primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gat)_p1	rns-trnI(gat)	1	GTAACCGTACTGGAAGGTGC	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	58.3	58.0	0.0	17.4	0.292726	15892	15973	217-241	1.000000	83.9
rns-trnI(gat)_p2	rns-trnI(gat)	2	AGGTAACCGTACTGGAAGGT	TACCAGCTGAGCTATACCCC	20	20	50.0	55.0	57.7	58.0	10.2	17.4	0.326842	15892	15973	219-243	1.000000	83.6
rns-trnI(gat)_p3	rns-trnI(gat)	3	AGTGACTAGGGTGAAGTCGT	TACCAGCTGAGCTATACCCC	20	20	50.0	55.0	57.7	58.0	0.0	17.4	0.300714	15892	15973	243-267	1.000000	83.2
rns-trnI(gat)_p4	rns-trnI(gat)	4	GGTAACCGTACTGGAAGGTG	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	57.6	58.0	0.0	17.4	0.386295	15892	15973	218-242	1.000000	83.1
rns-trnI(gat)_p5	rns-trnI(gat)	5	GGAGGAGGATGCCTAAGGTA	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	57.6	58.0	0.0	17.4	0.411589	15892	15973	268-292	1.000000	81.8
rnl-rrn5_p1	rnl-rrn5	1	GATGTGCTAACAGACCGAGG	GTGGTTCCGCTGAATACACT	20	20	55.0	50.0	58.1	57.9	0.0	16.1	0.166867	19036	19086	105-106	1.000000	85.5
rnl-rrn5_p2	rnl-rrn5	2	GATGTGCTAACAGACCGAGG	AGTGTGGTTCCGCTGAATAC	20	20	55.0	50.0	58.1	57.9	0.0	4.0	0.166867	19036	19086	108-109	1.000000	85.3
rnl-rrn5_p3	rnl-rrn5	3	GATGTGCTAACAGACCGAGG	CAGTGTGGTTCCGCTGAATA	20	20	55.0	50.0	58.1	57.9	0.0	4.0	0.165411	19036	19086	109-110	1.000000	85.2
rnl-rrn5_p4	rnl-rrn5	4	GATGTGCTAACAGACCGAGG	TGTGGTTCCGCTGAATACAC	20	20	55.0	50.0	58.1	58.2	0.0	16.3	0.268771	19036	19086	106-107	1.000000	84.6
rnl-rrn5_p5	rnl-rrn5	5	GATGTGCTAACAGACCGAGG	TCGAGATGGATCAGTGTGGT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.219472	19036	19086	120-121	1.000000	84.2
rrn5-rps10_p1	rrn5-rps10	1	GCTAACAGACCGAGGACTTG	TTACCCTGTTGCGATCGAAG	20	20	55.0	50.0	58.0	58.0	0.0	21.6	0.006913	19215	19918	1181-1328	0.400000	67.9
rrn5-rps10_p2	rrn5-rps10	2	ATCTCCCCCAAGAGTTCACA	TTACCCTGTTGCGATCGAAG	20	20	50.0	50.0	58.0	58.0	0.0	21.6	0.049375	19215	19918	1597-1744	0.400000	67.6
rrn5-rps10_p3	rrn5-rps10	3	TCTCCCCCAAGAGTTCACAT	TTACCCTGTTGCGATCGAAG	20	20	50.0	50.0	58.0	58.0	0.0	21.6	0.049375	19215	19918	1596-1743	0.400000	67.6
rrn5-rps10_p4	rrn5-rps10	4	TTCGGTCCATATCCGGTGTA	TTACCCTGTTGCGATCGAAG	20	20	50.0	50.0	57.9	58.0	0.0	21.6	0.074888	19215	19918	1452-1599	0.400000	67.4
rrn5-rps10_p5	rrn5-rps10	5	GATGTGCTAACAGACCGAGG	TTACCCTGTTGCGATCGAAG	20	20	55.0	50.0	58.1	58.0	0.0	21.6	0.076688	19215	19918	1186-1333	0.400000	67.4
