primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
MW450_pgt006-atpA_p1	MW450_pgt006-atpA	1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	21	20	42.9	50.0	57.4	58.4	5.3	0.0	1.948668	10321	11157	531-1006	1.000000	75.1
MW450_pgt006-atpA_p2	MW450_pgt006-atpA	2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	21	20	42.9	50.0	57.4	58.4	5.3	0.0	1.950350	10321	11157	530-1005	1.000000	75.1
MW450_pgt006-atpA_p3	MW450_pgt006-atpA	3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	21	20	47.6	50.0	57.3	58.4	0.0	0.0	2.067175	10321	11157	470-945	1.000000	74.7
MW450_pgt006-atpA_p4	MW450_pgt006-atpA	4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	21	20	47.6	50.0	56.9	58.4	0.0	0.0	2.425989	10321	11157	505-980	1.000000	71.5
MW450_pgt006-atpA_p5	MW450_pgt006-atpA	5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	20	20	40.0	50.0	55.9	58.4	0.0	0.0	2.453105	10321	11157	531-1006	1.000000	71.1
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	14720	15895	1257-1283	1.000000	81.1
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14720	15895	1223-1249	1.000000	81.1
atpH-atpI_p3	atpH-atpI	3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14720	15895	1287-1313	1.000000	80.2
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14720	15895	1253-1279	1.000000	80.2
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14720	15895	1224-1250	1.000000	79.9
rpoB-MW450_pgt007_p1	rpoB-MW450_pgt007	1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.210899	28183	29397	1358-1373	1.000000	79.9
rpoB-MW450_pgt007_p2	rpoB-MW450_pgt007	2	TAAGCCCTGCTCAATGAACC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.9	58.4	0.0	14.6	0.484261	28183	29397	1340-1355	1.000000	77.7
rpoB-MW450_pgt007_p3	rpoB-MW450_pgt007	3	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.534728	28183	29397	1292-1307	1.000000	77.4
rpoB-MW450_pgt007_p4	rpoB-MW450_pgt007	4	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.561694	28183	29397	1364-1379	1.000000	77.1
rpoB-MW450_pgt007_p5	rpoB-MW450_pgt007	5	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.5	58.1	0.0	0.0	0.627927	28183	29397	1368-1383	1.000000	76.5
petN-psbM_p1	petN-psbM	1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.465211	30492	31707	1299-1324	1.000000	78.7
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30492	31707	1242-1267	1.000000	78.1
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30492	31707	1241-1266	1.000000	78.1
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.8	57.6	0.0	0.0	0.611620	30492	31707	1240-1265	1.000000	77.6
petN-psbM_p5	petN-psbM	5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	20	20	60.0	45.0	58.2	57.6	6.7	0.0	0.652301	30492	31707	1237-1262	1.000000	77.3
psbM-MW450_pgt008_p1	psbM-MW450_pgt008	1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.6	58.5	0.0	5.4	0.912704	31813	33040	1391-1418	1.000000	74.2
psbM-MW450_pgt008_p2	psbM-MW450_pgt008	2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31813	33040	1393-1420	1.000000	72.4
psbM-MW450_pgt008_p3	psbM-MW450_pgt008	3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31813	33040	1392-1419	1.000000	72.4
psbM-MW450_pgt008_p4	psbM-MW450_pgt008	4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	57.6	57.0	0.0	5.4	1.432858	31813	33040	1392-1419	1.000000	70.0
psbM-MW450_pgt008_p5	psbM-MW450_pgt008	5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	58.1	58.5	0.0	5.4	1.527884	31813	33040	1392-1419	1.000000	69.3
MW450_pgt011-psbD_p1	MW450_pgt011-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	34372	35781	1575-1713	1.000000	82.9
