primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	TCTATCCCAATGAGCCGTCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.103840	4199	4799	938-1066	1.000000	90.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AGCCGTCTATCGAATCGTTG	20	20	55.0	50.0	58.0	57.9	0.0	1.5	0.136852	4199	4799	926-1054	1.000000	90.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGGATCAGTCGTGGTCTT	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.163373	4199	4799	934-1062	1.000000	89.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	TTGCGGCCATTTTTGAAAGG	20	20	55.0	45.0	58.0	58.1	0.0	2.2	0.139414	4199	4799	1407-1529	1.000000	89.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	TCCGTTGTTCGCATGATTCT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.109042	4199	4799	1462-1476	0.666667	66.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5925	6482	628-669	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5925	6482	639-680	1.000000	89.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	5925	6482	658-699	1.000000	89.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.375253	5925	6482	669-710	1.000000	89.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.597945	5925	6482	659-700	1.000000	87.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CACGAGCCCATATTCTTGCT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.123086	26378	27523	1684-1749	1.000000	89.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GAAAATACCGGGTCTCCACC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.0	58.1	6.3	0.0	0.116232	26378	27523	1889-1954	1.000000	88.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCCGGGCTTTGCAATATTTG	ATAGGAATGGGGAGGTCTCG	20	20	50.0	55.0	58.1	58.0	8.1	0.0	0.078279	26378	27523	1798-1857	0.888889	84.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CACGAGCCCATATTCTTGCT	ATAGGAATGGGGAGGTCTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.057863	26378	27523	1920-1979	0.888889	83.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GAAAATACCGGGTCTCCACC	ATAGGAATGGGGAGGTCTCG	20	20	55.0	55.0	58.0	58.0	6.3	0.0	0.051009	26378	27523	2125-2184	0.888889	83.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.789957	29980	30382	451-474	1.000000	85.7
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.791509	29980	30382	450-473	1.000000	85.7
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.902511	29980	30382	472-495	1.000000	84.8
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.6	57.6	7.1	0.0	0.926188	29980	30382	471-494	1.000000	84.6
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GCGGTGCTCTAACCAATTGA	CGGACTGTAAATTCGTTGGC	20	20	50.0	50.0	58.6	57.5	7.1	0.0	1.071702	29980	30382	467-490	1.000000	83.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30608	31027	507-537	1.000000	88.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	30608	31027	505-535	1.000000	88.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30608	31027	486-516	1.000000	88.9
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30608	31027	484-514	1.000000	88.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	30608	31027	504-534	1.000000	88.4
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	CGTAACCAGTCATCCATAACA	20	21	55.0	42.9	58.1	55.4	7.6	0.0	3.635281	31110	32409	1438-1483	1.000000	60.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	CGTAACCAGTCATCCATAACA	20	21	50.0	42.9	58.1	55.4	0.0	0.0	3.657435	31110	32409	1441-1486	1.000000	60.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	CGTAACCAGTCATCCATAACA	20	21	50.0	42.9	58.3	55.4	0.0	0.0	3.854019	31110	32409	1415-1460	1.000000	58.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	CCGATAAGGGGCTTTTGAGG	CGTAACCAGTCATCCATAACA	20	21	55.0	42.9	58.3	55.4	0.0	0.0	3.890875	31110	32409	1393-1438	1.000000	58.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CGTAACCAGTCATCCATAACATC	20	23	55.0	43.5	58.1	57.1	7.6	0.0	3.971265	31110	32409	1438-1483	1.000000	57.9
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGTCCAGGTAAAGCCCCTAG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.251799	45835	46268	535-567	1.000000	89.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTCCAGGTAAAGCCCCTAGA	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.257252	45835	46268	534-566	1.000000	89.2
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.257252	45835	46268	533-565	1.000000	89.2
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGTCCAGGTAAAGCCCCTAG	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.359553	45835	46268	503-535	1.000000	88.8
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TCCAGGTAAAGCCCCTAGAC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.365005	45835	46268	501-533	1.000000	88.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	46342	47038	532-770	1.000000	88.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	46342	47038	564-802	1.000000	87.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46342	47038	520-758	1.000000	87.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	46342	47038	531-769	1.000000	86.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46342	47038	552-790	1.000000	86.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTTATTGA	20	23	50.0	26.1	58.1	52.2	0.0	13.3	8.822066	48134	48804	727-757	1.000000	49.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CTGGTAGAGCAGAGGACTGA	ATGAAATACAAGATGCTTATTGA	20	23	55.0	26.1	57.9	52.2	2.5	13.3	8.906468	48134	48804	751-781	1.000000	49.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	ATGAAATACAAGATGCTTATTGA	20	23	55.0	26.1	58.0	52.2	10.3	13.3	8.772675	48134	48804	763-793	1.000000	49.1
