primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
DXZ06_pgt001-psbA_p1	DXZ06_pgt001-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	75	490	535-535	1.000000	77.2
DXZ06_pgt001-psbA_p2	DXZ06_pgt001-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	75	490	549-549	1.000000	77.1
DXZ06_pgt001-psbA_p3	DXZ06_pgt001-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	490	548-548	1.000000	77.1
DXZ06_pgt001-psbA_p4	DXZ06_pgt001-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	490	550-550	1.000000	77.1
DXZ06_pgt001-psbA_p5	DXZ06_pgt001-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	75	490	536-536	1.000000	76.7
psbA-matK_p1	psbA-matK	1	TAAAGACGGTTTTCGGTGCT	CGCGAAGGTTATCTAGAAGGC	20	21	45.0	52.4	57.8	58.6	0.0	0.0	1.847948	1553	2088	695-696	1.000000	65.3
psbA-matK_p2	psbA-matK	2	TAAAGACGGTTTTCGGTGCT	GCGAAGGTTATCTAGAAGGCG	20	21	45.0	52.4	57.8	58.6	0.0	0.0	1.847948	1553	2088	694-695	1.000000	65.3
psbA-matK_p3	psbA-matK	3	GTAAAGACGGTTTTCGGTGC	CGCGAAGGTTATCTAGAAGGC	20	21	50.0	52.4	57.7	58.6	0.0	0.0	1.909598	1553	2088	696-697	1.000000	64.8
psbA-matK_p4	psbA-matK	4	GTAAAGACGGTTTTCGGTGC	GCGAAGGTTATCTAGAAGGCG	20	21	50.0	52.4	57.7	58.6	0.0	0.0	1.909598	1553	2088	695-696	1.000000	64.8
psbA-matK_p5	psbA-matK	5	TTTTCGGTGCTGGTTATCCA	GCGAAGGTTATCTAGAAGGCG	20	21	45.0	52.4	57.4	58.6	0.0	0.0	2.174761	1553	2088	685-686	1.000000	62.7
matK_p1	matK	1	ATTTACAACGTTTCATAAGGAA	CCTTGTTTTGACTGTATCGCA	22	21	27.3	42.9	52.1	56.8	0.0	0.0	10.114120	2089	3609	1621-1621	1.000000	40.0
matK_p2	matK	2	ATTTACAACGTTTCATAAGGAA	ACCTTGTTTTGACTGTATCGC	22	21	27.3	42.9	52.1	56.8	0.0	0.0	10.116154	2089	3609	1622-1622	1.000000	40.0
matK_p3	matK	3	ATTTACAACGTTTCATAAGGAA	CCTTGTTTTGACTGTATCGCAC	22	22	27.3	45.5	52.1	58.2	0.0	0.0	10.136259	2089	3609	1621-1621	1.000000	40.0
matK_p4	matK	4	ATTTACAACGTTTCATAAGGAA	CCGAGGTATCTATTCGGTTTGT	22	22	27.3	45.5	52.1	57.7	0.0	4.1	10.264303	2089	3609	1652-1652	1.000000	40.0
matK_p5	matK	5	ATTTACAACGTTTCATAAGGAA	ACCTTGTTTTGACTGTATCGCA	22	22	27.3	40.9	52.1	58.5	0.0	0.0	10.473981	2089	3609	1622-1622	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3610	4347	870-870	1.000000	58.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3610	4347	869-869	1.000000	58.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3610	4347	885-885	1.000000	57.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3610	4347	883-883	1.000000	56.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3610	4347	870-870	1.000000	54.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4385	5211	1008-1009	1.000000	76.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4385	5211	1007-1008	1.000000	76.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4385	5211	947-948	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4385	5211	946-947	1.000000	76.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4385	5211	1032-1033	1.000000	75.7
