primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATGATCGTGAGCAAATGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.9	58.2	16.8	17.1	0.283768	3530	4236	838-839	1.000000	78.1
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCATGATCGTGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.9	58.2	16.8	17.1	0.283768	3530	4236	837-838	1.000000	78.1
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCATGATCGTGAGCAAATGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.9	57.5	16.8	0.0	0.576105	3530	4236	851-852	1.000000	75.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCATGATCGTGAGCAAATGC	TTCGGGTTGCTAACTCAACG	20	20	50.0	50.0	57.9	58.5	16.8	0.0	0.610989	3530	4236	854-855	1.000000	75.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGAAACCATGATCGTGAGCA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.5	58.2	16.8	17.1	0.639403	3530	4236	842-843	1.000000	75.3
atpH-atpI_p1	atpH-atpI	1	TCCGATAGAAGCAAGCCCTA	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.341112	14103	15164	1232-1235	1.000000	78.0
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	0.0	0.0	0.362752	14103	15164	1193-1196	1.000000	77.9
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.371251	14103	15164	1251-1254	1.000000	77.8
atpH-atpI_p4	atpH-atpI	4	TCCAGGTCCGATAGAAGCAA	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.398331	14103	15164	1238-1241	1.000000	77.6
atpH-atpI_p5	atpH-atpI	5	GATAGAAGCAAGCCCTACGG	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.415315	14103	15164	1229-1232	1.000000	77.5
rpoC1-trnC-GCA_p1	rpoC1-trnC-GCA	1	ACCCAGTTCCAACAATGCAT	ATTCAGGAAGAACAGGTGGT	20	20	45.0	45.0	58.0	56.3	0.0	0.0	1.668164	25828	25947	229-229	1.000000	69.8
rpoC1-trnC-GCA_p2	rpoC1-trnC-GCA	2	AACTGCCTGACGTTGCATAT	ATTCAGGAAGAACAGGTGGT	20	20	45.0	45.0	57.9	56.3	8.5	0.0	1.768015	25828	25947	189-189	1.000000	69.7
rpoC1-trnC-GCA_p3	rpoC1-trnC-GCA	3	ACTGCCTGACGTTGCATATT	ATTCAGGAAGAACAGGTGGT	20	20	45.0	45.0	57.9	56.3	8.5	0.0	1.768015	25828	25947	188-188	1.000000	69.7
rpoC1-trnC-GCA_p4	rpoC1-trnC-GCA	4	CAACCCAGTTCCAACAATGC	ATTCAGGAAGAACAGGTGGT	20	20	50.0	45.0	57.8	56.3	0.0	0.0	1.807521	25828	25947	231-231	1.000000	68.7
rpoC1-trnC-GCA_p5	rpoC1-trnC-GCA	5	AACCCAGTTCCAACAATGCA	ATTCAGGAAGAACAGGTGGT	20	20	45.0	45.0	58.2	56.3	0.0	0.0	1.867217	25828	25947	230-230	1.000000	68.2
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	29601	30620	1112-1116	1.000000	72.3
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	29601	30620	1110-1114	1.000000	72.3
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	29601	30620	1108-1112	1.000000	71.8
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	29601	30620	1106-1110	1.000000	71.8
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.108380	29601	30620	1109-1113	1.000000	71.7
psbC_p1	psbC	1	AGGTATTCGTGCTTGGATGG	ACATTGGATCCCCTGTCTCA	20	20	50.0	50.0	57.7	58.0	0.0	9.9	0.360914	34881	36302	1480-1482	1.000000	78.2
psbC_p2	psbC	2	TATTCGTGCTTGGATGGCG	ACATTGGATCCCCTGTCTCA	19	20	52.6	50.0	58.3	58.0	0.0	9.9	1.339767	34881	36302	1477-1479	1.000000	70.4
