primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbA-trnK-UUU_p1 psbA-trnK-UUU 1 TAGGCTTGTACTTTCGCGTC ACTCCATCCGACTAGTTCCG 20 20 50.0 55.0 58.0 58.6 5.8 0.0 0.622737 1159 1396 315-325 1.000000 80.5 psbA-trnK-UUU_p2 psbA-trnK-UUU 2 TAGGCTTGTACTTTCGCGTC ACTAGTTCCGGGTTCGAGTC 20 20 50.0 55.0 58.0 58.8 5.8 0.0 0.841127 1159 1396 305-315 1.000000 78.9 psbA-trnK-UUU_p3 psbA-trnK-UUU 3 TAGGCTTGTACTTTCGCGTC GACTAGTTCCGGGTTCGAGT 20 20 50.0 55.0 58.0 58.8 5.8 0.0 0.841127 1159 1396 306-316 1.000000 78.9 psbA-trnK-UUU_p4 psbA-trnK-UUU 4 TAGGCTTGTACTTTCGCGTC ATCCGACTAGTTCCGGGTTC 20 20 50.0 55.0 58.0 58.9 5.8 19.6 0.903693 1159 1396 310-320 1.000000 78.4 psbA-trnK-UUU_p5 psbA-trnK-UUU 5 GCTTGTACTTTCGCGTCTCT ACTCCATCCGACTAGTTCCG 20 20 50.0 55.0 58.3 58.6 5.8 0.0 0.910091 1159 1396 312-322 1.000000 78.3 psbI-trnS-GCU_p1 psbI-trnS-GCU 1 GTAATCCTGGGCGTGAAGAA GGGTTCGAATCCCTCTCTCT 20 20 50.0 55.0 57.6 58.2 0.0 17.2 0.613518 8238 8411 211-220 1.000000 82.8 psbI-trnS-GCU_p2 psbI-trnS-GCU 2 CTTATCTAACGATCCGGGGC GGGTTCGAATCCCTCTCTCT 20 20 55.0 55.0 57.6 58.2 0.0 17.2 0.582336 8238 8411 231-240 1.000000 82.7 psbI-trnS-GCU_p3 psbI-trnS-GCU 3 CTTATCTAACGATCCGGGGC TTCGAATCCCTCTCTCTCCG 20 20 55.0 55.0 57.6 58.3 0.0 0.0 0.683870 8238 8411 228-237 1.000000 82.0 psbI-trnS-GCU_p4 psbI-trnS-GCU 4 GTAATCCTGGGCGTGAAGAA TTCGAATCCCTCTCTCTCCG 20 20 50.0 55.0 57.6 58.3 0.0 0.0 0.715052 8238 8411 208-217 1.000000 82.0 psbI-trnS-GCU_p5 psbI-trnS-GCU 5 GTAATCCTGGGCGTGAAGAAT GGGTTCGAATCCCTCTCTCT 21 20 47.6 55.0 58.1 58.2 0.0 17.2 1.297701 8238 8411 211-220 1.000000 77.3 trnS-GCU-trnG-GCC_p1 trnS-GCU-trnG-GCC 1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.0 55.5 0.0 0.0 3.540425 8500 9820 925-1420 1.000000 62.2 trnS-GCU-trnG-GCC_p2 trnS-GCU-trnG-GCC 2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.0 55.5 0.0 0.0 3.541813 8500 9820 924-1419 1.000000 62.2 trnS-GCU-trnG-GCC_p3 trnS-GCU-trnG-GCC 3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.673141 8500 9820 928-1423 1.000000 61.2 trnS-GCU-trnG-GCC_p4 trnS-GCU-trnG-GCC 4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.673141 8500 9820 927-1422 1.000000 61.2 trnS-GCU-trnG-GCC_p5 trnS-GCU-trnG-GCC 5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.674529 8500 9820 926-1421 1.000000 61.2 trnG-GCC-trnR-UCU_p1 trnG-GCC-trnR-UCU 1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.081443 10582 10721 207-220 1.000000 70.4 trnG-GCC-trnR-UCU_p2 trnG-GCC-trnR-UCU 2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 58.1 56.9 0.0 0.0 2.110280 10582 10721 210-223 1.000000 70.1 trnG-GCC-trnR-UCU_p3 trnG-GCC-trnR-UCU 