trnC(gca)-psbY_p1	trnC(gca)-psbY	1	CCTTACCACTTGGCCATACC	ATAATGCCCATGATGCTGCA	20	20	55.0	45.0	57.9	58.0	6.1	4.1	0.056719	53778	54526	555-2838	1.000000	91.5
trnC(gca)-psbY_p2	trnC(gca)-psbY	2	CCTTACCACTTGGCCATACC	GGTGATGAAGCAATTTGGCC	20	20	55.0	50.0	57.9	58.0	6.1	0.0	0.067118	53778	54526	943-1613	0.600000	75.5
trnC(gca)-psbY_p3	trnC(gca)-psbY	3	CCTTACCACTTGGCCATACC	ACACGTGGTGATGAAGCAAT	20	20	55.0	45.0	57.9	58.1	6.1	21.6	0.156563	53778	54526	1223-1619	0.400000	66.7
trnC(gca)-psbY_p4	trnC(gca)-psbY	4	CCTTACCACTTGGCCATACC	ACAGGCTGTTTCACATCGTT	20	20	55.0	45.0	57.9	58.0	6.1	15.9	0.085464	53778	54526	1263-1263	0.200000	47.3
trnC(gca)-psbY_p5	trnC(gca)-psbY	5	CCTTACCACTTGGCCATACC	TCTGTTGGACAGGCTGTTTC	20	20	55.0	50.0	57.9	58.0	6.1	23.0	0.085464	53778	54526	1271-1271	0.200000	47.3
trnR(acg)-trnQ(ttg)_p1	trnR(acg)-trnQ(ttg)	1	TGGACTAGAGCACGTGGTTA	ATGGCAGGATTCGAACCTAC	20	20	50.0	50.0	58.1	57.4	22.8	15.9	0.708286	57039	57077	164-166	1.000000	82.6
trnR(acg)-trnQ(ttg)_p2	trnR(acg)-trnQ(ttg)	2	GGGGTTCGAATCCCTTCTTG	ATGGCAGGATTCGAACCTAC	20	20	55.0	50.0	58.2	57.4	35.1	15.9	0.870627	57039	57077	129-131	1.000000	81.7
trnR(acg)-trnQ(ttg)_p3	trnR(acg)-trnQ(ttg)	3	GGACTAGAGCACGTGGTTAC	ATGGCAGGATTCGAACCTAC	20	20	55.0	50.0	57.7	57.4	22.8	15.9	0.896984	57039	57077	163-165	1.000000	81.1
trnR(acg)-trnQ(ttg)_p4	trnR(acg)-trnQ(ttg)	4	TGGACTAGAGCACGTGGTTA	GATGGCAGGATTCGAACCTA	20	20	50.0	50.0	58.1	57.1	22.8	15.9	0.996910	57039	57077	165-167	1.000000	80.2
trnR(acg)-trnQ(ttg)_p5	trnR(acg)-trnQ(ttg)	5	TGGACTAGAGCACGTGGTTA	GGCAGGATTCGAACCTACG	20	19	50.0	57.9	58.1	58.0	22.8	15.9	1.112802	57039	57077	162-164	1.000000	79.4
rps16-rrn5_p1	rps16-rrn5	1	AAACGAATTGGCCGAAAACG	GCTAACAGACCGAGGACTTG	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.241125	59161	59767	1017-1170	0.400000	63.9
rps16-rrn5_p2	rps16-rrn5	2	AGAAGCACGTCGAAGAAAGG	GCTAACAGACCGAGGACTTG	20	20	50.0	55.0	58.2	58.0	5.1	0.0	0.217806	59161	59767	1464-1464	0.200000	46.3
rps16-rrn5_p3	rps16-rrn5	3	CTCAACCAACGGAGACTGTT	GCTAACAGACCGAGGACTTG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.242824	59161	59767	854-854	0.200000	46.1
rps16-rrn5_p4	rps16-rrn5	4	AGAAGCACGTCGAAGAAAGG	ATCTCCCCCAAGAGTTCACA	20	20	50.0	50.0	58.2	58.0	5.1	0.0	0.260267	59161	59767	1880-1880	0.200000	45.9
rps16-rrn5_p5	rps16-rrn5	5	AGAAGCACGTCGAAGAAAGG	TCTCCCCCAAGAGTTCACAT	20	20	50.0	50.0	58.2	58.0	5.1	0.0	0.260267	59161	59767	1879-1879	0.200000	45.9
