	1541-1620	1.000000	91.2
tRNA-Glu-tRNA-Cys_p2	tRNA-Glu-tRNA-Cys	2	TTGTCCTAGGCCTCTAGACG	TCATCCCCCAGTTCGAATCT	20	20	55.0	50.0	57.9	58.1	9.7	0.0	0.174657	29234	30795	1542-1621	1.000000	90.6
tRNA-Glu-tRNA-Cys_p3	tRNA-Glu-tRNA-Cys	3	TTGTCCTAGGCCTCTAGACG	TTGCAAATCCTTCATCCCCC	20	20	55.0	50.0	57.9	58.1	9.7	0.0	0.189563	29234	30795	1553-1632	1.000000	90.5
tRNA-Glu-tRNA-Cys_p4	tRNA-Glu-tRNA-Cys	4	AGAGAGTTGTCCTAGGCCTC	CATCCCCCAGTTCGAATCTG	20	20	55.0	55.0	57.8	58.0	9.7	16.2	0.193992	29234	30795	1547-1626	1.000000	90.4
tRNA-Glu-tRNA-Cys_p5	tRNA-Glu-tRNA-Cys	5	GAGAGTTGTCCTAGGCCTCT	CATCCCCCAGTTCGAATCTG	20	20	55.0	55.0	57.8	58.0	9.7	16.2	0.193992	29234	30795	1546-1625	1.000000	90.4
tRNA-Cys-tRNA-Gly_p1	tRNA-Cys-tRNA-Gly	1	CAGATTCGAACTGGGGGATG	GTTCGATTCCCGCTATCCG	20	19	55.0	57.9	58.0	58.2	15.7	0.0	1.230473	30868	31222	430-438	1.000000	82.2
tRNA-Cys-tRNA-Gly_p2	tRNA-Cys-tRNA-Gly	2	CAGATTCGAACTGGGGGATG	GGGTTCGATTCCCGCTATC	20	19	55.0	57.9	58.0	57.8	15.7	9.9	1.253408	30868	31222	432-440	1.000000	82.0
tRNA-Cys-tRNA-Gly_p3	tRNA-Cys-tRNA-Gly	3	CAGATTCGAACTGGGGGATG	GGTTCGATTCCCGCTATCC	20	19	55.0	57.9	58.0	57.8	15.7	0.0	1.253408	30868	31222	431-439	1.000000	82.0
tRNA-Cys-tRNA-Gly_p4	tRNA-Cys-tRNA-Gly	4	AGATTCGAACTGGGGGATGA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.1	58.2	0.0	0.0	1.309939	30868	31222	429-437	1.000000	81.5
tRNA-Cys-tRNA-Gly_p5	tRNA-Cys-tRNA-Gly	5	GGGGGATGAAGGATTTGCAA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.1	58.2	0.0	0.0	1.324845	30868	31222	418-426	1.000000	81.4
tRNA-Ser-tRNA-Thr_p1	tRNA-Ser-tRNA-Thr	1	TCTCCTTGCAATACTCACGC	AGGGCCAGTATGTTTTGTGA	20	20	50.0	45.0	58.0	57.0	0.0	6.2	0.979966	36529	36648	796-796	0.500000	52.2
tRNA-Ser-tRNA-Thr_p2	tRNA-Ser-tRNA-Thr	2	TCGTATCGAATCCTCAGGCT	AGGGCCAGTATGTTTTGTGA	20	20	50.0	45.0	58.0	57.0	0.0	6.2	0.987161	36529	36648	418-418	0.500000	52.1
tRNA-Ser-tRNA-Thr_p3	tRNA-Ser-tRNA-Thr	3	CGGCCTCGATCAGTAAGAAG	AGGGCCAGTATGTTTTGTGA	20	20	55.0	45.0	57.9	57.0	0.0	6.2	1.096735	36529	36648	684-684	0.500000	51.2
tRNA-Ser-tRNA-Thr_p4	tRNA-Ser-tRNA-Thr	4	GGCCTCGATCAGTAAGAAGC	AGGGCCAGTATGTTTTGTGA	20	20	55.0	45.0	58.1	57.0	0.0	6.2	1.110439	36529	36648	683-683	0.500000	51.1
tRNA-Ser-tRNA-Thr_p5	tRNA-Ser-tRNA-Thr	5	ACTACTGACTGGATGAGCCA	AGGGCCAGTATGTTTTGTGA	20	20	50.0	45.0	57.8	57.0	0.0	6.2	1.207905	36529	36648	262-262	0.500000	50.3
tRNA-Ser-tRNA-Leu_p1	tRNA-Ser-tRNA-Leu	1	ATTGACTACCGGCTTGTCAC	ATTGAGTCCGTTGCGTCTAC	20	20	50.0	50.0	57.9	58.0	19.8	0.0	0.102592	45434	47186	1951-1952	1.000000	87.1
tRNA-Ser-tRNA-Leu_p2	tRNA-Ser-tRNA-Leu	2	ACTACCGGCTTGTCACTTTG	ATTGAGTCCGTTGCGTCTAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.128877	45434	47186	1947-1948	1.000000	86.9
tRNA-Ser-tRNA-Leu_p3	tRNA-Ser-tRNA-Leu	3	ATTGACTACCGGCTTGTCAC	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	57.9	58.0	19.8	0.0	0.116117	45434	47186	2002-2003	1.000000	86.7
