primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-chlB_p1	matK-chlB	1	ATTTTGGTAACGGGCGCTAT	GACTTCCGAAGCTGAGTCAG	20	20	45.0	55.0	58.0	58.0	7.6	16.2	0.057571	1735	3173	1818-1827	1.000000	87.9
matK-chlB_p2	matK-chlB	2	CTCTAAAACGCGGCTACACT	GACTTCCGAAGCTGAGTCAG	20	20	50.0	55.0	58.0	58.0	12.5	16.2	0.018400	1735	3173	2043-2043	0.500000	59.9
matK-chlB_p3	matK-chlB	3	TCTCTAAAACGCGGCTACAC	GACTTCCGAAGCTGAGTCAG	20	20	50.0	55.0	58.0	58.0	12.5	16.2	0.023872	1735	3173	2044-2044	0.500000	59.8
matK-chlB_p4	matK-chlB	4	CTCTAAAACGCGGCTACACT	CGATACAACTCATGCAGCCT	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.062443	1735	3173	2502-2502	0.500000	59.5
matK-chlB_p5	matK-chlB	5	CTCTAAAACGCGGCTACACT	TGTTTCTCAAGCAGCTTGGT	20	20	50.0	45.0	58.0	57.9	12.5	30.7	0.064968	1735	3173	2577-2577	0.500000	59.5
psbK-psbI_p1	psbK-psbI	1	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGCA	20	22	50.0	36.4	58.6	55.6	16.6	9.2	5.009230	5523	5944	429-466	1.000000	52.0
psbK-psbI_p2	psbK-psbI	2	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGCAT	20	23	50.0	34.8	58.6	56.2	16.6	9.2	5.484001	5523	5944	429-466	1.000000	52.0
psbK-psbI_p3	psbK-psbI	3	TCTCCTAGCTTTTGTTTGGCA	ACAAATAGTTTGAGGGTAAGCA	21	22	42.9	36.4	57.8	55.6	0.0	9.2	5.591251	5523	5944	451-488	1.000000	52.0
psbK-psbI_p4	psbK-psbI	4	GCTGCAGTTAGCTTTCGCTA	CAAATAGTTTGAGGGTAAGCA	20	21	50.0	38.1	58.6	53.8	16.6	0.0	5.836045	5523	5944	428-465	1.000000	52.0
psbK-psbI_p5	psbK-psbI	5	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGC	20	21	50.0	38.1	58.6	53.8	16.6	9.2	5.839998	5523	5944	429-466	1.000000	52.0
psbI-ycf12_p1	psbI-ycf12	1	TGCCAAATTACCCGAAGCTT	CGTTACTAAGCAGTGCGACT	20	20	45.0	50.0	58.1	58.0	0.0	10.0	0.103586	6056	6777	1856-1856	0.500000	59.2
psbI-ycf12_p2	psbI-ycf12	2	TTGCCAAATTACCCGAAGCT	CGTTACTAAGCAGTGCGACT	20	20	45.0	50.0	58.1	58.0	0.0	10.0	0.103586	6056	6777	1857-1857	0.500000	59.2
psbI-ycf12_p3	psbI-ycf12	3	TGCCAAATTACCCGAAGCTT	TCGTTACTAAGCAGTGCGAC	20	20	45.0	50.0	58.1	58.0	0.0	10.0	0.108990	6056	6777	1857-1857	0.500000	59.1
psbI-ycf12_p4	psbI-ycf12	4	TTGCCAAATTACCCGAAGCT	TCGTTACTAAGCAGTGCGAC	20	20	45.0	50.0	58.1	58.0	0.0	10.0	0.108990	6056	6777	1858-1858	0.500000	59.1
psbI-ycf12_p5	psbI-ycf12	5	GTTTCGGACTGCCTCATCTT	CGTTACTAAGCAGTGCGACT	20	20	50.0	50.0	57.9	58.0	0.0	10.0	0.122063	6056	6777	1718-1718	0.500000	59.0
atpH-atpI_p1	atpH-atpI	1	TCCCTCCTGGAAAGTTTTGC	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	4.9	0.004976	11795	12473	1827-1831	1.000000	85.3
atpH-atpI_p2	atpH-atpI	2	TCCCTCCTGGAAAGTTTTGC	GAATACCGTTCCTGGGTTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.038728	11795	12473	1724-1728	1.000000	85.3
atpH-atpI_p3	atpH-atpI	3	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	20	20	55.0	45.0	58.0	58.0	9.6	4.9	0.024692	11795	12473	1866-1870	1.000000	85.0
atpH-atpI_p4	atpH-atpI	4	TCCCTCCTGGAAAGTTTTGC	TGGCAAGTAGGAGTCTTCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028799	11795	12473	1913-1917	1.000000	85.0
atpH-atpI_p5	atpH-atpI	5	TCCCTCCTGGAAAGTTTTGC	TGGGGGAAGAAGAATACCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043491	11795	12473	1735-1735	0.500000	59.7
