primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6038	6756	792-1094	1.000000	87.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6038	6756	803-1105	1.000000	87.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6038	6756	796-1098	1.000000	86.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	6038	6756	807-1109	1.000000	86.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	6038	6756	798-1100	1.000000	84.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8199	9519	686-1390	1.000000	63.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8199	9519	685-1389	1.000000	63.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8199	9519	689-1393	1.000000	62.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8199	9519	688-1392	1.000000	62.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8199	9519	687-1391	1.000000	62.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10295	10450	210-243	1.000000	73.3
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10295	10450	213-246	1.000000	73.0
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10295	10450	216-249	1.000000	72.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10295	10450	219-252	1.000000	72.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.8	56.9	14.7	0.0	2.265991	10295	10450	231-264	1.000000	71.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46509	47192	700-769	1.000000	88.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	46509	47192	708-777	1.000000	87.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CTAACCTCTGAGCTAAGCGG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.5	58.0	5.5	0.0	0.505133	46509	47192	660-729	1.000000	86.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	20	20	50.0	50.0	57.8	57.8	15.5	0.0	0.465562	46509	47192	723-792	1.000000	86.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	15.5	10.2	0.480302	46509	47192	714-783	1.000000	86.1
trnF-GAA-trnV-UAC_p1	trnF-GAA-trnV-UAC	1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.249179	48285	49399	428-1182	1.000000	90.0
trnF-GAA-trnV-UAC_p2	trnF-GAA-trnV-UAC	2	GGAAATGGTCGGGATAGCTC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.368624	48285	49399	474-1228	1.000000	89.1
trnF-GAA-trnV-UAC_p3	trnF-GAA-trnV-UAC	3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	48285	49399	471-1225	1.000000	88.8
trnF-GAA-trnV-UAC_p4	trnF-GAA-trnV-UAC	4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	48285	49399	470-1224	1.000000	88.8
trnF-GAA-trnV-UAC_p5	trnF-GAA-trnV-UAC	5	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	0.0	0.0	0.592951	48285	49399	427-1181	1.000000	87.3
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	58.4	58.0	13.2	0.0	0.442584	50306	50574	304-466	1.000000	87.3
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.474048	50306	50574	296-458	1.000000	87.2
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	AGGACACGAGTAGAAGCTGT	20	20	50.0	50.0	58.4	58.1	13.2	0.0	0.498909	50306	50574	290-452	1.000000	87.1
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TATTGCTTTCATACGGCGGG	GGACACGAGTAGAAGCTGTC	20	20	50.0	55.0	58.4	57.7	13.2	0.0	0.676973	50306	50574	289-451	1.000000	85.7
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	20	20	50.0	55.0	58.4	58.3	13.2	0.0	0.671029	50306	50574	303-465	1.000000	85.5
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.174567	70442	71433	1056-1172	1.000000	88.5
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	70442	71433	972-1088	1.000000	88.5
clpP-psbB_p3	clpP-psbB	3	TATACGTCAACCCAAGCTGC	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.192157	70442	71433	1051-1167	1.000000	88.4
clpP-psbB_p4	clpP-psbB	4	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.255834	70442	71433	967-1083	1.000000	88.3
clpP-psbB_p5	clpP-psbB	5	CTTTTGGAACACCAATGGGC	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	57.8	58.2	3.6	0.0	0.341941	70442	71433	1002-1118	1.000000	87.5
psbB-psbT_p1	psbB-psbT	1	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	22	24	50.0	37.5	57.6	57.5	0.0	0.0	6.858434	72961	73367	428-555	1.000000	51.4
psbB-psbT_p2	psbB-psbT	2	AGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	22	24	45.5	37.5	57.7	57.5	0.9	0.0	6.802790	72961	73367	429-556	1.000000	51.3
psbB-psbT_p3	psbB-psbT	3	AGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	22	24	45.5	37.5	57.7	57.3	0.9	0.0	7.054142	72961	73367	430-557	1.000000	51.3
psbB-psbT_p4	psbB-psbT	4	TGCTCAAGTAGAATTTGGAGCA	GGAATGTATAAACCAATGCTTCCA	22	24	40.9	37.5	58.0	57.5	9.8	0.0	6.483786	72961	73367	462-589	1.000000	50.9
psbB-psbT_p5	psbB-psbT	5	TGCTCAAGTAGAATTTGGAGCA	AGGAATGTATAAACCAATGCTTCC	22	24	40.9	37.5	58.0	57.3	9.8	0.0	6.735139	72961	73367	463-590	1.000000	50.9
rpoA-rps11_p1	rpoA-rps11	1	AAGTGGGGACAGAACGAAAC	GGAACACCTTATGCTGCTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.150688	78254	78345	415-424	1.000000	83.0
