primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf80-trnR-CCG_p1	ycf80-trnR-CCG	1	TCAAGCCTTTTCGCCATACA	CTGGTGGTCGAAGGTTCAAA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.230870	6939	7708	1261-1261	0.333333	51.5
ycf80-trnR-CCG_p2	ycf80-trnR-CCG	2	TCAAGCCTTTTCGCCATACA	ATCGGGCATGTAACTCAGTG	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.508589	6939	7708	1303-1303	0.333333	49.3
ycf80-trnR-CCG_p3	ycf80-trnR-CCG	3	TCAAGCCTTTTCGCCATACA	GATTCCGATTCTGGTGGTCG	20	20	45.0	55.0	57.8	58.4	0.0	0.0	0.620745	6939	7708	1271-1271	0.333333	48.4
ycf80-trnR-CCG_p4	ycf80-trnR-CCG	4	TCAAGCCTTTTCGCCATACA	ATTCTGGTGGTCGAAGGTTC	20	20	45.0	50.0	57.8	57.5	0.0	0.0	0.667073	6939	7708	1264-1264	0.333333	48.0
ycf80-trnR-CCG_p5	ycf80-trnR-CCG	5	TCAAGCCTTTTCGCCATACA	GATTCTGGTGGTCGAAGGTT	20	20	45.0	50.0	57.8	57.5	0.0	0.0	0.667073	6939	7708	1265-1265	0.333333	48.0
ycf20-cpcG_p1	ycf20-cpcG	1	ACAACAAGAAGGTATGCAGCA	TATTGTGGCCAGGTCCTGTA	21	20	42.9	50.0	58.1	58.0	0.0	17.9	1.177750	9223	9881	1474-1480	0.666667	69.2
ycf20-cpcG_p2	ycf20-cpcG	2	ACAAGAAGGTATGCAGCATGT	TATTGTGGCCAGGTCCTGTA	21	20	42.9	50.0	57.9	58.0	18.8	17.9	1.117774	9223	9881	1471-1471	0.333333	44.4
ycf20-cpcG_p3	ycf20-cpcG	3	ACAAGAAGGTATGCAGCATGT	GGCCAGGTCCTGTAAATCAG	21	20	42.9	55.0	57.9	58.0	18.8	17.9	1.121607	9223	9881	1465-1465	0.333333	44.4
ycf20-cpcG_p4	ycf20-cpcG	4	ACAAGAAGGTATGCAGCATGT	ACAAAGGGTTTTAGGGCGAG	21	20	42.9	50.0	57.9	58.1	18.8	0.0	1.171847	9223	9881	1728-1728	0.333333	44.0
ycf20-cpcG_p5	ycf20-cpcG	5	ACAACAAGAAGGTATGCAGCA	GGCCAGGTCCTGTAAATCAG	21	20	42.9	55.0	58.1	58.0	0.0	17.9	1.181582	9223	9881	1468-1468	0.333333	43.9
ycf22-psaC_p1	ycf22-psaC	1	CGCTGCCCTGACTAAATCAT	TGGGTGTAAGCGTTGTGAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.080776	15938	17085	1551-1562	0.666667	70.4
ycf22-psaC_p2	ycf22-psaC	2	CGCTGCCCTGACTAAATCAT	TTGGGTGTAAGCGTTGTGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.080776	15938	17085	1552-1563	0.666667	70.4
ycf22-psaC_p3	ycf22-psaC	3	CGCTGCCCTGACTAAATCAT	TGCAGGTATGACAGGTGGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.095379	15938	17085	2179-2190	0.666667	69.6
ycf22-psaC_p4	ycf22-psaC	4	CGCTGCCCTGACTAAATCAT	GGACGTAACATAGGTGCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.105026	15938	17085	2193-2204	0.666667	69.5
ycf22-psaC_p5	ycf22-psaC	5	CGCTGCCCTGACTAAATCAT	GCCAAGCAGGACGTAACATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059500	15938	17085	2201-2201	0.333333	52.9
gltB-rpoZ_p1	gltB-rpoZ	1	AACCAACACCACATGCATCT	TGGTTGAACAAGCTGTGGTT	20	20	45.0	45.0	58.0	58.2	0.0	15.8	0.154345	22115	22486	1079-1102	1.000000	88.4
