primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	TATCCAATGATCCGGGACGT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.292527	7074	7144	185-185	1.000000	78.1
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TATCCAATGATCCGGGACGT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.293915	7074	7144	186-186	1.000000	78.1
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	TATCCAATGATCCGGGACGT	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.397392	7074	7144	204-204	1.000000	77.2
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TATCCAATGATCCGGGACGT	TTTTTCGTACCGAGGGTTCG	20	20	50.0	50.0	58.3	58.2	0.0	7.4	0.505900	7074	7144	148-148	1.000000	76.5
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TATCCAATGATCCGGGACGT	GACTAAAGCGTCGGATTGCT	20	20	50.0	50.0	58.3	58.4	0.0	2.3	0.646367	7074	7144	183-183	1.000000	75.3
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	20	20	45.0	50.0	58.0	57.9	1.4	4.5	0.171668	13396	14333	977-1014	1.000000	81.9
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	20	20	45.0	45.0	58.0	58.1	1.4	0.0	0.179004	13396	14333	989-1026	1.000000	81.8
atpH-atpI_p3	atpH-atpI	3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	20	20	50.0	50.0	57.9	57.9	16.1	4.5	0.213567	13396	14333	1007-1044	1.000000	81.5
atpH-atpI_p4	atpH-atpI	4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	20	20	50.0	45.0	57.9	58.1	16.1	0.0	0.220903	13396	14333	1019-1056	1.000000	81.4
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	20	20	45.0	50.0	58.2	57.9	1.4	4.5	0.317207	13396	14333	978-1015	1.000000	80.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	28208	28833	707-942	1.000000	77.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	CCTTTAGAGTCCACTTCGCC	20	20	50.0	55.0	58.4	58.0	14.7	0.0	0.367031	28208	28833	766-1001	1.000000	77.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	CTTTAGAGTCCACTTCGCCC	20	20	50.0	55.0	58.4	58.0	14.7	0.0	0.367031	28208	28833	765-1000	1.000000	77.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTTTGTTTGGCGACATGGC	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	58.4	58.0	0.0	0.0	0.429688	28208	28833	740-975	1.000000	76.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTTTGTTTGGCGACATGGC	CTTTAGAGTCCACTTCGCCC	20	20	45.0	55.0	58.4	58.0	0.0	0.0	0.435184	28208	28833	798-1033	1.000000	76.8
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	20	21	45.0	42.9	58.0	57.0	0.0	0.0	1.975331	28924	29298	508-530	1.000000	67.7
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.8	57.0	0.0	0.0	2.160415	28924	29298	514-536	1.000000	66.2
petN-psbM_p3	petN-psbM	3	TCTGTCCCTCGTAGTATGGG	TGCTACTGCACTCTTCATTCT	20	21	55.0	42.9	57.6	57.0	0.0	0.0	2.329269	28924	29298	469-491	1.000000	65.2
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	AAAACCGTGAGTCAAAGTGA	20	20	45.0	40.0	58.0	55.4	0.0	0.0	2.558137	28924	29298	453-475	1.000000	63.5
petN-psbM_p5	petN-psbM	5	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	20	20	45.0	45.0	58.0	55.5	0.0	0.0	2.521758	28924	29298	508-530	1.000000	63.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31002	31904	1016-1044	1.000000	83.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31002	31904	995-1023	1.000000	83.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31002	31904	992-1020	1.000000	83.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31002	31904	978-1006	1.000000	83.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31002	31904	957-985	1.000000	83.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AGGAGCCCCTTTAACTCAGT	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.0	58.0	0.0	5.0	0.049988	31977	33271	1404-1455	1.000000	82.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.067776	31977	33271	1386-1437	1.000000	82.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.089930	31977	33271	1389-1440	1.000000	82.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GAGCCCCTTTAACTCAGTGG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	57.9	58.0	0.0	5.0	0.119051	31977	33271	1402-1453	1.000000	82.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGAGCCCCTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	57.9	58.0	0.0	5.0	0.119051	31977	33271	1403-1454	1.000000	82.0
