primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	rpoC1	1	GGAAGGCTTCCTCGATCTCT	AACCAGACACTACTCCCGAA	20	20	55.0	50.0	58.6	58.0	33.9	0.0	0.596401	11752	14253	2667-2810	1.000000	82.9
rpoC1_p2	rpoC1	2	GGAAGGCTTCCTCGATCTCT	AGTTTGGGCTCTAGAAGGGT	20	20	55.0	50.0	58.6	58.0	33.9	0.0	0.637311	11752	14253	2798-2941	1.000000	82.4
rpoC1_p3	rpoC1	3	GGAAGGCTTCCTCGATCTCT	AGGAAGATCCAGACGAGGAG	20	20	55.0	55.0	58.6	57.9	33.9	0.0	0.656929	11752	14253	2840-2983	1.000000	82.2
rpoC1_p4	rpoC1	4	GGAAGGCTTCCTCGATCTCT	GCGCCATTCTTACTGGAGAA	20	20	55.0	50.0	58.6	58.0	33.9	0.0	0.615206	11752	14253	2703-2846	0.833333	74.5
rpoC1_p5	rpoC1	5	GGAAGGCTTCCTCGATCTCT	GGTTACGCAACAGCCTCTTA	20	20	55.0	50.0	58.6	57.9	33.9	0.1	0.679980	11752	14253	2861-2993	0.666667	67.8
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	AGGACTGAAAATCCTCGTGC	TTGGCCTTTACGCAAAGACT	20	20	50.0	45.0	57.9	58.0	0.0	9.8	0.124945	37866	38379	616-632	1.000000	84.4
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AGGACTGAAAATCCTCGTGC	GTTGGCCTTTACGCAAAGAC	20	20	50.0	50.0	57.9	58.0	0.0	9.8	0.146887	37866	38379	617-633	1.000000	84.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AGGACTGAAAATCCTCGTGC	TGAGAGTTGGATTGGTTGGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.190905	37866	38379	631-647	1.000000	83.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AGGACTGAAAATCCTCGTGC	AGTTGGATTGGTTGGCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.202244	37866	38379	627-643	1.000000	83.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GACTGAAAATCCTCGTGCCA	TTGGCCTTTACGCAAAGACT	20	20	50.0	45.0	58.2	58.0	0.0	9.8	0.215930	37866	38379	614-630	1.000000	83.7
psbT-psbB_p1	psbT-psbB	1	CAATGGAGTAGTGCCCCATC	GGGGATGGAATAGCTGTTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.040246	71781	71974	1244-1256	1.000000	83.3
psbT-psbB_p2	psbT-psbB	2	CATCAATGGAGTAGTGCCCC	GGGGATGGAATAGCTGTTGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.040246	71781	71974	1247-1259	1.000000	83.3
psbT-psbB_p3	psbT-psbB	3	CGAGCGGATGTTCTGGTTTA	GGGGATGGAATAGCTGTTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.017700	71781	71974	1367-1379	1.000000	83.1
psbT-psbB_p4	psbT-psbB	4	GCACGAGGACTAGTGGAAAG	GGGGATGGAATAGCTGTTGG	20	20	55.0	55.0	58.0	58.0	13.2	0.0	0.015449	71781	71974	914-923	0.833333	76.7
psbT-psbB_p5	psbT-psbB	5	GCACGAGGACTAGTGGAAAG	TGTACGTCGTATGCCTACCT	20	20	55.0	50.0	58.0	58.0	13.2	8.6	0.045017	71781	71974	846-855	0.833333	76.6
psbB-clpP_p1	psbB-clpP	1	CCCAACCGGAAACTAAAGCT	GTCCAAGTACTAGCGGGTTG	20	20	50.0	55.0	58.1	58.0	9.5	0.0	0.100333	73502	74280	1258-1277	1.000000	88.5
psbB-clpP_p2	psbB-clpP	2	AGCCCAACCGGAAACTAAAG	GTCCAAGTACTAGCGGGTTG	20	20	50.0	55.0	58.1	58.0	9.5	0.0	0.100333	73502	74280	1260-1279	1.000000	88.4
psbB-clpP_p3	psbB-clpP	3	AGGACGAACACTGAGATGGA	GTCCAAGTACTAGCGGGTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.081239	73502	74280	1827-1840	0.833333	78.1
psbB-clpP_p4	psbB-clpP	4	AGGACGAACACTGAGATGGA	AGGACCCTGTTTCCGTTTTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098213	73502	74280	2068-2081	0.666667	69.9
psbB-clpP_p5	psbB-clpP	5	AGGACGAACACTGAGATGGA	GGACCCTGTTTCCGTTTTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098213	73502	74280	2067-2080	0.666667	69.9