psbC_p3	psbC	3	GGTATTCGTGCTTGGATGGC	ACATTGGATCCCCTGTCTCA	20	20	55.0	50.0	59.3	58.0	0.0	9.9	1.365697	34881	36302	1479-1481	1.000000	70.2
psbC_p4	psbC	4	AAGGTATTCGTGCTTGGATGG	ACATTGGATCCCCTGTCTCA	21	20	47.6	50.0	58.4	58.0	0.0	9.9	1.385300	34881	36302	1481-1483	1.000000	70.0
psbC_p5	psbC	5	GATGGCGGCTCAAGATCAG	ACATTGGATCCCCTGTCTCA	19	20	57.9	50.0	58.4	58.0	11.4	9.9	1.414270	34881	36302	1465-1467	1.000000	69.8
accD-psaI_p1	accD-psaI	1	TAAGCTCCACGCCTTTTTCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.5	58.0	0.0	26.9	0.496680	60217	60932	811-811	1.000000	76.4
accD-psaI_p2	accD-psaI	2	TAAGCTCCACGCCTTTTTCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.5	57.9	0.0	26.8	0.573214	60217	60932	817-817	1.000000	75.8
accD-psaI_p3	accD-psaI	3	GGTTCACAAGCGGCTGAATA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.6	58.0	1.8	26.9	0.578019	60217	60932	903-903	1.000000	75.7
accD-psaI_p4	accD-psaI	4	GGTTCACAAGCGGCTGAATA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.6	57.9	1.8	26.8	0.654553	60217	60932	909-909	1.000000	75.1
accD-psaI_p5	accD-psaI	5	TAAGCTCCACGCCTTTTTCC	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	58.5	57.5	0.0	8.9	0.921972	60217	60932	800-800	1.000000	73.0
ycf4-cemA_p1	ycf4-cemA	1	CGTTGACCCGTACTGATGAG	GAGAGAGAAATCCAACAGGACA	20	22	55.0	45.5	58.1	57.5	0.0	0.0	2.634214	62027	62896	1045-1045	1.000000	59.4
ycf4-cemA_p2	ycf4-cemA	2	CGTTGACCCGTACTGATGAG	TGAGAGAGAAATCCAACAGGAC	20	22	55.0	45.5	58.1	57.5	0.0	0.0	2.634214	62027	62896	1046-1046	1.000000	59.4
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCCGCTGAATTGG	GAGAGAGAAATCCAACAGGACA	20	22	45.0	45.5	57.8	57.5	14.2	0.0	2.696956	62027	62896	1002-1002	1.000000	58.9
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCCGCTGAATTGG	TGAGAGAGAAATCCAACAGGAC	20	22	45.0	45.5	57.8	57.5	14.2	0.0	2.696956	62027	62896	1003-1003	1.000000	58.9
ycf4-cemA_p5	ycf4-cemA	5	CGTTGACCCGTACTGATGAG	TCCAACAGGACAAAAACACT	20	20	55.0	40.0	58.1	55.3	0.0	0.0	2.785741	62027	62896	1035-1035	1.000000	58.2
cemA_p1	cemA	1	GTCGACGAATGAAGTGGGTT	ATGAAAATCCCCCGGGTAGA	20	20	50.0	50.0	58.2	58.1	0.0	21.8	0.306347	62897	63589	833-833	1.000000	77.6
cemA_p2	cemA	2	GAGTCGACGAATGAAGTGGG	ATGAAAATCCCCCGGGTAGA	20	20	55.0	50.0	58.4	58.1	10.5	21.8	0.468205	62897	63589	835-835	1.000000	76.4
cemA_p3	cemA	3	GTCGACGAATGAAGTGGGTT	GATGAAAATCCCCCGGGTAG	20	20	50.0	55.0	58.2	57.7	0.0	21.8	0.479938	62897	63589	834-834	1.000000	76.3
cemA_p4	cemA	4	GTCGACGAATGAAGTGGGTT	CCCCCGGGTAGAAAGTTTTT	20	20	50.0	50.0	58.2	57.7	0.0	21.8	0.505692	62897	63589	825-825	1.000000	76.1
cemA_p5	cemA	5	GTCGACGAATGAAGTGGGTT	ATCCCCCGGGTAGAAAGTTT	20	20	50.0	50.0	58.2	58.3	0.0	21.8	0.530504	62897	63589	827-827	1.000000	75.9
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	64761	65840	1137-1138	1.000000	75.0
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	64761	65840	1139-1140	1.000000	75.0
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	64761	65840	1136-1137	1.000000	74.5
