primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6021	7501	1127-1604	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6021	7501	1131-1608	1.000000	85.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6021	7501	1119-1596	1.000000	85.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	8.1	16.9	0.938588	6021	7501	1126-1603	1.000000	84.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GGTTTTGGTCCCGTTATTCG	20	20	45.0	50.0	58.1	57.1	8.1	0.0	1.045283	6021	7501	1147-1624	1.000000	83.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	18	21	61.1	38.1	56.4	55.5	0.0	0.0	7.134070	8848	10345	1024-2151	0.961538	50.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCACTCAGCCATCTCTCC	AGAACGAATCACACTTTTACCAC	18	23	61.1	39.1	56.4	57.3	0.0	0.0	7.266785	8848	10345	1027-2154	0.961538	50.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	18	23	61.1	39.1	56.4	57.3	0.0	0.0	7.266785	8848	10345	1026-2153	0.961538	50.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	18	22	61.1	36.4	56.4	56.2	0.0	0.0	7.374540	8848	10345	1025-2152	0.961538	50.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	18	20	61.1	45.0	56.4	54.0	0.0	0.0	7.609303	8848	10345	1010-2137	0.961538	50.5
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	CCATGGAGGGTCATCATTGA	20	20	45.0	50.0	58.1	57.0	0.0	5.6	1.139756	15140	16903	1150-2484	0.923077	79.8
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TCCATGGAGGGTCATCATTG	20	20	45.0	50.0	58.1	57.0	0.0	11.4	1.139756	15140	16903	1151-2485	0.923077	79.8
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	CCATGGAGGGTCATCATTGA	20	20	50.0	50.0	57.8	57.0	0.0	5.6	1.250363	15140	16903	1167-2501	0.923077	78.9
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	TCCATGGAGGGTCATCATTG	20	20	50.0	50.0	57.8	57.0	0.0	11.4	1.250363	15140	16903	1168-2502	0.923077	78.9
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	CCATGGAGGGTCATCATTGA	20	20	50.0	50.0	58.3	57.0	0.0	5.6	1.298138	15140	16903	1162-2496	0.923077	78.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTTCCATCATTCCGGAGCA	GCGACACCCAGATTTGAACT	20	20	45.0	50.0	57.3	58.5	3.3	0.0	1.210998	29211	30385	1265-1360	0.923077	79.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTTCCATCATTCCGGAGCAT	GCGACACCCAGATTTGAACT	20	20	45.0	50.0	57.3	58.5	3.3	0.0	1.210998	29211	30385	1264-1359	0.923077	79.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTTCCATCATTCCGGAGCA	TAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.3	57.5	3.3	14.6	1.259860	29211	30385	1240-1335	0.923077	78.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTTCCATCATTCCGGAGCAT	TAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.3	57.5	3.3	14.6	1.259860	29211	30385	1239-1334	0.923077	78.8