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	CAAGTTCTGGTAGTACGGGC	AATGTCAGCCTAACGTGTCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.100733	24626	25269	1217-1217	0.500000	59.2
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	GCAAGTTCTGGTAGTACGGG	AATGTCAGCCTAACGTGTCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.100733	24626	25269	1218-1218	0.500000	59.2
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	CCCCTTCGAATTCATCCAGG	AATGTCAGCCTAACGTGTCC	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.121890	24626	25269	1504-1504	0.500000	59.0
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	CCCTTCGAATTCATCCAGGG	AATGTCAGCCTAACGTGTCC	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.121890	24626	25269	1503-1503	0.500000	59.0
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	GTAGCATCCTCCGACGATTC	AATGTCAGCCTAACGTGTCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.148044	24626	25269	1836-1836	0.500000	58.8
psbM-petN_p1	psbM-petN	1	TCTTTACATTCAGACGGCCG	TTTCATGTGTCTCGATGCGT	20	20	50.0	45.0	58.0	57.9	26.9	0.0	0.083431	26143	27120	1364-1402	1.000000	87.9
psbM-petN_p2	psbM-petN	2	TCTTTACATTCAGACGGCCG	AAGACGGAGGACTGCAAATC	20	20	50.0	50.0	58.0	57.9	26.9	0.0	0.105187	26143	27120	1568-1607	1.000000	87.2
psbM-petN_p3	psbM-petN	3	TCTTTACATTCAGACGGCCG	CCTTAATGCCCCAGTTCGAA	20	20	50.0	50.0	58.0	57.9	26.9	0.0	0.127366	26143	27120	1549-1588	1.000000	87.1
psbM-petN_p4	psbM-petN	4	TCTTTACATTCAGACGGCCG	GGTGTCGCCTAAAAGGAGAG	20	20	50.0	55.0	58.0	58.0	26.9	0.0	0.016123	26143	27120	1564-1564	0.500000	59.9
psbM-petN_p5	psbM-petN	5	GGACACGTTAGGCTGACATT	GGTGTCGCCTAAAAGGAGAG	20	20	50.0	55.0	57.9	58.0	10.2	0.0	0.106302	26143	27120	1872-1872	0.500000	59.1
trnC-GCA-trnG-GCC_p1	trnC-GCA-trnG-GCC	1	CTCTCCTTTTAGGCGACACC	GACGTTACGGGTGGAGTTAC	20	20	55.0	55.0	58.0	58.0	0.0	10.2	0.028928	27735	28220	868-868	0.500000	59.8
trnC-GCA-trnG-GCC_p2	trnC-GCA-trnG-GCC	2	CTCTCCTTTTAGGCGACACC	GGGGATTGGATGGTACGAAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.048368	27735	28220	1154-1154	0.500000	59.6
trnC-GCA-trnG-GCC_p3	trnC-GCA-trnG-GCC	3	CTCTCCTTTTAGGCGACACC	TTTCAGGGGCGTCATTATGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.062874	27735	28220	976-976	0.500000	59.5
trnC-GCA-trnG-GCC_p4	trnC-GCA-trnG-GCC	4	AGGCTACTAAAGGGACGGTT	GACGTTACGGGTGGAGTTAC	20	20	50.0	55.0	58.1	58.0	0.0	10.2	0.068997	27735	28220	1429-1429	0.500000	59.4
trnC-GCA-trnG-GCC_p5	trnC-GCA-trnG-GCC	5	AGGCTACTAAAGGGACGGTT	GGGGATTGGATGGTACGAAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.088437	27735	28220	1715-1715	0.500000	59.3
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.536534	31987	33346	1427-1445	1.000000	84.5
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	ACGGTCTCTTCTTAGCCAGT	AACTCAGGGGTAGAGTGACG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.526931	31987	33346	1487-1505	1.000000	84.2
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	57.9	58.5	8.1	0.0	0.595396	31987	33346	1426-1444	1.000000	84.0
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CAACCCACGAAGACAAAACG	AACTCAGGGGTAGAGTGACG	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.552673	31987	33346	1504-1522	1.000000	83.9