psbC_p1	psbC	1	TTCCCTGAGGAGGTTCTACC	TTTGAAGTCTGGCCTTGACC	20	20	55.0	50.0	57.8	58.0	0.0	20.5	0.268053	34317	35701	1499-1500	1.000000	78.8
psbC_p2	psbC	2	AGGTATTCGTGCATGGATGG	TTTGAAGTCTGGCCTTGACC	20	20	50.0	50.0	57.7	58.0	10.2	20.5	0.279587	34317	35701	1551-1552	1.000000	78.6
psbC_p3	psbC	3	TTCCCTGAGGAGGTTCTACC	GCCAGGGGAATCAAATGGAT	20	20	55.0	50.0	57.8	57.9	0.0	1.7	0.341907	34317	35701	1456-1457	1.000000	78.2
psbC_p4	psbC	4	AGGTATTCGTGCATGGATGG	GCCAGGGGAATCAAATGGAT	20	20	50.0	50.0	57.7	57.9	10.2	1.7	0.353442	34317	35701	1508-1509	1.000000	78.1
psbC_p5	psbC	5	TTCCCTGAGGAGGTTCTACC	ATGCCAGGGGAATCAAATGG	20	20	55.0	50.0	57.8	58.2	0.0	0.0	0.451989	34317	35701	1458-1459	1.000000	77.3
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	42537	43144	786-786	1.000000	79.1
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	42537	43144	768-768	1.000000	79.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TCACAAGGCGCTTCGAATAA	20	20	50.0	45.0	57.9	57.9	4.1	7.6	0.202844	42537	43144	708-708	1.000000	78.7
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGCTT	20	20	50.0	45.0	57.9	58.1	4.1	7.6	0.219123	42537	43144	715-715	1.000000	78.6
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	42537	43144	782-782	1.000000	77.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47145	47990	896-900	1.000000	79.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47145	47990	904-908	1.000000	78.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAGACAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.223577	47145	47990	947-951	1.000000	78.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGATTCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.245117	47145	47990	928-932	1.000000	78.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CCAAATAGACAAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	21.9	0.0	0.282650	47145	47990	952-956	1.000000	78.0
ndhC-trnC-ACA_p1	ndhC-trnC-ACA	1	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.7	57.9	0.0	0.0	0.327388	51513	52290	906-913	1.000000	78.2
ndhC-trnC-ACA_p2	ndhC-trnC-ACA	2	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.388416	51513	52290	895-902	1.000000	77.7
ndhC-trnC-ACA_p3	ndhC-trnC-ACA	3	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	57.7	58.3	0.0	22.8	0.556769	51513	52290	907-914	1.000000	76.3
ndhC-trnC-ACA_p4	ndhC-trnC-ACA	4	CGGGGCTAAAATTCCGGAAA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.5	57.9	23.1	0.0	0.610185	51513	52290	892-899	1.000000	75.9
ndhC-trnC-ACA_p5	ndhC-trnC-ACA	5	AATCGGGGCTAAAATTCCGG	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.3	58.3	0.0	22.8	0.617798	51513	52290	896-903	1.000000	75.9
accD-psaI_p1	accD-psaI	1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.3	58.0	0.0	26.9	1.728241	59683	60346	792-899	1.000000	70.5
accD-psaI_p2	accD-psaI	2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.3	58.0	0.0	26.9	1.728241	59683	60346	791-898	1.000000	70.5
accD-psaI_p3	accD-psaI	3	AAAGGGTGTTCTGAGTGAGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.3	57.9	0.0	26.8	1.804774	59683	60346	798-905	1.000000	69.8
accD-psaI_p4	accD-psaI	4	AAGGGTGTTCTGAGTGAGTT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.3	57.9	0.0	26.8	1.804774	59683	60346	797-904	1.000000	69.8
accD-psaI_p5	accD-psaI	5	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.1	58.0	0.0	26.9	1.960865	59683	60346	792-899	1.000000	68.6
petA-psbJ_p1	petA-psbJ	1	TCCGTGTCCAAGGTCTTTTG	ATACTCAGGGTTGGGCTCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.139344	63684	64121	579-579	1.000000	79.6
petA-psbJ_p2	petA-psbJ	2	CATTCCGTGTCCAAGGTCTT	ATACTCAGGGTTGGGCTCAT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.286141	63684	64121	582-582	1.000000	78.4
petA-psbJ_p3	petA-psbJ	3	TCCGTGTCCAAGGTCTTTTG	GTTCATACTCAGGGTTGGGC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.286769	63684	64121	583-583	1.000000	78.4
petA-psbJ_p4	petA-psbJ	4	TCCGTGTCCAAGGTCTTTTG	TTCATACTCAGGGTTGGGCT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.369381	63684	64121	582-582	1.000000	77.7
petA-psbJ_p5	petA-psbJ	5	CCGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.425585	63684	64121	578-578	1.000000	77.3
