primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	75	459	448-451	1.000000	81.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	75	459	498-501	1.000000	81.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	75	459	512-515	1.000000	81.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	75	459	511-514	1.000000	81.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	75	459	513-516	1.000000	81.3
matK_p1	matK	1	TTTCCATTTCCACGGTCACA	ACCTTGTTTTGACTGTATCGT	20	21	45.0	38.1	57.6	55.5	0.0	0.0	3.885900	2079	3590	1631-1631	1.000000	49.2
matK_p2	matK	2	TTCCATTTCCACGGTCACAT	ACCTTGTTTTGACTGTATCGT	20	21	45.0	38.1	57.4	55.5	0.0	0.0	4.113449	2079	3590	1630-1630	1.000000	47.4
matK_p3	matK	3	TTCCACGGTCACATAACCAA	ACCTTGTTTTGACTGTATCGT	20	21	45.0	38.1	57.1	55.5	0.0	0.0	4.468697	2079	3590	1624-1624	1.000000	44.5
matK_p4	matK	4	TTTCCACGGTCACATAACCA	ACCTTGTTTTGACTGTATCGT	20	21	45.0	38.1	57.1	55.5	0.0	0.0	4.468697	2079	3590	1625-1625	1.000000	44.5
matK_p5	matK	5	TTTCCATTTCCACGGTCACA	ACCTTGTTTTGACTGTATCGTACT	20	24	45.0	37.5	57.6	58.1	0.0	0.0	4.499443	2079	3590	1631-1631	1.000000	44.3
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	20	20	50.0	50.0	57.9	57.1	1.4	0.0	0.989589	8022	8278	388-394	1.000000	74.3
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	8022	8278	385-391	1.000000	72.3
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	8022	8278	386-392	1.000000	72.3
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	8022	8278	387-393	1.000000	72.3
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTACG	20	21	50.0	47.6	57.9	57.8	1.4	0.0	1.282440	8022	8278	389-395	1.000000	72.0
petN-psbM_p1	petN-psbM	1	ACATTTTCCCTTTCGCTCGT	AAAACAGTCAGCCAAGGTGA	20	20	45.0	45.0	58.1	57.6	0.0	0.0	0.543163	29971	30810	909-909	1.000000	76.0
petN-psbM_p2	petN-psbM	2	CCCTTTCGCTCGTAGTATGG	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.9	57.6	0.0	0.0	0.571037	29971	30810	902-902	1.000000	75.8
petN-psbM_p3	petN-psbM	3	CCTTTCGCTCGTAGTATGGG	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.9	57.6	0.0	0.0	0.571037	29971	30810	901-901	1.000000	75.8
petN-psbM_p4	petN-psbM	4	CTTTCGCTCGTAGTATGGGG	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.9	57.6	0.0	0.0	0.571037	29971	30810	900-900	1.000000	75.8
petN-psbM_p5	petN-psbM	5	TTCGCTCGTAGTATGGGGAA	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.668879	29971	30810	898-898	1.000000	75.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	33400	34689	1454-1465	1.000000	81.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	33400	34689	1457-1468	1.000000	81.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	33400	34689	1456-1467	1.000000	81.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	33400	34689	1459-1470	1.000000	81.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.293100	33400	34689	1431-1442	1.000000	79.8
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	AAGGAGAGAGAGGGATTCGA	CAACCCTCAGGAGAAGCAAA	20	20	50.0	50.0	56.9	57.7	0.0	0.0	1.411229	37440	37821	570-574	1.000000	70.0
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	AGGAGAGAGAGGGATTCGAA	CAACCCTCAGGAGAAGCAAA	20	20	50.0	50.0	56.9	57.7	0.0	0.0	1.411229	37440	37821	569-573	1.000000	70.0
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	AAGGAGAGAGAGGGATTCGA	TTTTGTTACCCGACCAACCC	20	20	50.0	50.0	56.9	58.3	0.0	0.0	1.444513	37440	37821	584-588	1.000000	69.7
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	AGGAGAGAGAGGGATTCGAA	TTTTGTTACCCGACCAACCC	20	20	50.0	50.0	56.9	58.3	0.0	0.0	1.444513	37440	37821	583-587	1.000000	69.7
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	GAGAGAGAGGGATTCGAACC	CAACCCTCAGGAGAAGCAAA	20	20	55.0	50.0	56.8	57.7	0.0	0.0	1.445927	37440	37821	567-571	1.000000	69.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTTGCTTCTCCTGAGGGTTG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.335407	38011	38522	600-681	1.000000	82.4
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTTGCTTCTCCTGAGGGTTG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.335407	38011	38522	601-682	1.000000	82.4
