primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 20 20 55.0 55.0 58.1 57.9 0.0 13.4 0.194818 77 539 505-530 1.000000 83.4 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.199180 77 539 519-544 1.000000 83.3 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.200688 77 539 518-543 1.000000 83.3 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.203648 77 539 508-533 1.000000 83.3 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.200688 77 539 520-545 1.000000 83.2 trnK-UUU_p1 trnK-UUU 1 TGCTAGTGTCAACCAATATCGA AACAAGGGGATCGGTCTAGA 22 20 40.9 50.0 57.3 57.5 0.0 0.0 3.274071 1819 4370 2989-2989 1.000000 54.4 trnK-UUU_p2 trnK-UUU 2 TGCTAGTGTCAACCAATATCGA ACAAGGGGATCGGTCTAGAA 22 20 40.9 50.0 57.3 57.5 0.0 0.0 3.274071 1819 4370 2988-2988 1.000000 54.4 trnK-UUU_p3 trnK-UUU 3 CCAAGCGCACACATTCTCTA GCTCAATGGTTTAATTGCTTCG 20 22 50.0 40.9 58.3 56.9 11.5 0.0 3.360057 1819 4370 2890-2890 1.000000 53.7 trnK-UUU_p4 trnK-UUU 4 CCAAGCGCACACATTCTCTA AGCTCAATGGTTTAATTGCTTCG 20 23 50.0 39.1 58.3 58.3 11.5 0.0 3.537306 1819 4370 2891-2891 1.000000 52.3 trnK-UUU_p5 trnK-UUU 5 TGCTAGTGTCAACCAATATCGA GGAGATAACAAGGGGATCGG 22 20 40.9 55.0 57.3 57.2 0.0 0.0 3.570669 1819 4370 2995-2995 1.000000 52.0 atpH-atpI_p1 atpH-atpI 1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 20 20 45.0 45.0 58.0 58.5 1.4 0.0 0.556109 13237 14394 1219-1247 1.000000 77.7 atpH-atpI_p2 atpH-atpI 2 TACCCTCTACAGCTTGACCC TTTTTGCAACTTTAGCCGCG 20 20 55.0 45.0 58.1 58.5 0.0 0.0 0.655741 13237 14394 1302-1330 1.000000 76.8 atpH-atpI_p3 atpH-atpI 3 GCGATACCCTCTACAGCTTG TTTTTGCAACTTTAGCCGCG 20 20 55.0 45.0 57.9 58.5 0.0 0.0 0.659258 13237 14394 1306-1334 1.000000 76.8 atpH-atpI_p4 atpH-atpI 4 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 20 20 45.0 45.0 58.2 58.5 1.4 0.0 0.701648 13237 14394 1220-1248 1.000000 76.6 atpH-atpI_p5 atpH-atpI 5 ATAACGGAAGCGGCAGAAAT CCGCGGCTTATATAGGTGAA 20 20 45.0 50.0 58.0 57.3 1.4 14.7 0.774530 13237 14394 1204-1232 1.000000 76.0 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GCCCTTTTAACTCAGCGGTA GAAACGGTCCCTACGTAACC 20 20 50.0 55.0 57.9 58.0 0.0 5.0 0.107245 32372 33629 1414-1419 1.000000 79.7 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.216651 32372 33629 1432-1437 1.000000 78.8 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 20 20 50.0 55.0 58.3 58.0 0.0 5.0 0.286514 32372 33629 1377-1382 1.000000 78.3 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.9 58.2 0.0 0.0 0.349607 32372 33629 1422-1427 1.000000 77.8 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 32372 33629 