primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TACCAATGTCAACCAAGCCA	CGTGCGGTGTAATTCCATTG	20	20	45.0	50.0	57.3	57.8	0.0	0.0	0.838864	1389	3973	2741-2757	1.000000	73.9
trnK-UUU_p2	trnK-UUU	2	ACCAATGTCAACCAAGCCAG	CGTGCGGTGTAATTCCATTG	20	20	50.0	50.0	58.7	57.8	0.0	0.0	0.841379	1389	3973	2740-2756	1.000000	73.9
trnK-UUU_p3	trnK-UUU	3	ATACCAATGTCAACCAAGCCA	CGTGCGGTGTAATTCCATTG	21	20	42.9	50.0	57.8	57.8	0.0	0.0	1.340628	1389	3973	2742-2758	1.000000	69.9
trnK-UUU_p4	trnK-UUU	4	TACCAATGTCAACCAAGCCAG	CGTGCGGTGTAATTCCATTG	21	20	47.6	50.0	58.5	57.8	0.0	0.0	1.658839	1389	3973	2741-2757	1.000000	67.3
trnK-UUU_p5	trnK-UUU	5	CAATGTCAACCAAGCCAGC	CGTGCGGTGTAATTCCATTG	19	20	52.6	50.0	57.5	57.8	0.0	0.0	1.674830	1389	3973	2738-2754	1.000000	67.2
rps16_p1	rps16	1	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGA	20	22	45.0	40.9	57.2	57.1	0.0	18.3	3.633223	4530	5616	1248-1276	1.000000	51.1
rps16_p2	rps16	2	TGGCACGACATAAATCCAGT	TCAATTCCCTATACCACTTTGCA	20	23	45.0	39.1	57.2	57.8	0.0	0.0	3.966011	4530	5616	1253-1281	1.000000	48.4
rps16_p3	rps16	3	TGGCACGACATAAATCCAGT	TCCCTATACCACTTTGCAATGAA	20	23	45.0	39.1	57.2	57.8	0.0	18.3	3.966011	4530	5616	1248-1276	1.000000	48.4
rps16_p4	rps16	4	TGGCACGACATAAATCCAGT	TTCCCTATACCACTTTGCAATGA	20	23	45.0	39.1	57.2	57.8	0.0	18.3	3.966011	4530	5616	1249-1277	1.000000	48.4
rps16_p5	rps16	5	TGGCACGACATAAATCCAGT	CCCTATACCACTTTGCAATGA	20	21	45.0	42.9	57.2	55.6	0.0	18.3	4.140464	4530	5616	1247-1275	1.000000	47.0
psbD_p1	psbD	1	AGTGATATGAGGTGCTCGGA	GTCACGACCAGCTAAAACGA	20	20	50.0	50.0	57.9	58.2	0.0	8.3	0.298614	9459	10520	1142-1142	1.000000	77.8
psbD_p2	psbD	2	TGCTCGGAAATGGTTGAAGT	GTCACGACCAGCTAAAACGA	20	20	45.0	50.0	57.7	58.2	0.0	8.3	0.495713	9459	10520	1130-1130	1.000000	76.2
psbD_p3	psbD	3	AGTGATATGAGGTGCTCGGA	TCACGACCAGCTAAAACGAA	20	20	50.0	45.0	57.9	57.5	0.0	8.3	0.585737	9459	10520	1141-1141	1.000000	75.5
psbD_p4	psbD	4	TGCTCGGAAATGGTTGAAGT	TCACGACCAGCTAAAACGAA	20	20	45.0	45.0	57.7	57.5	0.0	8.3	0.782837	9459	10520	1129-1129	1.000000	73.9
psbD_p5	psbD	5	GTGCTCGGAAATGGTTGAAG	GTCACGACCAGCTAAAACGA	20	20	50.0	50.0	57.4	58.2	0.0	8.3	0.844375	9459	10520	1131-1131	1.000000	73.4
trnG-UCC_p1	trnG-UCC	1	AGGACGCCTTAAGTATATCA	CTCGAATGGCTACCCTTGAC	20	20	40.0	55.0	52.9	58.1	1.0	0.0	5.116478	14384	14406	130-130	1.000000	40.6
trnG-UCC_p2	trnG-UCC	2	AGGACGCCTTAAGTATATCATTTCA	CTCGAATGGCTACCCTTGAC	25	20	36.0	55.0	57.9	58.1	1.0	0.0	5.121424	14384	14406	130-130	1.000000	40.6
trnG-UCC_p3	trnG-UCC	3	AGGACGCCTTAAGTATATCA	CGAATGGCTACCCTTGACAA	20	20	40.0	50.0	52.9	57.9	1.0	0.0	5.165753	14384	14406	128-128	1.000000	40.6
trnG-UCC_p4	trnG-UCC	4	AGGACGCCTTAAGTATATCATTTCA	CGAATGGCTACCCTTGACAA	25	20	36.0	50.0	57.9	57.9	1.0	0.0	5.170699	14384	14406	128-128	1.000000	40.6
trnG-UCC_p5	trnG-UCC	5	AAGGACGCCTTAAGTATATCA	CTCGAATGGCTACCCTTGAC	21	20	38.1	55.0	53.9	58.1	9.1	0.0	5.189951	14384	14406	131-131	1.000000	40.6
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	TTTCCACCGAGCTGAAACAA	GCGTTCAGACCAAAAATGCA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.178301	14407	16401	2648-2690	1.000000	81.4
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	CATCGTTAGCTTGGAAGGCT	GCGTTCAGACCAAAAATGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14407	16401	2929-2971	1.000000	81.3
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	TTTCCACCGAGCTGAAACAA	TGCTTCGTTTAGCGTTCAGA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.188569	14407	16401	2659-2701	1.000000	81.3
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	CATCGTTAGCTTGGAAGGCT	TGCTTCGTTTAGCGTTCAGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.172846	14407	16401	2940-2982	1.000000	81.2