rrn5-rnl_p1	rrn5-rnl	1	GTGGTTCCGCTGAATACACT	GATGTGCTAACAGACCGAGG	20	20	50.0	55.0	57.9	58.1	16.1	0.0	0.166867	59896	59946	105-106	1.000000	84.7
rrn5-rnl_p2	rrn5-rnl	2	CAGTGTGGTTCCGCTGAATA	GATGTGCTAACAGACCGAGG	20	20	50.0	55.0	57.9	58.1	4.0	0.0	0.165411	59896	59946	109-110	1.000000	84.5
rrn5-rnl_p3	rrn5-rnl	3	AGTGTGGTTCCGCTGAATAC	GATGTGCTAACAGACCGAGG	20	20	50.0	55.0	57.9	58.1	4.0	0.0	0.166867	59896	59946	108-109	1.000000	84.5
rrn5-rnl_p4	rrn5-rnl	4	TGTGGTTCCGCTGAATACAC	GATGTGCTAACAGACCGAGG	20	20	50.0	55.0	58.2	58.1	16.3	0.0	0.268771	59896	59946	106-107	1.000000	83.8
rrn5-rnl_p5	rrn5-rnl	5	TCGAGATGGATCAGTGTGGT	GATGTGCTAACAGACCGAGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.219472	59896	59946	120-121	1.000000	83.5
trnI(gat)-rns_p1	trnI(gat)-rns	1	TACCAGCTGAGCTATACCCC	GTAACCGTACTGGAAGGTGC	20	20	55.0	55.0	58.0	58.3	17.4	0.0	0.292726	63009	63090	217-241	1.000000	84.2
trnI(gat)-rns_p2	trnI(gat)-rns	2	TACCAGCTGAGCTATACCCC	AGGTAACCGTACTGGAAGGT	20	20	55.0	50.0	58.0	57.7	17.4	10.2	0.326842	63009	63090	219-243	1.000000	83.9
trnI(gat)-rns_p3	trnI(gat)-rns	3	TACCAGCTGAGCTATACCCC	AGTGACTAGGGTGAAGTCGT	20	20	55.0	50.0	58.0	57.7	17.4	0.0	0.300714	63009	63090	243-267	1.000000	83.5
trnI(gat)-rns_p4	trnI(gat)-rns	4	TACCAGCTGAGCTATACCCC	GGTAACCGTACTGGAAGGTG	20	20	55.0	55.0	58.0	57.6	17.4	0.0	0.386295	63009	63090	218-242	1.000000	83.5
trnI(gat)-rns_p5	trnI(gat)-rns	5	TACCAGCTGAGCTATACCCC	GGAGGAGGATGCCTAAGGTA	20	20	55.0	55.0	58.0	57.6	17.4	0.0	0.411589	63009	63090	268-292	1.000000	82.1
psbD-psbK_p1	psbD-psbK	1	AAACCTGACCAACCAACGAA	ACGTATGCTTCTGGTAAACGA	20	21	45.0	42.9	57.9	57.4	0.0	0.0	1.712799	73588	73918	459-474	1.000000	78.3
psbD-psbK_p2	psbD-psbK	2	GCCACGTTCTTGGTTTTGTC	ACGTATGCTTCTGGTAAACGA	20	21	50.0	42.9	58.2	57.4	0.0	0.0	1.745903	73588	73918	394-409	1.000000	78.0
psbD-psbK_p3	psbD-psbK	3	AACCTGACCAACCAACGAAT	ACGTATGCTTCTGGTAAACGA	20	21	45.0	42.9	57.6	57.4	0.0	0.0	1.939345	73588	73918	458-473	1.000000	76.5
psbD-psbK_p4	psbD-psbK	4	CCACGTTCTTGGTTTTGTCC	ACGTATGCTTCTGGTAAACGA	20	21	50.0	42.9	57.5	57.4	0.0	0.0	2.066290	73588	73918	393-408	1.000000	75.5
psbD-psbK_p5	psbD-psbK	5	AGCCACGTTCTTGGTTTTGT	ACGTATGCTTCTGGTAAACGA	20	21	45.0	42.9	58.5	57.4	0.0	0.0	2.110973	73588	73918	395-410	1.000000	75.1