1395-1400 1.000000 77.4 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.9 57.8 5.2 0.0 0.336606 51358 52554 885-1336 1.000000 82.0 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 20 20 55.0 50.0 58.3 57.8 0.0 0.0 0.528864 51358 52554 902-1353 1.000000 80.4 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.7 57.8 0.0 0.0 0.539129 51358 52554 897-1348 1.000000 80.4 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 20 20 50.0 55.0 57.9 57.5 5.2 0.0 0.680379 51358 52554 884-1335 1.000000 79.2 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.5 57.8 0.0 0.0 0.694593 51358 52554 898-1349 1.000000 79.1 trnM-CAU-atpE_p1 trnM-CAU-atpE 1 GGCGAGAGTCATTGGTTCAA CGGGTAGAGGCTATCAATGC 20 20 50.0 55.0 58.2 57.9 0.0 0.0 0.277644 53460 53704 299-307 1.000000 81.5 trnM-CAU-atpE_p2 trnM-CAU-atpE 2 GGCGAGAGTCATTGGTTCAA GGGTAGAGGCTATCAATGCG 20 20 50.0 55.0 58.2 57.9 0.0 0.0 0.277644 53460 53704 298-306 1.000000 81.5 trnM-CAU-atpE_p3 trnM-CAU-atpE 3 GGCGAGAGTCATTGGTTCAA GGTAGAGGCTATCAATGCGG 20 20 50.0 55.0 58.2 57.9 0.0 0.0 0.277644 53460 53704 297-305 1.000000 81.5 trnM-CAU-atpE_p4 trnM-CAU-atpE 4 GGCGAGAGTCATTGGTTCAA ACACGGGTAGAGGCTATCAA 20 20 50.0 50.0 58.2 57.8 0.0 0.0 0.346156 53460 53704 302-310 1.000000 80.9 trnM-CAU-atpE_p5 trnM-CAU-atpE 5 ATTGCTTTCATACGGCGAGA GGTAGAGGCTATCAATGCGG 20 20 45.0 55.0 57.7 57.9 16.6 0.0 0.395582 53460 53704 310-318 1.000000 80.4 ycf4-cemA_p1 ycf4-cemA 1 TTGGCCTATTTCTTGCGTGT GAGAGAGAAAGCCACCAAGG 20 20 45.0 55.0 58.1 57.9 3.3 0.0 0.202190 61706 62398 792-800 1.000000 79.8 ycf4-cemA_p2 ycf4-cemA 2 AAAAAGCTGCTGAATTGGCC GAGAGAGAAAGCCACCAAGG 20 20 45.0 55.0 57.8 57.9 1.4 0.0 0.280841 61706 62398 806-814 1.000000 79.1 ycf4-cemA_p3 ycf4-cemA 3 AAAGCTGCTGAATTGGCCTA GAGAGAGAAAGCCACCAAGG 20 20 45.0 55.0 57.8 57.9 1.4 0.0 0.327130 61706 62398 804-812 1.000000 78.8 ycf4-cemA_p4 ycf4-cemA 4 AAGCTGCTGAATTGGCCTAT GAGAGAGAAAGCCACCAAGG 20 20 45.0 55.0 57.6 57.9 1.4 0.0 0.555614 61706 62398 803-811 1.000000 76.9 ycf4-cemA_p5 ycf4-cemA 5 AGCTGCTGAATTGGCCTATT GAGAGAGAAAGCCACCAAGG 20 20 45.0 55.0 57.6 57.9 12.5 0.0 0.555614 61706 62398 802-810 1.000000 76.9 petA-psbJ_p1 petA-psbJ 1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.0 57.6 0.0 4.1 0.403359 64273 65259 1132-1147 1.000000 77.8 petA-psbJ_p2 petA-psbJ 2 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 57.8 57.6 0.0 4.1 0.519826 64273 65259 1115-1130 1.000000 76.9 petA-psbJ_p3 petA-psbJ 3 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.2 57.6 5.4 4.1 0.543756 64273 65259 1046-1061 1.000000 76.8 petA-psbJ_p4 petA-psbJ 4 