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	CGTTAGCTTGGAAGGCTAGG	GCGTTCAGACCAAAAATGCA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.191415	14407	16401	2926-2968	1.000000	81.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48446	49261	917-924	1.000000	80.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48446	49261	909-916	1.000000	79.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48446	49261	887-894	1.000000	79.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48446	49261	907-914	1.000000	78.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48446	49261	916-923	1.000000	77.8
rbcL-psaI_p1	rbcL-psaI	1	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.195661	58481	59772	1382-1444	1.000000	82.6
rbcL-psaI_p2	rbcL-psaI	2	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.272195	58481	59772	1388-1450	1.000000	82.0
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	58481	59772	1359-1421	1.000000	81.2
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	58481	59772	1364-1426	1.000000	80.8
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	58481	59772	1365-1427	1.000000	80.6
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.9	57.8	8.4	0.0	0.327817	60812	61211	504-553	1.000000	78.3
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	20	20	50.0	55.0	58.1	57.8	8.4	0.0	0.363893	60812	61211	506-555	1.000000	78.0
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.399458	60812	61211	533-582	1.000000	77.7
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.7	57.8	8.4	0.0	0.570031	60812	61211	509-558	1.000000	76.4
ycf4-cemA_p5	ycf4-cemA	5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	20	20	50.0	55.0	58.4	57.8	8.4	0.0	0.598745	60812	61211	513-562	1.000000	76.1
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.9	58.0	0.0	0.0	2.056243	63092	64075	1186-1192	1.000000	64.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.9	57.9	0.0	0.0	2.117200	63092	64075	1185-1191	1.000000	63.5
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	TTCCTCTTTGGCTGATAGGT	20	20	50.0	45.0	57.9	55.8	0.0	0.0	2.229737	63092	64075	1187-1193	1.000000	62.6
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	63092	64075	1121-1127	1.000000	62.6
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	63092	64075	1120-1126	1.000000	62.6
clpP_p1	clpP	1	ATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	20	22	40.0	40.9	55.3	57.7	2.2	0.0	4.974091	70316	70966	758-758	1.000000	40.9
clpP_p2	clpP	2	AATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	21	22	38.1	40.9	56.1	57.7	2.2	0.0	5.164101	70316	70966	759-759	1.000000	40.7
clpP_p3	clpP	3	ATTTACAAGAAATCCGGCCA	GCGGATTCTTTCTTTCTCTTCC	20	22	40.0	45.5	55.3	57.4	2.2	0.0	5.303189	70316	70966	755-755	1.000000	40.7
clpP_p4	clpP	4	TCGCAGAAAACCTGAATTTAGG	ACTGCGGATTCTTTCTTTCTCT	22	22	40.9	40.9	57.0	57.7	14.5	0.0	5.306673	70316	70966	790-790	1.000000	40.7
clpP_p5	clpP	5	AAATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	22	22	36.4	40.9	56.9	57.7	2.2	0.0	5.427617	70316	70966	760-760	1.000000	40.7
psbB_p1	psbB	1	TGACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	21	22	52.4	45.5	58.2	57.8	0.0	0.0	3.490306	71461	72987	1708-1708	1.000000	52.1
psbB_p2	psbB	2	TGACACAGAATCCCCTAGAGG	GAGTCAAAGAAAGGATGGGAGA	21	22	52.4	45.5	58.2	57.4	0.0	0.0	3.807832	71461	72987	1715-1715	1.000000	49.5
psbB_p3	psbB	3	TGACACAGAATCCCCTAGAGG	AGAGTCAAAGAAAGGATGGGAG	21	22	52.4	45.5	58.2	57.4	0.0	0.0	3.814396	71461	72987	1716-1716	1.000000	49.5
psbB_p4	psbB	4	GACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	20	22	55.0	45.5	56.4	57.8	0.0	0.0	3.824055	71461	72987	1707-1707	1.000000	49.4
psbB_p5	psbB	5	TGACACAGAATCCCCTAGAGG	GGAGATGTTTCCCATGTCAA	21	20	52.4	45.0	58.2	55.3	0.0	2.5	3.923997	71461	72987	1698-1698	1.000000	48.6