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	64761	65840	1138-1139	1.000000	74.5
petA-psbJ_p5	petA-psbJ	5	CGCGTCCAGGGTCTTTTATT	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.755365	64761	65840	1215-1216	1.000000	74.3
psbB_p1	psbB	1	CCATTGAACAAGGGAGGTCC	GCACTCTGGTACTCTGATGTC	20	21	55.0	52.4	58.2	57.8	12.0	0.0	1.318508	74099	75625	1701-1703	1.000000	70.3
psbB_p2	psbB	2	CCATTGAACAAGGGAGGTCC	AGCGTTGATTCAAATGCACTC	20	21	55.0	42.9	58.2	57.8	12.0	20.6	1.368050	74099	75625	1716-1718	1.000000	69.8
psbB_p3	psbB	3	CCATTGAACAAGGGAGGTCC	GCGTTGATTCAAATGCACTCT	20	21	55.0	42.9	58.2	57.8	12.0	16.0	1.368050	74099	75625	1715-1717	1.000000	69.8
psbB_p4	psbB	4	CCATTGAACAAGGGAGGTCC	TGCACTCTGGTACTCTGATG	20	20	55.0	50.0	58.2	56.7	12.0	0.0	1.495560	74099	75625	1702-1704	1.000000	68.8
psbB_p5	psbB	5	CCATTGAACAAGGGAGGTCC	GCACTCTGGTACTCTGATGT	20	20	55.0	50.0	58.2	56.7	12.0	0.0	1.497833	74099	75625	1701-1703	1.000000	68.8
petB-petD_p1	petB-petD	1	TATCGTCCGACCGTTACTGA	CGACTGCAGTACCGATCAAA	20	20	50.0	50.0	58.0	58.0	0.0	21.7	0.031965	77924	78857	1866-1871	1.000000	80.4
petB-petD_p2	petB-petD	2	ACTATCGTCCGACCGTTACT	CGACTGCAGTACCGATCAAA	20	20	50.0	50.0	58.0	58.0	0.0	21.7	0.039039	77924	78857	1868-1873	1.000000	80.4
petB-petD_p3	petB-petD	3	GCCGCTTCTTACTGCTGTAT	CGACTGCAGTACCGATCAAA	20	20	50.0	50.0	58.1	58.0	4.1	21.7	0.067876	77924	78857	1465-1470	1.000000	80.3
petB-petD_p4	petB-petD	4	GTCTCGAGATTCAGGCGATC	CGACTGCAGTACCGATCAAA	20	20	55.0	50.0	58.0	58.0	19.9	21.7	0.054089	77924	78857	2006-2011	1.000000	80.2
petB-petD_p5	petB-petD	5	TATCGTCCGACCGTTACTGA	TGCAAATGGATCTGCTGGTT	20	20	50.0	45.0	58.0	58.1	0.0	6.3	0.095277	77924	78857	1658-1663	1.000000	80.0
infA-rps8_p1	infA-rps8	1	CCGTTGGGAAGTGATTCAGT	GAAAGAATCGGCGGAGAGTT	20	20	50.0	50.0	57.8	58.0	0.0	7.2	0.196544	81618	81725	203-203	1.000000	79.2
infA-rps8_p2	infA-rps8	2	CCGTTGGGAAGTGATTCAGT	CGGGAAGCTCGACTAGAAAG	20	20	50.0	55.0	57.8	57.8	0.0	0.0	0.381299	81618	81725	218-218	1.000000	77.7
infA-rps8_p3	infA-rps8	3	CCGTTGGGAAGTGATTCAGT	AAAGAATCGGCGGAGAGTTG	20	20	50.0	50.0	57.8	58.3	0.0	7.2	0.458151	81618	81725	202-202	1.000000	77.1
infA-rps8_p4	infA-rps8	4	ATGCCGTTGGGAAGTGATTC	GAAAGAATCGGCGGAGAGTT	20	20	50.0	50.0	58.5	58.0	0.0	7.2	0.547866	81618	81725	206-206	1.000000	76.4
infA-rps8_p5	infA-rps8	5	CCGTTGGGAAGTGATTCAGT	GCGGAGAGTTGTTGTGTTCT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.601951	81618	81725	193-193	1.000000	76.0
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGAGCA	GGTTCCCCTCCAAACCATTC	20	20	50.0	55.0	58.4	58.4	0.0	0.0	0.845159	83212	84259	1281-1281	1.000000	73.5
rpl16-rps3_p2	rpl16-rps3	2	GGGTTCAAGTGCCTGAAGAG	GGTTCCCCTCCAAACCATTC	20	20	55.0	55.0	58.5	58.4	0.0	0.0	0.914772	83212	84259	1283-1283	1.000000	72.9
rpl16-rps3_p3	rpl16-rps3	3	TTCAAGTGCCTGAAGAGCAT	GGTTCCCCTCCAAACCATTC	20	20	45.0	55.0	57.4	58.4	0.0	0.0	1.016907	83212	84259	1280-1280	1.000000	72.1
rpl16-rps3_p4	rpl16-rps3	4	GTTCAAGTGCCTGAAGAGCA	CCTCCAAACCATTCGAGCTA	20	20	50.0	50.0	58.4	57.3	0.0	0.0	1.093667	83212	84259	1275-1275	1.000000	71.5
