primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcR_p1	rbcR	1	GGTAGACGCGCTAGGTTTAG	CGTACTACCCGCACCTTTTT	20	20	55.0	50.0	57.9	58.2	5.7	0.0	0.317244	491	1414	1487-1487	0.500000	57.5
rbcR_p2	rbcR	2	GTAGACGCGCTAGGTTTAGG	CGTACTACCCGCACCTTTTT	20	20	55.0	50.0	57.9	58.2	5.7	0.0	0.317244	491	1414	1486-1486	0.500000	57.5
rbcR_p3	rbcR	3	GGTAGACGCGCTAGGTTTAG	TTTTTCGTACTACCCGCACC	20	20	55.0	50.0	57.9	58.2	5.7	0.0	0.322275	491	1414	1492-1492	0.500000	57.4
rbcR_p4	rbcR	4	GTAGACGCGCTAGGTTTAGG	TTTTTCGTACTACCCGCACC	20	20	55.0	50.0	57.9	58.2	5.7	0.0	0.322275	491	1414	1491-1491	0.500000	57.4
rbcR_p5	rbcR	5	TGAAAGATAACGGGTGTGGC	CGTACTACCCGCACCTTTTT	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.384382	491	1414	1513-1513	0.500000	56.9
psbA-ycf35_p1	psbA-ycf35	1	GGGGAATTTCAGGTTCCTCC	GCTTCAGGGTCTTCCTTGTC	20	20	55.0	55.0	57.9	58.2	5.0	0.0	0.330604	9275	9394	562-562	0.500000	57.4
psbA-ycf35_p2	psbA-ycf35	2	GGGGAATTTCAGGTTCCTCC	TCAGGGTCTTCCTTGTCCAA	20	20	55.0	50.0	57.9	58.2	5.0	0.0	0.330968	9275	9394	559-559	0.500000	57.4
psbA-ycf35_p3	psbA-ycf35	3	GGGGAATTTCAGGTTCCTCC	TTCAGGGTCTTCCTTGTCCA	20	20	55.0	50.0	57.9	58.2	5.0	0.0	0.330968	9275	9394	560-560	0.500000	57.4
psbA-ycf35_p4	psbA-ycf35	4	GGGGAATTTCAGGTTCCTCC	TGAGCAACTTCCAAGCGAAA	20	20	55.0	45.0	57.9	58.3	5.0	0.0	0.471037	9275	9394	287-287	0.500000	56.2
psbA-ycf35_p5	psbA-ycf35	5	GGGGAATTTCAGGTTCCTCC	TTGAGCAACTTCCAAGCGAA	20	20	55.0	45.0	57.9	58.3	5.0	0.0	0.471037	9275	9394	288-288	0.500000	56.2
ycf35-groEL_p1	ycf35-groEL	1	AAAAGTCTCCGTCATGGCAG	AAGCTCGACGAGTTTTGGTT	20	20	50.0	45.0	58.2	58.1	0.0	24.8	0.237021	12525	13358	2034-2034	0.500000	58.1
ycf35-groEL_p2	ycf35-groEL	2	CCGTCATGGCAGAGACTTTT	AAGCTCGACGAGTTTTGGTT	20	20	50.0	45.0	58.2	58.1	17.3	24.8	0.237021	12525	13358	2026-2026	0.500000	58.1
ycf35-groEL_p3	ycf35-groEL	3	AAAAGTCTCCGTCATGGCAG	TGCGGGAATTAATGGTCCAG	20	20	50.0	50.0	58.2	57.9	0.0	8.2	0.237821	12525	13358	1613-1613	0.500000	58.1
ycf35-groEL_p4	ycf35-groEL	4	CCGTCATGGCAGAGACTTTT	TGCGGGAATTAATGGTCCAG	20	20	50.0	50.0	58.2	57.9	17.3	8.2	0.237821	12525	13358	1605-1605	0.500000	58.1
ycf35-groEL_p5	ycf35-groEL	5	CCGTCATGGCAGAGACTTTT	GCGGGAATTAATGGTCCAGT	20	20	50.0	50.0	58.2	57.9	17.3	8.2	0.239269	12525	13358	1604-1604	0.500000	58.1
groEL_p1	groEL	1	ACTTGTTTGACCTTAGCCACT	TGGCGACACCCCAAAATTTA	21	20	42.9	45.0	57.4	58.0	0.0	0.0	1.581007	13359	14948	2153-2153	0.500000	47.4