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GGGTTCGGCTCAGTCAATTA	20	20	45.0	50.0	58.0	57.6	0.0	0.0	0.413929	64929	66090	1342-1396	1.000000	80.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GGGTTCGGCTCAGTCAATTA	20	20	50.0	50.0	57.8	57.6	24.9	0.0	0.552933	64929	66090	1254-1308	1.000000	79.4
psbE-petL_p3	psbE-petL	3	GTTCTCCTGTGCTTCCAGAC	GGGTTCGGCTCAGTCAATTA	20	20	55.0	50.0	58.2	57.6	0.9	0.0	0.594521	64929	66090	1249-1303	1.000000	79.1
psbE-petL_p4	psbE-petL	4	TCTCCTGTGCTTCCAGACAT	GGGTTCGGCTCAGTCAATTA	20	20	50.0	50.0	58.4	57.6	0.9	0.0	0.747268	64929	66090	1247-1301	1.000000	77.9
psbE-petL_p5	psbE-petL	5	TTCTCCTGTGCTTCCAGACA	GGGTTCGGCTCAGTCAATTA	20	20	50.0	50.0	58.6	57.6	0.9	0.0	0.973006	64929	66090	1248-1302	1.000000	76.0
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GCTAAAGACCCGAACCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.111295	66954	67443	590-613	1.000000	82.4
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GCTAAAGACCCGAACCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.112638	66954	67443	589-612	1.000000	82.4
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GCTAAAGACCCGAACCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.119331	66954	67443	601-624	1.000000	82.3
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GCTAAAGACCCGAACCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.120664	66954	67443	600-623	1.000000	82.3
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GCTAAAGACCCGAACCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.120664	66954	67443	603-626	1.000000	82.3
ndhF-rpl32_p1	ndhF-rpl32	1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAACGCTG	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.535120	113938	114905	1102-1119	1.000000	77.6
ndhF-rpl32_p2	ndhF-rpl32	2	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAACGCTGT	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.536812	113938	114905	1101-1118	1.000000	77.6
ndhF-rpl32_p3	ndhF-rpl32	3	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAACGCTG	20	21	50.0	42.9	58.0	58.0	0.0	0.0	1.062058	113938	114905	1103-1120	1.000000	73.4
ndhF-rpl32_p4	ndhF-rpl32	4	TGGGAAGTGGAATGAGAGGT	TCAACGCTGTAAAATAGGCCT	20	21	50.0	42.9	58.0	57.7	0.0	11.8	1.388734	113938	114905	1091-1108	1.000000	70.8
ndhF-rpl32_p5	ndhF-rpl32	5	CCACATGCGTCGAAGATTTT	TGAAAAAGCCTTCAACGCTG	20	20	45.0	45.0	57.1	57.5	1.4	0.0	1.390450	113938	114905	1170-1187	1.000000	70.6
ndhG-ndhI_p1	ndhG-ndhI	1	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGT	20	20	45.0	45.0	57.4	57.3	0.0	0.0	1.239733	120047	120525	557-596	1.000000	75.5
ndhG-ndhI_p2	ndhG-ndhI	2	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGTT	20	21	45.0	42.9	57.4	58.0	0.0	0.0	1.575164	120047	120525	557-596	1.000000	72.8
ndhG-ndhI_p3	ndhG-ndhI	3	ATTGGTCCAGGCAAATCCAT	ATCAAATCGCATTAGGTCGGT	20	21	45.0	42.9	57.4	57.8	0.0	0.0	1.749737	120047	120525	558-597	1.000000	71.4
ndhG-ndhI_p4	ndhG-ndhI	4	TCATGTATTGGTCCAGGCAA	TCAAATCGCATTAGGTCGGT	20	20	45.0	45.0	56.8	57.3	0.0	0.0	1.875728	120047	120525	563-602	1.000000	70.4
ndhG-ndhI_p5	ndhG-ndhI	5	TATTGGTCCAGGCAAATCCA	TCAAATCGCATTAGGTCGGT	20	20	45.0	45.0	56.8	57.3	0.0	0.0	1.898133	120047	120525	558-597	1.000000	70.2
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	AGTTTGTGAAATCCAACGAAGA	CCTTTTGGTTCTCCCCGAAA	22	20	36.4	50.0	56.5	58.0	0.0	0.0	3.520629	127945	128064	263-263	1.000000	53.1
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	AGTTTGTGAAATCCAACGAAGA	CCTTCCTTTTGGTTCTCCCC	22	20	36.4	55.0	56.5	58.1	0.0	0.0	3.585604	127945	128064	267-267	1.000000	52.5
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	AGTTTGTGAAATCCAACGAAGA	TTTTGGTTCTCCCCGAAACC	22	20	36.4	50.0	56.5	58.3	0.0	0.0	3.815491	127945	128064	261-261	1.000000	50.7
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	AGTTTGTGAAATCCAACGAAGAA	CCTTTTGGTTCTCCCCGAAA	23	20	34.8	50.0	57.2	58.0	0.0	0.0	3.838593	127945	128064	263-263	1.000000	50.5
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	AGTTTGTGAAATCCAACGAAGAA	CCTTCCTTTTGGTTCTCCCC	23	20	34.8	55.0	57.2	58.1	0.0	0.0	3.903568	127945	128064	267-267	1.000000	50.0