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AAGGGTGAATGAATGGTCGG	ATGAAATACAAGATGCTTATTGA	20	23	50.0	26.1	57.9	52.2	0.0	13.3	8.898941	48134	48804	781-811	1.000000	48.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGAGAAGATGATGCGCCAAA	ATGAAATACAAGATGCTTATTGA	20	23	45.0	26.1	57.9	52.2	4.2	13.3	8.882550	48134	48804	800-834	1.000000	48.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	AGCGGCCATAAACTTGAGAG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.0	57.8	2.2	0.0	0.227121	50504	51253	1315-1339	1.000000	86.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGTCCTTTGCTATTCGGAG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.242356	50504	51253	897-921	0.888889	83.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AGCGGCCATAAACTTGAGAG	GGAGCATAAACACAAGGGCT	20	20	50.0	50.0	58.0	58.2	2.2	0.0	0.190415	50504	51253	1967-1985	0.888889	80.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	GGAGCATAAACACAAGGGCT	20	20	45.0	50.0	58.1	58.2	2.1	0.0	0.233089	50504	51253	2154-2172	0.888889	79.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGTCCTTTGCTATTCGGAG	GGAGCATAAACACAAGGGCT	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.205649	50504	51253	1549-1566	0.777778	76.1
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.8	52.4	24.9	19.6	8.749230	64557	65703	1193-1309	1.000000	50.7
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.9	52.4	6.4	19.6	8.644221	64557	65703	1270-1386	1.000000	50.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAATAACTAGTTAGAGTAGGCAT	20	23	45.0	30.4	58.2	52.4	0.0	19.6	8.763907	64557	65703	1279-1395	1.000000	50.2
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	58.2	52.4	0.0	19.6	8.776812	64557	65703	1284-1400	1.000000	50.2
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AAATAACTAGTTAGAGTAGGCAT	20	23	50.0	30.4	57.8	52.4	0.0	19.6	8.780238	64557	65703	1278-1394	1.000000	50.2
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	CGGGAGAATCAATGACTGCA	AACCTGAGTTCGGAGAAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039542	107359	107873	882-1002	1.000000	90.5
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	CGGGAGAATCAATGACTGCA	ACCTGAGTTCGGAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039542	107359	107873	881-1001	1.000000	90.5
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	CGGGAGAATCAATGACTGCA	CGGAGAAGGAAGAAATGCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038565	107359	107873	929-992	0.666667	70.6
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CGGGAGAATCAATGACTGCA	TCGGAGAAGGAAGAAATGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038565	107359	107873	930-993	0.666667	70.6
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CGGGAGAATCAATGACTGCA	CCGTAACGACCGAATTGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041722	107359	107873	865-928	0.666667	70.6
ndhF-rpl32_p1	ndhF-rpl32	1	GCAATAGGTCGTGTGAACCA	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	58.2	57.9	0.0	23.0	0.345581	111842	112954	1709-1738	1.000000	87.8
ndhF-rpl32_p2	ndhF-rpl32	2	GCCCATATACGCCGAAGATT	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	57.9	57.9	0.0	23.0	0.243678	111842	112954	1319-1348	0.888889	84.3
ndhF-rpl32_p3	ndhF-rpl32	3	AAAGCCCATATACGCCGAAG	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	58.1	57.9	0.0	23.0	0.269963	111842	112954	1322-1351	0.888889	84.0
ndhF-rpl32_p4	ndhF-rpl32	4	ATGAAGGGGAAATTGGGCAG	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	58.1	57.9	0.0	23.0	0.278851	111842	112954	1951-1967	0.666667	65.0
ndhF-rpl32_p5	ndhF-rpl32	5	GAAGGGGAAATTGGGCAGAT	AAAAGGCTTTTAACGCTGCC	20	20	50.0	45.0	57.8	57.9	0.0	23.0	0.314273	111842	112954	1949-1965	0.666667	64.7
ycf1_p1	ycf1	1	AGTTCCGGACTCAATTCGTG	AATCAACATTGAGGCCTGGG	20	20	50.0	50.0	57.9	58.1	1.8	12.0	0.223657	124401	124550	672-672	0.666667	65.5
ycf1_p2	ycf1	2	CCCATAGTCTCACCCCATCT	AATCAACATTGAGGCCTGGG	20	20	55.0	50.0	57.9	58.1	0.0	12.0	0.252600	124401	124550	561-561	0.666667	65.2
ycf1_p3	ycf1	3	TCGCAATTCTCTACGACGTC	AATCAACATTGAGGCCTGGG	20	20	50.0	50.0	57.8	58.1	2.4	12.0	0.313034	124401	124550	931-931	0.666667	64.8
ycf1_p4	ycf1	4	CGCAATTCTCTACGACGTCT	AATCAACATTGAGGCCTGGG	20	20	50.0	50.0	57.8	58.1	14.7	12.0	0.318901	124401	124550	930-930	0.666667	64.8
ycf1_p5	ycf1	5	ACCCATAGTCTCACCCCATC	AATCAACATTGAGGCCTGGG	20	20	55.0	50.0	58.2	58.1	0.0	12.0	0.335557	124401	124550	562-562	0.666667	64.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	AACCTGAGTTCGGAGAAGGA	CGGGAGAATCAATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039542	129076	129590	882-1002	1.000000	90.5
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	ACCTGAGTTCGGAGAAGGAA	CGGGAGAATCAATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039542	129076	129590	881-1001	1.000000	90.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CGGAGAAGGAAGAAATGCGA	CGGGAGAATCAATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038565	129076	129590	929-992	0.666667	70.6
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TCGGAGAAGGAAGAAATGCG	CGGGAGAATCAATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038565	129076	129590	930-993	0.666667	70.6
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	CCGTAACGACCGAATTGACT	CGGGAGAATCAATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041722	129076	129590	865-928	0.666667	70.6