primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps12_p1 rps12 1 TACGGTTCTGTAGAGTGGCA CAGTACCTCGACGTGACATG 20 20 50.0 55.0 58.1 58.1 0.0 0.0 0.189877 1152 1939 909-909 1.000000 79.0 rps12_p2 rps12 2 TACGGTTCTGTAGAGTGGCA TACAGTACCTCGACGTGACA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.254548 1152 1939 911-911 1.000000 78.5 rps12_p3 rps12 3 TACGGTTCTGTAGAGTGGCA GTACCTCGACGTGACATGAG 20 20 50.0 55.0 58.1 57.8 0.0 0.0 0.261752 1152 1939 907-907 1.000000 78.4 rps12_p4 rps12 4 TACGGTTCTGTAGAGTGGCA AGTACCTCGACGTGACATGA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.268865 1152 1939 908-908 1.000000 78.4 rps12_p5 rps12 5 ATTGAACGAGGAGCCGTATG CAGTACCTCGACGTGACATG 20 20 50.0 55.0 57.8 58.1 0.0 0.0 0.328723 1152 1939 945-945 1.000000 77.9 rpl23_p1 rpl23 1 GAATGGTTAAAGCGCCCAAC CGGTATAGACGCTTATGGCC 20 20 50.0 55.0 58.0 58.0 2.3 0.0 0.017031 12689 12955 683-683 1.000000 80.4 rpl23_p2 rpl23 2 GAATGGTTAAAGCGCCCAAC CTTAGATCCGGCTCTACCCA 20 20 50.0 55.0 58.0 58.0 2.3 14.5 0.012402 12689 12955 1131-1131 1.000000 80.3 rpl23_p3 rpl23 3 GAATGGTTAAAGCGCCCAAC TTATGGCCTCCCCCTCTATG 20 20 50.0 55.0 58.0 58.0 2.3 3.3 0.053993 12689 12955 671-671 1.000000 80.2 rpl23_p4 rpl23 4 CCAAATTCGGGGGTCAAAGA CTTAGATCCGGCTCTACCCA 20 20 50.0 55.0 58.1 58.0 0.0 14.5 0.085330 12689 12955 1472-1474 1.000000 79.7 rpl23_p5 rpl23 5 TCCAAATTCGGGGGTCAAAG CTTAGATCCGGCTCTACCCA 20 20 50.0 55.0 58.1 58.0 0.0 14.5 0.085330 12689 12955 1473-1475 1.000000 79.7 rpl23-rpl2_p1 rpl23-rpl2 1 TCATTACGCTTCAATCGGGT TGCTCGGGGTAGAAGTTTTG 20 20 45.0 50.0 57.3 57.8 0.0 0.0 0.862913 12956 13021 144-144 1.000000 73.7 rpl23-rpl2_p2 rpl23-rpl2 2 TCATTACGCTTCAATCGGGT GCTCGGGGTAGAAGTTTTGT 20 20 45.0 50.0 57.3 57.8 0.0 0.0 0.864433 12956 13021 143-143 1.000000 73.6 rpl23-rpl2_p3 rpl23-rpl2 3 TCATTACGCTTCAATCGGGT GTGCTCGGGGTAGAAGTTTT 20 20 45.0 50.0 57.3 57.8 0.0 0.0 0.864433 12956 13021 145-145 1.000000 73.6 rpl23-rpl2_p4 rpl23-rpl2 4 TCATTACGCTTCAATCGGGT CAACGACGCTGTCCATAGAT 20 20 45.0 50.0 57.3 57.8 0.0 0.0 0.893840 12956 13021 217-217 1.000000 73.2 rpl23-rpl2_p5 rpl23-rpl2 5 TCATTACGCTTCAATCGGGT ATTGTCTACGGCTCCATTGC 20 20 45.0 50.0 57.3 58.3 0.0 0.0 1.015582 12956 13021 167-167 1.000000 72.4 trnG-UCC_p1 trnG-UCC 1 TCGACAAAAGGTTCAGCTCA TGGATTGTACGCGAGATGAG 20 20 45.0 50.0 57.4 57.5 