trnT(tgt)-trnR(tct)_p1	trnT(tgt)-trnR(tct)	1	AACCCCCAACCTACGGATTA	TTCGATTCCTACTGGGCCTA	20	20	50.0	50.0	58.0	57.5	0.0	4.5	0.495083	74526	74753	288-382	1.000000	88.0
trnT(tgt)-trnR(tct)_p2	trnT(tgt)-trnR(tct)	2	ATTTGAACCCCCAACCTACG	TTCGATTCCTACTGGGCCTA	20	20	50.0	50.0	57.8	57.5	0.0	4.5	0.674486	74526	74753	293-387	1.000000	86.6
trnT(tgt)-trnR(tct)_p3	trnT(tgt)-trnR(tct)	3	ACAAAACCGTTGCTCTACCA	TTCGATTCCTACTGGGCCTA	20	20	45.0	50.0	57.7	57.5	0.0	4.5	0.800111	74526	74753	269-363	1.000000	85.6
trnT(tgt)-trnR(tct)_p4	trnT(tgt)-trnR(tct)	4	ACCGTTGCTCTACCATTGAG	TTCGATTCCTACTGGGCCTA	20	20	50.0	50.0	57.6	57.5	0.0	4.5	0.851456	74526	74753	264-358	1.000000	85.2
trnT(tgt)-trnR(tct)_p5	trnT(tgt)-trnR(tct)	5	AACCCCCAACCTACGGATTA	GTTCGATTCCTACTGGGCCT	20	20	50.0	55.0	58.0	59.2	0.0	4.5	1.198981	74526	74753	289-383	1.000000	82.4
trnM(cat)-trnR(ccg)_p1	trnM(cat)-trnR(ccg)	1	ACCAGTGATGTTCCAGAGGA	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.074150	76033	76486	525-542	0.800000	82.9
trnM(cat)-trnR(ccg)_p2	trnM(cat)-trnR(ccg)	2	ACCAGTGATGTTCCAGAGGA	AGAGAAATGGGGTGGTGGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.083774	76033	76486	699-717	0.800000	82.0
trnM(cat)-trnR(ccg)_p3	trnM(cat)-trnR(ccg)	3	ACCAGTGATGTTCCAGAGGA	GTGCGGATATTTACCCACGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085996	76033	76486	790-808	0.600000	73.3
trnM(cat)-trnR(ccg)_p4	trnM(cat)-trnR(ccg)	4	ACCAGTGATGTTCCAGAGGA	TGGTGCATGTCACTTAGGTG	20	20	50.0	50.0	58.0	57.8	0.0	16.3	0.213505	76033	76486	987-987	0.200000	46.3
trnM(cat)-trnR(ccg)_p5	trnM(cat)-trnR(ccg)	5	ACCAGTGATGTTCCAGAGGA	TGTGGTGCATGTCACTTAGG	20	20	50.0	50.0	58.0	57.8	0.0	16.3	0.213505	76033	76486	989-989	0.200000	46.3
trnF(gaa)-ycf90_p1	trnF(gaa)-ycf90	1	GATCCTCGTGTCACCAGTTC	GACCGCGTCCAAAGAATAGT	20	20	55.0	50.0	58.0	58.0	0.0	4.9	0.008301	97902	98264	600-1030	0.800000	82.3
trnF(gaa)-ycf90_p2	trnF(gaa)-ycf90	2	TCAGTTGGTAGAGCAGTGGA	GACCGCGTCCAAAGAATAGT	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.015652	97902	98264	625-1055	0.800000	82.2
trnF(gaa)-ycf90_p3	trnF(gaa)-ycf90	3	ATTCCTGGGAACGATGATGC	GACCGCGTCCAAAGAATAGT	20	20	50.0	50.0	58.0	58.0	4.0	4.9	0.033098	97902	98264	871-871	0.200000	47.7
trnF(gaa)-ycf90_p4	trnF(gaa)-ycf90	4	GCACAGGAAGCAAAACGATC	GACCGCGTCCAAAGAATAGT	20	20	50.0	50.0	58.0	58.0	0.0	4.9	0.036867	97902	98264	1228-1228	0.200000	47.7
