primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(UCC)_p1	trnS(GCU)-trnG(UCC)	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8565	9311	793-850	1.000000	56.6
trnS(GCU)-trnG(UCC)_p2	trnS(GCU)-trnG(UCC)	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8565	9311	792-849	1.000000	56.5
trnS(GCU)-trnG(UCC)_p3	trnS(GCU)-trnG(UCC)	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8565	9311	796-853	1.000000	55.5
trnS(GCU)-trnG(UCC)_p4	trnS(GCU)-trnG(UCC)	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8565	9311	795-852	1.000000	55.5
trnS(GCU)-trnG(UCC)_p5	trnS(GCU)-trnG(UCC)	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8565	9311	794-851	1.000000	55.5
atpF-atpH_p1	atpF-atpH	1	TGGCCCAAAGAAACGAAAGA	CCGAGGCAGAGGGAAAAATA	20	20	45.0	50.0	57.9	57.3	0.0	0.0	0.770146	13265	13635	526-532	1.000000	76.4
atpF-atpH_p2	atpF-atpH	2	GTGGCCCAAAGAAACGAAAG	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.844297	13265	13635	527-533	1.000000	75.8
atpF-atpH_p3	atpF-atpH	3	AGTGGCCCAAAGAAACGAAA	CCGAGGCAGAGGGAAAAATA	20	20	45.0	50.0	58.2	57.3	0.0	0.0	0.939802	13265	13635	528-534	1.000000	75.0
atpF-atpH_p4	atpF-atpH	4	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	58.3	57.3	0.0	0.0	0.968568	13265	13635	556-562	1.000000	74.7
atpF-atpH_p5	atpF-atpH	5	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGA	20	21	45.0	47.6	57.9	57.7	0.0	0.0	1.322031	13265	13635	521-527	1.000000	72.0
rpoB-trnC(GCA)_p1	rpoB-trnC(GCA)	1	ATCATTTGCATCCCCGAGC	ATGTTGATTAGGCGACACCC	19	20	52.6	50.0	58.3	58.0	0.0	0.0	1.313867	27183	28309	1217-1277	1.000000	74.5
rpoB-trnC(GCA)_p2	rpoB-trnC(GCA)	2	ATCATTTGCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	52.6	50.0	58.3	58.1	0.0	0.0	1.357608	27183	28309	1199-1259	1.000000	74.2
rpoB-trnC(GCA)_p3	rpoB-trnC(GCA)	3	CATTTGCATCCCCGAGCAT	ATGTTGATTAGGCGACACCC	19	20	52.6	50.0	58.6	58.0	0.0	0.0	1.616084	27183	28309	1215-1275	1.000000	72.1
rpoB-trnC(GCA)_p4	rpoB-trnC(GCA)	4	ATCATTTGCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	19	20	52.6	50.0	58.3	58.4	0.0	14.6	1.630971	27183	28309	1181-1241	1.000000	72.1
rpoB-trnC(GCA)_p5	rpoB-trnC(GCA)	5	CATTTGCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	19	20	52.6	50.0	58.6	58.1	0.0	0.0	1.659825	27183	28309	1197-1257	1.000000	71.8
psbZ-trnG(GCC)_p1	psbZ-trnG(GCC)	1	ACCCGTCGTATTTGCTTCTC	AATCCTGTCTCCGCAACATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	36526	36816	634-704	1.000000	87.0
psbZ-trnG(GCC)_p2	psbZ-trnG(GCC)	2	ACCCGTCGTATTTGCTTCTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031559	36526	36816	688-758	1.000000	86.8
psbZ-trnG(GCC)_p3	psbZ-trnG(GCC)	3	ACCCGTCGTATTTGCTTCTC	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.019256	36526	36816	961-1031	1.000000	86.2
psbZ-trnG(GCC)_p4	psbZ-trnG(GCC)	4	TCCATTTCCCCTGACTCCAT	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.027664	36526	36816	1200-1270	1.000000	85.0
psbZ-trnG(GCC)_p5	psbZ-trnG(GCC)	5	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	20	20	55.0	50.0	58.0	58.0	0.0	9.5	0.034641	36526	36816	1384-1454	1.000000	84.4