tRNA-Ser-tRNA-Leu_p4	tRNA-Ser-tRNA-Leu	4	ATTGACTACCGGCTTGTCAC	GTTGAGATGCAACGGAAAGC	20	20	50.0	50.0	57.9	58.0	19.8	0.0	0.125659	45434	47186	2269-2270	1.000000	86.6
tRNA-Ser-tRNA-Leu_p5	tRNA-Ser-tRNA-Leu	5	ACTACCGGCTTGTCACTTTG	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.142402	45434	47186	1998-1999	1.000000	86.5
tRNA-Leu-tRNA-Phe_p1	tRNA-Leu-tRNA-Phe	1	GTGACAGCTCCCAGATTCAG	GAAGAGTGGCTGTCAGTAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.9	0.024552	47698	48045	1451-1468	1.000000	88.8
tRNA-Leu-tRNA-Phe_p2	tRNA-Leu-tRNA-Phe	2	GTAGACGCAACGGACTCAAT	GAAGAGTGGCTGTCAGTAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.9	0.011027	47698	48045	1502-1519	1.000000	88.6
tRNA-Leu-tRNA-Phe_p3	tRNA-Leu-tRNA-Phe	3	GTAGACGCAACGGACTCAAT	GTGGTTTCAATTACCTGCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030642	47698	48045	1465-1482	1.000000	88.6
tRNA-Leu-tRNA-Phe_p4	tRNA-Leu-tRNA-Phe	4	GTAGACGCAACGGACTCAAT	CGTCTAGCTCCTTGTCCAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.010161	47698	48045	1020-1020	0.500000	59.9
tRNA-Leu-tRNA-Phe_p5	tRNA-Leu-tRNA-Phe	5	GTGACAGCTCCCAGATTCAG	CGTCTAGCTCCTTGTCCAAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.023686	47698	48045	969-969	0.500000	59.8
tRNA-Phe-tRNA-Val_p1	tRNA-Phe-tRNA-Val	1	GTTCAAGTCTGGTTCCTGGC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.8	57.9	0.0	0.0	0.836632	48119	50489	2347-2414	1.000000	81.5
tRNA-Phe-tRNA-Val_p2	tRNA-Phe-tRNA-Val	2	GTTCAAGTCTGGTTCCTGGC	CCGAGATTGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.2	0.0	0.0	0.936825	48119	50489	2361-2428	1.000000	80.7
tRNA-Phe-tRNA-Val_p3	tRNA-Phe-tRNA-Val	3	AGTTCAAGTCTGGTTCCTGG	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.1	57.9	0.0	0.0	1.014935	48119	50489	2348-2415	1.000000	80.1
tRNA-Phe-tRNA-Val_p4	tRNA-Phe-tRNA-Val	4	CCAGTTCAAGTCTGGTTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.1	57.9	4.1	0.0	1.014935	48119	50489	2350-2417	1.000000	80.1
tRNA-Phe-tRNA-Val_p5	tRNA-Phe-tRNA-Val	5	GTTCAAGTCTGGTTCCTGGC	TCTGCATGACCGAGATTGTC	20	20	55.0	50.0	58.8	57.7	0.0	0.0	1.060646	48119	50489	2370-2437	1.000000	79.6
tRNA-Ile-tRNA-Arg_p1	tRNA-Ile-tRNA-Arg	1	TGAACCTGTGAATTCGCCAA	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	58.0	57.8	16.1	18.9	0.241642	84201	84986	905-905	0.500000	58.1
tRNA-Ile-tRNA-Arg_p2	tRNA-Ile-tRNA-Arg	2	TTGAACCTGTGAATTCGCCA	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	58.0	57.8	16.1	18.9	0.241642	84201	84986	906-906	0.500000	58.1
tRNA-Ile-tRNA-Arg_p3	tRNA-Ile-tRNA-Arg	3	TAACCGTTCAGCCATGGATG	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.0	57.8	24.3	18.9	0.252865	84201	84986	868-868	0.500000	58.0
tRNA-Ile-tRNA-Arg_p4	tRNA-Ile-tRNA-Arg	4	TTGGGTGCTTTAACCGTTCA	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	57.9	57.8	0.0	18.9	0.270139	84201	84986	878-878	0.500000	57.8
