primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	AACACCAAACCATCCGATGT	CGGTTTGGGGAGGGATTTTT	20	20	45.0	50.0	57.7	58.4	7.6	0.0	0.646642	1833	2389	768-769	1.000000	83.2
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	ACGGTTTTCAGTGCTGGTTA	CGGTTTGGGGAGGGATTTTT	20	20	45.0	50.0	57.7	58.4	0.0	0.0	0.695594	1833	2389	744-745	1.000000	82.9
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	AACACCAAACCATCCGATGT	AAAATGCAAGCACGGTTTGG	20	20	45.0	45.0	57.7	58.4	7.6	0.0	0.705549	1833	2389	780-781	1.000000	82.7
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	20	20	45.0	45.0	57.7	58.4	0.0	0.0	0.754500	1833	2389	756-757	1.000000	82.4
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	7.6	0.0	0.895799	1833	2389	704-705	1.000000	81.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4980	5920	1032-1069	1.000000	83.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4980	5920	1031-1068	1.000000	83.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	57.5	58.0	0.0	17.2	0.473875	4980	5920	1018-1055	1.000000	80.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4980	5920	992-1029	1.000000	80.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4980	5920	991-1028	1.000000	80.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.080725	7065	7634	686-780	1.000000	83.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	7065	7634	643-737	1.000000	82.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	7065	7634	654-748	1.000000	82.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.274928	7065	7634	677-771	1.000000	82.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTTGACATCCACAGCCGATC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	0.0	16.9	0.338263	7065	7634	676-770	1.000000	81.5
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044251	14159	14820	747-785	1.000000	83.8
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124357	14159	14820	775-813	1.000000	83.0
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.500159	14159	14820	748-786	1.000000	80.1
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.560653	14159	14820	753-791	1.000000	79.6
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.580266	14159	14820	776-814	1.000000	79.4
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155684	49991	50735	905-971	1.000000	84.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.196808	49991	50735	909-975	1.000000	83.9
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TAAGATCGCTTCCGGCTCTA	TTGAGTTTGAGCCCGCTTAG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.214533	49991	50735	925-991	1.000000	83.7
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263437	49991	50735	873-939	1.000000	83.6
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTTCTAAGATCGCTTCCGGC	TTGAGTTTGAGCCCGCTTAG	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.255657	49991	50735	929-995	1.000000	83.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50809	51883	1062-1125	1.000000	83.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50809	51883	1070-1133	1.000000	82.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50809	51883	1094-1157	1.000000	82.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	50809	51883	1102-1165	1.000000	81.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CAAATAGGGAAAGCCGGCTA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	33.1	0.0	0.346470	50809	51883	1116-1179	1.000000	81.7
ndhC-trnM-CAU_p1	ndhC-trnM-CAU	1	ACCCTTTCTAATCGGGGCTA	AGACACCGGGATCAATGGTA	20	20	50.0	50.0	57.8	58.1	0.0	6.3	0.311533	55489	57466	2194-2225	1.000000	79.2