gltB-rpoZ_p2	gltB-rpoZ	2	AACCAACACCACATGCATCT	AGGACACTAGAGTCTCCAGC	20	20	45.0	55.0	58.0	57.9	0.0	18.1	0.138162	22115	22486	1209-1209	0.666667	67.1
gltB-rpoZ_p3	gltB-rpoZ	3	AACCAACACCACATGCATCT	GGACACTAGAGTCTCCAGCA	20	20	45.0	55.0	58.0	58.2	0.0	18.1	0.159615	22115	22486	1208-1208	0.666667	66.9
gltB-rpoZ_p4	gltB-rpoZ	4	AACCAACACCACATGCATCT	TTGTGTAAGCATTGCCACCT	20	20	45.0	45.0	58.0	58.0	0.0	4.7	0.006396	22115	22486	1121-1121	0.333333	53.3
gltB-rpoZ_p5	gltB-rpoZ	5	TTATACCTGCCCCATCACCA	TTGTGTAAGCATTGCCACCT	20	20	50.0	45.0	58.1	58.0	0.0	4.7	0.105790	22115	22486	1247-1247	0.333333	52.5
rpl21-rpl32_p1	rpl21-rpl32	1	AACCCACATTTGCCTTCCAT	GCTGGCTCTTGGGCTTTATA	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.376972	47853	48730	1351-1353	1.000000	86.3
rpl21-rpl32_p2	rpl21-rpl32	2	ACCCACATTTGCCTTCCATT	GCTGGCTCTTGGGCTTTATA	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.376972	47853	48730	1350-1352	1.000000	86.3
rpl21-rpl32_p3	rpl21-rpl32	3	AACCCACATTTGCCTTCCAT	ACACCTGTTTTAGCAGCTGG	20	20	45.0	50.0	58.0	58.4	0.0	17.1	0.419354	47853	48730	1366-1368	1.000000	86.0
rpl21-rpl32_p4	rpl21-rpl32	4	ACCCACATTTGCCTTCCATT	ACACCTGTTTTAGCAGCTGG	20	20	45.0	50.0	58.0	58.4	0.0	17.1	0.419354	47853	48730	1365-1367	1.000000	86.0
rpl21-rpl32_p5	rpl21-rpl32	5	ATTTTCCTGGCTGAACCCAC	GCTGGCTCTTGGGCTTTATA	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.715822	47853	48730	1364-1366	1.000000	83.6
rrn16-ompR_p1	rrn16-ompR	1	GAGCCAGGATCAAACTCTCC	GCACTTACAACTTCGTAGCC	20	20	55.0	50.0	57.7	56.8	0.0	0.0	1.480184	67410	67940	577-597	1.000000	76.8
rrn16-ompR_p2	rrn16-ompR	2	TGCATGTGTTAGGCATACCG	GCACTTACAACTTCGTAGCC	20	20	50.0	50.0	58.3	56.8	0.0	0.0	1.493698	67410	67940	611-631	1.000000	76.7
rrn16-ompR_p3	rrn16-ompR	3	GAGCCAGGATCAAACTCTCC	GCACTTACAACTTCGTAGCCA	20	21	55.0	47.6	57.7	58.6	0.0	0.0	1.913994	67410	67940	577-597	1.000000	73.4
rrn16-ompR_p4	rrn16-ompR	4	GAGCCAGGATCAAACTCTCC	TGCACTTACAACTTCGTAGCC	20	21	55.0	47.6	57.7	58.6	0.0	0.0	1.913994	67410	67940	578-598	1.000000	73.4
rrn16-ompR_p5	rrn16-ompR	5	TGCATGTGTTAGGCATACCG	GCACTTACAACTTCGTAGCCA	20	21	50.0	47.6	58.3	58.6	0.0	0.0	1.927508	67410	67940	611-631	1.000000	73.2
ompR-psbD_p1	ompR-psbD	1	GTTGATGACGAGACCAGCAT	CACCAATTTGACACCATCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034482	68592	68996	1426-1426	0.666667	68.2
ompR-psbD_p2	ompR-psbD	2	GTTGATGACGAGACCAGCAT	TCTGCTGCATCACCATCTTC	20	20	50.0	50.0	58.0	58.0	0.0	1.3	0.049840	68592	68996	1790-1790	0.666667	68.0
ompR-psbD_p3	ompR-psbD	3	GTTGATGACGAGACCAGCAT	AAACCAGCTAGCCTGTCCTA	20	20	50.0	50.0	58.0	58.0	0.0	12.1	0.063114	68592	68996	1605-1605	0.666667	67.9
ompR-psbD_p4	ompR-psbD	4	GTTGATGACGAGACCAGCAT	AACCAGCTAGCCTGTCCTAA	20	20	50.0	50.0	58.0	58.0	0.0	12.1	0.063114	68592	68996	1604-1604	0.666667	67.9