rpl16-rps3_p5	rpl16-rps3	5	GGGTTCAAGTGCCTGAAGAG	CCTCCAAACCATTCGAGCTA	20	20	55.0	50.0	58.5	57.3	0.0	0.0	1.163280	83212	84259	1277-1277	1.000000	70.9
ccsA_p1	ccsA	1	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148453	115083	116045	1724-1725	1.000000	79.6
ccsA_p2	ccsA	2	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.154115	115083	116045	1723-1724	1.000000	79.6
ccsA_p3	ccsA	3	AAATTGGTAGACACGCTGCT	TCAGTAGCTTTTCGATGGCC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.209180	115083	116045	1731-1732	1.000000	79.2
ccsA_p4	ccsA	4	AAATTGGTAGACACGCTGCT	CAGTAGCTTTTCGATGGCCT	20	20	45.0	50.0	57.8	58.0	0.0	3.3	0.214842	115083	116045	1730-1731	1.000000	79.1
ccsA_p5	ccsA	5	TAGACACGCTGCTCTTAGGA	TTCTCGCCTGTTCACACAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.155536	115083	116045	1199-1199	1.000000	79.0
ccsA-ndhD_p1	ccsA-ndhD	1	TTCAGCAATTGTGGCGTCTA	ACCCGTACTAAGTATTGGTATGT	20	23	45.0	39.1	57.8	56.5	7.4	5.0	4.637882	116046	116288	451-452	1.000000	45.4
ccsA-ndhD_p2	ccsA-ndhD	2	GCAATTGTGGCGTCTATTGG	ACCCGTACTAAGTATTGGTATGT	20	23	50.0	39.1	57.8	56.5	7.4	5.0	4.653795	116046	116288	447-448	1.000000	45.3
ccsA-ndhD_p3	ccsA-ndhD	3	TCAGCAATTGTGGCGTCTAT	ACCCGTACTAAGTATTGGTATGT	20	23	45.0	39.1	57.6	56.5	7.4	5.0	4.862072	116046	116288	450-451	1.000000	43.6
ccsA-ndhD_p4	ccsA-ndhD	4	TTCAGCAATTGTGGCGTCTA	TCAGTTGAAAAGGTTGAAGT	20	20	45.0	35.0	57.8	52.8	7.4	0.0	5.339512	116046	116288	405-406	1.000000	42.8
ccsA-ndhD_p5	ccsA-ndhD	5	GCAATTGTGGCGTCTATTGG	TCAGTTGAAAAGGTTGAAGT	20	20	50.0	35.0	57.8	52.8	7.4	0.0	5.355425	116046	116288	401-402	1.000000	42.8
ndhD-psaC_p1	ndhD-psaC	1	AACCCGCAGATATTGGCAAA	GACCGAAGACTGTGTTGGTT	20	20	45.0	50.0	58.2	58.1	3.0	11.2	0.204528	117819	117910	289-289	1.000000	78.9
ndhD-psaC_p2	ndhD-psaC	2	ACCCGCAGATATTGGCAAAA	GACCGAAGACTGTGTTGGTT	20	20	45.0	50.0	58.2	58.1	3.0	11.2	0.204528	117819	117910	288-288	1.000000	78.9
ndhD-psaC_p3	ndhD-psaC	3	AACCCGCAGATATTGGCAAA	CCGACCGATTTCTTGAGTGT	20	20	45.0	50.0	58.2	57.9	3.0	0.0	0.234279	117819	117910	246-246	1.000000	78.8
ndhD-psaC_p4	ndhD-psaC	4	ACCCGCAGATATTGGCAAAA	CCGACCGATTTCTTGAGTGT	20	20	45.0	50.0	58.2	57.9	3.0	0.0	0.234279	117819	117910	245-245	1.000000	78.8
ndhD-psaC_p5	ndhD-psaC	5	CCCGCAGATATTGGCAAAAC	GACCGAAGACTGTGTTGGTT	20	20	50.0	50.0	57.8	58.1	3.0	11.2	0.258860	117819	117910	287-287	1.000000	78.5
ycf1_p1	ycf1	1	AGCCTGTCTCGTTAAATCGA	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	56.7	58.0	0.0	0.0	1.357546	129002	129121	258-258	1.000000	70.7
ycf1_p2	ycf1	2	AGCCTGTCTCGTTAAATCGAA	GCAGAATACCGTCACCCATT	21	20	42.9	50.0	57.4	58.0	0.0	0.0	1.626183	129002	129121	258-258	1.000000	68.5
ycf1_p3	ycf1	3	GCCTGTCTCGTTAAATCGAAG	GCAGAATACCGTCACCCATT	21	20	47.6	50.0	57.1	58.0	0.0	0.0	1.947406	129002	129121	257-257	1.000000	66.0
ycf1_p4	ycf1	4	GCCTGTCTCGTTAAATCGAA	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	55.9	58.0	0.0	0.0	2.139915	129002	129121	257-257	1.000000	64.4
ycf1_p5	ycf1	5	CCTGTCTCGTTAAATCGAAGGT	GCAGAATACCGTCACCCATT	22	20	45.5	50.0	58.2	58.0	0.0	0.0	2.192027	129002	129121	256-256	1.000000	64.0