trnC-GCA-psbM_p1	trnC-GCA-psbM	1	GTTGTGTATCGTTTTGGCGG	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.0	55.6	0.0	0.0	2.464176	9273	10722	1624-1625	1.000000	60.3
trnC-GCA-psbM_p2	trnC-GCA-psbM	2	TTTCCCCAGTTCAAATCCGG	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.1	55.6	0.0	0.0	2.504721	9273	10722	1567-1568	1.000000	60.0
trnC-GCA-psbM_p3	trnC-GCA-psbM	3	GTTGTGTATCGTTTTGGCGG	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.0	56.4	0.0	0.0	2.675987	9273	10722	1624-1625	1.000000	58.6
trnC-GCA-psbM_p4	trnC-GCA-psbM	4	TTTCCCCAGTTCAAATCCGG	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.1	56.4	0.0	0.0	2.716532	9273	10722	1567-1568	1.000000	58.3
trnC-GCA-psbM_p5	trnC-GCA-psbM	5	GGGGGACTGCAAATCCTTTT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.4	55.6	14.7	0.0	2.778084	9273	10722	1585-1586	1.000000	57.8
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGTAGAAAGGCAGTAGGAACT	TTCAATAGGCAAGAGCACCG	21	20	42.9	50.0	55.7	58.3	0.0	0.0	3.550437	10828	11486	790-791	1.000000	51.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10828	11486	775-776	1.000000	50.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10828	11486	769-770	1.000000	50.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAGTAGAAAGGCAGTAGGAACT	TTCAATAGGCAAGAGCACCG	22	20	40.9	50.0	56.5	58.3	0.0	0.0	3.786412	10828	11486	791-792	1.000000	49.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	10828	11486	772-773	1.000000	49.1
rpoC1_p1	rpoC1	1	TCTGAGAAGACTTTCCAGCT	GTACCTCCATATTGCGCTGA	20	20	45.0	50.0	55.8	57.5	11.0	9.7	2.730380	16120	18943	2978-2979	1.000000	58.2
rpoC1_p2	rpoC1	2	TCTGAGAAGACTTTCCAGCT	TCAGTACCTCCATATTGCGC	20	20	45.0	50.0	55.8	57.5	11.0	0.0	2.730380	16120	18943	2981-2982	1.000000	58.2
rpoC1_p3	rpoC1	3	TCTGAGAAGACTTTCCAGCT	AGTACCTCCATATTGCGCTG	20	20	45.0	50.0	55.8	57.5	11.0	9.7	2.737343	16120	18943	2979-2980	1.000000	58.1
rpoC1_p4	rpoC1	4	TCTGAGAAGACTTTCCAGCT	CAGTACCTCCATATTGCGCT	20	20	45.0	50.0	55.8	57.5	11.0	9.7	2.737343	16120	18943	2980-2981	1.000000	58.1
rpoC1_p5	rpoC1	5	TCTGAGAAGACTTTCCAGCTT	GTACCTCCATATTGCGCTGA	21	20	42.9	50.0	56.6	57.5	11.0	9.7	2.940611	16120	18943	2978-2979	1.000000	56.5
rpoC2_p1	rpoC2	1	TGGAGGTACTGATGGCAGAA	CAGCGTCTTGAACTAGCCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.064526	19138	19257	237-237	1.000000	79.8
rpoC2_p2	rpoC2	2	GAGGTACTGATGGCAGAACG	CAGCGTCTTGAACTAGCCAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087841	19138	19257	235-235	1.000000	79.6
rpoC2_p3	rpoC2	3	TGGAGGTACTGATGGCAGAA	TTCAGCGTCTTGAACTAGCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129077	19138	19257	239-239	1.000000	79.3