TCGAGAAGGTTCAATTGGCC ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.2 57.6 5.4 4.1 0.543756 64273 65259 1048-1063 1.000000 76.8 petA-psbJ_p5 petA-psbJ 5 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.2 57.6 13.8 4.1 0.543756 64273 65259 1129-1144 1.000000 76.7 psbE-petL_p1 psbE-petL 1 AAAGGATCAAAACGGCCAGT AGCGCTCTTAGTTCAGTTCG 20 20 45.0 50.0 58.0 58.0 2.2 2.6 0.020651 66038 67127 1880-1881 1.000000 80.6 psbE-petL_p2 psbE-petL 2 AAGGATCAAAACGGCCAGTT AGCGCTCTTAGTTCAGTTCG 20 20 45.0 50.0 58.0 58.0 2.2 2.6 0.020651 66038 67127 1879-1880 1.000000 80.6 psbE-petL_p3 psbE-petL 3 AAAGGATCAAAACGGCCAGT AGAACGTGGGTCTCCAAAAC 20 20 45.0 50.0 58.0 58.0 2.2 0.0 0.041452 66038 67127 1858-1859 1.000000 80.5 psbE-petL_p4 psbE-petL 4 AAGGATCAAAACGGCCAGTT AGAACGTGGGTCTCCAAAAC 20 20 45.0 50.0 58.0 58.0 2.2 0.0 0.041452 66038 67127 1857-1858 1.000000 80.5 psbE-petL_p5 psbE-petL 5 TCGTTCGGGTTTGATTGTGT AGCGCTCTTAGTTCAGTTCG 20 20 45.0 50.0 58.0 58.0 0.0 2.6 0.059731 66038 67127 2089-2090 1.000000 80.2 trnP-UGG-psaJ_p1 trnP-UGG-psaJ 1 ACCCGTGACATTTTGTACCC GTACTTAGTACTGGCGCCAC 20 20 50.0 55.0 58.1 58.1 0.0 29.0 0.181160 67924 68274 453-453 1.000000 79.3 trnP-UGG-psaJ_p2 trnP-UGG-psaJ 2 CCCGTGACATTTTGTACCCA GTACTTAGTACTGGCGCCAC 20 20 50.0 55.0 58.1 58.1 0.0 29.0 0.182504 67924 68274 452-452 1.000000 79.2 trnP-UGG-psaJ_p3 trnP-UGG-psaJ 3 ACAGGATTTGAACCCGTGAC GTACTTAGTACTGGCGCCAC 20 20 50.0 55.0 58.1 58.1 0.0 29.0 0.189196 67924 68274 464-464 1.000000 79.2 trnP-UGG-psaJ_p4 trnP-UGG-psaJ 4 CAGGATTTGAACCCGTGACA GTACTTAGTACTGGCGCCAC 20 20 50.0 55.0 58.1 58.1 0.0 29.0 0.190529 67924 68274 463-463 1.000000 79.2 trnP-UGG-psaJ_p5 trnP-UGG-psaJ 5 TGACAGGATTTGAACCCGTG GTACTTAGTACTGGCGCCAC 20 20 50.0 55.0 58.1 58.1 0.0 29.0 0.190529 67924 68274 466-466 1.000000 79.2 clpP_p1 clpP 1 ACATGTTCCTCGACGTTGAG TTCGTTACAGTCCCTCCTGT 20 20 50.0 50.0 57.9 58.0 23.7 0.0 0.071363 71225 73255 2971-2974 1.000000 79.8 clpP_p2 clpP 2 TGCACCAACGGAAACCATAA ATACCAAGGCAAACCCATGG 20 20 45.0 50.0 58.0 58.1 0.0 4.1 0.152671 71225 73255 2923-2925 1.000000 79.2 clpP_p3 clpP 3 TTGCACCAACGGAAACCATA ATACCAAGGCAAACCCATGG 20 20 45.0 50.0 58.0 58.1 0.0 4.1 0.152671 71225 73255 2924-2926 1.000000 79.2 clpP_p4 clpP 4 ACATGTTCCTCGACGTTGAG ATACCAAGGCAAACCCATGG 20 20 50.0 50.0 57.9 58.1 23.7 4.1 0.201324 71225 73255 2729-2732 1.000000 78.8 clpP_p5 clpP 5 ACATGTTCCTCGACGTTGAG GTCCCTCCTGTGATACTCCA 20 20 50.0 55.0 57.9 57.8 23.7 0.0 0.226713 71225 73255 2962-2965 1.000000 78.6 rpl2-rpl23_p1 rpl2-rpl23 1 CCACAACGATGCTGTCCATA CGCTTCAACCGGGTTATTCT 20 20 50.0 50.0 58.0 58.3 0.0 13.0 0.288602 