groEL_p2	groEL	2	ACTTTGGTACGGAATAAGCGA	TGGCGACACCCCAAAATTTA	21	20	42.9	45.0	57.4	58.0	0.0	0.0	1.601220	13359	14948	2074-2074	0.500000	47.2
groEL_p3	groEL	3	TGGTACGGAATAAGCGAAGA	TGGCGACACCCCAAAATTTA	20	20	45.0	45.0	56.4	58.0	0.0	0.0	1.624225	13359	14948	2070-2070	0.500000	47.0
groEL_p4	groEL	4	ACTTGTTTGACCTTAGCCACT	ACCTACGAATGGTGGAGTCA	21	20	42.9	50.0	57.4	58.1	0.0	0.0	1.644784	13359	14948	2193-2193	0.500000	46.8
groEL_p5	groEL	5	ACTTGTTTGACCTTAGCCACT	CGAACCTACGAATGGTGGAG	21	20	42.9	55.0	57.4	58.1	0.0	0.0	1.655235	13359	14948	2196-2196	0.500000	46.8
tRNA-Gln-dnaK_p1	tRNA-Gln-dnaK	1	GCAGCTTGACGAATCAGAGA	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034492	15314	15877	1876-1876	0.500000	59.7
tRNA-Gln-dnaK_p2	tRNA-Gln-dnaK	2	TAAATTTTGGGGTGTCGCCA	GCAGGTATTTTAGCGGGTGA	20	20	45.0	50.0	58.0	58.0	4.5	0.0	0.035882	15314	15877	1360-1360	0.500000	59.7
tRNA-Gln-dnaK_p3	tRNA-Gln-dnaK	3	AGCAGCTTGACGAATCAGAG	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040041	15314	15877	1877-1877	0.500000	59.7
tRNA-Gln-dnaK_p4	tRNA-Gln-dnaK	4	GCAGCTTGACGAATCAGAGA	AGCAGGTATTTTAGCGGGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040199	15314	15877	1877-1877	0.500000	59.7
tRNA-Gln-dnaK_p5	tRNA-Gln-dnaK	5	TAAATTTTGGGGTGTCGCCA	AGCAGGTATTTTAGCGGGTG	20	20	45.0	50.0	58.0	58.0	4.5	0.0	0.041588	15314	15877	1361-1361	0.500000	59.7
ycf88_p1	ycf88	1	CGACCATTAACTCCATGGGG	AGCACGTGGTCGAATTCTTT	20	20	55.0	45.0	58.0	58.1	11.1	15.8	0.153728	20749	21270	1400-1419	1.000000	85.8
ycf88_p2	ycf88	2	AGGTCCGTTTGTTGCCTATC	AGCACGTGGTCGAATTCTTT	20	20	50.0	45.0	57.9	58.1	0.0	15.8	0.230472	20749	21270	1248-1267	1.000000	85.6
ycf88_p3	ycf88	3	AATGAACCCTTGTGACCACC	AGCACGTGGTCGAATTCTTT	20	20	50.0	45.0	58.0	58.1	0.0	15.8	0.125671	20749	21270	1480-1480	0.500000	59.0
ycf88_p4	ycf88	4	TCCAACAGTTCGTGGTTCAG	AGCACGTGGTCGAATTCTTT	20	20	50.0	45.0	58.1	58.1	0.0	15.8	0.172943	20749	21270	1501-1501	0.500000	58.6
ycf88_p5	ycf88	5	CCCAAATCGAGGTGGTCAAA	AGCACGTGGTCGAATTCTTT	20	20	50.0	45.0	58.1	58.1	14.3	15.8	0.216760	20749	21270	1711-1711	0.500000	58.3
secY_p1	secY	1	CGTGCATCAATTTGTGCCTT	TGAAACCTGAACTTCCTGCC	20	20	45.0	50.0	57.9	58.0	16.2	0.0	0.087580	26870	28117	2073-2073	0.500000	59.3
