primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TCACAATCCACTGCCTTGTC	TAACCGGGCTAACCTTGGTA	20	20	50.0	50.0	58.1	58.1	0.0	6.3	0.153981	77	436	497-497	1.000000	82.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TCACAATCCACTGCCTTGTC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.178509	77	436	445-445	1.000000	82.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TTCACAATCCACTGCCTTGT	TAACCGGGCTAACCTTGGTA	20	20	45.0	50.0	57.6	58.1	0.0	6.3	0.413507	77	436	498-498	1.000000	80.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TTCACAATCCACTGCCTTGT	TTCCCTCTAGACCTAGCTGC	20	20	45.0	55.0	57.6	57.9	0.0	0.0	0.438035	77	436	446-446	1.000000	80.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TCACAATCCACTGCCTTGTC	ATTAACCGGGCTAACCTTGG	20	20	50.0	50.0	58.1	57.6	0.0	6.3	0.534028	77	436	499-499	1.000000	79.7
psbA_p1	psbA	1	TCACAATCCACTGCCTTGTC	TTCGGTAGAGTCAAGTGGGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.109151	437	1516	1668-1668	0.500000	59.1
psbA_p2	psbA	2	TCCGCCCCTCTACTATGTTT	TTCGGTAGAGTCAAGTGGGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175382	437	1516	1636-1636	0.500000	58.6
psbA_p3	psbA	3	TCACAATCCACTGCCTTGTC	CCGCGAAAGTCTGCTAGAAT	20	20	50.0	50.0	58.1	58.1	0.0	8.2	0.175885	437	1516	1648-1648	0.500000	58.6
psbA_p4	psbA	4	TCACAATCCACTGCCTTGTC	AAGCACGAAAGCGAAGATCT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.189835	437	1516	1943-1943	0.500000	58.5
psbA_p5	psbA	5	TCACAATCCACTGCCTTGTC	AGCACGAAAGCGAAGATCTT	20	20	50.0	45.0	58.1	57.9	0.0	8.0	0.189835	437	1516	1942-1942	0.500000	58.5
psbA-trnQ-UUG_p1	psbA-trnQ-UUG	1	CGGTTTTCAGGGCTGGTTAT	TTGAACCCGGCATATCGAAG	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.217708	1517	2760	1383-1383	0.500000	58.3
psbA-trnQ-UUG_p2	psbA-trnQ-UUG	2	ACCATCCAATGTAAAGGCGG	TTGAACCCGGCATATCGAAG	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.289188	1517	2760	1400-1400	0.500000	57.7
psbA-trnQ-UUG_p3	psbA-trnQ-UUG	3	CCATCCAATGTAAAGGCGGT	TTGAACCCGGCATATCGAAG	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.289188	1517	2760	1399-1399	0.500000	57.7
psbA-trnQ-UUG_p4	psbA-trnQ-UUG	4	AAGCGACCCCATAAGCTTTC	TTGAACCCGGCATATCGAAG	20	20	50.0	50.0	58.2	58.1	3.2	0.0	0.300459	1517	2760	1353-1353	0.500000	57.6
psbA-trnQ-UUG_p5	psbA-trnQ-UUG	5	CGGTTTTCAGGGCTGGTTAT	CCTGTTGCTACAAACGCATC	20	20	50.0	50.0	58.2	57.8	0.0	1.9	0.413123	1517	2760	1447-1447	0.500000	56.7
trnQ-UUG-petN_p1	trnQ-UUG-petN	1	GCTGGTATCACATCTCCGTC	CATTGGTCGAAGAGGCTCAG	20	20	55.0	55.0	57.9	58.4	0.0	18.3	0.491871	4753	4872	280-280	1.000000	76.4