3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.164771 10582 10721 213-226 1.000000 69.6 trnG-GCC-trnR-UCU_p4 trnG-GCC-trnR-UCU 4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 20 22 55.0 45.5 58.1 57.9 0.0 0.0 2.193608 10582 10721 216-229 1.000000 69.4 trnG-GCC-trnR-UCU_p5 trnG-GCC-trnR-UCU 5 CCAACGTCGACTATAACCCC AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 57.8 56.9 14.7 0.0 2.265991 10582 10721 228-241 1.000000 68.6 trnR-UCU-atpA_p1 trnR-UCU-atpA 1 AGCCTTCCAAGCTAACGATG CGGCAGCTCAAATTAAAGCC 20 20 50.0 50.0 58.0 58.1 0.0 8.0 0.116522 10807 10962 826-859 1.000000 84.7 trnR-UCU-atpA_p2 trnR-UCU-atpA 2 AGCCTTCCAAGCTAACGATG CCCTGGTCGTGAAGCTTATC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.088513 10807 10962 1089-1122 1.000000 83.7 trnR-UCU-atpA_p3 trnR-UCU-atpA 3 AGCCTTCCAAGCTAACGATG CCTGGTCGTGAAGCTTATCC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.088513 10807 10962 1088-1121 1.000000 83.7 trnR-UCU-atpA_p4 trnR-UCU-atpA 4 AGCCTTCCAAGCTAACGATG GGTCGTGAAGCTTATCCAGG 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.088513 10807 10962 1085-1118 1.000000 83.7 trnR-UCU-atpA_p5 trnR-UCU-atpA 5 CCTAGCCTTCCAAGCTAACG GGTCGTGAAGCTTATCCAGG 20 20 55.0 55.0 58.1 58.1 0.0 0.0 0.117350 10807 10962 1088-1121 1.000000 83.5 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 GTGGACATTCCCTCATTTCCA CCGGGGATAAAGGATTTGCA 21 20 47.6 50.0 57.9 57.9 0.0 0.0 1.151851 27510 29318 1377-1892 1.000000 79.8 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 TGTGGACATTCCCTCATTTCC CCGGGGATAAAGGATTTGCA 21 20 47.6 50.0 57.9 57.9 0.0 0.0 1.151851 27510 29318 1378-1893 1.000000 79.8 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 GTGGACATTCCCTCATTTCCA ACCGGGGATAAAGGATTTGC 21 20 47.6 50.0 57.9 57.9 0.0 6.3 1.153317 27510 29318 1378-1893 1.000000 79.8 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 TGTGGACATTCCCTCATTTCC ACCGGGGATAAAGGATTTGC 21 20 47.6 50.0 57.9 57.9 0.0 6.3 1.153317 27510 29318 1379-1894 1.000000 79.8 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 GTGGACATTCCCTCATTTCCA TCGAACCGGGGATAAAGGAT 21 20 47.6 50.0 57.9 58.2 0.0 6.3 1.288632 27510 29318 1382-1897 1.000000 78.7 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 CAGCGCAGTAGCAATAAACG AGTTCAATCGGTCAGAGCAC 20 20 50.0 50.0 57.9 57.9 11.5 0.0 0.163209 31208 32346 1011-1263 1.000000 85.4 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 ATGAACAGCGCAGTAGCAAT AGTTCAATCGGTCAGAGCAC 20 20 45.0 50.0 58.3 57.9 11.5 0.0 0.343293 31208 32346 1016-1268 1.000000 83.9 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 TTGGCTGACTGTTTTGACGT AGTTCAATCGGTCAGAGCAC 20 20 45.0 