psaA-ycf3_p1	psaA-ycf3	1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136672	42191	43142	948-1160	1.000000	89.2
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	42191	43142	929-1141	1.000000	89.1
psaA-ycf3_p3	psaA-ycf3	3	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.259531	42191	43142	877-1089	1.000000	88.6
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	42191	43142	858-1070	1.000000	88.6
psaA-ycf3_p5	psaA-ycf3	5	TGAGAAATGACCCGGTTTGG	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.287302	42191	43142	881-1093	1.000000	88.4
trnT(UGU)-trnL(UAA)_p1	trnT(UGU)-trnL(UAA)	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47410	47990	625-824	1.000000	82.7
trnT(UGU)-trnL(UAA)_p2	trnT(UGU)-trnL(UAA)	2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	47410	47990	676-875	1.000000	81.8
trnT(UGU)-trnL(UAA)_p3	trnT(UGU)-trnL(UAA)	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47410	47990	657-856	1.000000	81.7
trnT(UGU)-trnL(UAA)_p4	trnT(UGU)-trnL(UAA)	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47410	47990	619-818	1.000000	81.4
trnT(UGU)-trnL(UAA)_p5	trnT(UGU)-trnL(UAA)	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47410	47990	657-856	1.000000	80.5
atpB-rbcL_p1	atpB-rbcL	1	CCCCAGAACCGGAAGTAGTA	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	9.5	0.0	0.444865	55480	56245	851-851	1.000000	77.1
atpB-rbcL_p2	atpB-rbcL	2	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	55480	56245	927-927	1.000000	76.9
atpB-rbcL_p3	atpB-rbcL	3	CGGACCGATAATTTGGACGA	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	8.6	0.0	0.514363	55480	56245	904-904	1.000000	76.5
atpB-rbcL_p4	atpB-rbcL	4	TAATTTGGACGATACGCCCC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.545096	55480	56245	896-896	1.000000	76.3
atpB-rbcL_p5	atpB-rbcL	5	ACCGGACCGATAATTTGGAC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.7	58.3	17.5	0.0	0.622239	55480	56245	906-906	1.000000	75.6
cemA-petA_p1	cemA-petA	1	CGTGTATCTCCGTCACTTGT	GCACTTGAAATGGATGTCCG	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.181051	63186	63426	380-404	1.000000	76.7
cemA-petA_p2	cemA-petA	2	TCGTGTATCTCCGTCACTTG	GCACTTGAAATGGATGTCCG	20	20	50.0	50.0	57.1	57.5	0.0	0.0	1.463410	63186	63426	381-405	1.000000	74.4
cemA-petA_p3	cemA-petA	3	ATCGTGTATCTCCGTCACTTG	GCACTTGAAATGGATGTCCG	21	20	47.6	50.0	57.6	57.5	0.0	0.0	1.972050	63186	63426	382-406	1.000000	70.4
cemA-petA_p4	cemA-petA	4	CGTGTATCTCCGTCACTTGT	ATCAGGAGCGATACGGAAAT	20	20	50.0	45.0	57.4	56.5	0.0	0.0	2.132023	63186	63426	347-371	1.000000	69.7
cemA-petA_p5	cemA-petA	5	CGTGTATCTCCGTCACTTGT	CGCAAAAATGGGATATGCACT	20	21	50.0	42.9	57.4	57.6	0.0	0.0	2.072914	63186	63426	396-420	1.000000	69.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	55.9	6.4	3.6	2.152316	66231	66940	855-1073	1.000000	69.9
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	55.9	24.9	3.6	2.257324	66231	66940	778-996	1.000000	69.7
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	55.9	0.0	3.6	2.189931	66231	66940	868-1086	1.000000	69.5
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AGCCACTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	55.9	0.0	3.6	2.272002	66231	66940	864-1082	1.000000	68.8