secY_p2	secY	2	GGTGGAGCTGTTCGTATTGT	TGAAACCTGAACTTCCTGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.113506	26870	28117	2163-2163	0.500000	59.1
secY_p3	secY	3	AGGTTCAGGTGTGATTGCAG	TGAAACCTGAACTTCCTGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117153	26870	28117	2182-2182	0.500000	59.1
secY_p4	secY	4	CGTGCATCAATTTGTGCCTT	ACGAGCTCCTTTGAAACCTG	20	20	45.0	50.0	57.9	58.1	16.2	8.5	0.184602	26870	28117	2084-2084	0.500000	58.5
secY_p5	secY	5	CGTGCATCAATTTGTGCCTT	TTGACCACGAACAGGTAAGC	20	20	45.0	50.0	57.9	58.1	16.2	0.0	0.195139	26870	28117	1826-1826	0.500000	58.4
rps16-clpC_p1	rps16-clpC	1	AGACGAAAAGGTGTTACCGG	CCGTTTCAGATGCTTCAGGA	20	20	50.0	50.0	57.8	57.9	0.0	2.1	0.258469	34917	35530	1366-1366	0.500000	57.9
rps16-clpC_p2	rps16-clpC	2	ATTGCTCAAGCTCGACAACT	CCGTTTCAGATGCTTCAGGA	20	20	45.0	50.0	57.8	57.9	3.3	2.1	0.275019	34917	35530	1276-1276	0.500000	57.8
rps16-clpC_p3	rps16-clpC	3	AGACGAAAAGGTGTTACCGG	TACACTTGCTGAGCAGTGTC	20	20	50.0	50.0	57.8	57.8	0.0	31.0	0.314181	34917	35530	1608-1608	0.500000	57.5
rps16-clpC_p4	rps16-clpC	4	ATTGCTCAAGCTCGACAACT	TACACTTGCTGAGCAGTGTC	20	20	45.0	50.0	57.8	57.8	3.3	31.0	0.330731	34917	35530	1518-1518	0.500000	57.4
rps16-clpC_p5	rps16-clpC	5	AGACGAAAAGGTGTTACCGG	GCAAAATCCGACCAAGGAGA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.338111	34917	35530	1985-1985	0.500000	57.3
rps6-tRNA-His_p1	rps6-tRNA-His	1	ACCTGAGGACAGAGTAGTGG	GTAATCTTAAGTGGGGGCCG	20	20	55.0	55.0	57.8	58.0	5.0	1.6	0.260631	44592	44711	833-833	0.500000	57.9
rps6-tRNA-His_p2	rps6-tRNA-His	2	ACCTGAGGACAGAGTAGTGG	TAATCTTAAGTGGGGGCCGA	20	20	55.0	50.0	57.8	58.1	5.0	1.6	0.341495	44592	44711	832-832	0.500000	57.3
rps6-tRNA-His_p3	rps6-tRNA-His	3	CCTTACCGATGGCAGAAGTT	GTAATCTTAAGTGGGGGCCG	20	20	50.0	55.0	57.6	58.0	0.0	1.6	0.440080	44592	44711	880-880	0.500000	56.5
rps6-tRNA-His_p4	rps6-tRNA-His	4	TCCTTACCGATGGCAGAAGT	GTAATCTTAAGTGGGGGCCG	20	20	50.0	55.0	58.4	58.0	0.0	1.6	0.468324	44592	44711	881-881	0.500000	56.3
rps6-tRNA-His_p5	rps6-tRNA-His	5	CCTTACCGATGGCAGAAGTT	TAATCTTAAGTGGGGGCCGA	20	20	50.0	50.0	57.6	58.1	0.0	1.6	0.520943	44592	44711	879-879	0.500000	55.8
5S ribosomal RNA-23S ribosomal RNA_p1	5S ribosomal RNA-23S ribosomal RNA	1	TCGAGATGGATCAGTGTGGT	AAGCATCTAAGTGGGAAGCC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.574210	62366	62485	284-300	1.000000	81.1