50.0 58.3 57.9 0.0 0.0 0.340822 31208 32346 1068-1320 1.000000 83.8 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 TGAACAGCGCAGTAGCAATA AGTTCAATCGGTCAGAGCAC 20 20 45.0 50.0 57.6 57.9 11.5 0.0 0.464499 31208 32346 1015-1267 1.000000 83.0 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 AATGAACAGCGCAGTAGCAA AGTTCAATCGGTCAGAGCAC 20 20 45.0 50.0 58.5 57.9 11.5 0.0 0.563386 31208 32346 1017-1269 1.000000 82.2 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.175803 32959 33840 888-986 1.000000 86.8 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 20 20 50.0 55.0 57.8 58.1 0.0 0.0 0.233900 32959 33840 867-965 1.000000 86.4 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.256054 32959 33840 864-962 1.000000 86.2 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.287509 32959 33840 866-964 1.000000 86.0 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.320736 32959 33840 885-983 1.000000 85.6 trnF-GAA-trnV-UAC_p1 trnF-GAA-trnV-UAC 1 CCTCGTGTCACCAGTTCAAA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.249179 50121 50697 633-2382 1.000000 83.1 trnF-GAA-trnV-UAC_p2 trnF-GAA-trnV-UAC 2 CCTCGTGTCACCAGTTCAAA GAAGGTCTACGGTTCGAGTC 20 20 50.0 55.0 58.1 57.5 0.0 0.0 0.592951 50121 50697 632-2381 1.000000 80.4 trnF-GAA-trnV-UAC_p3 trnF-GAA-trnV-UAC 3 AATCCTCGTGTCACCAGTTC AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.654649 50121 50697 636-2385 1.000000 79.9 trnF-GAA-trnV-UAC_p4 trnF-GAA-trnV-UAC 4 TCGGGATAGCTCAGTTGGTA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.6 57.8 0.0 0.0 0.644691 50121 50697 671-2420 1.000000 79.8 trnF-GAA-trnV-UAC_p5 trnF-GAA-trnV-UAC 5 CCTCGTGTCACCAGTTCAAA GTTCGAGTCCGTATAGCCCT 20 20 50.0 55.0 58.1 58.7 0.0 0.0 0.735245 50121 50697 621-2370 1.000000 79.3 rbcL-accD_p1 rbcL-accD 1 TTTCGACCCAGTGGATAAGC AGACAACATTGAATTGAACCA 20 21 50.0 33.3 57.6 53.9 12.0 0.0 5.496376 56098 56904 872-882 1.000000 46.9 rbcL-accD_p2 rbcL-accD 2 TTTCGACCCAGTGGATAAGC AGACAACATTGAATTGAACCAA 20 22 50.0 31.8 57.6 54.7 12.0 0.0 5.659736 56098 56904 872-882 1.000000 46.9 rbcL-accD_p3 rbcL-accD 3 TTTCGACCCAGTGGATAAGC AGACAACATTGAATTGAACCAAT 20 23 50.0 30.4 57.6 55.3 12.0 0.0 6.102146 56098 56904 872-882 1.000000 46.9 rbcL-accD_p4 rbcL-accD 4 TTTCGACCCAGTGGATAAGC GACAACATTGAATTGAACCA 20 20 50.0 35.0 57.6 52.2 12.0 0.0 6.148849 56098 56904 871-881 1.000000 46.9 rbcL-accD_p5 rbcL-accD 5 ATTTCGACCCAGTGGATAAGC AGACAACATTGAATTGAACCA 21 21 47.6 33.3 58.1 53.9 12.0 0.0 6.180559 56098 56904 873-883 1.000000 46.9 psbE-petL_p1 psbE-petL 1 CAACCCGCAATGAATAGGGA GCTGGGGTTATAGTTGAAGCA 20 21 50.0 47.6 57.9 58.0 6.4 0.0 1.052003 64564 65701 