rpoC2_p4	rpoC2	4	GAGGTACTGATGGCAGAACG	TTCAGCGTCTTGAACTAGCC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.152391	19138	19257	237-237	1.000000	79.1
rpoC2_p5	rpoC2	5	ATGGAGGTACTGATGGCAGA	CAGCGTCTTGAACTAGCCAT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.196681	19138	19257	238-238	1.000000	78.8
trnR-UCU-DXZ06_pgt010_p1	trnR-UCU-DXZ06_pgt010	1	AGAAGACCTCTGTCCTATCCA	ATTTGAACCGAGGGCTTACG	21	20	47.6	50.0	56.9	58.3	0.0	0.0	2.317442	29939	31147	1301-1301	1.000000	61.5
trnR-UCU-DXZ06_pgt010_p2	trnR-UCU-DXZ06_pgt010	2	AGGTTTAGAAGACCTCTGTCCT	ATTTGAACCGAGGGCTTACG	22	20	45.5	50.0	57.9	58.3	0.0	0.0	2.400770	29939	31147	1307-1307	1.000000	60.9
trnR-UCU-DXZ06_pgt010_p3	trnR-UCU-DXZ06_pgt010	3	AGAAGACCTCTGTCCTATCCA	GGATTTGAACCGAGGGCTTA	21	20	47.6	50.0	56.9	57.6	0.0	0.0	2.464914	29939	31147	1303-1303	1.000000	60.3
trnR-UCU-DXZ06_pgt010_p4	trnR-UCU-DXZ06_pgt010	4	AGGTTTAGAAGACCTCTGTCCT	GGATTTGAACCGAGGGCTTA	22	20	45.5	50.0	57.9	57.6	0.0	0.0	2.548242	29939	31147	1309-1309	1.000000	59.7
trnR-UCU-DXZ06_pgt010_p5	trnR-UCU-DXZ06_pgt010	5	AGAAGACCTCTGTCCTATCCA	ACGCCTTACCATGGGATTAC	21	20	47.6	50.0	56.9	57.4	0.0	18.1	2.684591	29939	31147	1284-1284	1.000000	58.6
ycf3-trnS-GCU_p1	ycf3-trnS-GCU	1	TTCTCCTGAAGTTGTCGGAA	AAGCAAACGCCTACATAGCA	20	20	45.0	45.0	56.4	57.9	0.0	0.0	1.692121	44066	44977	1067-1067	1.000000	66.5
ycf3-trnS-GCU_p2	ycf3-trnS-GCU	2	TTTCTCCTGAAGTTGTCGGA	AAGCAAACGCCTACATAGCA	20	20	45.0	45.0	56.4	57.9	0.0	0.0	1.692121	44066	44977	1068-1068	1.000000	66.5
ycf3-trnS-GCU_p3	ycf3-trnS-GCU	3	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44066	44977	1041-1041	1.000000	65.9
ycf3-trnS-GCU_p4	ycf3-trnS-GCU	4	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.769175	44066	44977	1042-1042	1.000000	65.9
ycf3-trnS-GCU_p5	ycf3-trnS-GCU	5	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	20	20	45.0	55.0	56.4	57.8	0.0	0.0	1.804650	44066	44977	1084-1084	1.000000	65.6
rps4-trnL-UAA_p1	rps4-trnL-UAA	1	TTCCAGCTTTGGGCCTTTTA	GCGTCTACCTATTTCGCCAT	20	20	45.0	50.0	57.6	57.8	4.5	0.0	0.540622	45997	46936	1053-1054	1.000000	75.8
rps4-trnL-UAA_p2	rps4-trnL-UAA	2	AGATCACTTCCAGCTTTGGG	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.654292	45997	46936	1060-1061	1.000000	74.8
rps4-trnL-UAA_p3	rps4-trnL-UAA	3	TCCAGCTTTGGGCCTTTTAT	GCGTCTACCTATTTCGCCAT	20	20	45.0	50.0	57.4	57.8	4.5	0.0	0.771953	45997	46936	1052-1053	1.000000	73.9
rps4-trnL-UAA_p4	rps4-trnL-UAA	4	TTCCAGCTTTGGGCCTTTTA	AAGTCCGTAGCGTCTACCTA	20	20	45.0	50.0	57.6	57.3	4.5	10.2	1.089080	45997	46936	1062-1063	1.000000	71.4
rps4-trnL-UAA_p5	rps4-trnL-UAA	5	TTCCAGCTTTGGGCCTTTTA	TAAGTCCGTAGCGTCTACCT	20	20	45.0	50.0	57.6	57.3	4.5	10.2	1.089080	45997	46936	1063-1064	1.000000	71.4
psbH-petB_p1	psbH-petB	1	GTCGCAATGGCTCTATTTGC	ATATCATCCGCAATCGCCTG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.380540	74713	75603	1071-1076	1.000000	77.4
