primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	AGCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	22	22	36.4	31.8	55.7	53.0	14.4	25.1	11.299175	5572	5691	278-476	1.000000	40.6
rps16_p2	rps16	2	AAGCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	23	22	34.8	31.8	56.4	53.0	0.0	25.1	11.586413	5572	5691	279-477	1.000000	40.6
rps16_p3	rps16	3	AGCTTGTTACGTTAAGAAGACAA	TCTTATATGCATGCTTATACGT	23	22	34.8	31.8	56.4	53.0	14.4	25.1	11.586413	5572	5691	278-476	1.000000	40.6
rps16_p4	rps16	4	AGCTTGTTACGTTAAGAAGACA	ATCTTATATGCATGCTTATACGT	22	23	36.4	30.4	55.7	53.6	14.4	25.1	11.673685	5572	5691	279-477	1.000000	40.6
rps16_p5	rps16	5	GCTTGTTACGTTAAGAAGACA	TCTTATATGCATGCTTATACGT	21	22	38.1	31.8	54.2	53.0	14.4	25.1	11.799926	5572	5691	277-475	1.000000	40.6
trnQ-UUG-trnG-UCC_p1	trnQ-UUG-trnG-UCC	1	GTCCAGTCATTCGGAGGTTC	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.041378	11274	13355	2229-2229	1.000000	79.7
trnQ-UUG-trnG-UCC_p2	trnQ-UUG-trnG-UCC	2	GTCCAGTCATTCGGAGGTTC	CGTTAGCTTGGAAGGCTAGG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.070215	11274	13355	2226-2226	1.000000	79.5
trnQ-UUG-trnG-UCC_p3	trnQ-UUG-trnG-UCC	3	GAGGTTCGAATCCTTCCGTC	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	58.1	58.0	16.9	0.0	0.105939	11274	13355	2216-2216	1.000000	79.2
trnQ-UUG-trnG-UCC_p4	trnQ-UUG-trnG-UCC	4	GAGGTTCGAATCCTTCCGTC	CGTTAGCTTGGAAGGCTAGG	20	20	55.0	55.0	58.1	58.1	16.9	0.0	0.134776	11274	13355	2213-2213	1.000000	79.0
trnQ-UUG-trnG-UCC_p5	trnQ-UUG-trnG-UCC	5	GTCCAGTCATTCGGAGGTTC	GTTAGCTTGGAAGGCTAGGG	20	20	55.0	55.0	58.0	57.7	0.0	0.0	0.345228	11274	13355	2225-2225	1.000000	77.3
rpoC2_p1	rpoC2	1	TTATATGGGGCTAGGGGTCG	TCCCGAGGAAGTGCATATCT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.141120	22971	23090	852-900	1.000000	84.3
rpoC2_p2	rpoC2	2	ATGTCGGGTAAGTGAGGGAA	TCCCGAGGAAGTGCATATCT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.141120	22971	23090	1261-1309	1.000000	83.9
rpoC2_p3	rpoC2	3	TGTCGGGTAAGTGAGGGAAT	TCCCGAGGAAGTGCATATCT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.141120	22971	23090	1260-1308	1.000000	83.9
rpoC2_p4	rpoC2	4	TTATATGGGGCTAGGGGTCG	TTTCATTCCCGAGGAAGTGC	20	20	55.0	50.0	58.1	58.2	0.0	10.5	0.231799	22971	23090	858-906	1.000000	83.6
rpoC2_p5	rpoC2	5	TGTCGGGTAAGTGAGGGAAT	TTTCATTCCCGAGGAAGTGC	20	20	50.0	50.0	58.1	58.2	0.0	10.5	0.231799	22971	23090	1266-1314	1.000000	83.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGCGAGAATATTGACTTCCA	TATTGTACGGCTCATGGGGA	20	20	40.0	50.0	54.5	58.2	0.0	0.0	3.723530	35878	36740	974-974	1.000000	50.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TGCGAGAATATTGACTTCCA	GCTCATGGGGACTGTAGTTC	20	20	40.0	55.0	54.5	57.7	0.0	0.0	3.837076	35878	36740	965-965	1.000000	49.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGCGAGAATATTGACTTCCA	AGTTCAATTGGTCAGAGCGT	20	20	40.0	45.0	54.5	57.4	0.0	5.4	4.070377	35878	36740	950-950	1.000000	47.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCGTCG	20	20	40.0	50.0	54.5	58.6	0.0	5.4	4.091098	35878	36740	948-948	1.000000	47.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGCGAGAATATTGACTTCCAT	TATTGTACGGCTCATGGGGA	21	20	38.1	50.0	55.1	58.2	0.0	0.0	4.106257	35878	36740	974-974	1.000000	47.2