1130-1308 1.000000 78.8 psbE-petL_p2 psbE-petL 2 CAACCCGCAATGAATAGGGA GGGTTATAGTTGAAGCAGCCA 20 21 50.0 47.6 57.9 58.0 6.4 0.0 1.052003 64564 65701 1126-1304 1.000000 78.8 psbE-petL_p3 psbE-petL 3 CAACCCGCAATGAATAGGGA TGGGGTTATAGTTGAAGCAGC 20 21 50.0 47.6 57.9 58.0 6.4 0.0 1.052003 64564 65701 1128-1306 1.000000 78.8 psbE-petL_p4 psbE-petL 4 ACTGACGAATAACCAACCCG GGGTTATAGTTGAAGCAGCCA 20 21 50.0 47.6 57.9 58.0 0.0 0.0 1.089618 64564 65701 1139-1317 1.000000 78.5 psbE-petL_p5 psbE-petL 5 ACTGACGAATAACCAACCCG TGGGGTTATAGTTGAAGCAGC 20 21 50.0 47.6 57.9 58.0 0.0 0.0 1.089618 64564 65701 1141-1319 1.000000 78.5 clpP-psbB_p1 clpP-psbB 1 CGCACTATACGTCAACCCAA CAACCAGAAACCAGAGCTGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.028080 72098 72793 870-911 1.000000 89.0 clpP-psbB_p2 clpP-psbB 2 CGCACTATACGTCAACCCAA AAGCAAGCGATTCCTGAGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.022307 72098 72793 1224-1265 1.000000 86.7 clpP-psbB_p3 clpP-psbB 3 CGCACTATACGTCAACCCAA ACACCCATATTCCAGGACCA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.021142 72098 72793 1277-1318 1.000000 86.4 clpP-psbB_p4 clpP-psbB 4 CGCACTATACGTCAACCCAA TAGACCCGCTAATATGCCCA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.019307 72098 72793 1414-1455 1.000000 85.8 clpP-psbB_p5 clpP-psbB 5 CGCACTATACGTCAACCCAA TAGCTGCCAAGAAGCACAAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.021097 72098 72793 1106-1118 0.500000 62.4 psbB-psbT_p1 psbB-psbT 1 GCTCAAGTGGAATTTGGAGC TGGAACTTTAGGTGGTTCTCG 20 21 50.0 47.6 57.4 57.6 0.0 0.0 2.040194 74321 74711 537-553 1.000000 71.1 psbB-psbT_p2 psbB-psbT 2 GCTCAAGTGGAATTTGGAGC GGAACTTTAGGTGGTTCTCGA 20 21 50.0 47.6 57.4 57.3 0.0 0.0 2.316700 74321 74711 536-552 1.000000 68.8 psbB-psbT_p3 psbB-psbT 3 TCCAACTACAAGGAGACAGGT TGGAACTTTAGGTGGTTCTCG 21 21 47.6 47.6 58.0 57.6 0.0 0.0 2.423912 74321 74711 499-515 1.000000 68.2 psbB-psbT_p4 psbB-psbT 4 GCTCAAGTGGAATTTGGAGC GGAACTTTAGGTGGTTCTCGAA 20 22 50.0 45.5 57.4 58.0 0.0 0.0 2.644887 74321 74711 536-552 1.000000 66.2 psbB-psbT_p5 psbB-psbT 5 TCCAACTACAAGGAGACAGGT GGAACTTTAGGTGGTTCTCGA 21 21 47.6 47.6 58.0 57.3 0.0 0.0 2.700419 74321 74711 498-514 1.000000 66.0 psbT-psbN_p1 psbT-psbN 1 CGAGAACCACCTAAAGTTCCA CCTTCGAAGAACATGGGGAC 21 20 47.6 55.0 57.6 58.3 0.0 26.6 1.657194 74820 74881 124-124 1.000000 70.6 psbT-psbN_p2 psbT-psbN 2 TCGAGAACCACCTAAAGTTCC CCTTCGAAGAACATGGGGAC 21 20 47.6 55.0 57.3 58.3 0.0 26.6 1.933700 74820 74881 125-125 1.000000 68.4 psbT-psbN_p3 psbT-psbN 3 CGAGAACCACCTAAAGTTCCA ATCCCTTCGAAGAACATGGG 21 20 47.6 50.0 57.6 57.3 0.0 26.6 2.113899 74820 74881 127-127 1.000000 66.9 psbT-psbN_p4 