trnQ-UUG-petN_p2	trnQ-UUG-petN	2	GTCCGCTGGTATCACATCTC	CATTGGTCGAAGAGGCTCAG	20	20	55.0	55.0	57.9	58.4	0.0	18.3	0.491871	4753	4872	284-284	1.000000	76.3
trnQ-UUG-petN_p3	trnQ-UUG-petN	3	GGAGATTGTCCGCTGGTATC	CATTGGTCGAAGAGGCTCAG	20	20	55.0	55.0	57.8	58.4	0.0	18.3	0.506679	4753	4872	291-291	1.000000	76.2
trnQ-UUG-petN_p4	trnQ-UUG-petN	4	GCTGGTATCACATCTCCGTC	ATTGGTCGAAGAGGCTCAGA	20	20	55.0	50.0	57.9	58.4	0.0	18.3	0.579892	4753	4872	279-279	1.000000	75.6
trnQ-UUG-petN_p5	trnQ-UUG-petN	5	GTCCGCTGGTATCACATCTC	ATTGGTCGAAGAGGCTCAGA	20	20	55.0	50.0	57.9	58.4	0.0	18.3	0.579892	4753	4872	283-283	1.000000	75.6
trnfM-CAU-rpl32_p1	trnfM-CAU-rpl32	1	TCATCGGTTCAAATCCGGTC	ATGCCAAAGCTTTTAACGCC	20	20	50.0	45.0	58.0	57.9	0.0	9.5	0.097390	16996	18349	1296-1481	1.000000	83.3
trnfM-CAU-rpl32_p2	trnfM-CAU-rpl32	2	CATCGGTTCAAATCCGGTCT	ATGCCAAAGCTTTTAACGCC	20	20	50.0	45.0	58.0	57.9	0.0	9.5	0.103024	16996	18349	1295-1480	1.000000	83.3
trnfM-CAU-rpl32_p3	trnfM-CAU-rpl32	3	TCATCGGTTCAAATCCGGTC	AGCCCTTCCCTTTCCAAATC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.256626	16996	18349	1269-1454	1.000000	82.1
trnfM-CAU-rpl32_p4	trnfM-CAU-rpl32	4	TCATCGGTTCAAATCCGGTC	GAGCCCTTCCCTTTCCAAAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.256626	16996	18349	1270-1455	1.000000	82.1
trnfM-CAU-rpl32_p5	trnfM-CAU-rpl32	5	TCATCGGTTCAAATCCGGTC	GCCCTTCCCTTTCCAAATCT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.256626	16996	18349	1268-1453	1.000000	82.1
rpoC2-rps2_p1	rpoC2-rps2	1	TCTTCGAGGGGGAAATGAGA	AGGTGCCATTTTCGGATTCC	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.773369	41034	41282	467-467	1.000000	74.3
rpoC2-rps2_p2	rpoC2-rps2	2	TTCGAGGGGGAAATGAGAGA	AGGTGCCATTTTCGGATTCC	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.773369	41034	41282	465-465	1.000000	74.3
rpoC2-rps2_p3	rpoC2-rps2	3	TCTTCGAGGGGGAAATGAGA	CATGGCCAAAATGAACTCCC	20	20	50.0	50.0	57.8	57.3	0.0	11.0	0.926170	41034	41282	435-435	1.000000	73.1
rpoC2-rps2_p4	rpoC2-rps2	4	TTCGAGGGGGAAATGAGAGA	CATGGCCAAAATGAACTCCC	20	20	50.0	50.0	57.8	57.3	0.0	11.0	0.926170	41034	41282	433-433	1.000000	73.1
rpoC2-rps2_p5	rpoC2-rps2	5	TCGAGGGGGAAATGAGAGAT	AGGTGCCATTTTCGGATTCC	20	20	50.0	50.0	57.5	58.5	0.0	0.0	1.004542	41034	41282	464-464	1.000000	72.5
atpI-atpH_p1	atpI-atpH	1	TTTTGCAACTTTAGCTGCGG	ATAACGGAAGCGGCAGAAAT	20	20	45.0	45.0	57.9	58.0	0.0	1.4	0.171127	42952	45223	2359-2359	1.000000	78.8