tRNA-Ile-tRNA-Arg_p5	tRNA-Ile-tRNA-Arg	5	AGTTGGGTGCTTTAACCGTT	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	57.9	57.8	0.0	18.9	0.277416	84201	84986	880-880	0.500000	57.8
tRNA-Asn-tRNA-Pro_p1	tRNA-Asn-tRNA-Pro	1	TCATTCTCGTTTGTTTGCGC	AGTTACGTGCGGTATGGAAC	20	20	45.0	50.0	58.0	58.0	0.0	5.4	0.031466	108817	108936	672-672	0.500000	59.7
tRNA-Asn-tRNA-Pro_p2	tRNA-Asn-tRNA-Pro	2	GAAACGTACTTCGGGTTCGA	AGTTACGTGCGGTATGGAAC	20	20	50.0	50.0	57.9	58.0	13.0	5.4	0.050906	108817	108936	534-534	0.500000	59.6
tRNA-Asn-tRNA-Pro_p3	tRNA-Asn-tRNA-Pro	3	AGAAACGTACTTCGGGTTCG	AGTTACGTGCGGTATGGAAC	20	20	50.0	50.0	57.9	58.0	0.0	5.4	0.056524	108817	108936	535-535	0.500000	59.5
tRNA-Asn-tRNA-Pro_p4	tRNA-Asn-tRNA-Pro	4	CTCAGCCCGGTCCATTTTAA	AGTTACGTGCGGTATGGAAC	20	20	50.0	50.0	57.9	58.0	0.0	5.4	0.123213	108817	108936	450-450	0.500000	59.0
tRNA-Asn-tRNA-Pro_p5	tRNA-Asn-tRNA-Pro	5	TCAGCCCGGTCCATTTTAAG	AGTTACGTGCGGTATGGAAC	20	20	50.0	50.0	57.9	58.0	0.0	5.4	0.123213	108817	108936	449-449	0.500000	59.0
tRNA-Pro-tRNA-Leu_p1	tRNA-Pro-tRNA-Leu	1	CGCTACCAAACTGCGCTATA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	9.7	0.0	0.276459	109415	109631	276-281	1.000000	89.2
tRNA-Pro-tRNA-Leu_p2	tRNA-Pro-tRNA-Leu	2	CGCTACCAAACTGCGCTATA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.2	57.8	9.7	0.0	0.337186	109415	109631	269-274	1.000000	88.8
tRNA-Pro-tRNA-Leu_p3	tRNA-Pro-tRNA-Leu	3	CGCTACCAAACTGCGCTATA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.2	57.8	9.7	0.0	0.348477	109415	109631	275-280	1.000000	88.7
tRNA-Pro-tRNA-Leu_p4	tRNA-Pro-tRNA-Leu	4	CGCTACCAAACTGCGCTATA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.2	57.8	9.7	0.0	0.348477	109415	109631	274-279	1.000000	88.7
tRNA-Pro-tRNA-Leu_p5	tRNA-Pro-tRNA-Leu	5	CGCTACCAAACTGCGCTATA	TAGGGCACTGCTTCCTAAGA	20	20	50.0	50.0	58.2	57.8	9.7	0.0	0.399881	109415	109631	288-293	1.000000	88.1
tRNA-Leu-tRNA-Asn_p1	tRNA-Leu-tRNA-Asn	1	GGCCTGCCTTTTTGCATAAG	AAAGGAATCGCATACGGCAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047017	121623	121742	795-795	0.500000	59.6
tRNA-Leu-tRNA-Asn_p2	tRNA-Leu-tRNA-Asn	2	GGCCTGCCTTTTTGCATAAG	AAGGAATCGCATACGGCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047017	121623	121742	794-794	0.500000	59.6
tRNA-Leu-tRNA-Asn_p3	tRNA-Leu-tRNA-Asn	3	GGCCTGCCTTTTTGCATAAG	AGGAATCGCATACGGCATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047017	121623	121742	793-793	0.500000	59.6
tRNA-Leu-tRNA-Asn_p4	tRNA-Leu-tRNA-Asn	4	GGCCTGCCTTTTTGCATAAG	TATGAGGGGGTTCTCTGCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048921	121623	121742	872-872	0.500000	59.6
tRNA-Leu-tRNA-Asn_p5	tRNA-Leu-tRNA-Asn	5	GGCCTGCCTTTTTGCATAAG	TTATGAGGGGGTTCTCTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048921	121623	121742	873-873	0.500000	59.6