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTTCCATCATTCCGGAGCAT	CCTTACCACTTGGCCATGTC	20	20	45.0	55.0	57.3	58.5	3.3	20.8	1.271081	29211	30385	1218-1313	0.923077	78.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAGAAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.1	0.0	0.063299	49264	50412	1107-1531	0.923077	88.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AGAGAAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.1	0.0	0.128108	49264	50412	1115-1539	0.923077	87.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49264	50412	1056-1480	0.923077	87.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	49264	50412	1087-1511	0.923077	87.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49264	50412	1064-1488	0.923077	87.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	ATAGGTTGGCCCCTACGTAA	20	20	50.0	50.0	58.1	57.8	0.0	9.7	0.231273	51524	52213	776-811	0.961538	88.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.283612	51524	52213	784-819	0.961538	88.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	GATAGGTTGGCCCCTACGTA	20	20	50.0	55.0	58.1	58.3	0.0	9.7	0.343555	51524	52213	777-812	0.923077	86.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CGGGAAATAGTCGGGATAGC	ATAGGTTGGCCCCTACGTAA	20	20	55.0	50.0	57.6	57.8	0.0	9.7	0.544785	51524	52213	824-859	0.961538	86.1
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TAGGTTGGCCCCTACGTAAG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.563506	51524	52213	775-810	0.923077	84.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	22	20	36.4	50.0	56.1	57.8	0.0	0.0	4.083948	53909	55955	789-2350	0.961538	57.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	22	20	36.4	55.0	56.1	57.5	0.0	0.0	4.427720	53909	55955	788-2349	0.961538	55.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	36.4	55.0	56.1	58.7	0.0	0.0	4.570014	53909	55955	777-2338	0.961538	53.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	54.6	57.8	0.0	0.0	4.585537	53909	55955	788-2349	0.961538	53.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	56.6	57.8	0.0	0.0	4.587896	53909	55955	789-2350	0.961538	53.8
accD-psaI_p1	accD-psaI	1	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.080258	63453	64896	1726-2178	0.961538	89.8
accD-psaI_p2	accD-psaI	2	GTATGTGCTTCTGGAGGAGC	CACACGGAGCCTAGACAATC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.125360	63453	64896	1777-2229	0.961538	89.5
accD-psaI_p3	accD-psaI	3	GTATGTGCTTCTGGAGGAGC	AGACCTGCGATCCCATAGAA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.141359	63453	64896	2380-2838	0.961538	89.3
accD-psaI_p4	accD-psaI	4	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	63453	64896	1376-1828	0.961538	89.3
accD-psaI_p5	accD-psaI	5	GTATGTGCTTCTGGAGGAGC	TTGTTTTTCGAGACCCCGTT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.094506	63453	64896	2241-2675	0.923077	88.2
psaI-ycf4_p1	psaI-ycf4	1	ATTTCCGGCAATTGCAATGG	AAAAAGGATACAGGCCCAGC	20	20	45.0	50.0	58.0	58.1	30.3	0.0	0.174371	65008	65399	535-546	0.961538	87.4
psaI-ycf4_p2	psaI-ycf4	2	GGCAATTGCAATGGCTTCTT	AAAAAGGATACAGGCCCAGC	20	20	45.0	50.0	57.9	58.1	32.4	0.0	0.250905	65008	65399	529-540	0.961538	86.8
