primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	rps7	1	AATCGGATGTACGGTTTGGG	TGGAAGACCCCAGTAAGGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.160332	1029	1496	967-1023	0.666667	69.0
rps7_p2	rps7	2	AATCGGATGTACGGTTTGGG	TTGGAAGACCCCAGTAAGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.160332	1029	1496	968-1024	0.666667	69.0
rps7_p3	rps7	3	AGGGGGACATTAGATGCTGT	TGGAAGACCCCAGTAAGGTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.228789	1029	1496	1461-1520	0.666667	67.8
rps7_p4	rps7	4	AGGGGGACATTAGATGCTGT	TTGGAAGACCCCAGTAAGGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.228789	1029	1496	1462-1521	0.666667	67.8
rps7_p5	rps7	5	CAAGGGGACTAAAGCATGCT	TGGAAGACCCCAGTAAGGTT	20	20	50.0	50.0	57.9	57.9	16.3	0.0	0.262440	1029	1496	1309-1365	0.666667	67.7
ycf66-petN_p1	ycf66-petN	1	ATCGACTTGGATTTCGGGTG	ATAAAACAAACGGCGGCATG	20	20	50.0	45.0	58.0	58.0	0.0	13.2	0.034745	5347	5430	997-1005	1.000000	83.6
ycf66-petN_p2	ycf66-petN	2	ATCGACTTGGATTTCGGGTG	AGGATCAAACCGTCATGCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	5347	5430	871-876	1.000000	83.6
ycf66-petN_p3	ycf66-petN	3	ATCGACTTGGATTTCGGGTG	GGATCAAACCGTCATGCTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054014	5347	5430	870-875	1.000000	83.6
ycf66-petN_p4	ycf66-petN	4	ATCGACTTGGATTTCGGGTG	TCGTATGGGGAAGAAGTGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.077191	5347	5430	536-538	1.000000	83.3
ycf66-petN_p5	ycf66-petN	5	ATCGACTTGGATTTCGGGTG	GGGTGTCGCTTCATTTTTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060429	5347	5430	931-934	0.666667	68.8
trnR-UCU-trnG-GCC_p1	trnR-UCU-trnG-GCC	1	GGATTCGAACCTATACTGGAGG	TTCCAAGCTAATGATGCGGG	22	20	50.0	50.0	57.5	58.3	0.0	0.0	2.843583	21916	21994	179-179	1.000000	60.4
trnR-UCU-trnG-GCC_p2	trnR-UCU-trnG-GCC	2	GGATTCGAACCTATACTGGAGG	GCTAATGATGCGGGTTCGAT	22	20	50.0	50.0	57.5	58.5	0.0	0.0	3.009796	21916	21994	173-173	1.000000	59.2
trnR-UCU-trnG-GCC_p3	trnR-UCU-trnG-GCC	3	GGATTCGAACCTATACTGGAGG	CCAAGCTAATGATGCGGGTT	22	20	50.0	50.0	57.5	58.6	0.0	0.0	3.130133	21916	21994	177-177	1.000000	58.1
trnR-UCU-trnG-GCC_p4	trnR-UCU-trnG-GCC	4	GGATTCGAACCTATACTGGAGG	AATGATGCGGGTTCGATTCC	22	20	50.0	50.0	57.5	58.7	0.0	0.0	3.213724	21916	21994	170-170	1.000000	57.6
trnR-UCU-trnG-GCC_p5	trnR-UCU-trnG-GCC	5	GGATTCGAACCTATACTGGAGG	AAGCTAATGATGCGGGTTCG	22	20	50.0	50.0	57.5	58.7	0.0	0.0	3.223181	21916	21994	175-175	1.000000	57.4
psaM-trnS-GCU_p1	psaM-trnS-GCU	1	CGTTACAGGTATTTTGGCCC	GCTCGTACAACGGATTAGCA	20	20	50.0	50.0	56.8	58.1	0.0	0.0	1.300628	23279	23666	466-490	1.000000	79.7
psaM-trnS-GCU_p2	psaM-trnS-GCU	2	CGTTACAGGTATTTTGGCCCT	GCTCGTACAACGGATTAGCA	21	20	47.6	50.0	58.3	58.1	0.0	0.0	1.364164	23279	23666	466-490	1.000000	79.1
psaM-trnS-GCU_p3	psaM-trnS-GCU	3	TCGTTACAGGTATTTTGGCCC	GCTCGTACAACGGATTAGCA	21	20	47.6	50.0	58.3	58.1	0.0	0.0	1.368851	23279	23666	467-491	1.000000	79.1
psaM-trnS-GCU_p4	psaM-trnS-GCU	4	CGTTACAGGTATTTTGGCCC	AGCTCGTACAACGGATTAGC	20	20	50.0	50.0	56.8	57.8	0.0	0.0	1.423207	23279	23666	467-491	1.000000	78.7