rpoA-rps11_p2	rpoA-rps11	2	TCGCATTTCGAACAGGCATA	GGAACACCTTATGCTGCTCA	20	20	45.0	50.0	58.0	57.9	13.3	0.0	0.130998	78254	78345	851-860	1.000000	81.6
rpoA-rps11_p3	rpoA-rps11	3	TCGCATTTCGAACAGGCATA	AAGAGATGCGGCATTACGAG	20	20	45.0	50.0	58.0	58.1	13.3	17.8	0.159047	78254	78345	747-756	1.000000	81.5
rpoA-rps11_p4	rpoA-rps11	4	GTGTCCTCTTCCTCTGCATG	GGAACACCTTATGCTGCTCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135216	78254	78345	1014-1023	1.000000	81.3
rpoA-rps11_p5	rpoA-rps11	5	GTGTCCTCTTCCTCTGCATG	AAGAGATGCGGCATTACGAG	20	20	55.0	50.0	58.0	58.1	0.0	17.8	0.163264	78254	78345	910-919	1.000000	81.2
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	97645	99693	1890-2145	1.000000	84.3
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	97645	99693	1889-2144	1.000000	84.3
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	97645	99693	1894-2149	1.000000	84.2
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	97645	99693	1892-2147	1.000000	83.3
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	20	20	45.0	55.0	57.7	57.7	16.5	5.4	0.606988	97645	99693	1875-2130	1.000000	82.8
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CAAAGTGAGCCCTTTCCCTT	TGCTCCCGGTTACTATGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145781	108172	108974	1197-1197	0.200000	46.8
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GCAAAAATGCGTTCCTCCTC	TGCTCCCGGTTACTATGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.162093	108172	108974	1437-1437	0.200000	46.7
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGCAGAATAGCAGAGCAAGT	TGCTCCCGGTTACTATGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.180235	108172	108974	1475-1475	0.200000	46.6
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	TATGCCGAGGTATTGAGGGT	TGCTCCCGGTTACTATGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.241815	108172	108974	1308-1308	0.200000	46.1
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CGGCCGCAAAGATATTGAAC	TGCTCCCGGTTACTATGTCA	20	20	50.0	50.0	57.9	58.1	14.7	0.0	0.250028	108172	108974	1638-1638	0.200000	46.0
ndhF-rpl32_p1	ndhF-rpl32	1	AGGGCCCTCTTATCTCTTCC	CTTTTTGACTGTCCCGTGGA	20	20	55.0	50.0	57.9	58.0	22.3	5.2	0.148960	109068	109873	1003-1467	0.600000	74.8
ndhF-rpl32_p2	ndhF-rpl32	2	GGGCCCTCTTATCTCTTCCT	CTTTTTGACTGTCCCGTGGA	20	20	55.0	50.0	57.9	58.0	17.0	5.2	0.148960	109068	109873	1002-1466	0.600000	74.8
ndhF-rpl32_p3	ndhF-rpl32	3	TACGAGCTCGGGTTATGGAA	CTTTTTGACTGTCCCGTGGA	20	20	50.0	50.0	58.2	58.0	36.9	5.2	0.271713	109068	109873	980-1444	0.600000	73.8
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTATCAACTGCACCGA	CTTTTTGACTGTCCCGTGGA	20	20	50.0	50.0	57.6	58.0	0.0	5.2	0.418290	109068	109873	1404-1404	0.200000	44.7
ndhF-rpl32_p5	ndhF-rpl32	5	AGGAAGTATCAACTGCACCG	CTTTTTGACTGTCCCGTGGA	20	20	50.0	50.0	57.6	58.0	0.0	5.2	0.424867	109068	109873	1405-1405	0.200000	44.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.213227	110787	110927	389-404	1.000000	86.4
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TAAGCCGCCATGGTGAAATT	GTCCCGAAGAAATCCAACGA	20	20	45.0	50.0	58.2	57.9	27.1	0.0	0.242943	110787	110927	411-426	1.000000	85.7
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TAGACACGCTGCTCTTAGGA	CTGCTCCAGAAAGAATGCCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.250050	110787	110927	920-935	1.000000	82.7
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TAGACACGCTGCTCTTAGGA	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.193111	110787	110927	536-546	0.800000	75.9
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TAAGCCGCCATGGTGAAATT	TGGCGAAACCTTGGGTAAAA	20	20	45.0	45.0	58.2	57.9	27.1	0.0	0.222828	110787	110927	558-568	0.800000	75.5
ndhI-rps15_p1	ndhI-rps15	1	CCGTTCATAAACATCGCAGC	GGCAATTTTTGAATGAGGGCA	20	21	50.0	42.9	57.9	57.9	0.0	0.0	1.147740	115504	116748	2233-2233	0.200000	38.8
ndhI-rps15_p2	ndhI-rps15	2	ACCCGTTCATAAACATCGCA	GGCAATTTTTGAATGAGGGCA	20	21	45.0	42.9	57.9	57.9	0.0	0.0	1.158658	115504	116748	2235-2235	0.200000	38.7
ndhI-rps15_p3	ndhI-rps15	3	CTTGCGGCTCGTATTGTTTG	GGCAATTTTTGAATGAGGGCA	20	21	50.0	42.9	58.1	57.9	0.0	0.0	1.174390	115504	116748	1940-1940	0.200000	38.6
ndhI-rps15_p4	ndhI-rps15	4	AAAATTCCGGACCATGACGT	GGCAATTTTTGAATGAGGGCA	20	21	45.0	42.9	57.8	57.9	1.8	0.0	1.250789	115504	116748	2355-2355	0.200000	38.0
ndhI-rps15_p5	ndhI-rps15	5	TTCCGGACCATGACGTATCT	GGCAATTTTTGAATGAGGGCA	20	21	50.0	42.9	58.2	57.9	1.8	0.0	1.285023	115504	116748	2351-2351	0.200000	37.7
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	131438	133486	1890-2145	1.000000	83.8
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	131438	133486	1894-2149	1.000000	83.8
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	131438	133486	1889-2144	1.000000	83.8
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	131438	133486	1892-2147	1.000000	82.9
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	131438	133486	1875-2130	1.000000	82.4