primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACTGGGGGATTGTTACCTACA	GTGCTTGGGAGTCCTTGAAA	21	20	47.6	50.0	58.1	58.0	0.0	9.6	1.094836	120	1181	1159-1159	1.000000	71.2
psbA_p2	psbA	2	ACTGGGGGATTGTTACCTACA	TGAATACGTGTGCTTGGGAG	21	20	47.6	50.0	58.1	57.9	0.0	0.0	1.169399	120	1181	1168-1168	1.000000	70.6
psbA_p3	psbA	3	ACTGGGGGATTGTTACCTACA	AATACGTGTGCTTGGGAGTC	21	20	47.6	50.0	58.1	57.9	0.0	0.0	1.170855	120	1181	1166-1166	1.000000	70.6
psbA_p4	psbA	4	ACTGGGGGATTGTTACCTACA	GAATACGTGTGCTTGGGAGT	21	20	47.6	50.0	58.1	57.9	0.0	0.0	1.170855	120	1181	1167-1167	1.000000	70.6
psbA_p5	psbA	5	ACTGGGGGATTGTTACCTACA	AGTGAATACGTGTGCTTGGG	21	20	47.6	50.0	58.1	58.2	0.0	0.0	1.267682	120	1181	1170-1170	1.000000	69.9
trnK(UUU)_p1	trnK(UUU)	1	CGGAACTAGTCGGATGGAGT	AGTAGTGGTAGTGGTTCAATTCA	20	23	55.0	39.1	58.6	57.2	0.0	0.0	4.428113	1550	1669	193-193	1.000000	45.0
trnK(UUU)_p2	trnK(UUU)	2	CGGAACTAGTCGGATGGAGT	AGTGGTAGTGGTTCAATTCA	20	20	55.0	40.0	58.6	54.2	0.0	0.0	4.431148	1550	1669	190-190	1.000000	45.0
trnK(UUU)_p3	trnK(UUU)	3	CGGAACTAGTCGGATGGAGT	GGTTCAATTCATTTAGTCGTCCA	20	23	55.0	39.1	58.6	57.1	0.0	0.0	4.482613	1550	1669	181-181	1.000000	44.6
trnK(UUU)_p4	trnK(UUU)	4	CGGAACTAGTCGGATGGAGT	TGGTTCAATTCATTTAGTCGTCC	20	23	55.0	39.1	58.6	57.1	0.0	0.0	4.482613	1550	1669	182-182	1.000000	44.6
trnK(UUU)_p5	trnK(UUU)	5	ACTCGAACCCGGAACTAGTC	AGTAGTGGTAGTGGTTCAATTCA	20	23	55.0	39.1	58.8	57.2	0.7	0.0	4.646503	1550	1669	202-202	1.000000	43.2
matK-trnK(UUU)_p1	matK-trnK(UUU)	1	TCTTGCAGGAGAAGCGGATA	GGTCCAGTTGATCAGTGCAA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.618656	3468	4791	1512-1558	1.000000	75.4
matK-trnK(UUU)_p2	matK-trnK(UUU)	2	TCTTGCAGGAGAAGCGGATA	TTGGTCCAGTTGATCAGTGC	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.618656	3468	4791	1514-1560	1.000000	75.4
matK-trnK(UUU)_p3	matK-trnK(UUU)	3	CTTGCAGGAGAAGCGGATAT	GGTCCAGTTGATCAGTGCAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.644045	3468	4791	1511-1557	1.000000	75.2
matK-trnK(UUU)_p4	matK-trnK(UUU)	4	CTTGCAGGAGAAGCGGATAT	TTGGTCCAGTTGATCAGTGC	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.644045	3468	4791	1513-1559	1.000000	75.2
matK-trnK(UUU)_p5	matK-trnK(UUU)	5	TCTTGCAGGAGAAGCGGATA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.5	58.2	0.0	17.1	0.683296	3468	4791	1433-1478	1.000000	74.9