atpI-atpH_p2	atpI-atpH	2	TTTTGCAACTTTAGCTGCGG	TACCTTGACCAACTCCAGGT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.245019	42952	45223	2415-2415	1.000000	78.2
atpI-atpH_p3	atpI-atpH	3	TTTTGCAACTTTAGCTGCGG	AGCCAATCCAGCAGCAATAA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.281186	42952	45223	2375-2375	1.000000	77.9
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	42952	45223	2408-2408	1.000000	77.8
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACTTTAGCTGCGG	AATAACGGAAGCGGCAGAAA	20	20	45.0	45.0	57.9	58.2	0.0	1.4	0.316666	42952	45223	2360-2360	1.000000	77.6
ycf4_p1	ycf4	1	AATGGGGTCATACGAAGGAT	TAAGCCTGCTTTAGCTCGTG	20	20	45.0	50.0	56.0	58.0	0.0	10.0	2.033793	60194	60835	852-852	1.000000	63.8
ycf4_p2	ycf4	2	AATGGGGTCATACGAAGGAT	GTTCGGACGAAACCTCAGTT	20	20	45.0	50.0	56.0	58.1	0.0	8.9	2.161677	60194	60835	833-833	1.000000	62.8
ycf4_p3	ycf4	3	TGGGGTCATACGAAGGATATG	TAAGCCTGCTTTAGCTCGTG	21	20	47.6	50.0	56.7	58.0	0.0	10.0	2.338034	60194	60835	850-850	1.000000	61.4
ycf4_p4	ycf4	4	GGGGTCATACGAAGGATATGT	TAAGCCTGCTTTAGCTCGTG	21	20	47.6	50.0	56.7	58.0	0.0	10.0	2.340224	60194	60835	849-849	1.000000	61.4
ycf4_p5	ycf4	5	AATGGGGTCATACGAAGGAT	TCCCCTCCTGCTGAGAAAAT	20	20	45.0	50.0	56.0	58.3	0.0	5.5	2.349881	60194	60835	765-765	1.000000	61.3
petA-psbJ_p1	petA-psbJ	1	GGCAGCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.3	57.6	16.6	4.1	0.651376	63642	64675	1138-1145	1.000000	75.3
petA-psbJ_p2	petA-psbJ	2	GCAGCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.6	57.6	16.6	4.1	0.802172	63642	64675	1137-1144	1.000000	74.1
petA-psbJ_p3	petA-psbJ	3	TTGGCAGCTGTTATTTTGGC	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.5	57.6	16.6	4.1	0.818956	63642	64675	1140-1147	1.000000	73.9
petA-psbJ_p4	petA-psbJ	4	TGGCAGCTGTTATTTTGGCA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.7	57.6	16.6	4.1	1.028841	63642	64675	1139-1146	1.000000	72.3
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.2	57.6	0.0	4.1	1.119919	63642	64675	1171-1178	1.000000	71.5
ndhJ-petD_p1	ndhJ-petD	1	TTTCATGCCGGAAAGTTGGA	CCCATTTCGTCGTCCAGTAG	20	20	45.0	55.0	58.0	58.1	5.0	0.0	0.089136	68631	69244	817-817	1.000000	79.4
ndhJ-petD_p2	ndhJ-petD	2	TTTCATGCCGGAAAGTTGGA	TTGGTTGGGTATTGGTGCAA	20	20	45.0	45.0	58.0	57.9	5.0	0.0	0.099811	68631	69244	760-760	1.000000	79.3
ndhJ-petD_p3	ndhJ-petD	3	TTTCATGCCGGAAAGTTGGA	TTTGGTTGGGTATTGGTGCA	20	20	45.0	45.0	58.0	57.9	5.0	0.0	0.099811	68631	69244	761-761	1.000000	79.3
ndhJ-petD_p4	ndhJ-petD	4	TTTCATGCCGGAAAGTTGGA	CAGTAGCGACAACCGTCTTT	20	20	45.0	50.0	58.0	58.2	5.0	0.0	0.232137	68631	69244	803-803	1.000000	78.2