psaI-ycf4_p3	psaI-ycf4	3	ATTTCCGGCAATTGCAATGG	TTGTTTTTCGAGACCCCGTT	20	20	45.0	45.0	58.0	58.0	30.3	0.0	0.068254	65008	65399	506-517	0.923077	86.2
psaI-ycf4_p4	psaI-ycf4	4	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	20	20	45.0	45.0	57.9	58.0	32.4	0.0	0.144788	65008	65399	500-511	0.923077	85.7
psaI-ycf4_p5	psaI-ycf4	5	GGCCTAGTATTTCCGGCAAT	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	57.7	58.0	4.4	0.0	0.313195	65008	65399	514-525	0.923077	84.3
petA-psbJ_p1	petA-psbJ	1	TTTGGCGCAAGTCTTTTTGG	CATATTCCGGCTTGGGTTCA	20	20	45.0	50.0	58.1	57.9	10.8	0.0	0.118088	67843	69037	1209-1451	0.846154	84.9
petA-psbJ_p2	petA-psbJ	2	TTTGGCGCAAGTCTTTTTGG	GTTCATATTCCGGCTTGGGT	20	20	45.0	50.0	58.1	57.9	10.8	0.0	0.119536	67843	69037	1212-1454	0.846154	84.9
petA-psbJ_p3	petA-psbJ	3	CCCATTACGTGTCCAAGGTC	CATATTCCGGCTTGGGTTCA	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.317172	67843	69037	1195-1496	0.884615	84.8
petA-psbJ_p4	petA-psbJ	4	GACCCATTACGTGTCCAAGG	CATATTCCGGCTTGGGTTCA	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.317172	67843	69037	1197-1498	0.884615	84.8
petA-psbJ_p5	petA-psbJ	5	CCCATTACGTGTCCAAGGTC	GTTCATATTCCGGCTTGGGT	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.318620	67843	69037	1198-1499	0.884615	84.8
psaJ-rpl33_p1	psaJ-rpl33	1	TGGTTTGGGTCTTTAGCAGG	AATGCCCGGCGATTCTTTAT	20	20	50.0	45.0	57.7	58.0	0.0	9.3	0.305108	72360	72980	722-888	1.000000	89.6
psaJ-rpl33_p2	psaJ-rpl33	2	TGGTTTGGGTCTTTAGCAGG	ATGCCCGGCGATTCTTTATT	20	20	50.0	45.0	57.7	58.0	0.0	9.3	0.305108	72360	72980	721-887	1.000000	89.6
psaJ-rpl33_p3	psaJ-rpl33	3	GGTTTGGGTCTTTAGCAGGT	AATGCCCGGCGATTCTTTAT	20	20	50.0	45.0	57.7	58.0	0.0	9.3	0.306721	72360	72980	721-887	1.000000	89.5
psaJ-rpl33_p4	psaJ-rpl33	4	GGTTTGGGTCTTTAGCAGGT	ATGCCCGGCGATTCTTTATT	20	20	50.0	45.0	57.7	58.0	0.0	9.3	0.306721	72360	72980	720-886	1.000000	89.5
psaJ-rpl33_p5	psaJ-rpl33	5	TGGTTTGGGTCTTTAGCAGG	AAATGCCCGGCGATTCTTTA	20	20	50.0	45.0	57.7	58.2	0.0	9.3	0.528682	72360	72980	723-889	1.000000	87.8
petD-rpoA_p1	petD-rpoA	1	TAATGGTTTCAGTACCGGCG	AGAGATGCATCCATTTGCGT	20	20	50.0	45.0	58.0	58.0	0.0	23.1	0.061381	83094	83267	1065-1100	1.000000	89.4
petD-rpoA_p2	petD-rpoA	2	TAATGGTTTCAGTACCGGCG	CATTTGCGTCAAGGGTCCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.120887	83094	83267	1054-1089	1.000000	89.0
petD-rpoA_p3	petD-rpoA	3	CTCGAGCCGGATGATGAAAA	AGAGATGCATCCATTTGCGT	20	20	50.0	45.0	58.1	58.0	5.0	23.1	0.108470	83094	83267	1435-1470	1.000000	87.6
petD-rpoA_p4	petD-rpoA	4	TAATGGTTTCAGTACCGGCG	GGATACCGTACGAAACCCAC	20	20	50.0	55.0	58.0	58.1	0.0	18.8	0.086376	83094	83267	909-944	0.923077	85.2
petD-rpoA_p5	petD-rpoA	5	CTCGAGCCGGATGATGAAAA	GGATACCGTACGAAACCCAC	20	20	50.0	55.0	58.1	58.1	5.0	18.8	0.133464	83094	83267	1279-1314	0.923077	83.5
ndhF-rpl32_p1	ndhF-rpl32	1	TTTTGACAAGCACTTGCAGC	AAAGGGCTTTTACTGCAGCT	20	20	45.0	45.0	58.1	58.0	10.3	15.3	0.075847	118312	119199	1515-1629	0.961538	89.9