psbD-trnT(GGU)_p1	psbD-trnT(GGU)	1	AGGAAAGAGCAATATGCCGG	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	0.0	7.6	0.091195	21367	22301	1113-1113	1.000000	79.3
psbD-trnT(GGU)_p2	psbD-trnT(GGU)	2	GGAAAGAGCAATATGCCGGA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	5.0	7.6	0.096749	21367	22301	1112-1112	1.000000	79.3
psbD-trnT(GGU)_p3	psbD-trnT(GGU)	3	AGGAAAGAGCAATATGCCGG	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113350	21367	22301	1116-1116	1.000000	79.2
psbD-trnT(GGU)_p4	psbD-trnT(GGU)	4	GGAAAGAGCAATATGCCGGA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.0	58.1	5.0	0.0	0.118904	21367	22301	1115-1115	1.000000	79.1
psbD-trnT(GGU)_p5	psbD-trnT(GGU)	5	GGACCAGCCTACAAAAACGA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.1	58.1	0.0	7.6	0.177181	21367	22301	1095-1095	1.000000	78.7
rpoC2_p1	rpoC2	1	GAACTCTTGGCACGTTGTTG	TTTCAATTGAAGTGCTTTAAGCA	20	23	50.0	30.4	57.9	55.9	9.0	16.3	5.252838	36600	36719	299-299	1.000000	40.3
rpoC2_p2	rpoC2	2	AACATCAGGGAACTCTTGGC	TTTCAATTGAAGTGCTTTAAGCA	20	23	50.0	30.4	57.8	55.9	0.0	16.3	5.354091	36600	36719	308-308	1.000000	40.3
rpoC2_p3	rpoC2	3	AACTCTTGGCACGTTGTTGA	TTTCAATTGAAGTGCTTTAAGCA	20	23	45.0	30.4	58.3	55.9	0.0	16.3	5.402283	36600	36719	298-298	1.000000	40.3
rpoC2_p4	rpoC2	4	ACTCTTGGCACGTTGTTGAA	TTTCAATTGAAGTGCTTTAAGCA	20	23	45.0	30.4	58.3	55.9	0.0	16.3	5.402283	36600	36719	297-297	1.000000	40.3
rpoC2_p5	rpoC2	5	CTTGGCACGTTGTTGAATCG	TTTCAATTGAAGTGCTTTAAGCA	20	23	50.0	30.4	58.3	55.9	0.0	16.3	5.473755	36600	36719	294-294	1.000000	40.3
rps4_p1	rps4	1	TACTTCAGCGAAAGGAGTGC	TGCTTTGTGGATACTGTAGCT	20	21	50.0	42.9	57.9	57.0	6.2	2.8	2.061357	46368	46973	743-743	1.000000	63.6
rps4_p2	rps4	2	TCAGCGAAAGGAGTGCAAAT	TGCTTTGTGGATACTGTAGCT	20	21	45.0	42.9	58.1	57.0	0.0	2.8	2.084852	46368	46973	739-739	1.000000	63.4
rps4_p3	rps4	3	TACTTCAGCGAAAGGAGTGC	TGTGGATACTGTAGCTCAGAGT	20	22	50.0	45.5	57.9	58.0	6.2	16.8	2.110733	46368	46973	738-738	1.000000	63.2
rps4_p4	rps4	4	TCAGCGAAAGGAGTGCAAAT	TGTGGATACTGTAGCTCAGAGT	20	22	45.0	45.5	58.1	58.0	0.0	16.8	2.134228	46368	46973	734-734	1.000000	63.0
rps4_p5	rps4	5	TACTTCAGCGAAAGGAGTGC	GCTTTGTGGATACTGTAGCTCA	20	22	50.0	45.5	57.9	58.1	6.2	2.8	2.218446	46368	46973	742-742	1.000000	62.4
trnL(UAA)_p1	trnL(UAA)	1	CTGGATCCTTGTCTCAAACCA	TCCAATGGTCTTTTTCTTGGGA	21	22	47.6	40.9	57.6	57.8	14.2	0.0	3.615344	48392	49143	798-802	1.000000	51.5
trnL(UAA)_p2	trnL(UAA)	2	ACTGGATCCTTGTCTCAAACC	TCCAATGGTCTTTTTCTTGGGA	21	22	47.6	40.9	57.6	57.8	14.2	0.0	3.616966	48392	49143	799-803	1.000000	51.5