ycf3_p1	ycf3	1	CTTGATTGGACCCTCTCAGG	TATGAGATCGTTTTCCCGGG	20	20	55.0	50.0	57.3	57.1	0.0	16.9	1.609044	49508	51493	2128-2128	1.000000	67.2
ycf3_p2	ycf3	2	CTTGATTGGACCCTCTCAGG	TTATGAGATCGTTTTCCCGGG	20	21	55.0	47.6	57.3	57.8	0.0	16.9	1.894498	49508	51493	2129-2129	1.000000	64.9
ycf3_p3	ycf3	3	CTTGATTGGACCCTCTCAGG	ATGAGATCGTTTTCCCGGG	20	19	55.0	52.6	57.3	57.2	0.0	16.9	2.486963	49508	51493	2127-2127	1.000000	60.1
ycf3_p4	ycf3	4	CTTGATTGGACCCTCTCAGG	ATGAGATCGTTTTCCCGGGG	20	20	55.0	55.0	57.3	59.8	0.0	23.5	2.525933	49508	51493	2127-2127	1.000000	59.8
ycf3_p5	ycf3	5	TCTTGATTGGACCCTCTCAG	TATGAGATCGTTTTCCCGGG	20	20	50.0	50.0	56.2	57.1	0.0	16.9	2.658121	49508	51493	2129-2129	1.000000	58.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	53777	56369	2686-2688	1.000000	79.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	53777	56369	2694-2696	1.000000	78.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	53777	56369	2673-2675	1.000000	78.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	53777	56369	2681-2683	1.000000	77.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAATGCGATGCTCTAACCCC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.324286	53777	56369	2645-2647	1.000000	77.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	GTTGGATCGGTTGGCCTTTA	20	20	50.0	50.0	58.1	58.2	0.0	3.3	0.222916	57597	58251	760-760	1.000000	78.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TTGGATCGGTTGGCCTTTAC	20	20	50.0	50.0	58.1	58.2	0.0	3.3	0.222916	57597	58251	759-759	1.000000	78.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	GATCGGTTGGCCTTTACGTA	20	20	50.0	50.0	58.1	57.7	0.0	3.3	0.350307	57597	58251	756-756	1.000000	77.3
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AGTCATCCGCGACTTAAACG	20	20	50.0	50.0	58.1	58.4	0.0	8.2	0.426280	57597	58251	797-797	1.000000	76.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TCGGGATAGCTCAGTTGGTA	TTGGATCGGTTGGCCTTTAC	20	20	50.0	50.0	57.6	58.2	0.0	3.3	0.618429	57597	58251	797-797	1.000000	75.2
accD-psaI_p1	accD-psaI	1	CACGGGTTCCTTCCTTTGAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	69322	70613	1401-1401	1.000000	79.8
accD-psaI_p2	accD-psaI	2	CACGGGTTCCTTCCTTTGAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	69322	70613	1407-1407	1.000000	79.1
accD-psaI_p3	accD-psaI	3	GTTATTTCAGCTCCACGGGT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.136428	69322	70613	1414-1414	1.000000	79.1
accD-psaI_p4	accD-psaI	4	CCTCATCGTACCACGGAATC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	57.9	58.0	0.0	26.9	0.162668	69322	70613	1456-1456	1.000000	78.9
accD-psaI_p5	accD-psaI	5	GTTATTTCAGCTCCACGGGT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	0.0	26.8	0.212962	69322	70613	1420-1420	1.000000	78.5
psbE-petL_p1	psbE-petL	1	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	75521	76709	1349-1349	1.000000	74.1
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	75521	76709	1348-1348	1.000000	74.1