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GGGTTGGTCGGGTAACAAAA	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.368691	38011	38522	586-667	1.000000	82.2
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GGGTTGGTCGGGTAACAAAA	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.368691	38011	38522	587-668	1.000000	82.2
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTTGCTTCTCCTGAGGGTTG	GAACCCGCATCTTCTCCTTG	20	20	50.0	55.0	57.7	58.6	0.0	0.0	0.903162	38011	38522	596-677	1.000000	77.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48607	49914	1312-1358	1.000000	82.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	48607	49914	1357-1403	1.000000	81.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48607	49914	1320-1366	1.000000	81.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	48607	49914	1365-1411	1.000000	81.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48607	49914	1344-1390	1.000000	81.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	58.2	5.2	0.0	0.304202	53415	54136	856-917	1.000000	81.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	53415	54136	792-853	1.000000	81.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	53415	54136	859-920	1.000000	80.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	53415	54136	858-919	1.000000	80.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	20	20	55.0	50.0	58.3	58.2	0.0	0.0	0.496460	53415	54136	873-934	1.000000	80.4
rbcL-accD_p1	rbcL-accD	1	GAGTCCTGAACTAGCTGCTG	CGAATCGAACCGCCATTTTT	20	20	55.0	45.0	57.7	57.7	13.4	0.0	0.548547	59407	60121	798-815	1.000000	78.2
rbcL-accD_p2	rbcL-accD	2	GAGTCCTGAACTAGCTGCTG	ATCGAATCGAACCGCCATTT	20	20	55.0	45.0	57.7	58.3	13.4	10.1	0.628408	59407	60121	800-817	1.000000	77.5
rbcL-accD_p3	rbcL-accD	3	GAGTCCTGAACTAGCTGCTG	ATCGAACCGCCATTTTTCCA	20	20	55.0	45.0	57.7	58.5	13.4	0.0	0.751836	59407	60121	795-812	1.000000	76.6
rbcL-accD_p4	rbcL-accD	4	GAGTCCTGAACTAGCTGCTG	TCGAACCGCCATTTTTCCAT	20	20	55.0	45.0	57.7	58.5	13.4	0.0	0.751836	59407	60121	794-811	1.000000	76.6
rbcL-accD_p5	rbcL-accD	5	GCTGCTGCTTGTGAAGTATG	CGAATCGAACCGCCATTTTT	20	20	50.0	45.0	57.5	57.7	8.5	0.0	0.795791	59407	60121	785-802	1.000000	76.3
accD-psaI_p1	accD-psaI	1	GATCCAATCGTACCGCGTAA	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.9	58.0	4.9	5.0	0.171013	61583	62375	938-949	1.000000	82.3
accD-psaI_p2	accD-psaI	2	TCCAATCGTACCGCGTAATC	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.9	58.0	4.9	5.0	0.171013	61583	62375	936-947	1.000000	82.3
accD-psaI_p3	accD-psaI	3	CCAATCGTACCGCGTAATCT	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	57.9	58.0	4.9	5.0	0.176825	61583	62375	935-946	1.000000	82.2
accD-psaI_p4	accD-psaI	4	GATCCAATCGTACCGCGTAA	AAATGGAGGGGAAGCTGTTG	20	20	50.0	50.0	57.9	58.1	4.9	0.0	0.195143	61583	62375	905-916	1.000000	82.2
accD-psaI_p5	accD-psaI	5	TCCAATCGTACCGCGTAATC	AAATGGAGGGGAAGCTGTTG	20	20	50.0	50.0	57.9	58.1	4.9	0.0	0.195143	61583	62375	903-914	1.000000	82.2
psaJ-rpl33_p1	psaJ-rpl33	1	ACATATCTTTCCGTGGCACC	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	58.0	57.0	12.9	0.0	1.029127	70223	70748	648-667	1.000000	74.4
psaJ-rpl33_p2	psaJ-rpl33	2	TCCGGATGCGTTGATATTCC	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	0.0	0.0	1.154558	70223	70748	565-584	1.000000	73.8
psaJ-rpl33_p3	psaJ-rpl33	3	GTTCGGGTCTTTAGCAGGTT	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	0.0	0.0	1.171172	70223	70748	610-629	1.000000	73.5
psaJ-rpl33_p4	psaJ-rpl33	4	ACATATCTTTCCGTGGCACC	CACGATTTCGAACACAACTGG	20	21	50.0	47.6	58.0	58.1	12.9	7.0	1.134709	70223	70748	694-713	1.000000	73.4
psaJ-rpl33_p5	psaJ-rpl33	5	TGGCACCGGTAGTAAGTACT	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	6.9	0.0	1.211393	70223	70748	635-654	1.000000	73.0
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	GAGGTTAAGCGTACTCTGGC	GATGACTTCCACCACGTCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.239919	102145	103847	1810-1815	1.000000	78.7