trnF(gaa)-ycf90_p5	trnF(gaa)-ycf90	5	GATCCTCGTGTCACCAGTTC	ATGGCAGGATGAGAAGCAAG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.056563	97902	98264	557-557	0.200000	47.5
trnS(gct)-trnD(gtc)_p1	trnS(gct)-trnD(gtc)	1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.239769	105191	105271	213-226	1.000000	90.1
trnS(gct)-trnD(gtc)_p2	trnS(gct)-trnD(gtc)	2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.239769	105191	105271	214-227	1.000000	90.1
trnS(gct)-trnD(gtc)_p3	trnS(gct)-trnD(gtc)	3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.244871	105191	105271	215-228	1.000000	90.0
trnS(gct)-trnD(gtc)_p4	trnS(gct)-trnD(gtc)	4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.244871	105191	105271	216-229	1.000000	90.0
trnS(gct)-trnD(gtc)_p5	trnS(gct)-trnD(gtc)	5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.309286	105191	105271	218-231	1.000000	89.5
secG-ycf45_p1	secG-ycf45	1	GTCTGGAAACCCCGAAGAAG	GGCACGAACTATTGCAGAGA	20	20	55.0	50.0	58.2	58.0	0.0	4.8	0.200461	110413	110718	1751-1751	0.200000	46.4
secG-ycf45_p2	secG-ycf45	2	GTCTGGAAACCCCGAAGAAG	ATGTTGCGACATCAGCAGAT	20	20	55.0	45.0	58.2	58.0	0.0	14.2	0.224420	110413	110718	1509-1509	0.200000	46.2
secG-ycf45_p3	secG-ycf45	3	GTCTGGAAACCCCGAAGAAG	AATGTTGCGACATCAGCAGA	20	20	55.0	45.0	58.2	58.2	0.0	20.0	0.378081	110413	110718	1510-1510	0.200000	45.0
secG-ycf45_p4	secG-ycf45	4	GTCTGGAAACCCCGAAGAAG	TGTTGCGACATCAGCAGATT	20	20	55.0	45.0	58.2	58.2	0.0	1.5	0.378081	110413	110718	1508-1508	0.200000	45.0
secG-ycf45_p5	secG-ycf45	5	ATTGTAACAACACGTGCTGC	GGCACGAACTATTGCAGAGA	20	20	45.0	50.0	57.6	58.0	18.5	4.8	0.409541	110413	110718	1453-1453	0.200000	44.7
sufB_p1	sufB	1	TCACCACCAGAAAAGCCTTC	TCTGTCGGTTGATCGTTTGT	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.538120	120667	122127	2175-2175	0.200000	43.7
sufB_p2	sufB	2	AGGCGACATGTTCACAAGTT	TCTGTCGGTTGATCGTTTGT	20	20	45.0	45.0	58.0	57.5	0.0	0.0	0.552058	120667	122127	2131-2131	0.200000	43.6
sufB_p3	sufB	3	GGATGTCCGGCTAAAACCTT	TCTGTCGGTTGATCGTTTGT	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.643654	120667	122127	1878-1878	0.200000	42.9
sufB_p4	sufB	4	CACCACCAGAAAAGCCTTCA	TCTGTCGGTTGATCGTTTGT	20	20	50.0	45.0	58.3	57.5	0.0	0.0	0.828180	120667	122127	2174-2174	0.200000	41.4
sufB_p5	sufB	5	TCACCACCAGAAAAGCCTTC	AGTTCTGTCGGTTGATCGTT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.833023	120667	122127	2178-2178	0.200000	41.3