clpP_p1	clpP	1	AGGGTGTTGAAAGACTTGGC	CAGCGGTAATGTGGAAAGGA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.415539	74281	75069	1268-1577	1.000000	88.7
clpP_p2	clpP	2	AGGGTGTTGAAAGACTTGGC	AGCCTTTTTCTTGCCCAAGA	20	20	50.0	45.0	58.3	57.9	0.0	6.6	0.394200	74281	75069	1368-1677	1.000000	88.6
clpP_p3	clpP	3	AGGGTGTTGAAAGACTTGGC	TTAGAGTACCGCCCTGTCAA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.380102	74281	75069	979-991	0.833333	78.3
clpP_p4	clpP	4	AGGGTGTTGAAAGACTTGGC	TAACCACGCGAATACCAAGG	20	20	50.0	50.0	58.3	58.0	0.0	15.9	0.320526	74281	75069	1503-1514	0.333333	51.1
clpP_p5	clpP	5	AGGGTGTTGAAAGACTTGGC	GCGAATACCAAGGGCCTAAA	20	20	50.0	50.0	58.3	58.0	0.0	6.4	0.354591	74281	75069	1496-1507	0.333333	50.8
clpP-ycf1_p1	clpP-ycf1	1	CAACCCGCTAGTACTTGGAC	GTGCAGCGATAAAAAGCGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.042224	75070	75608	998-1295	1.000000	90.5
clpP-ycf1_p2	clpP-ycf1	2	CAACCCGCTAGTACTTGGAC	CAGCGATAAAAAGCGTTGCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.043559	75070	75608	995-1292	1.000000	90.5
clpP-ycf1_p3	clpP-ycf1	3	CAACCCGCTAGTACTTGGAC	TAACCACGCGAATACCAAGG	20	20	55.0	50.0	58.0	58.0	0.0	15.9	0.018907	75070	75608	989-1000	0.333333	53.7
clpP-ycf1_p4	clpP-ycf1	4	AGAAAACGGAAACAGGGTCC	TAACCACGCGAATACCAAGG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.035881	75070	75608	749-760	0.333333	53.7
clpP-ycf1_p5	clpP-ycf1	5	GAAAACGGAAACAGGGTCCT	TAACCACGCGAATACCAAGG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.035881	75070	75608	748-759	0.333333	53.7
ndhI-ndhG_p1	ndhI-ndhG	1	CAACAAGCAATACAGGCTGC	TGAGACCTAGCGTTAGAGGG	20	20	50.0	55.0	58.0	57.9	15.9	8.0	0.067534	88228	88534	851-853	1.000000	87.5
ndhI-ndhG_p2	ndhI-ndhG	2	CAACAAGCAATACAGGCTGC	TGCTATCTCCTACGGTCCAG	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.030642	88228	88534	1088-1090	1.000000	86.3
ndhI-ndhG_p3	ndhI-ndhG	3	CCTATCGCTTTGGGCAATCT	TGCTATCTCCTACGGTCCAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044468	88228	88534	1295-1297	1.000000	85.6
ndhI-ndhG_p4	ndhI-ndhG	4	CACGGCGACTGGGTTTATAA	TGCTATCTCCTACGGTCCAG	20	20	50.0	55.0	58.0	58.0	16.6	0.0	0.030277	88228	88534	1116-1116	0.833333	74.5
ndhI-ndhG_p5	ndhI-ndhG	5	CACGGCGACTGGGTTTATAA	TGAGACCTAGCGTTAGAGGG	20	20	50.0	55.0	58.0	57.9	16.6	8.0	0.067169	88228	88534	879-879	0.833333	74.3
rpl32-ndhF_p1	rpl32-ndhF	1	CTTTTACTGCGGCTCGGTAT	CCTATTGCCGCAGAAGCTAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.110509	94065	94807	896-902	1.000000	84.9
rpl32-ndhF_p2	rpl32-ndhF	2	CGGTATGCTTTTCCCTTCCA	CCTATTGCCGCAGAAGCTAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.169224	94065	94807	882-888	1.000000	84.5
rpl32-ndhF_p3	rpl32-ndhF	3	GCTCGGTATGCTTTTCCCTT	CCTATTGCCGCAGAAGCTAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.295004	94065	94807	885-891	1.000000	83.5
rpl32-ndhF_p4	rpl32-ndhF	4	CTTTTACTGCGGCTCGGTAT	TAAAAGCGGAAGGAAAGGCA	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.344002	94065	94807	879-885	1.000000	83.1
rpl32-ndhF_p5	rpl32-ndhF	5	TTTTACTGCGGCTCGGTATG	CCTATTGCCGCAGAAGCTAA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.399095	94065	94807	895-901	1.000000	82.6
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	GGCTGTTAACCGATTAGTCGT	GTTCAAATCCCTCCTGGCTC	21	20	47.6	55.0	58.1	58.2	6.9	0.0	1.358743	97633	97717	146-151	0.833333	71.0