0.0 15.9 1.105045 27348 28106 876-876 1.000000 71.3 trnG-UCC_p2 trnG-UCC 2 AGCTCGACAAAAGGTTCAGC TGGATTGTACGCGAGATGAG 20 20 50.0 50.0 58.8 57.5 0.0 15.9 1.261696 27348 28106 879-879 1.000000 70.0 trnG-UCC_p3 trnG-UCC 3 GCTCGACAAAAGGTTCAGCT TGGATTGTACGCGAGATGAG 20 20 50.0 50.0 58.8 57.5 0.0 15.9 1.261696 27348 28106 878-878 1.000000 70.0 trnG-UCC_p4 trnG-UCC 4 TCGACAAAAGGTTCAGCTCA GGATTGTACGCGAGATGAGA 20 20 45.0 50.0 57.4 57.2 0.0 15.9 1.389338 27348 28106 875-875 1.000000 69.0 trnG-UCC_p5 trnG-UCC 5 GCTCGACAAAAGGTTCAGCT GGATTGTACGCGAGATGAGA 20 20 50.0 50.0 58.8 57.2 0.0 15.9 1.545988 27348 28106 877-877 1.000000 67.7 trnR-UCU-atpA_p1 trnR-UCU-atpA 1 ATCGGAAAAGCGTCCATTGT GAGGAAGCAGAAGCCCTTTT 20 20 45.0 50.0 58.2 58.1 5.2 0.0 0.262278 28319 28419 257-257 1.000000 79.5 trnR-UCU-atpA_p2 trnR-UCU-atpA 2 CGGAAAAGCGTCCATTGTCT GAGGAAGCAGAAGCCCTTTT 20 20 50.0 50.0 58.8 58.1 5.2 0.0 0.926758 28319 28419 255-255 1.000000 74.2 trnR-UCU-atpA_p3 trnR-UCU-atpA 3 TCGGAAAAGCGTCCATTGTC GAGGAAGCAGAAGCCCTTTT 20 20 50.0 50.0 58.8 58.1 5.2 0.0 0.930142 28319 28419 256-256 1.000000 74.1 trnR-UCU-atpA_p4 trnR-UCU-atpA 4 ATCGGAAAAGCGTCCATTGT TTACTGAGGAAGCAGAAGCC 20 20 45.0 50.0 58.2 57.2 5.2 0.0 0.948947 28319 28419 262-262 1.000000 73.9 trnR-UCU-atpA_p5 trnR-UCU-atpA 5 AATCGGAAAAGCGTCCATTG GAGGAAGCAGAAGCCCTTTT 20 20 45.0 50.0 57.1 58.1 5.2 0.0 1.003161 28319 28419 258-258 1.000000 73.5 atpA-atpF_p1 atpA-atpF 1 CAATGCCGTCACCTACTTGA GCATGTTTGGTGCCATGAAA 20 20 50.0 45.0 57.9 57.8 0.0 0.0 0.260128 29944 30012 222-222 1.000000 79.4 atpA-atpF_p2 atpA-atpF 2 ATGCCGTCACCTACTTGAAG GCATGTTTGGTGCCATGAAA 20 20 50.0 45.0 57.6 57.8 0.0 0.0 0.553482 29944 30012 220-220 1.000000 77.0 atpA-atpF_p3 atpA-atpF 3 TGCTAATTTCGTCTGCTCGA GCATGTTTGGTGCCATGAAA 20 20 45.0 45.0 57.4 57.8 0.0 0.0 0.814351 29944 30012 135-135 1.000000 75.9 atpA-atpF_p4 atpA-atpF 4 AATGCCGTCACCTACTTGAA GCATGTTTGGTGCCATGAAA 20 20 45.0 45.0 57.1 57.8 0.0 0.0 1.018084 29944 30012 221-221 1.000000 73.3 atpA-atpF_p5 atpA-atpF 5 TGCCGTCACCTACTTGAAGT GCATGTTTGGTGCCATGAAA 20 20 50.0 45.0 59.0 57.8 2.4 0.0 1.121415 29944 30012 219-219 1.000000 72.5 rpoC1-rpoB_p1 rpoC1-rpoB 1 TGGCCCAAGCACTTATTTGT ACGATCTTTGGCTCTGGAAC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.108847 41700 41725 175-175 1.000000 79.6 