psbE-petL_p3	psbE-petL	3	AATAACCAACCCGCGATGAA	AAGCAGCCAGTAGAAAACCA	20	20	45.0	45.0	57.9	57.3	8.2	0.0	0.773774	75521	76709	1342-1342	1.000000	73.9
psbE-petL_p4	psbE-petL	4	AATAACCAACCCGCGATGAA	AGCAGCCAGTAGAAAACCAA	20	20	45.0	45.0	57.9	57.3	8.2	0.0	0.773774	75521	76709	1341-1341	1.000000	73.9
psbE-petL_p5	psbE-petL	5	AACCAACCCGCGATGAATAG	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	58.3	57.3	8.2	0.0	0.993253	75521	76709	1338-1338	1.000000	72.1
rpoA_p1	rpoA	1	AGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	21	20	33.3	50.0	54.1	57.9	1.7	0.0	4.923789	89628	90662	1144-1144	1.000000	40.7
rpoA_p2	rpoA	2	AAGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	22	20	31.8	50.0	55.0	57.9	1.7	0.0	5.093508	89628	90662	1145-1145	1.000000	40.1
rpoA_p3	rpoA	3	AGAAAAAGGAATTGCAAAGGA	ACCCCCTAAAAGAAGGCGTA	21	20	33.3	50.0	54.1	58.3	1.7	0.0	5.197648	89628	90662	1115-1115	1.000000	40.1
rpoA_p4	rpoA	4	AGAAAAAGGAATTGCAAAGGAT	GCAACCCCTATGCCACATAA	22	20	31.8	50.0	54.8	57.9	1.7	0.0	5.312470	89628	90662	1144-1144	1.000000	40.1
rpoA_p5	rpoA	5	AAAGAAAAAGGAATTGCAAAGGA	GCAACCCCTATGCCACATAA	23	20	30.4	50.0	55.7	57.9	1.7	0.0	5.335661	89628	90662	1146-1146	1.000000	40.1
rpl22-rps19_p1	rpl22-rps19	1	TTTGACTTCTACGCCCGATG	ACCTACTCGAACTTTTGGCG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.212577	95995	97187	1254-1290	1.000000	82.5
rpl22-rps19_p2	rpl22-rps19	2	AACTTTGACTTCTACGCCCG	ACCTACTCGAACTTTTGGCG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.415988	95995	97187	1257-1293	1.000000	80.9
rpl22-rps19_p3	rpl22-rps19	3	GCCCGCGAATTTGATCAATC	ACCTACTCGAACTTTTGGCG	20	20	50.0	50.0	58.2	58.2	8.2	0.0	0.449211	95995	97187	1321-1357	1.000000	80.6
rpl22-rps19_p4	rpl22-rps19	4	TGACTTCTACGCCCGATGTA	ACCTACTCGAACTTTTGGCG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.454064	95995	97187	1252-1288	1.000000	80.6
rpl22-rps19_p5	rpl22-rps19	5	CTTTGGGCTTTGAAAGGGGA	ACCTACTCGAACTTTTGGCG	20	20	50.0	50.0	58.3	58.2	7.1	0.0	0.492209	95995	97187	1298-1334	1.000000	80.2
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	CTACGGCCAATCGGTTAACA	CAGAATGAGCTCAGGAACCC	20	20	50.0	55.0	58.0	58.0	8.1	23.0	0.048467	122258	123061	904-962	1.000000	83.6
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	TACGGCCAATCGGTTAACAG	CAGAATGAGCTCAGGAACCC	20	20	50.0	55.0	58.0	58.0	8.1	23.0	0.048467	122258	123061	903-961	1.000000	83.6
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	ATTCGAACCTACGGCCAATC	CAGAATGAGCTCAGGAACCC	20	20	50.0	55.0	58.1	58.0	2.2	23.0	0.086065	122258	123061	912-970	1.000000	83.3
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GATTCGAACCTACGGCCAAT	CAGAATGAGCTCAGGAACCC	20	20	50.0	55.0	58.1	58.0	2.2	23.0	0.086065	122258	123061	913-971	1.000000	83.3
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GTAGGATTCGAACCTACGGC	CAGAATGAGCTCAGGAACCC	20	20	55.0	55.0	57.9	58.0	26.6	23.0	0.166811	122258	123061	917-975	1.000000	82.7
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGGAGTCCAAA	ACGAAAAAGCTTTAACGGCG	20	20	50.0	45.0	58.0	58.0	12.9	28.2	0.045658	133902	135251	1538-1539	1.000000	79.7