ndhC-trnM-CAU_p2	ndhC-trnM-CAU	2	ACCCTTTCTAATCGGGGCTA	TAGACACCGGGATCAATGGT	20	20	50.0	50.0	57.8	58.1	0.0	6.3	0.311533	55489	57466	2195-2226	1.000000	79.2
ndhC-trnM-CAU_p3	ndhC-trnM-CAU	3	CGGGGCTAAAACTCCAGAAA	AGACACCGGGATCAATGGTA	20	20	50.0	50.0	57.8	58.1	0.0	6.3	0.320072	55489	57466	2182-2213	1.000000	79.2
ndhC-trnM-CAU_p4	ndhC-trnM-CAU	4	CGGGGCTAAAACTCCAGAAA	TAGACACCGGGATCAATGGT	20	20	50.0	50.0	57.8	58.1	0.0	6.3	0.320072	55489	57466	2183-2214	1.000000	79.2
ndhC-trnM-CAU_p5	ndhC-trnM-CAU	5	ATCGGGGCTAAAACTCCAGA	AGACACCGGGATCAATGGTA	20	20	50.0	50.0	58.4	58.1	0.0	6.3	0.545811	55489	57466	2184-2215	1.000000	77.4
atpB-rbcL_p1	atpB-rbcL	1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.164621	59679	60680	1080-1163	1.000000	80.9
atpB-rbcL_p2	atpB-rbcL	2	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	58.0	58.4	0.0	0.0	0.465502	59679	60680	1081-1164	1.000000	78.5
atpB-rbcL_p3	atpB-rbcL	3	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	20	20	45.0	55.0	58.0	57.3	0.0	0.0	0.738828	59679	60680	1082-1165	1.000000	76.3
atpB-rbcL_p4	atpB-rbcL	4	TTTCAAGCGTGGAAACTCCA	AAGTCCCTCCCTACAACTCA	20	20	45.0	50.0	58.0	57.3	0.0	0.0	0.761324	59679	60680	1072-1155	1.000000	76.1
atpB-rbcL_p5	atpB-rbcL	5	TTTCAAGCGTGGAAACTCCA	AGTCCCTCCCTACAACTCAT	20	20	45.0	50.0	58.0	57.0	0.0	0.0	0.996551	59679	60680	1071-1154	1.000000	74.2
psaJ-rpl33_p1	psaJ-rpl33	1	TTCCCGGATGCGTTAACATT	ATACCTGGAAATGCCCGTTG	20	20	45.0	50.0	57.9	58.2	0.7	0.0	0.354119	73116	73637	631-654	1.000000	80.5
psaJ-rpl33_p2	psaJ-rpl33	2	GTTTCTTCCCGGATGCGTTA	ATACCTGGAAATGCCCGTTG	20	20	50.0	50.0	58.3	58.2	0.7	0.0	0.508009	73116	73637	636-659	1.000000	79.3
psaJ-rpl33_p3	psaJ-rpl33	3	TTCTTCCCGGATGCGTTAAC	ATACCTGGAAATGCCCGTTG	20	20	50.0	50.0	58.3	58.2	0.7	0.0	0.508009	73116	73637	634-657	1.000000	79.3
psaJ-rpl33_p4	psaJ-rpl33	4	CGGATGCGTTAACATTCCCT	ATACCTGGAAATGCCCGTTG	20	20	50.0	50.0	58.3	58.2	6.4	0.0	0.573598	73116	73637	627-650	1.000000	78.8
psaJ-rpl33_p5	psaJ-rpl33	5	CTTCCCGGATGCGTTAACAT	ATACCTGGAAATGCCCGTTG	20	20	50.0	50.0	58.3	58.2	0.7	0.0	0.573598	73116	73637	632-655	1.000000	78.8
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.790741	73839	74421	756-774	1.000000	80.0
rpl33-rps18_p2	rpl33-rps18	2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	73839	74421	755-773	1.000000	77.6
rpl33-rps18_p3	rpl33-rps18	3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	73839	74421	757-775	1.000000	77.5
rpl33-rps18_p4	rpl33-rps18	4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.5	58.6	0.0	0.0	1.173970	73839	74421	753-771	1.000000	76.9
rpl33-rps18_p5	rpl33-rps18	5	ACGCCTAGTCGATTGGAATT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.236405	73839	74421	747-765	1.000000	76.4
rpl22-rps19_p1	rpl22-rps19	1	AGTTGGATATGGGTTCCTCT	TTGGGAGAATTCGCACCTAC	20	20	45.0	50.0	55.2	57.6	0.0	9.1	3.219538	90822	91078	286-368	1.000000	61.1
rpl22-rps19_p2	rpl22-rps19	2	TCGTTTGTAGTTGGATATGGGT	TTGGGAGAATTCGCACCTAC	22	20	40.9	50.0	57.2	57.6	0.0	9.1	3.226462	90822	91078	294-376	1.000000	60.9
rpl22-rps19_p3	rpl22-rps19	3	AGTTGGATATGGGTTCCTCT	TGGGAGAATTCGCACCTACT	20	20	45.0	50.0	55.2	58.4	0.0	9.1	3.254445	90822	91078	285-367	1.000000	60.8
rpl22-rps19_p4	rpl22-rps19	4	TCGTTTGTAGTTGGATATGGGT	TGGGAGAATTCGCACCTACT	22	20	40.9	50.0	57.2	58.4	0.0	9.1	3.261368	90822	91078	293-375	1.000000	60.7
rpl22-rps19_p5	rpl22-rps19	5	AGTTGGATATGGGTTCCTCT	AATTGGGAGAATTCGCACCT	20	20	45.0	45.0	55.2	57.5	0.0	9.1	3.337407	90822	91078	288-370	1.000000	60.1