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	55.9	0.0	3.6	2.288332	66231	66940	863-1081	1.000000	68.7
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	58.0	57.9	4.9	12.9	0.100097	69972	70776	870-902	1.000000	79.9
rpl20-rps12_p2	rpl20-rps12	2	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	20	20	55.0	50.0	58.2	57.9	22.3	12.9	0.302025	69972	70776	931-963	1.000000	78.3
rpl20-rps12_p3	rpl20-rps12	3	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	58.3	57.9	22.3	12.9	0.384764	69972	70776	930-962	1.000000	77.6
rpl20-rps12_p4	rpl20-rps12	4	TTTGTTCTACGTCTCCGAGC	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	57.7	57.9	0.0	12.9	0.408902	69972	70776	883-915	1.000000	77.4
rpl20-rps12_p5	rpl20-rps12	5	TGTTCTACGTCTCCGAGCTA	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	57.6	57.9	0.0	12.9	0.457608	69972	70776	881-913	1.000000	77.1
rps12-trnV(GAC)_p1	rps12-trnV(GAC)	1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	99086	100700	1711-1757	1.000000	78.9
rps12-trnV(GAC)_p2	rps12-trnV(GAC)	2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	99086	100700	1715-1761	1.000000	78.8
rps12-trnV(GAC)_p3	rps12-trnV(GAC)	3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	99086	100700	1710-1756	1.000000	78.8
rps12-trnV(GAC)_p4	rps12-trnV(GAC)	4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	99086	100700	1713-1759	1.000000	77.9
rps12-trnV(GAC)_p5	rps12-trnV(GAC)	5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	20	20	45.0	55.0	57.7	57.7	16.5	5.4	0.606988	99086	100700	1696-1742	1.000000	77.4
rpl32-trnL(UAG)_p1	rpl32-trnL(UAG)	1	GGGATATTGGGCAGCGTTAA	TACCATTTCACCATAGCGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062547	113618	114334	845-858	1.000000	81.8
rpl32-trnL(UAG)_p2	rpl32-trnL(UAG)	2	GGGATATTGGGCAGCGTTAA	TAGGAGATGATATGCCGCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087149	113618	114334	916-929	1.000000	81.5
rpl32-trnL(UAG)_p3	rpl32-trnL(UAG)	3	GGGCAGCGTTAAAAGCATTT	TACCATTTCACCATAGCGGC	20	20	45.0	50.0	57.9	58.0	6.9	0.0	0.117055	113618	114334	837-850	1.000000	81.4
rpl32-trnL(UAG)_p4	rpl32-trnL(UAG)	4	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCATTTCACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119103	113618	114334	853-866	1.000000	81.4
rpl32-trnL(UAG)_p5	rpl32-trnL(UAG)	5	GGGCAGCGTTAAAAGCATTT	TAGGAGATGATATGCCGCCA	20	20	45.0	50.0	57.9	58.1	6.9	0.0	0.141657	113618	114334	908-921	1.000000	81.1
ndhD-psaC_p1	ndhD-psaC	1	GCAAGGAAAATGGTTCGTGG	CCCACGGATTTCTTGAGTGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.249577	117236	117334	207-216	1.000000	84.8
ndhD-psaC_p2	ndhD-psaC	2	GCAAGGAAAATGGTTCGTGG	AGGACTGCGTTGGTTGTAAG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.185620	117236	117334	245-254	1.000000	84.3
ndhD-psaC_p3	ndhD-psaC	3	GCAAGGAAAATGGTTCGTGG	GGACTGCGTTGGTTGTAAGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.190876	117236	117334	244-253	1.000000	84.3
ndhD-psaC_p4	ndhD-psaC	4	GCAAGGAAAATGGTTCGTGG	GAGGACTGCGTTGGTTGTAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.190876	117236	117334	246-255	1.000000	84.2