ompR-psbD_p5	ompR-psbD	5	GTTGATGACGAGACCAGCAT	TGTCAAGAAGTTGCAGCCTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.072873	68592	68996	1326-1326	0.666667	67.8
ycf65-groEL_p1	ycf65-groEL	1	CCACGTAATGATGCATGGGA	ATTGTTCCTGGTGGTGGTTC	20	20	50.0	50.0	58.0	58.0	2.5	0.0	0.038689	73336	73953	1172-1173	1.000000	87.8
ycf65-groEL_p2	ycf65-groEL	2	CCACGTAATGATGCATGGGA	GTTGATTTGCACAGATGGGC	20	20	50.0	50.0	58.0	58.0	2.5	0.0	0.034495	73336	73953	1134-1134	0.666667	68.9
ycf65-groEL_p3	ycf65-groEL	3	CCACGTAATGATGCATGGGA	GATTGTTCCTGGTGGTGGTT	20	20	50.0	50.0	58.0	58.0	2.5	0.0	0.038689	73336	73953	1174-1174	0.666667	68.7
ycf65-groEL_p4	ycf65-groEL	4	CCACGTAATGATGCATGGGA	CGGTGGTGTCGCTGTTATAA	20	20	50.0	50.0	58.0	58.0	2.5	0.0	0.035017	73336	73953	1285-1285	0.666667	68.6
ycf65-groEL_p5	ycf65-groEL	5	CCACGTAATGATGCATGGGA	CTTTGTTGTCGCCATTGCAT	20	20	50.0	45.0	58.0	57.9	2.5	0.0	0.102902	73336	73953	1061-1061	0.666667	68.4
rps1_p1	rps1	1	CTTTCCTCGCAGTCACCTAC	TCCATTGCCTTAACCCCTTG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.232460	80728	81345	1294-1302	1.000000	86.1
rps1_p2	rps1	2	GCGGTTTATTTCTGTGGCAC	TCCATTGCCTTAACCCCTTG	20	20	50.0	50.0	58.0	57.8	11.6	0.0	0.252941	80728	81345	1313-1321	1.000000	85.8
rps1_p3	rps1	3	GCGATATAGTTGCAGGCACT	TCCATTGCCTTAACCCCTTG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.359049	80728	81345	1038-1046	1.000000	85.1
rps1_p4	rps1	4	ACCCTTGCCTAGCAGAAGTA	TCCATTGCCTTAACCCCTTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.277508	80728	81345	1340-1340	0.666667	65.3
rps1_p5	rps1	5	AGCCATAAGCCGGATTTTGT	TCCATTGCCTTAACCCCTTG	20	20	45.0	50.0	57.9	57.8	19.3	0.0	0.374005	80728	81345	1594-1594	0.666667	64.7
rpl11-trnW-CCA_p1	rpl11-trnW-CCA	1	GAAAACGGCGCGAATGTTTA	GTCGAGGGTTCAAGTCCTTC	20	20	45.0	55.0	58.0	57.9	12.1	0.0	0.128612	86172	86849	1143-1177	0.666667	70.7
rpl11-trnW-CCA_p2	rpl11-trnW-CCA	2	GAAAACGGCGCGAATGTTTA	CCTATCCCTGAAGCAGCAAG	20	20	45.0	55.0	58.0	58.0	12.1	0.0	0.082334	86172	86849	1493-1529	0.666667	70.5
rpl11-trnW-CCA_p3	rpl11-trnW-CCA	3	TGCTTGTGCCAGTAGAACTG	CCTATCCCTGAAGCAGCAAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.152535	86172	86849	1268-1304	0.666667	70.5
rpl11-trnW-CCA_p4	rpl11-trnW-CCA	4	GAAAACGGCGCGAATGTTTA	GATTCCGATGCACCCTATCC	20	20	45.0	55.0	58.0	57.9	12.1	0.0	0.147491	86172	86849	1542-1542	0.333333	52.2
rpl11-trnW-CCA_p5	rpl11-trnW-CCA	5	GGCGGCGTTTTTAGAACAAA	CCTATCCCTGAAGCAGCAAG	20	20	45.0	55.0	57.9	58.0	9.4	0.0	0.158010	86172	86849	1279-1279	0.333333	52.1
trnS-GCU-trnD-GUC_p1	trnS-GCU-trnD-GUC	1	GCGATACCTTAACGTCTAGACA	TCTTCTCTTTTGTCAAATAAACA	22	23	45.5	26.1	57.5	52.5	22.4	0.0	11.020368	104175	104324	775-775	0.333333	13.3