psaM-trnS-GCU_p5	psaM-trnS-GCU	5	CGTTACAGGTATTTTGGCCCT	AGCTCGTACAACGGATTAGC	21	20	47.6	50.0	58.3	57.8	0.0	0.0	1.486743	23279	23666	467-491	1.000000	78.2
trnS-GCU-psbI_p1	trnS-GCU-psbI	1	GCTATTCGTACCGAGGGTTC	CGTAATCCAGGACGTAAAGAGT	20	22	55.0	45.5	57.9	57.6	0.0	0.0	2.498666	23755	23789	96-96	1.000000	63.3
trnS-GCU-psbI_p2	trnS-GCU-psbI	2	TGCTAATCCGTTGTACGAGC	CGTAATCCAGGACGTAAAGAGT	20	22	50.0	45.5	58.1	57.6	0.0	0.0	2.450113	23755	23789	114-114	1.000000	63.2
trnS-GCU-psbI_p3	trnS-GCU-psbI	3	GGGTTCGAATCCCTCTCTCT	CGTAATCCAGGACGTAAAGAGT	20	22	55.0	45.5	58.2	57.6	17.2	0.0	2.584314	23755	23789	82-82	1.000000	63.2
trnS-GCU-psbI_p4	trnS-GCU-psbI	4	AGCTATTCGTACCGAGGGTT	CGTAATCCAGGACGTAAAGAGT	20	22	50.0	45.5	58.2	57.6	0.0	0.0	2.594549	23755	23789	97-97	1.000000	62.5
trnS-GCU-psbI_p5	trnS-GCU-psbI	5	GCTAATCCGTTGTACGAGCT	CGTAATCCAGGACGTAAAGAGT	20	22	50.0	45.5	57.8	57.6	0.0	0.0	2.572692	23755	23789	113-113	1.000000	62.3
psbI-psbK_p1	psbI-psbK	1	GGATTACGTCCCGGATCATT	GCTTTTGTTTGGCAAGCTTC	20	20	50.0	45.0	57.2	57.0	0.7	15.2	1.858229	23901	24232	415-465	1.000000	75.2
psbI-psbK_p2	psbI-psbK	2	GGATTACGTCCCGGATCATT	TTTGTTTGGCAAGCTTCTGT	20	20	50.0	40.0	57.2	56.7	0.7	15.2	2.180006	23901	24232	412-462	1.000000	72.7
psbI-psbK_p3	psbI-psbK	3	GGATTACGTCCCGGATCATT	GCTTTTGTTTGGCAAGCTTCT	20	21	50.0	42.9	57.2	58.4	0.7	15.2	2.279490	23901	24232	415-465	1.000000	71.8
psbI-psbK_p4	psbI-psbK	4	GGATTACGTCCCGGATCATT	TTTTGTTTGGCAAGCTTCTGT	20	21	50.0	38.1	57.2	57.4	0.7	15.2	2.441948	23901	24232	413-463	1.000000	70.6
psbI-psbK_p5	psbI-psbK	5	GGATTACGTCCCGGATCATT	TGTTTGGCAAGCTTCTGTAAG	20	21	50.0	42.9	57.2	57.0	0.7	15.2	2.822010	23901	24232	410-460	1.000000	67.6
chlB-trnK_p1	chlB-trnK	1	GCGGTAGTTTTTGGTGATGC	CAAGTCGCACTTAAAAGCCG	20	20	50.0	50.0	58.0	58.0	0.0	1.9	0.072340	26430	27112	1294-1351	1.000000	86.9
chlB-trnK_p2	chlB-trnK	2	GCCCGACAAAATGGTATTGC	GTTGGTCAGCACACAGGTTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.116963	26430	27112	1111-1168	1.000000	86.5
chlB-trnK_p3	chlB-trnK	3	CCTTCGGCAATTTTTGGGAC	GTTGGTCAGCACACAGGTTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117055	26430	27112	1459-1516	1.000000	85.7
chlB-trnK_p4	chlB-trnK	4	CTTCGGCAATTTTTGGGACC	GTTGGTCAGCACACAGGTTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117055	26430	27112	1458-1515	1.000000	85.7
chlB-trnK_p5	chlB-trnK	5	GCGGTAGTTTTTGGTGATGC	GTTGGTCAGCACACAGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061018	26430	27112	1660-1717	1.000000	85.6
trnE-UGA-trnT-GGU_p1	trnE-UGA-trnT-GGU	1	GTCGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009167	37919	39170	1346-1360	1.000000	88.4
trnE-UGA-trnT-GGU_p2	trnE-UGA-trnT-GGU	2	GTCGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.067264	37919	39170	1325-1339	1.000000	88.0
trnE-UGA-trnT-GGU_p3	trnE-UGA-trnT-GGU	3	GTCGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.089418	37919	39170	1322-1336	1.000000	87.9
