primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.532399	175	898	811-868	1.000000	78.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.533769	175	898	812-869	1.000000	78.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.548744	175	898	871-928	1.000000	78.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.6	58.0	6.3	0.0	0.593672	175	898	814-871	1.000000	78.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.6	58.0	6.3	0.0	0.593672	175	898	815-872	1.000000	78.1
ycf1_p1	ycf1	1	TGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTCT	20	22	45.0	40.9	56.4	57.5	0.0	0.0	4.091765	11554	11673	247-289	1.000000	55.4
ycf1_p2	ycf1	2	TGGTAATGTCTGATCTCGCA	AGAATCTTGGTTCTTCCCAGA	20	21	45.0	42.9	56.4	56.3	0.0	0.0	4.381659	11554	11673	254-296	1.000000	53.0
ycf1_p3	ycf1	3	ATGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTCT	21	22	42.9	40.9	56.9	57.5	0.0	0.0	4.557287	11554	11673	248-290	1.000000	51.7
ycf1_p4	ycf1	4	TGGTAATGTCTGATCTCGCAT	TGGTTCTTCCCAGAATTTGTCT	21	22	42.9	40.9	56.9	57.5	0.0	0.0	4.557287	11554	11673	247-289	1.000000	51.7
ycf1_p5	ycf1	5	TGGTAATGTCTGATCTCGCA	TGGTTCTTCCCAGAATTTGTC	20	21	45.0	42.9	56.4	56.1	0.0	0.0	4.576425	11554	11673	247-289	1.000000	51.6
trnI-GAU_p1	trnI-GAU	1	TCTATTCTTTTCCCTGGCGC	GGGGTTTTTCTCGCTTTTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.187302	22006	23020	1092-1093	1.000000	78.5
trnI-GAU_p2	trnI-GAU	2	GCCAAGTGGAGTATGCATGA	GGGGTTTTTCTCGCTTTTGG	20	20	50.0	50.0	58.0	57.9	10.2	0.0	0.191602	22006	23020	1129-1130	1.000000	78.5
trnI-GAU_p3	trnI-GAU	3	AGCCAAGTGGAGTATGCATG	GGGGTTTTTCTCGCTTTTGG	20	20	50.0	50.0	58.0	57.9	10.2	0.0	0.197360	22006	23020	1130-1131	1.000000	78.4
trnI-GAU_p4	trnI-GAU	4	TCTATTCTTTTCCCTGGCGC	TTCTCGCTTTTGGCATAGCA	20	20	50.0	45.0	58.0	58.2	0.0	18.4	0.218458	22006	23020	1085-1086	1.000000	78.3
trnI-GAU_p5	trnI-GAU	5	GCCAAGTGGAGTATGCATGA	TTCTCGCTTTTGGCATAGCA	20	20	50.0	45.0	58.0	58.2	10.2	18.4	0.222758	22006	23020	1122-1123	1.000000	78.2
trnI-GAU-rrn16S_p1	trnI-GAU-rrn16S	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	23021	23336	410-410	1.000000	78.9
trnI-GAU-rrn16S_p2	trnI-GAU-rrn16S	2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	57.8	57.7	0.0	7.8	0.462074	23021	23336	442-442	1.000000	78.8
trnI-GAU-rrn16S_p3	trnI-GAU-rrn16S	3	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	23021	23336	461-461	1.000000	78.7
trnI-GAU-rrn16S_p4	trnI-GAU-rrn16S	4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.473195	23021	23336	464-464	1.000000	78.6
trnI-GAU-rrn16S_p5	trnI-GAU-rrn16S	5	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	57.8	58.3	4.7	0.0	0.476037	23021	23336	483-483	1.000000	78.5
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	25131	26843	1870-1870	1.000000	77.1
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	25131	26843	1809-1809	1.000000	76.8
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	25131	26843	1813-1813	1.000000	76.7
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	25131	26843	1808-1808	1.000000	76.7
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	25131	26843	1811-1811	1.000000	75.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.352001	49421	50487	1017-1274	1.000000	82.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.373069	49421	50487	962-1219	1.000000	82.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.519280	49421	50487	1018-1275	1.000000	81.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.540349	49421	50487	963-1220	1.000000	81.3
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	20	20	55.0	50.0	58.0	57.4	0.0	0.0	0.572870	49421	50487	1016-1273	1.000000	81.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	55242	56225	1009-1041	1.000000	83.5