trnS-GCU-trnD-GUC_p2	trnS-GCU-trnD-GUC	2	TGCGATACCTTAACGTCTAGAC	TCTTCTCTTTTGTCAAATAAACA	22	23	45.5	26.1	57.5	52.5	13.2	0.0	11.020368	104175	104324	776-776	0.333333	13.3
trnS-GCU-trnD-GUC_p3	trnS-GCU-trnD-GUC	3	GCGATACCTTAACGTCTAGACA	TCTTCTCTTTTGTCAAATAAACAA	22	24	45.5	25.0	57.5	53.3	22.4	0.0	11.195410	104175	104324	775-775	0.333333	13.3
trnS-GCU-trnD-GUC_p4	trnS-GCU-trnD-GUC	4	TGCGATACCTTAACGTCTAGAC	TCTTCTCTTTTGTCAAATAAACAA	22	24	45.5	25.0	57.5	53.3	13.2	0.0	11.195410	104175	104324	776-776	0.333333	13.3
trnS-GCU-trnD-GUC_p5	trnS-GCU-trnD-GUC	5	GCGATACCTTAACGTCTAGACA	ATCTTCTCTTTTGTCAAATAAACA	22	24	45.5	25.0	57.5	53.1	22.4	0.0	11.400470	104175	104324	776-776	0.333333	13.3
orf65-ycf45_p1	orf65-ycf45	1	TGCTTGACCACAATCCACAA	AATGCTTCTAGCTCTGTGCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.099964	134678	136161	2832-2832	0.333333	52.5
orf65-ycf45_p2	orf65-ycf45	2	TGCTTGACCACAATCCACAA	GGTACGAGCTGCTAATGCTT	20	20	45.0	50.0	57.9	58.1	0.0	0.2	0.123109	134678	136161	2845-2845	0.333333	52.3
orf65-ycf45_p3	orf65-ycf45	3	TGCTTGACCACAATCCACAA	CCATCAGGAAATCGAGCCTC	20	20	45.0	55.0	57.9	58.1	0.0	1.6	0.188112	134678	136161	2328-2328	0.333333	51.8
orf65-ycf45_p4	orf65-ycf45	4	TGCTTGACCACAATCCACAA	ATGGTACGAGCTGCTAATGC	20	20	45.0	50.0	57.9	58.1	0.0	0.2	0.193353	134678	136161	2847-2847	0.333333	51.8
orf65-ycf45_p5	orf65-ycf45	5	TGCTTGACCACAATCCACAA	TGCTTCTAGCTCTGTGCCTA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.211801	134678	136161	2830-2830	0.333333	51.6
psaD-acsF_p1	psaD-acsF	1	GCAGCTCTTAACCATCCTCC	TGAGCACCGCATCTATCCTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.044207	138834	139668	1408-1459	0.666667	71.2
psaD-acsF_p2	psaD-acsF	2	AGTTGAGCTTGTCATGGCAA	TGAGCACCGCATCTATCCTA	20	20	45.0	50.0	58.0	58.0	13.0	0.0	0.019151	138834	139668	2082-2134	0.666667	71.1
psaD-acsF_p3	psaD-acsF	3	CTCCTGTGCTTCCACCAAAT	TGAGCACCGCATCTATCCTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.089483	138834	139668	1392-1443	0.666667	70.9
psaD-acsF_p4	psaD-acsF	4	ACCAACTCTACCACCTCTCC	TGAGCACCGCATCTATCCTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064644	138834	139668	1850-1902	0.666667	70.8
psaD-acsF_p5	psaD-acsF	5	AGTTGAGCTTGTCATGGCAA	CCCTGTAATGGCTGAATGCT	20	20	45.0	50.0	58.0	57.9	13.0	0.0	0.077497	138834	139668	2313-2365	0.666667	70.6
petA_p1	petA	1	AGCAACCGTCATTCATCCTC	AGGGGCTATTCTTACACCTTC	20	21	50.0	47.6	58.0	56.8	0.0	0.0	2.246094	147988	148962	1721-1841	0.666667	60.7
petA_p2	petA	2	ATCTCGAGAAAGCAACCGTC	AGGGGCTATTCTTACACCTTC	20	21	50.0	47.6	58.0	56.8	20.9	0.0	2.218587	147988	148962	1851-1851	0.333333	35.6