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAGGAGTCCAAACCCTAT	ACGAAAAAGCTTTAACGGCG	20	20	50.0	45.0	58.0	58.0	12.9	28.2	0.054327	133902	135251	1532-1533	1.000000	79.6
ndhF-rpl32_p3	ndhF-rpl32	3	TCCAAACCCTATTGCCATCG	ACGAAAAAGCTTTAACGGCG	20	20	50.0	45.0	57.9	58.0	0.0	28.2	0.078249	133902	135251	1524-1525	1.000000	79.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGAGTCCAAACCCTATTGCC	ACGAAAAAGCTTTAACGGCG	20	20	55.0	45.0	58.2	58.0	3.6	28.2	0.262947	133902	135251	1528-1529	1.000000	77.9
ndhF-rpl32_p5	ndhF-rpl32	5	CCAAACCCTATTGCCATCGT	ACGAAAAAGCTTTAACGGCG	20	20	50.0	45.0	58.2	58.0	0.0	28.2	0.264207	133902	135251	1523-1524	1.000000	77.9
ccsA_p1	ccsA	1	AATAGAAAAGGAGCGCAAGA	ACTGAGCGATGAAATGGAGT	20	20	40.0	45.0	55.1	56.9	11.5	0.0	4.005755	136206	137174	1169-1169	1.000000	48.0
ccsA_p2	ccsA	2	AATAGAAAAGGAGCGCAAGA	CGTGAGAGTGATGTAGAGGG	20	20	40.0	55.0	55.1	56.9	11.5	0.0	4.083762	136206	137174	1111-1111	1.000000	47.4
ccsA_p3	ccsA	3	AAATAGAAAAGGAGCGCAAGA	ACTGAGCGATGAAATGGAGT	21	20	38.1	45.0	55.9	56.9	11.5	0.0	4.191497	136206	137174	1170-1170	1.000000	46.5
ccsA_p4	ccsA	4	AGAAAAGGAGCGCAAGATATTC	ACTGAGCGATGAAATGGAGT	22	20	40.9	45.0	56.8	56.9	11.5	0.0	4.220273	136206	137174	1166-1166	1.000000	46.3
ccsA_p5	ccsA	5	GAAAAGGAGCGCAAGATATTCT	ACTGAGCGATGAAATGGAGT	22	20	40.9	45.0	56.8	56.9	11.5	0.0	4.220273	136206	137174	1165-1165	1.000000	46.3
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	CAGAATGAGCTCAGGAACCC	CTACGGCCAATCGGTTAACA	20	20	55.0	50.0	58.0	58.0	23.0	8.1	0.048467	152631	153434	904-962	1.000000	83.6
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	CAGAATGAGCTCAGGAACCC	TACGGCCAATCGGTTAACAG	20	20	55.0	50.0	58.0	58.0	23.0	8.1	0.048467	152631	153434	903-961	1.000000	83.6
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CAGAATGAGCTCAGGAACCC	ATTCGAACCTACGGCCAATC	20	20	55.0	50.0	58.0	58.1	23.0	2.2	0.086065	152631	153434	912-970	1.000000	83.3
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	CAGAATGAGCTCAGGAACCC	GATTCGAACCTACGGCCAAT	20	20	55.0	50.0	58.0	58.1	23.0	2.2	0.086065	152631	153434	913-971	1.000000	83.3
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	CAGAATGAGCTCAGGAACCC	GTAGGATTCGAACCTACGGC	20	20	55.0	55.0	58.0	57.9	23.0	26.6	0.166811	152631	153434	917-975	1.000000	82.7
ycf2_p1	ycf2	1	TGCATCGAACACTGGTTGAA	GATCCACTGCGGGAATGATT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066711	173988	174107	796-846	1.000000	83.6
ycf2_p2	ycf2	2	TCATGGATCTCTCGGTTCGA	GATCCACTGCGGGAATGATT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079910	173988	174107	614-664	1.000000	83.5
ycf2_p3	ycf2	3	TCATGGATCTCTCGGTTCGA	CAGGCAATCGGAAAAGCAAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.066084	173988	174107	1076-1126	1.000000	82.7
ycf2_p4	ycf2	4	TGCATCGAACACTGGTTGAA	CAGGCAATCGGAAAAGCAAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.052885	173988	174107	1258-1308	1.000000	82.4
ycf2_p5	ycf2	5	CGGCCCATTCGTATTCGTAT	CAGGCAATCGGAAAAGCAAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.087849	173988	174107	1124-1174	1.000000	82.4