MW450_pgt011-psbD_p2	MW450_pgt011-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	34372	35781	1578-1716	1.000000	82.7
MW450_pgt011-psbD_p3	MW450_pgt011-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	34372	35781	1577-1715	1.000000	82.7
MW450_pgt011-psbD_p4	MW450_pgt011-psbD	4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	34372	35781	1565-1703	1.000000	82.7
MW450_pgt011-psbD_p5	MW450_pgt011-psbD	5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	34372	35781	1580-1718	1.000000	82.5
MW450_pgt012-MW450_pgt013_p1	MW450_pgt012-MW450_pgt013	1	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.0	57.8	0.0	1.1	1.188961	39109	39465	483-574	1.000000	75.7
MW450_pgt012-MW450_pgt013_p2	MW450_pgt012-MW450_pgt013	2	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	57.8	57.8	0.0	1.1	1.376876	39109	39465	456-547	1.000000	74.2
MW450_pgt012-MW450_pgt013_p3	MW450_pgt012-MW450_pgt013	3	TCCGGCACATCATTATGGAT	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	56.7	57.8	0.0	1.1	1.420491	39109	39465	482-573	1.000000	73.8
MW450_pgt012-MW450_pgt013_p4	MW450_pgt012-MW450_pgt013	4	TTTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	21	20	42.9	50.0	57.7	57.8	0.0	1.1	1.459239	39109	39465	484-575	1.000000	73.5
MW450_pgt012-MW450_pgt013_p5	MW450_pgt012-MW450_pgt013	5	TCCGGCACATCATTATGGATT	CGTCTTCTCCTTGGCAAAGA	21	20	42.9	50.0	57.5	57.8	0.0	1.1	1.676994	39109	39465	482-573	1.000000	71.8
psaA-pafI_p1	psaA-pafI	1	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCAATAGCGCT	20	20	50.0	45.0	57.9	58.5	4.1	17.3	0.581718	44843	45549	862-912	1.000000	77.6
psaA-pafI_p2	psaA-pafI	2	GCCCATTCCTCGAAAGAAGT	CAATAGCGCTTACTCCTGGT	20	20	50.0	50.0	57.9	57.4	4.1	18.7	0.719389	44843	45549	852-902	1.000000	76.5
psaA-pafI_p3	psaA-pafI	3	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	20	20	50.0	55.0	57.9	58.6	4.1	18.9	0.741772	44843	45549	893-943	1.000000	76.2
psaA-pafI_p4	psaA-pafI	4	GCCCATTCCTCGAAAGAAGT	GCAATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.9	58.8	4.1	18.7	0.893928	44843	45549	853-903	1.000000	75.1
psaA-pafI_p5	psaA-pafI	5	TTGAGAAATGACCCGGTCTG	TGGAAACAAGCAATAGCGCT	20	20	50.0	45.0	57.5	58.5	18.5	17.3	0.939070	44843	45549	882-932	1.000000	74.6
MW450_pgt018-MW450_pgt019_p1	MW450_pgt018-MW450_pgt019	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49562	50780	1241-1320	1.000000	85.3
MW450_pgt018-MW450_pgt019_p2	MW450_pgt018-MW450_pgt019	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49562	50780	1249-1328	1.000000	84.8
MW450_pgt018-MW450_pgt019_p3	MW450_pgt018-MW450_pgt019	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49562	50780	1273-1352	1.000000	84.4
MW450_pgt018-MW450_pgt019_p4	MW450_pgt018-MW450_pgt019	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	49562	50780	1281-1360	1.000000	83.9
MW450_pgt018-MW450_pgt019_p5	MW450_pgt018-MW450_pgt019	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	49562	50780	1255-1334	1.000000	83.4
ndhC-MW450_pgt021_p1	ndhC-MW450_pgt021	1	GGGGCTAAAACTCCGGAAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	5.2	0.0	0.550649	54251	55753	1399-1721	1.000000	82.3
ndhC-MW450_pgt021_p2	ndhC-MW450_pgt021	2	CTGGTCCTTTGCTAATCGGG	CCCGCCGTATGAAAGCAATA	20	20	55.0	50.0	58.3	58.4	0.0	0.0	0.742907	54251	55753	1416-1738	1.000000	80.7
ndhC-MW450_pgt021_p3	ndhC-MW450_pgt021	3	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.753172	54251	55753	1411-1733	1.000000	80.6
ndhC-MW450_pgt021_p4	ndhC-MW450_pgt021	4	GGGGCTAAAACTCCGGAAAT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	5.2	0.0	0.802602	54251	55753	1398-1720	1.000000	80.3