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ACCAACCCACGAAGACAAAA	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	57.9	58.5	0.0	0.0	0.584815	31987	33346	1506-1524	1.000000	83.7
petA-psbJ_p1	petA-psbJ	1	GCAGCTAGCAGAAATGAATT	GTTGGGGTCGTCTCTTCAAT	20	20	40.0	50.0	54.3	57.5	18.8	0.0	4.134486	57571	58251	713-728	1.000000	58.9
petA-psbJ_p2	petA-psbJ	2	GCAGCTAGCAGAAATGAATTT	GTTGGGGTCGTCTCTTCAAT	21	20	38.1	50.0	55.2	57.5	18.8	0.0	4.295888	57571	58251	713-728	1.000000	57.6
petA-psbJ_p3	petA-psbJ	3	GCAGCTAGCAGAAATGAATTTT	GTTGGGGTCGTCTCTTCAAT	22	20	36.4	50.0	55.9	57.5	18.8	0.0	4.531926	57571	58251	713-728	1.000000	55.7
petA-psbJ_p4	petA-psbJ	4	GCAGCTAGCAGAAATGAATTTTTG	GTTGGGGTCGTCTCTTCAAT	24	20	37.5	50.0	57.9	57.5	18.8	0.0	4.565533	57571	58251	713-728	1.000000	55.5
petA-psbJ_p5	petA-psbJ	5	GCAGCTAGCAGAAATGAATTTTT	GTTGGGGTCGTCTCTTCAAT	23	20	34.8	50.0	56.6	57.5	18.8	0.0	4.833059	57571	58251	713-728	1.000000	53.3
rrn16-rps12_p1	rrn16-rps12	1	TCGTTGCCCGGACTATATCT	AAAGGAGAATGAGCTCGCAG	20	20	50.0	50.0	58.0	58.0	0.7	9.6	0.030234	95112	95231	895-895	0.500000	59.8
rrn16-rps12_p2	rrn16-rps12	2	TTCTAGCGCCCAGGATATGA	AAAGGAGAATGAGCTCGCAG	20	20	50.0	50.0	58.0	58.0	2.3	9.6	0.031037	95112	95231	767-767	0.500000	59.8
rrn16-rps12_p3	rrn16-rps12	3	CACACTGTGACCTATTCGGG	AAAGGAGAATGAGCTCGCAG	20	20	55.0	50.0	58.0	58.0	15.9	9.6	0.031500	95112	95231	803-803	0.500000	59.7
rrn16-rps12_p4	rrn16-rps12	4	CCACACTGTGACCTATTCGG	AAAGGAGAATGAGCTCGCAG	20	20	55.0	50.0	58.0	58.0	15.9	9.6	0.031500	95112	95231	804-804	0.500000	59.7
rrn16-rps12_p5	rrn16-rps12	5	TCGTTGCCCGGACTATATCT	GGATCTACCTGCTCCCAGAT	20	20	50.0	55.0	58.0	58.0	0.7	0.0	0.031597	95112	95231	1036-1036	0.500000	59.7
trnP-GGG-trnL-UAG_p1	trnP-GGG-trnL-UAG	1	CGCTACCAAACTGTGCCATA	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.325734	113454	113704	338-346	1.000000	88.0
trnP-GGG-trnL-UAG_p2	trnP-GGG-trnL-UAG	2	TGAACCTGTGACCTCCATCA	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.330352	113454	113704	371-379	1.000000	87.7
trnP-GGG-trnL-UAG_p3	trnP-GGG-trnL-UAG	3	CGCTACCAAACTGTGCCATA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.391337	113454	113704	308-316	1.000000	87.7
trnP-GGG-trnL-UAG_p4	trnP-GGG-trnL-UAG	4	CGCTACCAAACTGTGCCATA	CAGCGTGTCTACCTGTTTCA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.415975	113454	113704	299-307	1.000000	87.6
trnP-GGG-trnL-UAG_p5	trnP-GGG-trnL-UAG	5	TGAACCTGTGACCTCCATCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.395954	113454	113704	341-349	1.000000	87.4
rps12-rrn16_p1	rps12-rrn16	1	AAAGGAGAATGAGCTCGCAG	TCGTTGCCCGGACTATATCT	20	20	50.0	50.0	58.0	58.0	9.6	0.7	0.030234	145211	145330	895-895	0.500000	59.8
rps12-rrn16_p2	rps12-rrn16	2	AAAGGAGAATGAGCTCGCAG	TTCTAGCGCCCAGGATATGA	20	20	50.0	50.0	58.0	58.0	9.6	2.3	0.031037	145211	145330	767-767	0.500000	59.8
rps12-rrn16_p3	rps12-rrn16	3	AAAGGAGAATGAGCTCGCAG	CACACTGTGACCTATTCGGG	20	20	50.0	55.0	58.0	58.0	9.6	15.9	0.031500	145211	145330	803-803	0.500000	59.7
rps12-rrn16_p4	rps12-rrn16	4	AAAGGAGAATGAGCTCGCAG	CCACACTGTGACCTATTCGG	20	20	50.0	55.0	58.0	58.0	9.6	15.9	0.031500	145211	145330	804-804	0.500000	59.7
rps12-rrn16_p5	rps12-rrn16	5	GGATCTACCTGCTCCCAGAT	TCGTTGCCCGGACTATATCT	20	20	55.0	50.0	58.0	58.0	0.0	0.7	0.031597	145211	145330	1036-1036	0.500000	59.7