trnL(UAA)_p3	trnL(UAA)	3	CTGGATCCTTGTCTCAAACCA	GTCCAATGGTCTTTTTCTTGGG	21	22	47.6	45.5	57.6	57.7	14.2	0.0	3.680807	48392	49143	799-803	1.000000	51.0
trnL(UAA)_p4	trnL(UAA)	4	ACTGGATCCTTGTCTCAAACC	GTCCAATGGTCTTTTTCTTGGG	21	22	47.6	45.5	57.6	57.7	14.2	0.0	3.682429	48392	49143	800-804	1.000000	50.9
trnL(UAA)_p5	trnL(UAA)	5	TGGATCCTTGTCTCAAACCA	TCCAATGGTCTTTTTCTTGGGA	20	22	45.0	40.9	56.4	57.8	14.2	0.0	3.833271	48392	49143	797-801	1.000000	49.7
rbcL-accD_p1	rbcL-accD	1	CTAGCAAATGGAGCCCTGAG	CCCGCTAGCATCAAGACTAC	20	20	55.0	55.0	58.0	57.9	0.0	16.4	0.176983	58248	59122	1063-1063	1.000000	78.7
rbcL-accD_p2	rbcL-accD	2	CTAGCAAATGGAGCCCTGAG	CCGCTAGCATCAAGACTACC	20	20	55.0	55.0	58.0	57.9	0.0	16.4	0.176983	58248	59122	1062-1062	1.000000	78.7
rbcL-accD_p3	rbcL-accD	3	CTAGCAAATGGAGCCCTGAG	CGCTAGCATCAAGACTACCC	20	20	55.0	55.0	58.0	57.9	0.0	16.4	0.176983	58248	59122	1061-1061	1.000000	78.7
rbcL-accD_p4	rbcL-accD	4	TGAAGCTAGCAAATGGAGCC	CCGCTAGCATCAAGACTACC	20	20	50.0	55.0	58.2	57.9	18.8	16.4	0.386356	58248	59122	1067-1067	1.000000	77.0
rbcL-accD_p5	rbcL-accD	5	TGAAGCTAGCAAATGGAGCC	CGCTAGCATCAAGACTACCC	20	20	50.0	55.0	58.2	57.9	18.8	16.4	0.386356	58248	59122	1066-1066	1.000000	77.0
accD_p1	accD	1	TTAGGGGACAGAAAGGCTCT	ACGTGCTACAACTACAAAGAAGT	20	23	50.0	39.1	57.7	58.3	0.0	0.0	3.653971	59123	60763	1718-1739	1.000000	51.7
accD_p2	accD	2	TTAGGGGACAGAAAGGCTCT	ACGTGCTACAACTACAAAGAAG	20	22	50.0	40.9	57.7	56.7	0.0	0.0	3.658751	59123	60763	1718-1739	1.000000	51.7
accD_p3	accD	3	TTAGGGGACAGAAAGGCTCT	CGTGCTACAACTACAAAGAAGT	20	22	50.0	40.9	57.7	56.7	0.0	0.0	3.658751	59123	60763	1717-1738	1.000000	51.7
accD_p4	accD	4	TTAGGGGACAGAAAGGCTCT	ACGTGCTACAACTACAAAGA	20	20	50.0	40.0	57.7	54.7	0.0	0.0	3.664585	59123	60763	1718-1739	1.000000	51.7
accD_p5	accD	5	TTAGGGGACAGAAAGGCTCT	ACGTGCTACAACTACAAAGAA	20	21	50.0	38.1	57.7	55.5	0.0	0.0	3.839450	59123	60763	1718-1739	1.000000	50.3
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATGTATCCTCGT	AATCTCCTGCTCTTCGAGGA	20	20	50.0	50.0	57.4	57.8	8.2	0.0	0.714763	70861	71673	903-903	1.000000	74.4
rpl20-rps12_p2	rpl20-rps12	2	TCCGAGCTATGTATCCTCGT	ACGAAATCTCCTGCTCTTCG	20	20	50.0	50.0	57.4	57.7	8.2	0.0	0.849087	70861	71673	907-907	1.000000	73.3
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATGTATCCTCGT	CGCCATGCCAACCATTAAAC	20	20	50.0	50.0	57.4	58.4	8.2	0.0	0.923981	70861	71673	965-965	1.000000	72.7