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	GAGGTTAAGCGTACTCTGGC	ATGACTTCCACCACGTCAAG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.245933	102145	103847	1809-1814	1.000000	78.7
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GAGGTTAAGCGTACTCTGGC	AACTGATGACTTCCACCACG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.245933	102145	103847	1814-1819	1.000000	78.6
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GGTTAAGCGTACTCTGGCAA	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.252768	102145	103847	1808-1813	1.000000	78.6
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	GGTTAAGCGTACTCTGGCAA	ATGACTTCCACCACGTCAAG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.258782	102145	103847	1807-1812	1.000000	78.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCGTTGAAAGCCTTTTCGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.172709	117279	118179	1000-1014	1.000000	79.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCGTTGAAAGCCTTTTCGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.174080	117279	118179	1001-1015	1.000000	79.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGTTGAAAGCCTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.289156	117279	118179	1010-1024	1.000000	78.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGTTGAAAGCCTTTTCGTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.361174	117279	118179	1009-1023	1.000000	78.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGTTGAAAGCCTTTTCGTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.361174	117279	118179	1008-1022	1.000000	78.4
ndhD-psaC_p1	ndhD-psaC	1	AAAATTAAGGAACCCGCGGA	AAGAGATGTGAATCCGCCTG	20	20	45.0	50.0	57.8	57.7	30.7	0.0	0.525826	121115	121247	286-291	1.000000	77.7
ndhD-psaC_p2	ndhD-psaC	2	AAAATTAAGGAACCCGCGGA	CAGAGGACTGTGTTGGTTGT	20	20	45.0	50.0	57.8	57.7	30.7	0.0	0.535534	121115	121247	306-311	1.000000	77.5
ndhD-psaC_p3	ndhD-psaC	3	AAAATTAAGGAACCCGCGGA	GAGATGTGAATCCGCCTGTC	20	20	45.0	55.0	57.8	58.4	30.7	0.0	0.627115	121115	121247	284-289	1.000000	76.9
ndhD-psaC_p4	ndhD-psaC	4	AAAATTAAGGAACCCGCGGA	GCCTGTCCAACGGATTTCTT	20	20	45.0	50.0	57.8	58.5	30.7	0.0	0.668742	121115	121247	271-276	1.000000	76.7
ndhD-psaC_p5	ndhD-psaC	5	AAATTAAGGAACCCGCGGAT	AAGAGATGTGAATCCGCCTG	20	20	45.0	50.0	57.6	57.7	30.7	0.0	0.752603	121115	121247	285-290	1.000000	75.9
ycf1_p1	ycf1	1	TGTTTTACGTCGTTGAGCAC	ACCGAGGAAAGTGTGGTAGA	20	20	45.0	50.0	57.0	58.0	1.8	0.0	1.008000	131564	131683	222-222	1.000000	72.7
ycf1_p2	ycf1	2	TGTTTTACGTCGTTGAGCAC	GAAACCGAGGAAAGTGTGGT	20	20	45.0	50.0	57.0	58.0	1.8	0.0	1.039458	131564	131683	225-225	1.000000	72.4
ycf1_p3	ycf1	3	CTGTTTTACGTCGTTGAGCAC	ACCGAGGAAAGTGTGGTAGA	21	20	47.6	50.0	58.1	58.0	1.8	0.0	1.117637	131564	131683	223-223	1.000000	71.8
ycf1_p4	ycf1	4	CTGTTTTACGTCGTTGAGCAC	GAAACCGAGGAAAGTGTGGT	21	20	47.6	50.0	58.1	58.0	1.8	0.0	1.149094	131564	131683	226-226	1.000000	71.5
ycf1_p5	ycf1	5	TGTTTTACGTCGTTGAGCAC	TGGAACAACAGGAGGGAGAA	20	20	45.0	50.0	57.0	58.2	1.8	0.0	1.188201	131564	131683	242-242	1.000000	71.2
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GATGACTTCCACCACGTCAA	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.239919	142713	144415	1810-1815	1.000000	78.7
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	ATGACTTCCACCACGTCAAG	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.245933	142713	144415	1809-1814	1.000000	78.7
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	GAGGTTAAGCGTACTCTGGC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.245933	142713	144415	1814-1819	1.000000	78.6
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	GATGACTTCCACCACGTCAA	GGTTAAGCGTACTCTGGCAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.252768	142713	144415	1808-1813	1.000000	78.6
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	ATGACTTCCACCACGTCAAG	GGTTAAGCGTACTCTGGCAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.258782	142713	144415	1807-1812	1.000000	78.5