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	GGCTGTTAACCGATTAGTCGT	AGAGGATTAGAGCACGTGGT	21	20	47.6	50.0	58.1	58.1	6.9	22.1	1.261210	97633	97717	186-191	0.833333	70.0
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	GGCTGTTAACCGATTAGTCGT	CAGAGGATTAGAGCACGTGG	21	20	47.6	55.0	58.1	57.8	6.9	18.9	1.331080	97633	97717	187-192	0.833333	69.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	GGCTGTTAACCGATTAGTCGT	GCTGAGAGCGGATCAAGTAG	21	20	47.6	55.0	58.1	57.9	6.9	0.0	1.247913	97633	97717	224-229	0.833333	69.0
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	GGCTGTTAACCGATTAGTCGT	GAGCGGATCAAGTAGAGACG	21	20	47.6	55.0	58.1	57.6	6.9	0.0	1.508106	97633	97717	219-224	0.833333	67.0
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	103628	104257	722-738	1.000000	83.4
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	103628	104257	773-789	1.000000	83.1
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.496177	103628	104257	744-760	1.000000	83.1
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	57.8	58.3	4.7	0.0	0.476037	103628	104257	795-811	1.000000	82.8
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	57.8	57.6	4.7	0.0	0.528602	103628	104257	782-798	1.000000	82.5
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	CATTGCGGAAAATTCCCCAC	TCCGAAAATTCTTCACCGCA	20	20	50.0	45.0	58.0	58.1	6.6	0.0	0.123192	105743	107263	2312-2377	1.000000	90.8
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	ATTCCGGATAACGCTTGCAT	TCCGAAAATTCTTCACCGCA	20	20	45.0	45.0	58.0	58.1	4.1	0.0	0.147121	105743	107263	2474-2539	1.000000	90.1
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	TGCCCCTACCATACTCTAGC	TCCGAAAATTCTTCACCGCA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.125562	105743	107263	2582-2647	1.000000	90.0
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	TCGTCTCTCAGTGTCAGTGT	TCCGAAAATTCTTCACCGCA	20	20	50.0	45.0	58.0	58.1	7.5	0.0	0.145736	105743	107263	2679-2744	1.000000	89.7
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	CGTAGCCTTGGTAAGCCATT	TCCGAAAATTCTTCACCGCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.152827	105743	107263	2217-2240	0.833333	83.2
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	TCGTTGAACCGATGACCATC	TCGAACCTGTTCCGAAAAGG	20	20	50.0	50.0	58.0	58.1	0.0	15.0	0.130885	107336	108265	2057-2123	0.666667	68.2
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	TCGTTGAACCGATGACCATC	TTTGACTCGAACCTGTTCCG	20	20	50.0	50.0	58.0	58.1	0.0	4.5	0.143907	107336	108265	2063-2129	0.666667	68.1
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	TCGTTGAACCGATGACCATC	GACTCGAACCTGTTCCGAAA	20	20	50.0	50.0	58.0	57.9	0.0	4.5	0.149064	107336	108265	2060-2126	0.666667	68.1
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	TCGTTGAACCGATGACCATC	CACTAGAACGCCCTTGTTGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.168401	107336	108265	1781-1848	0.666667	68.1
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	TCGTTGAACCGATGACCATC	ACTAGAACGCCCTTGTTGAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.169901	107336	108265	1780-1847	0.666667	68.1
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GCGTTTGTCTCGGGTCAT	CCGCGACTTCCCAATTATGA	18	20	55.6	50.0	57.7	58.1	0.0	8.2	2.330852	119789	120217	469-602	1.000000	73.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	GCGTTTGTCTCGGGTCAT	GGGTGCTTTAACCATTCAGC	18	20	55.6	50.0	57.7	57.3	0.0	0.0	2.933706	119789	120217	446-579	1.000000	68.5