87001 87018 181-181 1.000000 78.1 rpl2-rpl23_p2 rpl2-rpl23 2 ACTGTCTACGGTTCCATTGC CGCTTCAACCGGGTTATTCT 20 20 50.0 50.0 57.9 58.3 0.0 13.0 0.360600 87001 87018 128-128 1.000000 77.7 rpl2-rpl23_p3 rpl2-rpl23 3 CCACAACGATGCTGTCCATA ATCATTACGCTTCAACCGGG 20 20 50.0 50.0 58.0 58.3 0.0 6.3 0.359070 87001 87018 188-188 1.000000 77.6 rpl2-rpl23_p4 rpl2-rpl23 4 CCACAACGATGCTGTCCATA TCCGGGAAAGGGTAGAAGAA 20 20 50.0 50.0 58.0 57.7 0.0 0.0 0.353426 87001 87018 249-249 1.000000 77.5 rpl2-rpl23_p5 rpl2-rpl23 5 CCACAACGATGCTGTCCATA TTCCGGGAAAGGGTAGAAGA 20 20 50.0 50.0 58.0 57.7 0.0 7.1 0.353426 87001 87018 250-250 1.000000 77.5 ndhF_p1 ndhF 1 ACAGGAACAAGAGGGATCCA TTCAAACTCGGGAAAAGGGG 20 20 50.0 50.0 58.0 58.0 15.2 0.0 0.055086 110929 113157 2767-2813 1.000000 81.5 ndhF_p2 ndhF 2 GCAGAATACCGTCACCCATT TTCAAACTCGGGAAAAGGGG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.048681 110929 113157 2891-2937 1.000000 81.4 ndhF_p3 ndhF 3 AAGAGGGATCCACCGAAGAA TTCAAACTCGGGAAAAGGGG 20 20 50.0 50.0 58.0 58.0 15.2 0.0 0.057672 110929 113157 2759-2805 1.000000 81.4 ndhF_p4 ndhF 4 AGGGTGGAAAGTGAGGAAGA TTCAAACTCGGGAAAAGGGG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.128697 110929 113157 2832-2878 1.000000 80.8 ndhF_p5 ndhF 5 AGGGATCCACCGAAGAAGAT TTCAAACTCGGGAAAAGGGG 20 20 50.0 50.0 57.8 58.0 15.2 0.0 0.192810 110929 113157 2756-2802 1.000000 80.4 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TGTGCAGCAAACAAATAAGT TTAGAAGATGACATGCCGCC 20 20 35.0 50.0 54.3 58.0 0.0 0.0 3.748004 114063 115033 1083-1090 1.000000 51.3 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TGTGCAGCAAACAAATAAGT TCCTAAGAGCAGCGTGTCTA 20 20 35.0 50.0 54.3 57.9 0.0 0.0 3.825840 114063 115033 1029-1036 1.000000 50.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TGTGCAGCAAACAAATAAGT AGCAGCGTGTCTACCAATTT 20 20 35.0 45.0 54.3 57.8 0.0 0.0 3.886567 114063 115033 1022-1029 1.000000 50.2 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TGTGCAGCAAACAAATAAGT CCTAAGAGCAGCGTGTCTAC 20 20 35.0 55.0 54.3 57.8 0.0 0.0 3.897858 114063 115033 1028-1035 1.000000 50.1 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TGTGCAGCAAACAAATAAGT CTAAGAGCAGCGTGTCTACC 20 20 35.0 55.0 54.3 57.8 0.0 0.0 3.897858 114063 115033 1027-1034 1.000000 50.1 ndhD_p1 ndhD 1 ACGAACCGTGCGAATCAATA CTGCCCGACCGATGTATTAG 20 20 45.0 55.0 58.0 57.9 5.7 0.0 0.066944 116427 117929 2004-2004 1.000000 80.2 ndhD_p2 ndhD 2 ACGAACCGTGCGAATCAATA TGTGAATCTGCCTGTCCAAC 20 20 45.0 50.0 58.0 58.1 5.7 0.0 0.104732 116427 117929 1904-1904 1.000000 79.9 ndhD_p3 ndhD 3 ACGAACCGTGCGAATCAATA TGATACCTTGGGACGGATGT 