trnE-UGA-trnT-GGU_p4	trnE-UGA-trnT-GGU	4	TCGCCTCCTTGAAAGAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.1	58.0	9.0	0.0	0.081239	37919	39170	1345-1359	1.000000	87.8
trnE-UGA-trnT-GGU_p5	trnE-UGA-trnT-GGU	5	GTCGCCTCCTTGAAAGAGAG	AGGCCCCTTATCAGATTCGA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.106350	37919	39170	1364-1378	1.000000	87.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GACCTCTGAGCTAAACAGGC	AAATTAAGTCCGCAGCGTCT	20	20	55.0	45.0	58.0	57.9	0.0	15.0	0.111900	51644	51887	295-304	1.000000	87.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GACCTCTGAGCTAAACAGGC	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.0	57.8	0.0	15.0	0.222445	51644	51887	293-302	1.000000	86.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GACCTCTGAGCTAAACAGGC	TACCAATTTCGCCATACCCC	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.368844	51644	51887	276-285	1.000000	86.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	AAATTAAGTCCGCAGCGTCT	20	20	55.0	45.0	58.2	57.9	0.0	15.0	0.253313	51644	51887	336-345	1.000000	85.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GAGTCGAACCGATGACCATC	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.2	57.8	0.0	15.0	0.363857	51644	51887	334-343	1.000000	84.7
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	GTTCAAGTCCCTCTACCCCC	AATCCCCTGCTCTACCAACT	20	20	60.0	50.0	59.1	58.0	0.0	0.0	1.117074	52323	52419	151-154	1.000000	75.3
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GGGTTCAAGTCCCTCTACCC	AATCCCCTGCTCTACCAACT	20	20	60.0	50.0	59.1	58.0	0.0	0.0	1.117074	52323	52419	153-156	1.000000	75.2
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	GGTTCAAGTCCCTCTACCCC	AATCCCCTGCTCTACCAACT	20	20	60.0	50.0	59.1	58.0	0.0	0.0	1.117074	52323	52419	152-155	1.000000	75.2
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GTTCAAGTCCCTCTACCCCC	TCAATCCCCTGCTCTACCAA	20	20	60.0	50.0	59.1	58.0	0.0	0.0	1.124248	52323	52419	153-156	1.000000	75.2
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	GTTCAAGTCCCTCTACCCCC	TTCAATCCCCTGCTCTACCA	20	20	60.0	50.0	59.1	58.0	0.0	0.0	1.124248	52323	52419	154-157	1.000000	75.1
psbJ-psbL_p1	psbJ-psbL	1	CTTCCGGTAGTATTGGCCAT	TTTGTACTTGCTGTTCTTTTT	20	21	50.0	28.6	57.1	52.4	6.1	0.0	7.561914	64672	64791	182-185	1.000000	46.1
psbJ-psbL_p2	psbJ-psbL	2	CCTTCCGGTAGTATTGGCCA	TTTGTACTTGCTGTTCTTTTT	20	21	55.0	28.6	59.2	52.4	0.0	0.0	7.796475	64672	64791	183-186	1.000000	46.1
psbJ-psbL_p3	psbJ-psbL	3	CTTCCGGTAGTATTGGCCAT	ATTTGTACTTGCTGTTCTTTT	20	21	50.0	28.6	57.1	52.1	6.1	0.0	7.797943	64672	64791	183-186	1.000000	46.1
psbJ-psbL_p4	psbJ-psbL	4	TCCTTCCGGTAGTATTGGCC	TTTGTACTTGCTGTTCTTTTT	20	21	55.0	28.6	58.9	52.4	7.4	0.0	7.505454	64672	64791	184-187	1.000000	46.0
psbJ-psbL_p5	psbJ-psbL	5	TCCTTCCGGTAGTATTGGCC	ATTTGTACTTGCTGTTCTTTT	20	21	55.0	28.6	58.9	52.1	7.4	0.0	7.741483	64672	64791	185-188	1.000000	46.0
psaJ-rpl33_p1	psaJ-rpl33	1	ACGTTGTGGTTCGGATTCTT	CGACGACATCTTTTTCCAAA	20	20	45.0	40.0	57.8	54.6	0.0	0.0	3.679961	66979	67129	318-319	1.000000	55.1