ndhF-rpl32_p2	ndhF-rpl32	2	CTCCCACGAGTTCCCAAAAA	AAAGGGCTTTTACTGCAGCT	20	20	50.0	45.0	58.0	58.0	0.0	15.3	0.025416	118312	119199	1451-1565	0.923077	88.7
ndhF-rpl32_p3	ndhF-rpl32	3	ATGCTTTTTGTTGCTGTCGG	AAAGGGCTTTTACTGCAGCT	20	20	45.0	45.0	57.9	58.0	0.0	15.3	0.139672	118312	119199	1092-1206	0.884615	86.3
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAGCACTTGCAGCAATTG	AAAGGGCTTTTACTGCAGCT	20	20	45.0	45.0	58.1	58.0	18.8	15.3	0.140400	118312	119199	1510-1563	0.269231	54.3
ndhF-rpl32_p5	ndhF-rpl32	5	GACAAGCACTTGCAGCAATT	AAAGGGCTTTTACTGCAGCT	20	20	45.0	45.0	57.9	58.0	8.7	15.3	0.145558	118312	119199	1511-1564	0.269231	54.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	20	20	30.0	50.0	53.4	57.9	12.1	0.0	4.747833	119374	119965	533-719	0.961538	52.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	20	20	30.0	45.0	53.4	57.8	12.1	0.0	4.808560	119374	119965	526-712	0.961538	52.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTTTGTGCGACAAACAAAT	CCTAAGAGCAGCGTGTCTAC	20	20	30.0	55.0	53.4	57.8	12.1	0.0	4.819851	119374	119965	532-718	0.961538	51.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	20	20	30.0	55.0	53.4	57.8	12.1	0.0	4.819851	119374	119965	531-717	0.961538	51.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTTTGTGCGACAAACAAAT	GAGCAGCGTGTCTACCAATT	20	20	30.0	50.0	53.4	58.3	12.1	0.0	4.896703	119374	119965	527-713	0.961538	51.3
psaC-ndhE_p1	psaC-ndhE	1	CATAAATTTTTACTGAATGTGACA	CGATCCACCGGAACAGAAAA	24	20	25.0	50.0	52.3	58.2	0.0	9.5	9.855741	123231	123887	678-822	1.000000	52.0
psaC-ndhE_p2	psaC-ndhE	2	TCATAAATTTTTACTGAATGTGAC	CGATCCACCGGAACAGAAAA	24	20	25.0	50.0	52.1	58.2	0.0	9.5	10.103061	123231	123887	679-823	1.000000	52.0
psaC-ndhE_p3	psaC-ndhE	3	CATAAATTTTTACTGAATGTGACA	CTGAAGCAGCTATTGGGCTA	24	20	25.0	50.0	52.3	57.4	0.0	0.0	10.286590	123231	123887	711-855	1.000000	52.0
psaC-ndhE_p4	psaC-ndhE	4	CATAAATTTTTACTGAATGTGACA	TGAAGCAGCTATTGGGCTAG	24	20	25.0	50.0	52.3	57.4	0.0	0.0	10.286590	123231	123887	710-854	1.000000	52.0
psaC-ndhE_p5	psaC-ndhE	5	CATAAATTTTTACTGAATGTGACA	ATTGTTTCGTCGATCCACCG	24	20	25.0	50.0	52.3	58.6	0.0	0.0	10.303104	123231	123887	688-832	1.000000	52.0
rps15-ycf1_p1	rps15-ycf1	1	AACACCATGCATTGATGGGT	AAGAAAAAGCGCCAAACACC	20	20	45.0	45.0	58.1	58.1	8.9	2.3	0.124034	129862	130407	1317-1464	0.769231	81.8
rps15-ycf1_p2	rps15-ycf1	2	ACCATGCATTGATGGGTGTT	AAGAAAAAGCGCCAAACACC	20	20	45.0	45.0	58.1	58.1	8.9	2.3	0.124034	129862	130407	1314-1461	0.769231	81.8
rps15-ycf1_p3	rps15-ycf1	3	CACCGTCGAGAGTAACGATC	AAGAAAAAGCGCCAAACACC	20	20	55.0	45.0	57.9	58.1	0.0	2.3	0.153750	129862	130407	1345-1492	0.769231	81.5
rps15-ycf1_p4	rps15-ycf1	4	GGCTCAGCTCCAGCATAATT	AGGGGAGAAATGCGTCTAGA	20	20	50.0	50.0	58.0	57.8	14.7	0.0	0.182266	129862	130407	1729-1882	0.653846	76.7
rps15-ycf1_p5	rps15-ycf1	5	GGCTCAGCTCCAGCATAATT	AAGAAAAAGCGCCAAACACC	20	20	50.0	45.0	58.0	58.1	14.7	2.3	0.086957	129862	130407	1570-1717	0.615385	75.9