ndhJ-petD_p5	ndhJ-petD	5	CGGATTTTCATGCCGGAAAG	CCCATTTCGTCGTCCAGTAG	20	20	50.0	55.0	57.8	58.1	5.0	0.0	0.270736	68631	69244	822-822	1.000000	77.9
trnM-CAU-trnfM-CAU_p1	trnM-CAU-trnfM-CAU	1	GGATGGGTCCCATTTGGTTT	TCATCGGTTCAAATCCGGTC	20	20	50.0	50.0	58.0	58.0	24.8	0.0	0.067333	74939	76219	1418-1418	0.500000	59.5
trnM-CAU-trnfM-CAU_p2	trnM-CAU-trnfM-CAU	2	GGATGGGTCCCATTTGGTTT	CATCGGTTCAAATCCGGTCT	20	20	50.0	50.0	58.0	58.0	24.8	0.0	0.072966	74939	76219	1417-1417	0.500000	59.4
trnM-CAU-trnfM-CAU_p3	trnM-CAU-trnfM-CAU	3	GGATGGGTCCCATTTGGTTT	TGGTAGCTCGTAAGGCTCAT	20	20	50.0	50.0	58.0	58.2	24.8	0.0	0.272129	74939	76219	1448-1448	0.500000	57.8
trnM-CAU-trnfM-CAU_p4	trnM-CAU-trnfM-CAU	4	GGATGGGTCCCATTTGGTTT	TCTTCTCTTAAGGGCGGAGT	20	20	50.0	50.0	58.0	57.8	24.8	1.9	0.282096	74939	76219	1477-1477	0.500000	57.7
trnM-CAU-trnfM-CAU_p5	trnM-CAU-trnfM-CAU	5	GGATGGGTCCCATTTGGTTT	GTCATCGGTTCAAATCCGGT	20	20	50.0	50.0	58.0	58.3	24.8	0.0	0.314628	74939	76219	1419-1419	0.500000	57.5
rps14_p1	rps14	1	GCGCCTCTCTACCATTCTG	AGAGTCCGTTTTGTGATTTTGC	19	22	57.9	40.9	57.7	58.0	0.0	0.0	3.316011	76463	76765	524-524	1.000000	53.6
rps14_p2	rps14	2	GCGCCTCTCTACCATTCTG	GAGTCCGTTTTGTGATTTTGCT	19	22	57.9	40.9	57.7	58.0	0.0	0.0	3.316011	76463	76765	523-523	1.000000	53.6
rps14_p3	rps14	3	GCGCCTCTCTACCATTCTG	AGTCCGTTTTGTGATTTTGCT	19	21	57.9	38.1	57.7	56.9	0.0	0.0	3.356440	76463	76765	522-522	1.000000	53.3
rps14_p4	rps14	4	CGCCTCTCTACCATTCTGTAC	AGAGTCCGTTTTGTGATTTTGC	21	22	52.4	40.9	57.6	58.0	0.0	0.0	3.392256	76463	76765	523-523	1.000000	53.0
rps14_p5	rps14	5	CGCCTCTCTACCATTCTGTAC	GAGTCCGTTTTGTGATTTTGCT	21	22	52.4	40.9	57.6	58.0	0.0	0.0	3.392256	76463	76765	522-522	1.000000	53.0
clpP-trnL-UAA_p1	clpP-trnL-UAA	1	TCAGGATGAGGACGAGAAGA	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.878339	84844	87412	2662-2662	1.000000	73.0
clpP-trnL-UAA_p2	clpP-trnL-UAA	2	TCAGGATGAGGACGAGAAGA	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.2	58.2	0.0	10.2	1.048714	84844	87412	2668-2668	1.000000	71.6
clpP-trnL-UAA_p3	clpP-trnL-UAA	3	TCAGGATGAGGACGAGAAGA	GTACGTTTCCCTACCAAGGC	20	20	50.0	55.0	57.2	58.3	0.0	0.0	1.078405	84844	87412	2700-2700	1.000000	71.4
clpP-trnL-UAA_p4	clpP-trnL-UAA	4	TCAGGATGAGGACGAGAAGAA	TAGCGTCTACCAATTTCGCC	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.156802	84844	87412	2662-2662	1.000000	70.8