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATGTATCCTCGT	TGTCACGAAATCTCCTGCTC	20	20	50.0	50.0	57.4	57.6	8.2	0.0	0.933542	70861	71673	911-911	1.000000	72.6
rpl20-rps12_p5	rpl20-rps12	5	TCCGAGCTATGTATCCTCGT	ATCTCCTGCTCTTCGAGGAT	20	20	50.0	50.0	57.4	57.6	8.2	0.0	0.943325	70861	71673	902-902	1.000000	72.5
petD_p1	petD	1	TGGATTATTTCAAAAATAAGGCATGT	AGGGAAGATCCAGAAAAGGA	26	20	26.9	45.0	56.4	55.4	9.8	0.0	10.143086	79641	81100	1631-1631	1.000000	40.0
petD_p2	petD	2	AGTCTATTTTTCTAAAAGAGGATGGA	AGGGAAGATCCAGAAAAGGA	26	20	30.8	45.0	56.1	55.4	6.7	0.0	10.459640	79641	81100	1544-1544	1.000000	40.0
petD_p3	petD	3	TGGATTATTTCAAAAATAAGGCATGTT	AGGGAAGATCCAGAAAAGGA	27	20	25.9	45.0	57.0	55.4	9.8	0.0	10.549210	79641	81100	1631-1631	1.000000	40.0
petD_p4	petD	4	TGGATTATTTCAAAAATAAGGCATGT	TCTAGGGAAGATCCAGAAAAGGA	26	23	26.9	43.5	56.4	58.0	9.8	0.0	10.590184	79641	81100	1634-1634	1.000000	40.0
petD_p5	petD	5	TGGATTATTTCAAAAATAAGGCATG	AGGGAAGATCCAGAAAAGGA	25	20	28.0	45.0	54.9	55.4	9.8	0.0	10.664474	79641	81100	1631-1631	1.000000	40.0
rpoA_p1	rpoA	1	ACCGAAGATTCCAAACTCAT	AGACGTGTGTAAAATTCAAGAGA	20	23	40.0	34.8	54.7	56.2	0.0	0.0	8.178379	81303	82322	1222-1223	1.000000	40.1
rpoA_p2	rpoA	2	TCATACCGAAGATTCCAAACTCA	AGACGTGTGTAAAATTCAAGAGA	23	23	39.1	34.8	57.6	56.2	0.0	0.0	8.243325	81303	82322	1226-1227	1.000000	40.1
rpoA_p3	rpoA	3	ACCGAAGATTCCAAACTCATT	AGACGTGTGTAAAATTCAAGAGA	21	23	38.1	34.8	55.5	56.2	0.0	0.0	8.337270	81303	82322	1222-1223	1.000000	40.1
rpoA_p4	rpoA	4	ACCGAAGATTCCAAACTCAT	ACGTGTGTAAAATTCAAGAGATTCC	20	25	40.0	36.0	54.7	58.1	0.0	0.0	8.450729	81303	82322	1220-1221	1.000000	40.1
rpoA_p5	rpoA	5	ACCGAAGATTCCAAACTCAT	CGTGTGTAAAATTCAAGAGATTCCA	20	25	40.0	36.0	54.7	58.1	0.0	0.0	8.451803	81303	82322	1219-1220	1.000000	40.1
ndhA_p1	ndhA	1	ATGGAATCTGTGGCGTTAGC	TGAAGTCAAATAAATTGCCATCCA	20	24	50.0	33.3	58.3	57.4	0.0	0.0	4.933991	119689	120284	678-702	1.000000	43.7
ndhA_p2	ndhA	2	GCGTTAGCCCATGGGATTTT	TGAAGTCAAATAAATTGCCATCCA	20	24	50.0	33.3	58.9	57.4	36.0	0.0	5.491133	119689	120284	666-690	1.000000	43.2
ndhA_p3	ndhA	3	ATGGAATCTGTGGCGTTAGC	AGTCAAATAAATTGCCATCCAAGA	20	24	50.0	33.3	58.3	57.1	0.0	0.0	5.187343	119689	120284	675-699	1.000000	43.1
ndhA_p4	ndhA	4	ATGGAATCTGTGGCGTTAGC	TGAAGTCAAATAAATTGCCATCCAA	20	25	50.0	32.0	58.3	58.0	0.0	0.0	5.333831	119689	120284	678-702	1.000000	43.1