psbH-petB_p2	psbH-petB	2	GTCGCAATGGCTCTATTTGC	TCTCGAGACGCTCTTCAAAC	20	20	50.0	50.0	57.9	57.7	0.0	12.1	0.444430	74713	75603	1049-1054	1.000000	76.9
psbH-petB_p3	psbH-petB	3	GTCGCAATGGCTCTATTTGC	CCCCCTCCGAGAACTGTATA	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.487736	74713	75603	997-1002	1.000000	76.6
psbH-petB_p4	psbH-petB	4	CTTTGATGGGTGTCGCAATG	ATATCATCCGCAATCGCCTG	20	20	50.0	50.0	57.7	58.3	0.0	0.0	0.536687	74713	75603	1082-1087	1.000000	76.1
psbH-petB_p5	psbH-petB	5	CTTTGATGGGTGTCGCAATG	TCTCGAGACGCTCTTCAAAC	20	20	50.0	50.0	57.7	57.7	0.0	12.1	0.600577	74713	75603	1060-1065	1.000000	75.6
rps8-rpl14_p1	rps8-rpl14	1	CTCGAACCACGCCTTTTCTA	TGCGATTGCCAGAGAATTGA	20	20	50.0	45.0	57.9	57.9	0.0	1.5	0.186927	80449	80646	343-344	1.000000	78.7
rps8-rpl14_p2	rps8-rpl14	2	CTCGAACCACGCCTTTTCTA	GTGCGATTGCCAGAGAATTG	20	20	50.0	50.0	57.9	57.8	0.0	1.5	0.258932	80449	80646	344-345	1.000000	78.2
rps8-rpl14_p3	rps8-rpl14	3	CTACTCGAACCACGCCTTTT	TGCGATTGCCAGAGAATTGA	20	20	50.0	45.0	58.2	57.9	0.0	1.5	0.320374	80449	80646	346-347	1.000000	77.7
rps8-rpl14_p4	rps8-rpl14	4	TACTCGAACCACGCCTTTTC	TGCGATTGCCAGAGAATTGA	20	20	50.0	45.0	58.2	57.9	0.0	1.5	0.325367	80449	80646	345-346	1.000000	77.6
rps8-rpl14_p5	rps8-rpl14	5	CTACTCGAACCACGCCTTTT	GTGCGATTGCCAGAGAATTG	20	20	50.0	50.0	58.2	57.8	0.0	1.5	0.392379	80449	80646	347-348	1.000000	77.1
ycf2_p1	ycf2	1	AGCAGAAGAGCCGTTTTCAA	AACTGTGGATCATTCGGACC	20	20	45.0	50.0	58.0	57.6	0.0	0.0	0.435933	88972	89091	238-238	1.000000	76.8
ycf2_p2	ycf2	2	AGCAGAAGAGCCGTTTTCAA	ACTGTGGATCATTCGGACCT	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.459574	88972	89091	237-237	1.000000	76.7
ycf2_p3	ycf2	3	ATGAGCAGAAGAGCCGTTTT	AACTGTGGATCATTCGGACC	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.594379	88972	89091	241-241	1.000000	75.6
ycf2_p4	ycf2	4	ATGAGCAGAAGAGCCGTTTT	ACTGTGGATCATTCGGACCT	20	20	45.0	50.0	57.8	58.4	0.0	0.0	0.618020	88972	89091	240-240	1.000000	75.4
ycf2_p5	ycf2	5	GCAGAAGAGCCGTTTTCAAG	AACTGTGGATCATTCGGACC	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.725623	88972	89091	237-237	1.000000	74.5
ccsA_p1	ccsA	1	GCCGGTTGAGATAAGGTGTT	GCGGCATGCCATCTTCTAAA	20	20	50.0	50.0	57.9	59.0	1.9	34.9	1.085153	122117	123085	1157-1157	1.000000	71.4
ccsA_p2	ccsA	2	TGATTCACATGGTTCTCGCC	GCGGCATGCCATCTTCTAAA	20	20	50.0	50.0	58.3	59.0	0.0	34.9	1.237890	122117	123085	1174-1174	1.000000	70.2
ccsA_p3	ccsA	3	GTTCTCGCCGGTTGAGATAA	GCGGCATGCCATCTTCTAAA	20	20	50.0	50.0	57.7	59.0	23.5	34.9	1.264689	122117	123085	1163-1163	1.000000	70.0
ccsA_p4	ccsA	4	ACCCCGTGAATCACTACACT	GCGGCATGCCATCTTCTAAA	20	20	50.0	50.0	58.4	59.0	11.9	34.9	1.334812	122117	123085	1197-1197	1.000000	69.4
ccsA_p5	ccsA	5	GCCGGTTGAGATAAGGTGTT	GCGGCATGCCATCTTCTAA	20	19	50.0	52.6	57.9	58.3	1.9	34.9	1.402789	122117	123085	1157-1157	1.000000	68.8