ndhD-psaC_p5	ndhD-psaC	5	GCAAGGAAAATGGTTCGTGG	AAGAGATGTGAATCCGCCTG	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.386929	117236	117334	228-237	1.000000	83.1
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAATTG	20	22	50.0	45.5	58.0	57.7	0.0	19.9	2.314736	118873	119233	476-496	1.000000	69.8
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAAT	20	20	50.0	45.0	58.0	55.6	0.0	19.9	2.489985	118873	119233	476-496	1.000000	68.4
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAATGAAAAGGCCGAAT	CGGTTACCGGTGTCAATAATTG	20	22	45.0	45.5	57.8	57.7	0.0	19.9	2.497487	118873	119233	539-559	1.000000	67.6
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAATT	20	21	50.0	42.9	58.0	56.3	0.0	19.9	2.709201	118873	119233	476-496	1.000000	66.6
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAATGAAAAGGCCGAAT	CGGTTACCGGTGTCAATAAT	20	20	45.0	45.0	57.8	55.6	0.0	19.9	2.672736	118873	119233	539-559	1.000000	66.2
ndhA_p1	ndhA	1	TGAGACAAGCTGTGAAAATAGACT	GCCGGGTTTTATCAACTTGC	24	20	37.5	50.0	58.1	58.0	0.0	2.2	4.150820	119815	121878	2168-2211	1.000000	49.5
ndhA_p2	ndhA	2	TGAGACAAGCTGTGAAAATAGAC	GCCGGGTTTTATCAACTTGC	23	20	39.1	50.0	56.8	58.0	0.0	2.2	4.190932	119815	121878	2168-2211	1.000000	49.2
ndhA_p3	ndhA	3	TGAGACAAGCTGTGAAAATAGACT	CGCCGGGTTTTATCAACTTG	24	20	37.5	50.0	58.1	57.7	0.0	2.2	4.398064	119815	121878	2169-2212	1.000000	47.6
ndhA_p4	ndhA	4	TGAGACAAGCTGTGAAAATAGAC	CGCCGGGTTTTATCAACTTG	23	20	39.1	50.0	56.8	57.7	0.0	2.2	4.438176	119815	121878	2169-2212	1.000000	47.2
ndhA_p5	ndhA	5	TTTGAGACAAGCTGTGAAAA	GCCGGGTTTTATCAACTTGC	20	20	35.0	50.0	53.6	58.0	0.0	2.2	4.447854	119815	121878	2170-2213	1.000000	47.2
rrn23-trnA(UGC)_p1	rrn23-trnA(UGC)	1	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	20	20	55.0	55.0	57.9	57.9	11.3	0.0	0.212163	134108	134274	224-240	1.000000	84.2
rrn23-trnA(UGC)_p2	rrn23-trnA(UGC)	2	TCGATTACTACGCCCTTCCT	GGTTCGAGTCCGCTTATCTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.185533	134108	134274	246-262	1.000000	83.9
rrn23-trnA(UGC)_p3	rrn23-trnA(UGC)	3	TCGATTACTACGCCCTTCCT	GTTCGAGTCCGCTTATCTCC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.185533	134108	134274	245-261	1.000000	83.9
rrn23-trnA(UGC)_p4	rrn23-trnA(UGC)	4	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	134108	134274	297-313	1.000000	83.4
rrn23-trnA(UGC)_p5	rrn23-trnA(UGC)	5	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	134108	134274	296-312	1.000000	83.4
trnV(GAC)-rps12_p1	trnV(GAC)-rps12	1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	138330	139944	1711-1757	1.000000	77.3
trnV(GAC)-rps12_p2	trnV(GAC)-rps12	2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	138330	139944	1715-1761	1.000000	77.3
trnV(GAC)-rps12_p3	trnV(GAC)-rps12	3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	138330	139944	1710-1756	1.000000	77.3
trnV(GAC)-rps12_p4	trnV(GAC)-rps12	4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	138330	139944	1713-1759	1.000000	76.4
trnV(GAC)-rps12_p5	trnV(GAC)-rps12	5	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	138330	139944	1696-1742	1.000000	75.8