ndhA_p5	ndhA	5	ATGGAATCTGTGGCGTTAGC	TTGAAGTCAAATAAATTGCCATCCA	20	25	50.0	32.0	58.3	58.0	0.0	0.0	5.333831	119689	120284	679-703	1.000000	43.1
ndhD-ccsA_p1	ndhD-ccsA	1	TTCTCGATGGCTTCTCTTGC	GTCCAAGGCACAAATTCAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.085997	122126	122438	622-629	1.000000	80.3
ndhD-ccsA_p2	ndhD-ccsA	2	CTCGATGGCTTCTCTTGCAT	GTCCAAGGCACAAATTCAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119434	122126	122438	620-627	1.000000	80.1
ndhD-ccsA_p3	ndhD-ccsA	3	TTCTCGATGGCTTCTCTTGC	TTTTACCCAAGGTTTCGCCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.084570	122126	122438	1100-1107	1.000000	79.9
ndhD-ccsA_p4	ndhD-ccsA	4	TTCTCGATGGCTTCTCTTGC	AGGCATTCTTTCTGGAGCAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.141509	122126	122438	761-768	1.000000	79.7
ndhD-ccsA_p5	ndhD-ccsA	5	CTCGATGGCTTCTCTTGCAT	TTTTACCCAAGGTTTCGCCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.118006	122126	122438	1098-1105	1.000000	79.6
ccsA_p1	ccsA	1	GTCTGTCAGACGATGTTTTT	CGAGTGGCGGCATACAAATA	20	20	40.0	50.0	53.9	58.4	17.0	17.2	4.523344	122439	123371	1089-1089	1.000000	43.9
ccsA_p2	ccsA	2	GTCTGTCAGACGATGTTTTT	TGGCGGCATACAAATAGACA	20	20	40.0	45.0	53.9	57.3	17.0	8.0	4.801535	122439	123371	1085-1085	1.000000	41.7
ccsA_p3	ccsA	3	TCAAACGTTTTTCAAAAACTCGT	CGAGTGGCGGCATACAAATA	23	20	30.4	50.0	56.6	58.4	20.8	17.2	4.810682	122439	123371	1131-1131	1.000000	41.6
ccsA_p4	ccsA	4	GTCTGTCAGACGATGTTTTTATGT	CGAGTGGCGGCATACAAATA	24	20	37.5	50.0	57.5	58.4	17.0	17.2	4.890254	122439	123371	1089-1089	1.000000	41.0
ccsA_p5	ccsA	5	ACGTTTTTCAAAAACTCGTACA	CGAGTGGCGGCATACAAATA	22	20	31.8	50.0	55.4	58.4	20.8	17.2	5.024774	122439	123371	1127-1127	1.000000	40.1
rpl32-ndhA_p1	rpl32-ndhA	1	CACAATGCATGGGAACGAAA	GCGTGATTGGTTGGAACATG	20	20	45.0	50.0	57.3	58.0	5.9	0.0	0.740020	124417	125517	1631-1657	1.000000	75.3
rpl32-ndhA_p2	rpl32-ndhA	2	CACAATGCATGGGAACGAAA	GCAATATCTCTCCGCGTGAT	20	20	45.0	50.0	57.3	57.9	5.9	4.9	0.792962	124417	125517	1644-1670	1.000000	74.9
rpl32-ndhA_p3	rpl32-ndhA	3	CACAATGCATGGGAACGAAA	CAAGTCTTGTCCCCATTGCT	20	20	45.0	50.0	57.3	58.1	5.9	4.3	0.806133	124417	125517	1772-1798	1.000000	74.7
rpl32-ndhA_p4	rpl32-ndhA	4	CACAATGCATGGGAACGAAA	TCAAGTCTTGTCCCCATTGC	20	20	45.0	50.0	57.3	58.1	5.9	12.3	0.811574	124417	125517	1773-1799	1.000000	74.7
rpl32-ndhA_p5	rpl32-ndhA	5	CACAATGCATGGGAACGAAA	TGGGAAGTCCAATGGCAAAA	20	20	45.0	45.0	57.3	57.9	5.9	0.0	0.815374	124417	125517	2195-2221	1.000000	74.4