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	GCGTTTGTCTCGGGTCAT	GGTGCTTTAACCATTCAGCC	18	20	55.6	50.0	57.7	57.3	0.0	0.0	2.933706	119789	120217	445-578	1.000000	68.5
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	GCGTTTGTCTCGGGTCAT	TGAGTTGGGTGCTTTAACCA	18	20	55.6	45.0	57.7	57.3	0.0	0.0	3.017543	119789	120217	452-585	1.000000	67.9
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	GCGTTTGTCTCGGGTCAT	ATTGGAAGACCTGCATCCAG	18	20	55.6	50.0	57.7	57.6	0.0	6.1	2.710910	119789	120217	512-634	0.833333	61.4
chlL-trnH-GUG_p1	chlL-trnH-GUG	1	CCGATTCCGCCTTTTCCATA	CAAGTGGTTAAGGCAGTGGA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.308559	122894	123635	839-857	1.000000	83.8
chlL-trnH-GUG_p2	chlL-trnH-GUG	2	GGATTTACCGATTCCGCCTT	CAAGTGGTTAAGGCAGTGGA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.307180	122894	123635	846-864	1.000000	83.7
chlL-trnH-GUG_p3	chlL-trnH-GUG	3	ACGTCTGGCTAGAGCAATTG	CAAGTGGTTAAGGCAGTGGA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.286088	122894	123635	885-903	1.000000	83.6
chlL-trnH-GUG_p4	chlL-trnH-GUG	4	CACGTCTGGCTAGAGCAATT	CAAGTGGTTAAGGCAGTGGA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.286088	122894	123635	886-904	1.000000	83.6
chlL-trnH-GUG_p5	chlL-trnH-GUG	5	AACTGGTCGTGGATTTACCG	CAAGTGGTTAAGGCAGTGGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.359302	122894	123635	856-874	1.000000	83.2
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GCTAGTGATCCAGTCGCAAA	TAATCCACTCCATCCGACGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.071654	125397	125888	602-613	1.000000	84.8
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTGATCCAGTCGCAAAAACG	TAATCCACTCCATCCGACGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.111038	125397	125888	598-609	1.000000	84.5
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TGATCCAGTCGCAAAAACGA	TAATCCACTCCATCCGACGA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.189121	125397	125888	597-608	1.000000	83.9
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GCTAGTGATCCAGTCGCAAA	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.142885	125397	125888	673-684	1.000000	83.7
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	GTGATCCAGTCGCAAAAACG	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.182268	125397	125888	669-680	1.000000	83.4
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AGTCGCACTTAAAAGCCGAG	GCTAAAGAAGCGTTTGGTACC	20	21	50.0	47.6	58.6	57.5	6.4	4.4	2.037249	128380	129946	1636-2047	1.000000	75.7
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	TAGTCGCACTTAAAAGCCGA	GCTAAAGAAGCGTTTGGTACC	20	21	45.0	47.6	57.3	57.5	3.0	4.4	2.195402	128380	129946	1637-2048	1.000000	74.4
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TCGCACTTAAAAGCCGAGTA	GCTAAAGAAGCGTTTGGTACC	20	21	45.0	47.6	57.3	57.5	3.0	4.4	2.195402	128380	129946	1634-2045	1.000000	74.4
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	CGCACTTAAAAGCCGAGTAC	GCTAAAGAAGCGTTTGGTACC	20	21	50.0	47.6	57.2	57.5	0.0	4.4	2.248910	128380	129946	1633-2044	1.000000	74.0
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	GTCGCACTTAAAAGCCGAGT	GCTAAAGAAGCGTTTGGTACC	20	21	50.0	47.6	58.9	57.5	6.4	4.4	2.320909	128380	129946	1635-2046	1.000000	73.4