psaJ-rpl33_p2	psaJ-rpl33	2	CGGATTCTTGGCCGGTTTAT	CGACGACATCTTTTTCCAAA	20	20	50.0	40.0	58.3	54.6	1.9	0.0	3.754566	66979	67129	307-308	1.000000	54.6
psaJ-rpl33_p3	psaJ-rpl33	3	ACGTTGTGGTTCGGATTCTT	CGACGACATCTTTTTCCAAAA	20	21	45.0	38.1	57.8	55.4	0.0	0.0	3.864203	66979	67129	318-319	1.000000	53.6
psaJ-rpl33_p4	psaJ-rpl33	4	CGGATTCTTGGCCGGTTTAT	CGACGACATCTTTTTCCAAAA	20	21	50.0	38.1	58.3	55.4	1.9	0.0	3.938807	66979	67129	307-308	1.000000	53.2
psaJ-rpl33_p5	psaJ-rpl33	5	GCTACGTTGTGGTTCGGATT	CGACGACATCTTTTTCCAAA	20	20	50.0	40.0	58.6	54.6	0.0	0.0	3.994541	66979	67129	321-322	1.000000	52.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGGGCATCGACAAAAATCA	CTAACATTGCACCAGGAGCT	20	20	45.0	50.0	58.1	57.9	0.0	10.5	0.203296	95839	96624	1712-1746	0.666667	70.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGGGGCATCGACAAAAATCA	CGGAGCCAACTAGCTGAAAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.113333	95839	96624	1388-1388	0.333333	52.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGACACGACGTAAATTGGGG	CGGAGCCAACTAGCTGAAAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.118788	95839	96624	1403-1403	0.333333	52.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGGGGCATCGACAAAAATCA	GAGCTAACATTGCACCAGGA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.197355	95839	96624	1749-1749	0.333333	51.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGACACGACGTAAATTGGGG	GAGCTAACATTGCACCAGGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.202810	95839	96624	1764-1764	0.333333	51.7
ycf1_p1	ycf1	1	TCTTGCCAATACGGGACTTG	TACGGATTCCTCACCACACT	20	20	50.0	50.0	57.9	58.1	0.0	0.3	0.174929	109758	109877	363-363	1.000000	83.2
ycf1_p2	ycf1	2	CGAAAACTGCTACACTGGGT	TACGGATTCCTCACCACACT	20	20	50.0	50.0	58.1	58.1	0.0	0.3	0.188026	109758	109877	856-856	1.000000	81.8
ycf1_p3	ycf1	3	TCTTGCCAATACGGGACTTG	CTCACCACACTGCCCATTAG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.365189	109758	109877	354-354	1.000000	81.8
ycf1_p4	ycf1	4	TCGAAAACTGCTACACTGGG	TACGGATTCCTCACCACACT	20	20	50.0	50.0	57.8	58.1	0.0	0.3	0.230797	109758	109877	857-857	1.000000	81.4
ycf1_p5	ycf1	5	CCGCAAAACCCACCTAGAAT	TACGGATTCCTCACCACACT	20	20	50.0	50.0	58.2	58.1	0.0	0.3	0.232791	109758	109877	440-440	0.333333	51.5
ycf1-chlN_p1	ycf1-chlN	1	TCTTGCCAATACGGGACTTG	CAGATCTACCGGCCTTAGGA	20	20	50.0	55.0	57.9	58.0	0.0	0.7	0.111152	110034	110154	1257-1266	1.000000	82.5
ycf1-chlN_p2	ycf1-chlN	2	AGTGTGGTGAGGAATCCGTA	CAGATCTACCGGCCTTAGGA	20	20	50.0	55.0	58.1	58.0	0.0	0.7	0.070180	110034	110154	914-923	1.000000	82.2
ycf1-chlN_p3	ycf1-chlN	3	AGTGTGGTGAGGAATCCGTA	GGGTTGGTTACCTGCTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	2.1	0.077272	110034	110154	940-949	1.000000	82.1
ycf1-chlN_p4	ycf1-chlN	4	TCTTGCCAATACGGGACTTG	TTCGGTGTGGAATGATCGTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.112533	110034	110154	948-957	0.666667	68.8
ycf1-chlN_p5	ycf1-chlN	5	AGTGTGGTGAGGAATCCGTA	TTCGGTGTGGAATGATCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	110034	110154	605-614	0.666667	68.6