clpP-trnL-UAA_p5	clpP-trnL-UAA	5	TCAGGATGAGGACGAGAAGA	TACCAATTTCGCCATACCCC	20	20	50.0	50.0	57.2	57.6	0.0	0.0	1.160612	84844	87412	2655-2655	1.000000	70.7
rpl16-rps3_p1	rpl16-rps3	1	AAACCAACGAGTCACACACT	GGCTTCCCCTACAAACCATT	20	20	45.0	50.0	57.6	57.8	0.0	0.0	0.628306	94606	94781	311-312	1.000000	75.2
rpl16-rps3_p2	rpl16-rps3	2	GCAAAGCGATGAGTGGGTTA	GGCTTCCCCTACAAACCATT	20	20	50.0	50.0	58.6	57.8	0.0	0.0	0.782732	94606	94781	392-393	1.000000	73.9
rpl16-rps3_p3	rpl16-rps3	3	AAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	20	20	45.0	50.0	57.6	57.6	0.0	0.0	0.799841	94606	94781	305-306	1.000000	73.9
rpl16-rps3_p4	rpl16-rps3	4	GCAAAGCGATGAGTGGGTTA	CCCTACAAACCATTCGAGCT	20	20	50.0	50.0	58.6	57.6	0.0	0.0	0.954267	94606	94781	386-387	1.000000	72.6
rpl16-rps3_p5	rpl16-rps3	5	AAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	20	20	45.0	55.0	57.6	58.6	0.0	0.0	1.017063	94606	94781	306-307	1.000000	72.1
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	AGGTTCAGTCTCCGATTCCT	TTTCGTTCTATACCGGCACC	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.538092	140832	140951	223-223	1.000000	76.8
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	ACTCAAATGATCCACGGCTG	TTTCGTTCTATACCGGCACC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.555650	140832	140951	290-290	1.000000	76.4
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	CACTCAAATGATCCACGGCT	TTTCGTTCTATACCGGCACC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.555650	140832	140951	291-291	1.000000	76.4
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	ACGGCTGCCAATATATCTCG	TTTCGTTCTATACCGGCACC	20	20	50.0	50.0	57.6	57.7	20.3	0.0	0.668692	140832	140951	277-277	1.000000	75.5
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	CTGCCAATATATCTCGCGGT	TTTCGTTCTATACCGGCACC	20	20	50.0	50.0	57.6	57.7	6.1	0.0	0.668692	140832	140951	273-273	1.000000	75.5
psaC_p1	psaC	1	GGGCCATAAAACCCATACGT	TCTGGAAACTCATCGATTCGT	20	21	50.0	42.9	57.9	57.1	4.4	5.9	1.930266	144525	144770	447-447	1.000000	64.7
psaC_p2	psaC	2	GGGCCATAAAACCCATACGT	TGGAAACTCATCGATTCGTCT	20	21	50.0	42.9	57.9	57.1	4.4	5.9	1.930266	144525	144770	445-445	1.000000	64.7
psaC_p3	psaC	3	GGGCCATAAAACCCATACGT	CTGGAAACTCATCGATTCGTC	20	21	50.0	47.6	57.9	56.8	4.4	5.9	2.244663	144525	144770	446-446	1.000000	62.2
psaC_p4	psaC	4	GGGCCATAAAACCCATACGT	CTCTGGAAACTCATCGATTCGT	20	22	50.0	45.5	57.9	58.2	4.4	5.9	2.286307	144525	144770	448-448	1.000000	61.9
psaC_p5	psaC	5	GGGCCATAAAACCCATACGT	CTGGAAACTCATCGATTCGTCT	20	22	50.0	45.5	57.9	58.2	4.4	5.9	2.286307	144525	144770	446-446	1.000000	61.9