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	101544	102176	689-693	1.000000	80.2
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	101544	102176	687-691	1.000000	80.2
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.1	57.9	0.0	29.4	0.139265	101544	102176	677-681	1.000000	79.6
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	101544	102176	684-688	1.000000	78.7
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	57.7	57.9	5.8	29.4	0.330507	101544	102176	688-692	1.000000	78.1
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	116433	116979	784-794	1.000000	83.7
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	116433	116979	783-793	1.000000	83.7
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGGTTACCTGCTCTTAACGG	GGGGAAGTCGCATCTCTTTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135290	116433	116979	776-786	1.000000	83.6
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGCAGAATAGCAGAGCAAGT	ATATGTAGGGGGAAGTCGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132640	116433	116979	1090-1100	1.000000	82.3
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGCAGAATAGCAGAGCAAGT	TATGTAGGGGGAAGTCGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132640	116433	116979	1089-1099	1.000000	82.3
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCGTCGGAAAAAGTA	TTTCAACGCCGCCCAATATA	20	20	45.0	45.0	58.0	58.0	17.5	0.0	0.088990	119191	120469	757-1468	1.000000	91.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCGTCGGAAAAAGTA	AACGCCGCCCAATATACTTT	20	20	45.0	45.0	58.0	58.0	17.5	0.0	0.096174	119191	120469	753-1464	1.000000	91.2
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCCGTCGGAAAAAGTA	CGCCGCCCAATATACTTTCT	20	20	45.0	50.0	58.0	58.1	17.5	0.0	0.170219	119191	120469	751-1462	1.000000	90.6
ndhF-rpl32_p4	ndhF-rpl32	4	TGTTGCCGTCGGAAAAAGTA	TGAAAAAGCTTTCAACGCCG	20	20	45.0	45.0	58.0	58.2	17.5	13.1	0.215312	119191	120469	766-1477	1.000000	90.3
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTCGGAAAAAG	TTTCAACGCCGCCCAATATA	20	20	45.0	45.0	57.8	58.0	17.5	0.0	0.236113	119191	120469	759-1470	1.000000	90.1
ycf1_p1	ycf1	1	CTTGCTCCTGTTTATCCCCC	GATCTACTATGCGCCTCTGC	20	20	55.0	55.0	57.9	58.0	0.0	4.2	0.069162	136696	136815	689-692	1.000000	83.5
ycf1_p2	ycf1	2	CACTTTCCCCCACTTCTTCC	GATCTACTATGCGCCTCTGC	20	20	55.0	55.0	58.1	58.0	0.0	4.2	0.099512	136696	136815	626-629	1.000000	83.5
ycf1_p3	ycf1	3	CTTCGGTGGATACCTCTTGC	GATCTACTATGCGCCTCTGC	20	20	55.0	55.0	58.1	58.0	0.0	4.2	0.069245	136696	136815	704-707	1.000000	83.4
ycf1_p4	ycf1	4	GAAAGTGGGGTTCGTCCTTT	GATCTACTATGCGCCTCTGC	20	20	50.0	55.0	58.0	58.0	0.0	4.2	0.033513	136696	136815	821-824	1.000000	83.2
ycf1_p5	ycf1	5	GAAAGTGGGGTTCGTCCTTT	TTCGGTCGTTATGGTTGGAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106110	136696	136815	992-995	1.000000	82.1
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	TGTTTTTGTAAAGACGAAGAAAGA	20	24	50.0	29.2	58.3	55.5	13.0	0.0	6.800178	161281	162797	1620-1633	1.000000	40.5
rpl2_p2	rpl2	2	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCTC	20	24	50.0	33.3	58.3	55.4	13.0	11.9	6.841302	161281	162797	1609-1622	1.000000	40.5
rpl2_p3	rpl2	3	ATCATTACGCTTCAACCGGG	TGTTTTTGTAAAGACGAAGAAAGA	20	24	50.0	29.2	58.3	55.5	6.3	0.0	6.870646	161281	162797	1627-1640	1.000000	40.5
rpl2_p4	rpl2	4	ATCATTACGCTTCAACCGGG	AGACGAAGAAAGAAATTTTCTCTC	20	24	50.0	33.3	58.3	55.4	6.3	11.9	6.911771	161281	162797	1616-1629	1.000000	40.5
rpl2_p5	rpl2	5	CGCTTCAACCGGGTTATTCT	AGACGAAGAAAGAAATTTTCTCT	20	23	50.0	30.4	58.3	54.3	13.0	11.9	7.014201	161281	162797	1609-1622	1.000000	40.5