psbT-psbN_p1	psbT-psbN	1	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.043983	73266	73313	116-116	1.000000	83.1
psbT-psbN_p2	psbT-psbN	2	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.035298	73266	73313	121-121	1.000000	83.0
psbT-psbN_p3	psbT-psbN	3	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026579	73266	73313	208-208	1.000000	81.7
psbT-psbN_p4	psbT-psbN	4	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	73266	73313	203-203	1.000000	81.7
psbT-psbN_p5	psbT-psbN	5	TCTTCTTCCGAGAACCACCT	GTCGCCATCTCCATATCTGG	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.387930	73266	73313	209-209	1.000000	78.8
ndhF_p1	ndhF	1	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAACA	20	21	55.0	42.9	57.8	58.3	0.0	0.0	1.526513	105247	107466	2363-2363	1.000000	68.0
ndhF_p2	ndhF	2	GGAGAGATGGGGGAAGAAGA	GGTTTGGTGAATCGGAAACA	20	20	55.0	45.0	57.8	56.5	0.0	0.0	1.638224	105247	107466	2362-2362	1.000000	67.1
ndhF_p3	ndhF	3	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAAC	20	20	55.0	45.0	57.8	56.5	0.0	0.0	1.638224	105247	107466	2363-2363	1.000000	67.1
ndhF_p4	ndhF	4	AGAAAAGGGGGAGAGATGGG	TGGTTTGGTGAATCGGAAACA	20	21	55.0	42.9	58.4	58.3	0.0	0.0	1.742749	105247	107466	2372-2372	1.000000	66.3
ndhF_p5	ndhF	5	GGAGACGGGGGAATTCCTTA	TGGTTTGGTGAATCGGAAACA	20	21	55.0	42.9	58.5	58.3	23.6	0.0	1.840197	105247	107466	2312-2312	1.000000	65.5
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	20	24	55.0	33.3	58.1	57.3	0.0	0.0	4.844538	107467	108332	977-1022	1.000000	41.7
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107467	108332	949-994	1.000000	41.5
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107467	108332	950-995	1.000000	41.5
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107467	108332	951-996	1.000000	41.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107467	108332	952-997	1.000000	41.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108513	109215	775-812	1.000000	78.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108513	109215	774-811	1.000000	78.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGATCATTTTCCAGCGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	1.4	0.0	0.286165	108513	109215	812-849	1.000000	78.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108513	109215	768-805	1.000000	78.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108513	109215	767-804	1.000000	78.0
ndhG_p1	ndhG	1	ACCATGTTTCGGCTTGTGAT	GCCCGATAGATCTACCCCTT	20	20	45.0	55.0	58.1	58.1	0.0	4.1	0.150320	113390	113920	1392-1407	1.000000	79.6
ndhG_p2	ndhG	2	ACCATGTTTCGGCTTGTGAT	ATGCCCGATAGATCTACCCC	20	20	45.0	55.0	58.1	58.1	0.0	4.1	0.222937	113390	113920	1394-1409	1.000000	79.0
ndhG_p3	ndhG	3	ACCATGTTTCGGCTTGTGAT	TGTGAAGTATGTGTTCGCGT	20	20	45.0	45.0	58.1	57.9	0.0	5.8	0.233308	113390	113920	1414-1429	1.000000	78.9
ndhG_p4	ndhG	4	ACCATGTTTCGGCTTGTGAT	ATTGTGTTGAGTACTGCCCG	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.281890	113390	113920	1287-1302	1.000000	78.6
ndhG_p5	ndhG	5	GCTCTAACCATGTTTCGGCT	GCCCGATAGATCTACCCCTT	20	20	50.0	55.0	58.3	58.1	0.0	4.1	0.320953	113390	113920	1398-1413	1.000000	78.2
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113921	114166	483-495	1.000000	68.7
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113921	114166	484-496	1.000000	68.7
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	20	21	50.0	47.6	58.2	58.5	0.0	1.1	1.718036	113921	114166	484-496	1.000000	67.7
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	20	21	50.0	47.6	58.2	57.4	0.0	1.1	1.854682	113921	114166	485-497	1.000000	66.6
ndhG-ndhI_p5	ndhG-ndhI	5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	20	21	50.0	47.6	58.2	57.2	0.0	1.1	2.071228	113921	114166	483-495	1.000000	64.9