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	57.9	57.8	0.0	21.3	0.324323	55242	56225	1025-1057	1.000000	82.6
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.2	57.8	1.4	21.3	0.359803	55242	56225	1010-1042	1.000000	82.3
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.390265	55242	56225	1024-1056	1.000000	82.0
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	58.3	57.8	0.0	21.3	0.438039	55242	56225	1019-1051	1.000000	81.7
atpI-rps2_p1	atpI-rps2	1	GTTGGCCTACTTCTACACCC	ATTTCGATTCCAGCCAACGA	20	20	55.0	45.0	57.6	57.9	10.3	0.0	0.506202	56970	57224	436-437	1.000000	76.7
atpI-rps2_p2	atpI-rps2	2	GTTGGCCTACTTCTACACCC	TTTCGATTCCAGCCAACGAT	20	20	55.0	45.0	57.6	57.9	10.3	0.0	0.506202	56970	57224	435-436	1.000000	76.7
atpI-rps2_p3	atpI-rps2	3	GTTGGCCTACTTCTACACCC	CAGCCAACGATGACGCTATA	20	20	55.0	50.0	57.6	57.9	10.3	0.0	0.532453	56970	57224	426-427	1.000000	76.5
atpI-rps2_p4	atpI-rps2	4	GGCCTACTTCTACACCCGAT	ATTTCGATTCCAGCCAACGA	20	20	55.0	45.0	58.6	57.9	0.0	0.0	0.696909	56970	57224	433-434	1.000000	75.2
atpI-rps2_p5	atpI-rps2	5	GGCCTACTTCTACACCCGAT	TTTCGATTCCAGCCAACGAT	20	20	55.0	45.0	58.6	57.9	0.0	0.0	0.696909	56970	57224	432-433	1.000000	75.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	58.4	58.1	0.0	14.6	0.480541	68489	69823	1402-1429	1.000000	77.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	57.6	58.1	0.0	14.6	0.506504	68489	69823	1403-1430	1.000000	77.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.885652	68489	69823	1428-1455	1.000000	74.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.911615	68489	69823	1429-1456	1.000000	74.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	20	20	45.0	50.0	57.1	58.1	10.9	14.6	0.930297	68489	69823	1479-1506	1.000000	73.8
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAAGACCGGAGCTATCAACC	ACCAACCATCAGAAGAAGCA	20	20	55.0	45.0	58.1	57.0	9.5	0.0	1.016077	78117	78445	465-465	1.000000	72.7
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	CTATCAACCACTCGGCCATC	ACCAACCATCAGAAGAAGCA	20	20	55.0	45.0	58.1	57.0	2.2	0.0	1.086322	78117	78445	454-454	1.000000	72.2
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	CAAGACCGGAGCTATCAACC	GACCAACCATCAGAAGAAGCA	20	21	55.0	47.6	58.1	58.2	9.5	0.0	1.275540	78117	78445	466-466	1.000000	70.7
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CAAGACCGGAGCTATCAACC	TGACCAACCATCAGAAGAAGC	20	21	55.0	47.6	58.1	58.2	9.5	0.0	1.275540	78117	78445	467-467	1.000000	70.7
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAAGACCGGAGCTATCAACC	ACCAACCATCAGAAGAAGCAA	20	21	55.0	42.9	58.1	57.8	9.5	0.0	1.286863	78117	78445	465-465	1.000000	70.6
accD_p1	accD	1	TCAAATAGGGGGCAGGAAGA	ACAAAATAACTGAATCTAGTGCT	20	23	50.0	30.4	58.0	54.0	0.0	0.0	6.996270	99934	101400	1453-1519	1.000000	45.1
accD_p2	accD	2	TCAAATAGGGGGCAGGAAGA	ACAAACAAAATAACTGAATCTAGTGC	20	26	50.0	30.8	58.0	57.0	0.0	0.0	7.045683	99934	101400	1457-1523	1.000000	45.1
accD_p3	accD	3	TCATCAAATAGGGGGCAGGA	ACAAAATAACTGAATCTAGTGCT	20	23	50.0	30.4	58.1	54.0	0.0	0.0	7.070241	99934	101400	1456-1522	1.000000	45.1
accD_p4	accD	4	TCATCAAATAGGGGGCAGGA	ACAAACAAAATAACTGAATCTAGTGC	20	26	50.0	30.8	58.1	57.0	0.0	0.0	7.119653	99934	101400	1460-1526	1.000000	45.1
accD_p5	accD	5	TCAAATAGGGGGCAGGAAGA	ACAAACAAAATAACTGAATCTAGTGCT	20	27	50.0	29.6	58.0	58.1	0.0	0.0	7.170034	99934	101400	1457-1523	1.000000	45.1
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	105854	106986	1247-1259	1.000000	81.3
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	105854	106986	1245-1257	1.000000	81.3