rpoC1-rpoB_p2 rpoC1-rpoB 2 TGTGGCCCAAGCACTTATTT ACGATCTTTGGCTCTGGAAC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.108847 41700 41725 177-177 1.000000 79.6 rpoC1-rpoB_p3 rpoC1-rpoB 3 TTGTGGCCCAAGCACTTATT ACGATCTTTGGCTCTGGAAC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.108847 41700 41725 178-178 1.000000 79.6 rpoC1-rpoB_p4 rpoC1-rpoB 4 TTTGTGGCCCAAGCACTTAT ACGATCTTTGGCTCTGGAAC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.108847 41700 41725 179-179 1.000000 79.6 rpoC1-rpoB_p5 rpoC1-rpoB 5 GGTAGGATTTTTGTGGCCCA ACGATCTTTGGCTCTGGAAC 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.168969 41700 41725 188-188 1.000000 79.1 accD_p1 accD 1 TTCTGGATATTCAGCACGCC CTGCGGACTCTTCTTCCTTG 20 20 50.0 55.0 58.0 58.3 6.1 0.0 0.326841 79277 79396 311-311 1.000000 78.7 accD_p2 accD 2 TTCTGGATATTCAGCACGCC TCTGCGGACTCTTCTTCCTT 20 20 50.0 50.0 58.0 58.4 6.1 0.0 0.412667 79277 79396 312-312 1.000000 78.0 accD_p3 accD 3 TTCTGGATATTCAGCACGCC TTCTGCGGACTCTTCTTCCT 20 20 50.0 50.0 58.0 58.4 6.1 0.0 0.412667 79277 79396 313-313 1.000000 78.0 accD_p4 accD 4 CACGCCGAATGGATGGTATT CTGCGGACTCTTCTTCCTTG 20 20 50.0 55.0 58.4 58.3 0.0 0.0 0.687204 79277 79396 297-297 1.000000 75.8 accD_p5 accD 5 TTCTGGATATTCAGCACGCC ATTCTGCGGACTCTTCTTCC 20 20 50.0 50.0 58.0 57.3 6.1 0.0 0.728596 79277 79396 314-314 1.000000 75.4 accD-psaI_p1 accD-psaI 1 TACTTGAGCTGCACGGTTTT CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 58.0 58.0 1.0 26.9 0.065210 80478 81098 744-744 1.000000 79.9 accD-psaI_p2 accD-psaI 2 TTACTTGAGCTGCACGGTTT CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 58.0 58.0 1.0 26.9 0.065210 80478 81098 745-745 1.000000 79.9 accD-psaI_p3 accD-psaI 3 TACTTGAGCTGCACGGTTTT AAGAAGCCATTGCAATTGCC 20 20 45.0 45.0 58.0 57.9 1.0 26.8 0.141744 80478 81098 750-750 1.000000 79.3 accD-psaI_p4 accD-psaI 4 TTACTTGAGCTGCACGGTTT AAGAAGCCATTGCAATTGCC 20 20 45.0 45.0 58.0 57.9 1.0 26.8 0.141744 80478 81098 751-751 1.000000 79.3 accD-psaI_p5 accD-psaI 5 TCGATCCAGTCTTACCACGT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.2 58.0 0.0 26.9 0.196724 80478 81098 792-792 1.000000 78.8 petA-psbJ_p1 petA-psbJ 1 ATTGCGCGTACAAGGTCTTT ACTGGCATTCTTGTGATCGT 20 20 45.0 45.0 58.5 57.5 8.4 0.0 0.960157 84964 86004 1231-1234 1.000000 72.7 petA-psbJ_p2 petA-psbJ 2 