psbT-psbN 4 CGAGAACCACCTAAAGTTCCA CTTCGAAGAACATGGGGACT 21 20 47.6 50.0 57.6 57.2 0.0 20.7 2.166424 74820 74881 123-123 1.000000 66.6 psbT-psbN_p5 psbT-psbN 5 TTCGAGAACCACCTAAAGTTCC CCTTCGAAGAACATGGGGAC 22 20 45.5 55.0 58.0 58.3 0.0 26.6 2.261887 74820 74881 126-126 1.000000 65.7 petD-rpoA_p1 petD-rpoA 1 GTACCGCAGTAGCTCTTTGG TTGAGCACTGTACAGAAGCA 20 20 55.0 45.0 58.4 57.1 0.0 31.3 1.267111 78466 78637 312-323 1.000000 73.4 petD-rpoA_p2 petD-rpoA 2 GGTACCGCAGTAGCTCTTTG TTGAGCACTGTACAGAAGCA 20 20 55.0 45.0 58.4 57.1 0.0 31.3 1.267111 78466 78637 313-324 1.000000 73.3 petD-rpoA_p3 petD-rpoA 3 CCGCAGTAGCTCTTTGGTTA TTGAGCACTGTACAGAAGCA 20 20 50.0 45.0 57.6 57.1 0.0 31.3 1.288772 78466 78637 309-320 1.000000 73.2 petD-rpoA_p4 petD-rpoA 4 TGGTTAGGGATTGGAGCAAC TTGAGCACTGTACAGAAGCA 20 20 50.0 45.0 57.5 57.1 0.0 31.3 1.416095 78466 78637 295-306 1.000000 72.4 petD-rpoA_p5 petD-rpoA 5 GTACCGCAGTAGCTCTTTGG TGAGCACTGTACAGAAGCAT 20 20 55.0 45.0 58.4 56.9 0.0 31.3 1.495408 78466 78637 311-322 1.000000 71.5 trnN-GUU-rpl32_p1 trnN-GUU-rpl32 1 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTACGTTTTT 20 22 50.0 27.3 58.1 52.0 8.6 0.0 8.083655 109190 110005 699-1033 1.000000 52.0 trnN-GUU-rpl32_p2 trnN-GUU-rpl32 2 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTACGTTTT 20 22 50.0 27.3 58.1 52.0 8.6 0.0 8.083655 109190 110005 700-1034 1.000000 52.0 trnN-GUU-rpl32_p3 trnN-GUU-rpl32 3 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTACGTTT 20 22 50.0 27.3 58.1 52.0 8.6 0.0 8.083655 109190 110005 701-1035 1.000000 52.0 trnN-GUU-rpl32_p4 trnN-GUU-rpl32 4 ACGACCAGTCAGTTAACAGC TTTGACATAGAAGTACGTTTTT 20 22 50.0 27.3 57.8 52.0 8.6 0.0 8.113005 109190 110005 705-1039 1.000000 52.0 trnN-GUU-rpl32_p5 trnN-GUU-rpl32 5 ACGACCAGTCAGTTAACAGC TTTTGACATAGAAGTACGTTTT 20 22 50.0 27.3 57.8 52.0 8.6 0.0 8.113005 109190 110005 706-1040 1.000000 52.0 psaC-rps15_p1 psaC-rps15 1 GGACAAGCGGATTCACATCT ATCACAAAGGGGTCTACGAA 20 20 50.0 45.0 58.0 56.2 0.0 0.0 1.862502 113560 115409 1768-2190 1.000000 73.1 psaC-rps15_p2 psaC-rps15 2 GGACAAGCGGATTCACATCT TCACAAAGGGGTCTACGAAT 20 20 50.0 45.0 58.0 56.2 0.0 0.0 1.862502 113560 115409 1767-2189 1.000000 73.1 psaC-rps15_p3 psaC-rps15 3 GTCCATCGGTTTCCAGTCTC ATCACAAAGGGGTCTACGAA 20 20 55.0 45.0 58.0 56.2 0.0 0.0 1.844204 113560 115409 2229-2661 1.000000 72.9 psaC-rps15_p4 psaC-rps15 4 GTCCATCGGTTTCCAGTCTC TCACAAAGGGGTCTACGAAT 20 20 55.0 45.0 58.0 56.2 0.0 0.0 1.844204 113560 115409 2228-2660 1.000000 72.9 psaC-rps15_p5 psaC-rps15 5 TATACCAGAAAAGCCCGTGC TCACAAAGGGGTCTACGAAT 20 20 50.0 45.0 58.0 56.2 0.0 0.0 1.865345 113560 115409 1950-2372 1.000000 72.9