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.461827	105854	106986	1246-1258	1.000000	78.3
petA-psbJ_p4	petA-psbJ	4	GCCTTTTGTTCTTCTTCGCA	TCTGGGTTGGGTTCATCTCT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.819231	105854	106986	1246-1258	1.000000	75.5
petA-psbJ_p5	petA-psbJ	5	GCCTTTTGTTCTTCTTCGCA	TGGGTTGGGTTCATCTCTCT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.819231	105854	106986	1244-1256	1.000000	75.5
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	107756	109285	1654-1677	1.000000	76.9
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	107756	109285	1653-1676	1.000000	76.9
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	107756	109285	1667-1690	1.000000	76.6
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.745897	107756	109285	1666-1689	1.000000	76.6
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	20	20	45.0	45.0	58.2	57.3	0.0	0.0	0.827968	107756	109285	1662-1685	1.000000	76.0
rpl22-rps19_p1	rpl22-rps19	1	ACTTCTTTACCTGAACTTGAGT	TCTGACTTTCGCGAGACATG	22	20	36.4	50.0	55.0	58.0	0.0	9.6	5.051241	127482	127600	193-200	1.000000	44.5
rpl22-rps19_p2	rpl22-rps19	2	ACTTCTTTACCTGAACTTGAGTT	TCTGACTTTCGCGAGACATG	23	20	34.8	50.0	55.7	58.0	0.0	9.6	5.292253	127482	127600	193-200	1.000000	44.5
rpl22-rps19_p3	rpl22-rps19	3	ACTTCTTTACCTGAACTTGAGT	CTCTGACTTTCGCGAGACAT	22	20	36.4	50.0	55.0	57.7	0.0	9.6	5.304128	127482	127600	194-201	1.000000	44.4
rpl22-rps19_p4	rpl22-rps19	4	ACTTCTTTACCTGAACTTGAGT	CCTACTCTGACTTTCGCGAG	22	20	36.4	55.0	55.0	57.8	0.0	9.6	5.215279	127482	127600	198-205	1.000000	44.3
rpl22-rps19_p5	rpl22-rps19	5	ACTTCTTTACCTGAACTTGAGTT	CCTACTCTGACTTTCGCGAG	23	20	34.8	55.0	55.7	57.8	0.0	9.6	5.456291	127482	127600	198-205	1.000000	44.3
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	142587	144299	1870-1870	1.000000	77.1
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	142587	144299	1809-1809	1.000000	76.8
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	142587	144299	1813-1813	1.000000	76.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	142587	144299	1808-1808	1.000000	76.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	142587	144299	1811-1811	1.000000	75.8
rrn16S-trnI-GAU_p1	rrn16S-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	146094	146409	410-410	1.000000	78.9
rrn16S-trnI-GAU_p2	rrn16S-trnI-GAU	2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	146094	146409	442-442	1.000000	78.8
rrn16S-trnI-GAU_p3	rrn16S-trnI-GAU	3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.7	57.8	7.8	4.7	0.464916	146094	146409	461-461	1.000000	78.7
rrn16S-trnI-GAU_p4	rrn16S-trnI-GAU	4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	58.3	57.8	0.0	0.0	0.473195	146094	146409	464-464	1.000000	78.6
rrn16S-trnI-GAU_p5	rrn16S-trnI-GAU	5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	20	20	50.0	50.0	58.3	57.8	0.0	4.7	0.476037	146094	146409	483-483	1.000000	78.5
ycf1-ndhF_p1	ycf1-ndhF	1	TCTGGTTTGAAAAACCTTTTCT	TATGGGAGGTGGACGAATCT	22	20	31.8	50.0	54.6	57.5	19.7	0.0	5.898358	154232	154264	120-148	0.666667	32.5
ycf1-ndhF_p2	ycf1-ndhF	2	TCTGGTTTGAAAAACCTTTTCTT	TATGGGAGGTGGACGAATCT	23	20	30.4	50.0	55.3	57.5	19.7	0.0	6.123109	154232	154264	120-148	0.666667	32.5
ycf1-ndhF_p3	ycf1-ndhF	3	TCTGGTTTGAAAAACCTTTTCT	ATGGGAGGTGGACGAATCTC	22	20	31.8	55.0	54.6	58.9	19.7	0.0	6.302615	154232	154264	119-147	0.666667	32.5
ycf1-ndhF_p4	ycf1-ndhF	4	TTCTGGTTTGAAAAACCTTTTCT	TATGGGAGGTGGACGAATCT	23	20	30.4	50.0	55.3	57.5	19.7	0.0	6.123109	154232	154264	121-149	0.666667	32.4
ycf1-ndhF_p5	ycf1-ndhF	5	TTCTGGTTTGAAAAACCTTTTCTT	TATGGGAGGTGGACGAATCT	24	20	29.2	50.0	56.1	57.5	19.7	0.0	6.412666	154232	154264	121-149	0.666667	32.4