ndhC-MW450_pgt021_p5	ndhC-MW450_pgt021	5	GGGGCTAAAACTCCGGAAAT	TTTGAACCAATGACTCCCGC	20	20	50.0	50.0	57.9	58.8	5.2	0.0	0.895421	54251	55753	1414-1736	1.000000	79.5
accD-psaI_p1	accD-psaI	1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.4	58.0	0.0	26.9	0.397961	62246	62828	715-1123	0.967742	80.9
accD-psaI_p2	accD-psaI	2	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.415750	62246	62828	672-1080	0.967742	80.8
accD-psaI_p3	accD-psaI	3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	58.4	58.0	0.0	5.0	0.417959	62246	62828	706-1114	0.967742	80.8
accD-psaI_p4	accD-psaI	4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	20	20	50.0	55.0	58.4	58.1	0.0	0.0	0.439322	62246	62828	668-1076	0.967742	80.7
accD-psaI_p5	accD-psaI	5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.447602	62246	62828	679-1087	0.967742	80.6
petA-psbJ_p1	petA-psbJ	1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66306	67297	1158-1207	1.000000	76.4
petA-psbJ_p2	petA-psbJ	2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66306	67297	1159-1208	1.000000	76.4
petA-psbJ_p3	petA-psbJ	3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	58.7	57.8	0.0	1.9	0.916070	66306	67297	1174-1223	1.000000	75.4
petA-psbJ_p4	petA-psbJ	4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.3	57.8	0.0	1.9	0.938964	66306	67297	1165-1214	1.000000	75.3
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.2	57.8	0.0	1.9	0.980015	66306	67297	1181-1230	1.000000	74.9
petG-MW450_pgt022_p1	petG-MW450_pgt022	1	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	20	20	50.0	50.0	57.8	57.9	0.0	5.5	0.262804	69680	69803	173-173	1.000000	87.1
petG-MW450_pgt022_p2	petG-MW450_pgt022	2	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.264278	69680	69803	174-174	1.000000	87.1
petG-MW450_pgt022_p3	petG-MW450_pgt022	3	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.243803	69680	69803	192-192	1.000000	86.4
petG-MW450_pgt022_p4	petG-MW450_pgt022	4	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.191682	69680	69803	206-206	1.000000	86.2
petG-MW450_pgt022_p5	petG-MW450_pgt022	5	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.170881	69680	69803	228-228	1.000000	85.7
rpl16_p1	rpl16	1	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTT	20	20	45.0	35.0	57.8	53.3	0.0	0.0	4.888278	84954	86361	1519-1601	1.000000	43.4
rpl16_p2	rpl16	2	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTTT	20	21	45.0	33.3	57.8	54.2	0.0	0.0	4.982512	84954	86361	1519-1601	1.000000	42.6
rpl16_p3	rpl16	3	ATCCCAAAAACCCCTTTCGT	TGGCAAACTCTTTCATTCTT	20	20	45.0	35.0	57.6	53.3	0.0	0.0	5.117941	84954	86361	1520-1602	1.000000	42.5
rpl16_p4	rpl16	4	TCCCAAAAACCCCTTTCGTT	ATGGCAAACTCTTTCATTCT	20	20	45.0	35.0	57.8	53.0	0.0	0.0	5.134415	84954	86361	1520-1602	1.000000	42.5
rpl16_p5	rpl16	5	TCCCAAAAACCCCTTTCGTT	TGGCAAACTCTTTCATTCTTTT	20	22	45.0	31.8	57.8	55.0	0.0	0.0	5.158877	84954	86361	1519-1601	1.000000	42.5
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	20	20	40.0	45.0	57.0	57.8	0.0	13.1	1.214860	116579	117639	1225-1317	1.000000	76.3
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	20	20	40.0	45.0	57.0	57.8	0.0	0.0	1.261149	116579	117639	1223-1315	1.000000	75.9
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	20	20	40.0	55.0	57.0	58.3	0.0	0.0	1.377810	116579	117639	1212-1304	1.000000	75.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	20	20	40.0	45.0	57.0	57.6	0.0	0.0	1.489633	116579	117639	1222-1314	1.000000	74.1
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	21	20	38.1	45.0	57.7	57.8	0.0	13.1	1.495037	116579	117639	1226-1318	1.000000	74.1