5S ribosomal RNA-23S ribosomal RNA_p2	5S ribosomal RNA-23S ribosomal RNA	2	CTATCTTCCCAGGAGGTCCC	AAGCATCTAAGTGGGAAGCC	20	20	60.0	50.0	58.3	57.6	0.0	0.0	0.703554	62366	62485	347-363	1.000000	79.7
5S ribosomal RNA-23S ribosomal RNA_p3	5S ribosomal RNA-23S ribosomal RNA	3	GATGGATCAGTGTGGTTCCG	AAGCATCTAAGTGGGAAGCC	20	20	55.0	50.0	58.3	57.6	0.0	0.0	0.770190	62366	62485	280-296	1.000000	79.5
5S ribosomal RNA-23S ribosomal RNA_p4	5S ribosomal RNA-23S ribosomal RNA	4	GATCAGTGTGGTTCCGCTTA	AAGCATCTAAGTGGGAAGCC	20	20	50.0	50.0	57.6	57.6	4.0	0.0	0.806926	62366	62485	276-292	1.000000	79.2
5S ribosomal RNA-23S ribosomal RNA_p5	5S ribosomal RNA-23S ribosomal RNA	5	ATCAGTGTGGTTCCGCTTAG	AAGCATCTAAGTGGGAAGCC	20	20	50.0	50.0	57.6	57.6	4.0	0.0	0.813503	62366	62485	275-291	1.000000	79.2
tRNA-Ala-tRNA-Ile_p1	tRNA-Ala-tRNA-Ile	1	ATGTGGAGGTAAGCGGAGT	CCCTGATAAGGGTGAGGTCT	19	20	52.6	55.0	58.0	57.8	0.0	7.1	1.190040	65485	65486	122-122	1.000000	71.1
tRNA-Ala-tRNA-Ile_p2	tRNA-Ala-tRNA-Ile	2	ATGTGGAGGTAAGCGGAGTC	CCCTGATAAGGGTGAGGTCT	20	20	55.0	55.0	59.2	57.8	0.0	7.1	1.357757	65485	65486	122-122	1.000000	69.8
tRNA-Ala-tRNA-Ile_p3	tRNA-Ala-tRNA-Ile	3	ACCAACTGAGCTATACCCCC	CTCAGTTGGTTAGAGCGCAC	20	20	55.0	55.0	58.5	58.9	0.0	11.5	1.420139	65485	65486	86-86	1.000000	69.6
tRNA-Ala-tRNA-Ile_p4	tRNA-Ala-tRNA-Ile	4	ACCAACTGAGCTATACCCCC	GGGCTATTAGCTCAGTTGGT	20	20	55.0	50.0	58.5	57.0	0.0	15.0	1.502515	65485	65486	96-96	1.000000	68.8
tRNA-Ala-tRNA-Ile_p5	tRNA-Ala-tRNA-Ile	5	GAGGTAAGCGGAGTCGAAC	CCCTGATAAGGGTGAGGTCT	19	20	57.9	55.0	57.7	57.8	0.0	7.1	1.508332	65485	65486	117-117	1.000000	68.6
tRNA-Pro-atpA_p1	tRNA-Pro-atpA	1	AAAGCAGTTCGAACCGGAAT	ACAAAAACAGTTAGCGCGTG	20	20	45.0	45.0	58.1	57.9	24.2	8.4	0.207424	68735	69202	1059-1059	0.500000	58.3
tRNA-Pro-atpA_p2	tRNA-Pro-atpA	2	AAAGCAGTTCGAACCGGAAT	ACACAAAAACAGTTAGCGCG	20	20	45.0	45.0	58.1	57.9	24.2	0.0	0.207424	68735	69202	1061-1061	0.500000	58.3
tRNA-Pro-atpA_p3	tRNA-Pro-atpA	3	AAAGCAGTTCGAACCGGAAT	CACAAAAACAGTTAGCGCGT	20	20	45.0	45.0	58.1	57.9	24.2	8.4	0.207424	68735	69202	1060-1060	0.500000	58.3
tRNA-Pro-atpA_p4	tRNA-Pro-atpA	4	AGGGGGCCAAAAAGTTTGAT	ACAAAAACAGTTAGCGCGTG	20	20	45.0	45.0	57.9	57.9	4.4	8.4	0.216502	68735	69202	1216-1216	0.500000	58.3
tRNA-Pro-atpA_p5	tRNA-Pro-atpA	5	AGGGGGCCAAAAAGTTTGAT	CACAAAAACAGTTAGCGCGT	20	20	45.0	45.0	57.9	57.9	4.4	8.4	0.216502	68735	69202	1217-1217	0.500000	58.3