petA_p3	petA	3	ATCTCGAGAAAGCAACCGTC	GGGGCTATTCTTACACCTTCT	20	21	50.0	47.6	58.0	56.8	20.9	0.0	2.218587	147988	148962	1850-1850	0.333333	35.6
petA_p4	petA	4	AGCAACCGTCATTCATCCTC	GGGGCTATTCTTACACCTTCT	20	21	50.0	47.6	58.0	56.8	0.0	0.0	2.246094	147988	148962	1840-1840	0.333333	35.4
petA_p5	petA	5	ATCTCGAGAAAGCAACCGTC	AGGGGCTATTCTTACACCTTCT	20	22	50.0	45.5	58.0	58.3	20.9	0.0	2.265068	147988	148962	1851-1851	0.333333	35.2
petA-tatC_p1	petA-tatC	1	GGTATGGGGCCTACAACAAG	AAATTTTTGGCACCAGCAGC	20	20	55.0	45.0	57.9	58.4	0.8	0.0	0.456179	148963	149551	1407-1407	0.333333	49.7
petA-tatC_p2	petA-tatC	2	GGTATGGGGCCTACAACAAG	CAGCTTGCTCCAGGTGAATA	20	20	55.0	50.0	57.9	57.6	0.8	4.8	0.461053	148963	149551	1605-1605	0.333333	49.6
petA-tatC_p3	petA-tatC	3	GCTTCAAAGACGCTGTTTGG	AAATTTTTGGCACCAGCAGC	20	20	50.0	45.0	58.2	58.4	5.3	0.0	0.644614	148963	149551	1191-1191	0.333333	48.2
petA-tatC_p4	petA-tatC	4	GCTTCAAAGACGCTGTTTGG	CAGCTTGCTCCAGGTGAATA	20	20	50.0	50.0	58.2	57.6	5.3	4.8	0.649488	148963	149551	1389-1389	0.333333	48.1
petA-tatC_p5	petA-tatC	5	TTATCTCCTGGTATGGGGCC	AAATTTTTGGCACCAGCAGC	20	20	55.0	45.0	58.3	58.4	0.0	0.0	0.661089	148963	149551	1416-1416	0.333333	48.0
atpA-sufC_p1	atpA-sufC	1	GAGGCCTATCCAGGAGATGT	AGCCGATTCTGGACTCGATA	20	20	55.0	50.0	58.0	58.0	4.4	11.0	0.040057	176058	177140	1969-2217	0.666667	70.4
atpA-sufC_p2	atpA-sufC	2	AGCAAGCACAAGCATATCGA	AGCCGATTCTGGACTCGATA	20	20	45.0	50.0	58.0	58.0	0.0	11.0	0.045760	176058	177140	2025-2273	0.666667	70.3
atpA-sufC_p3	atpA-sufC	3	TGAAACAGGTCGCAGGAAAA	AGCCGATTCTGGACTCGATA	20	20	45.0	50.0	58.0	58.0	0.0	11.0	0.062901	176058	177140	1695-1943	0.666667	70.2
atpA-sufC_p4	atpA-sufC	4	GAGGCCTATCCAGGAGATGT	CTAAAGTCATCGCTGGGCAT	20	20	55.0	50.0	58.0	58.0	4.4	0.0	0.063676	176058	177140	2351-2599	0.666667	70.2
atpA-sufC_p5	atpA-sufC	5	GAGGCCTATCCAGGAGATGT	TAAAGTCATCGCTGGGCATC	20	20	55.0	50.0	58.0	58.0	4.4	0.0	0.069159	176058	177140	2350-2598	0.666667	70.1
orf12_p1	orf12	1	AATAAGCATCTGTGGCCGAG	CTCTCACACGTGTTGGACAA	20	20	50.0	50.0	58.0	58.1	0.0	20.8	0.106370	191611	192960	2356-2356	0.666667	70.2
orf12_p2	orf12	2	AATAAGCATCTGTGGCCGAG	GGTCAGCATTTTCTTGTGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.111220	191611	192960	2589-2589	0.666667	70.1
orf12_p3	orf12	3	ACTCATGTGCGATCCGTTTT	CTCTCACACGTGTTGGACAA	20	20	45.0	50.0	58.2	58.1	0.0	20.8	0.257001	191611	192960	2320-2320	0.666667	69.0
orf12_p4	orf12	4	AATAAGCATCTGTGGCCGAG	ATTGGCACAGTTCTTGGGAG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.125818	191611	192960	2298-2298	0.333333	52.3
orf12_p5	orf12	5	AATAAGCATCTGTGGCCGAG	CACGTGTTGGACAACCATTG	20	20	50.0	50.0	58.0	57.9	0.0	7.6	0.159187	191611	192960	2350-2350	0.333333	52.1