ATTGCGCGTACAAGGTCTTT CGTATTCTGGATTGGGCTCA 20 20 45.0 50.0 58.5 57.4 8.4 0.0 1.100843 84964 86004 1185-1188 1.000000 71.6 petA-psbJ_p3 petA-psbJ 3 ATTGCGCGTACAAGGTCTTT ATTCTGGATTGGGCTCATCC 20 20 45.0 50.0 58.5 57.3 8.4 7.8 1.152487 84964 86004 1182-1185 1.000000 71.2 petA-psbJ_p4 petA-psbJ 4 TTGCGCGTACAAGGTCTTTT ACTGGCATTCTTGTGATCGT 20 20 45.0 45.0 58.7 57.5 8.4 0.0 1.178143 84964 86004 1230-1233 1.000000 71.0 petA-psbJ_p5 petA-psbJ 5 TTGCGCGTACAAGGTCTTTT CGTATTCTGGATTGGGCTCA 20 20 45.0 50.0 58.7 57.4 8.4 0.0 1.318829 84964 86004 1184-1187 1.000000 69.9 rps18_p1 rps18 1 CGTTTGTGGATCACTCGGAT CTAGTCGGTTGGAATTGCGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.014728 89547 89969 1271-1307 1.000000 81.7 rps18_p2 rps18 2 CGTTTGTGGATCACTCGGAT AGGAGGTTAGGTTGATGGCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.037557 89547 89969 1412-1448 1.000000 81.3 rps18_p3 rps18 3 CGTTTGTGGATCACTCGGAT GGAGGTTAGGTTGATGGCTG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.054268 89547 89969 1411-1447 1.000000 81.2 rps18_p4 rps18 4 CGTTTGTGGATCACTCGGAT GGTTAGGTTGATGGCTGAGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.054268 89547 89969 1408-1444 1.000000 81.2 rps18_p5 rps18 5 CGTTTGTGGATCACTCGGAT GTTAGGTTGATGGCTGAGGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.054268 89547 89969 1407-1443 1.000000 81.2 trnR-ACG-trnN-GUU_p1 trnR-ACG-trnN-GUU 1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.8 58.0 18.9 0.0 0.218238 109230 109851 739-763 1.000000 80.8 trnR-ACG-trnN-GUU_p2 trnR-ACG-trnN-GUU 2 CAGAGGATTAGAGCACGTGG GATCGAGTCTCCCGTTGTTC 20 20 55.0 55.0 57.8 58.1 18.9 0.0 0.305219 109230 109851 750-774 1.000000 80.1 trnR-ACG-trnN-GUU_p3 trnR-ACG-trnN-GUU 3 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 20 20 55.0 50.0 57.8 58.1 18.9 0.0 0.311620 109230 109851 780-804 1.000000 79.9 trnR-ACG-trnN-GUU_p4 trnR-ACG-trnN-GUU 4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 20 20 55.0 50.0 57.8 58.2 18.9 18.9 0.372539 109230 109851 690-714 1.000000 79.8 trnR-ACG-trnN-GUU_p5 trnR-ACG-trnN-GUU 5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 20 20 55.0 50.0 57.8 57.8 18.9 0.0 0.410108 109230 109851 779-803 1.000000 79.1 ndhA-trnL-UAG_p1 ndhA-trnL-UAG 1 TTTTAGGTGGTCTACGGGCT CTCGCACTGCTTCCTAAGAG 20 20 50.0 55.0 58.4 58.1 0.0 