20 20 45.0 50.0 58.0 58.1 5.7 0.0 0.126822 116427 117929 1981-1981 1.000000 79.7 ndhD_p4 ndhD 4 ACGAACCGTGCGAATCAATA GGAAGACTGCGTTGGTTGTA 20 20 45.0 50.0 58.0 58.1 5.7 0.0 0.127254 116427 117929 1929-1929 1.000000 79.7 ndhD_p5 ndhD 5 ACGAACCGTGCGAATCAATA ACAAAACCCGTGCTCAAGAT 20 20 45.0 45.0 58.0 58.0 5.7 0.0 0.019695 116427 117929 1771-1771 0.666667 66.6 ndhD-psaC_p1 ndhD-psaC 1 AACCCGCAGATATTGGCAAA TGTGAATCTGCCTGTCCAAC 20 20 45.0 50.0 58.2 58.1 3.0 0.0 0.257774 117930 118044 257-257 1.000000 82.0 ndhD-psaC_p2 ndhD-psaC 2 ACCCGCAGATATTGGCAAAA TGTGAATCTGCCTGTCCAAC 20 20 45.0 50.0 58.2 58.1 3.0 0.0 0.257774 117930 118044 256-256 1.000000 82.0 ndhD-psaC_p3 ndhD-psaC 3 CCCGCAGATATTGGCAAAAC TGTGAATCTGCCTGTCCAAC 20 20 50.0 50.0 57.8 58.1 3.0 0.0 0.312106 117930 118044 255-255 1.000000 81.6 ndhD-psaC_p4 ndhD-psaC 4 ACCCGCAGATATTGGCAAAA GGAAGACTGCGTTGGTTGTA 20 20 45.0 50.0 58.2 58.1 3.0 0.0 0.280296 117930 118044 281-281 1.000000 81.5 ndhD-psaC_p5 ndhD-psaC 5 AACCCGCAGATATTGGCAAA GGAAGACTGCGTTGGTTGTA 20 20 45.0 50.0 58.2 58.1 3.0 0.0 0.280296 117930 118044 282-282 1.000000 81.4 ycf1_p1 ycf1 1 TCGAATTGAGAGATTAATCGGACA TCTGACTGATAGAACAAGCACA 24 22 37.5 40.9 57.9 57.1 9.8 0.0 7.016011 126632 126751 189-189 1.000000 42.1 ycf1_p2 ycf1 2 TCGAATTGAGAGATTAATCGGACA GACGGATCTGACTGATAGAACA 24 22 37.5 45.5 57.9 57.1 9.8 9.0 7.046520 126632 126751 195-195 1.000000 42.1 ycf1_p3 ycf1 3 TCGAATTGAGAGATTAATCGGACA GGATCTGACTGATAGAACAAGCA 24 23 37.5 43.5 57.9 58.0 9.8 0.0 7.160128 126632 126751 192-192 1.000000 42.1 ycf1_p4 ycf1 4 TCGAATTGAGAGATTAATCGGACA ACGGATCTGACTGATAGAACA 24 21 37.5 42.9 57.9 55.9 9.8 9.0 7.229088 126632 126751 194-194 1.000000 42.1 ycf1_p5 ycf1 5 TCGAATTGAGAGATTAATCGGACA AGACGGATCTGACTGATAGAAC 24 22 37.5 45.5 57.9 56.8 9.8 9.0 7.318224 126632 126751 196-196 1.000000 42.0 rpl23-rpl2_p1 rpl23-rpl2 1 CGCTTCAACCGGGTTATTCT CCACAACGATGCTGTCCATA 20 20 50.0 50.0 58.3 58.0 13.0 0.0 0.288602 152672 152689 181-181 1.000000 78.1 rpl23-rpl2_p2 rpl23-rpl2 2 CGCTTCAACCGGGTTATTCT ACTGTCTACGGTTCCATTGC 20 20 50.0 50.0 58.3 57.9 13.0 0.0 0.360600 152672 152689 128-128 1.000000 77.7 rpl23-rpl2_p3 rpl23-rpl2 3 ATCATTACGCTTCAACCGGG CCACAACGATGCTGTCCATA 20 20 50.0 50.0 58.3 58.0 6.3 0.0 0.359070 152672 152689 188-188 1.000000 77.6 rpl23-rpl2_p4 rpl23-rpl2 4 TCCGGGAAAGGGTAGAAGAA CCACAACGATGCTGTCCATA 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.353426 152672 152689 249-249 1.000000 77.5 rpl23-rpl2_p5 rpl23-rpl2 5 TTCCGGGAAAGGGTAGAAGA CCACAACGATGCTGTCCATA 20 20 50.0 50.0 57.7 58.0 7.1 0.0 0.353426 152672 152689 250-250 1.000000 77.5