0.0 0.431966 120168 120986 964-992 1.000000 76.7 ndhA-trnL-UAG_p2 ndhA-trnL-UAG 2 TTTTAGGTGGTCTACGGGCT CTCTCGCACTGCTTCCTAAG 20 20 50.0 55.0 58.4 58.1 0.0 0.0 0.431966 120168 120986 966-994 1.000000 76.7 ndhA-trnL-UAG_p3 ndhA-trnL-UAG 3 TTTTAGGTGGTCTACGGGCT TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.4 57.9 0.0 0.0 0.476161 120168 120986 953-981 1.000000 76.4 ndhA-trnL-UAG_p4 ndhA-trnL-UAG 4 TTTTAGGTGGTCTACGGGCT AGCAGCGTGTCTACCAATTT 20 20 50.0 45.0 58.4 57.8 0.0 0.0 0.536888 120168 120986 946-974 1.000000 75.9 ndhA-trnL-UAG_p5 ndhA-trnL-UAG 5 TTTTAGGTGGTCTACGGGCT CTAAGAGCAGCGTGTCTACC 20 20 50.0 55.0 58.4 57.8 0.0 0.0 0.548179 120168 120986 951-979 1.000000 75.8 trnL-UAG-ccsA_p1 trnL-UAG-ccsA 1 CTCTTAGGAAGCAGTGCGAG TGGAAACCATTGAAAAGGAA 20 20 55.0 35.0 58.1 53.1 10.0 0.0 4.946417 121067 121180 222-228 1.000000 41.5 trnL-UAG-ccsA_p2 trnL-UAG-ccsA 2 CTTAGGAAGCAGTGCGAGAG TGGAAACCATTGAAAAGGAA 20 20 55.0 35.0 58.1 53.1 10.0 0.0 4.946417 121067 121180 220-226 1.000000 41.5 trnL-UAG-ccsA_p3 trnL-UAG-ccsA 3 TAGACACGCTGCTCTTAGGA TGGAAACCATTGAAAAGGAA 20 20 50.0 35.0 57.9 53.1 0.0 0.0 4.990612 121067 121180 233-239 1.000000 41.1 trnL-UAG-ccsA_p4 trnL-UAG-ccsA 4 CTCTTAGGAAGCAGTGCGAG TGGAAACCATTGAAAAGGAAA 20 21 55.0 33.3 58.1 54.1 10.0 0.0 5.025571 121067 121180 222-228 1.000000 41.1 trnL-UAG-ccsA_p5 trnL-UAG-ccsA 5 CTTAGGAAGCAGTGCGAGAG TGGAAACCATTGAAAAGGAAA 20 21 55.0 33.3 58.1 54.1 10.0 0.0 5.025571 121067 121180 220-226 1.000000 41.1 trnN-GUU-trnR-ACG_p1 trnN-GUU-trnR-ACG 1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 20 20 55.0 55.0 58.0 57.8 0.0 18.9 0.218238 138535 139156 739-763 1.000000 80.8 trnN-GUU-trnR-ACG_p2 trnN-GUU-trnR-ACG 2 GATCGAGTCTCCCGTTGTTC CAGAGGATTAGAGCACGTGG 20 20 55.0 55.0 58.1 57.8 0.0 18.9 0.305219 138535 139156 750-774 1.000000 80.1 trnN-GUU-trnR-ACG_p3 trnN-GUU-trnR-ACG 3 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 58.1 57.8 0.0 18.9 0.311620 138535 139156 780-804 1.000000 79.9 trnN-GUU-trnR-ACG_p4 trnN-GUU-trnR-ACG 4 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 58.2 57.8 18.9 18.9 0.372539 138535 139156 690-714 1.000000 79.8 trnN-GUU-trnR-ACG_p5 trnN-GUU-trnR-ACG 5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 57.8 57.8 0.0 18.9 0.410108 138535 139156 779-803 1.000000 79.1