primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf12-trnG-UCC_p1	ycf12-trnG-UCC	1	AATAGTTGCATCGGGACCAC	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.0	57.0	0.0	0.0	1.024344	9198	9346	230-234	1.000000	73.9
ycf12-trnG-UCC_p2	ycf12-trnG-UCC	2	AATTGCTCTATCGGCAGCTC	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.109635	9198	9346	206-210	1.000000	73.4
ycf12-trnG-UCC_p3	ycf12-trnG-UCC	3	TGCATCGGGACCACTAGTAA	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.130662	9198	9346	224-228	1.000000	73.1
ycf12-trnG-UCC_p4	ycf12-trnG-UCC	4	TTGCATCGGGACCACTAGTA	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.130662	9198	9346	225-229	1.000000	73.1
ycf12-trnG-UCC_p5	ycf12-trnG-UCC	5	TAGTTGCATCGGGACCACTA	TACCACTAAACCATACCCGC	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.130662	9198	9346	228-232	1.000000	73.0
trnE-UUC-trnY-GUA_p1	trnE-UUC-trnY-GUA	1	CTCTCTTTCAAGGAGGCGAC	CACGATGCCACAAACGAATC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.120998	27705	27857	314-314	1.000000	79.8
trnE-UUC-trnY-GUA_p2	trnE-UUC-trnY-GUA	2	CTCTCTTTCAAGGAGGCGAC	CGATGCCACAAACGAATCTG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.167022	27705	27857	312-312	1.000000	79.4
trnE-UUC-trnY-GUA_p3	trnE-UUC-trnY-GUA	3	CTCTCTTTCAAGGAGGCGAC	ACGATGCCACAAACGAATCT	20	20	55.0	45.0	58.0	58.2	0.0	0.0	0.194786	27705	27857	313-313	1.000000	79.2
trnE-UUC-trnY-GUA_p4	trnE-UUC-trnY-GUA	4	CACCTCTCTTTCAAGGAGGC	CACGATGCCACAAACGAATC	20	20	55.0	50.0	57.9	58.1	17.5	0.0	0.224279	27705	27857	317-317	1.000000	79.0
trnE-UUC-trnY-GUA_p5	trnE-UUC-trnY-GUA	5	CACCTCTCTTTCAAGGAGGC	CGATGCCACAAACGAATCTG	20	20	55.0	50.0	57.9	57.8	17.5	0.0	0.270303	27705	27857	315-315	1.000000	78.6
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	CTCATCAGCCAGGGGGTATA	GACGCCATATGCTCGATGAT	20	20	55.0	50.0	58.0	58.0	0.0	18.3	0.031839	30184	30303	1218-1227	1.000000	87.9
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TACCAGCTGCCTCCTCTATC	GACGCCATATGCTCGATGAT	20	20	55.0	50.0	58.0	58.0	8.7	18.3	0.004317	30184	30303	1075-1075	0.500000	60.0
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	CTATACCAGCTGCCTCCTCT	GACGCCATATGCTCGATGAT	20	20	55.0	50.0	58.0	58.0	8.7	18.3	0.010054	30184	30303	1078-1078	0.500000	59.9
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	GTCCCTACGTATGCTTGCAA	GACGCCATATGCTCGATGAT	20	20	50.0	50.0	58.0	58.0	0.0	18.3	0.020354	30184	30303	1491-1491	0.500000	59.8
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	TACCAGCTGCCTCCTCTATC	GGTGCTGGAGTGGTTGTTTA	20	20	55.0	50.0	58.0	58.0	8.7	0.0	0.026846	30184	30303	731-731	0.500000	59.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TCTTCTTAGCCAGTCGTCCA	TAAGCCGTCGGTTCAAATCC	20	20	50.0	50.0	58.1	58.3	0.0	13.9	0.351476	41328	42882	1661-1665	1.000000	78.0
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TCTTCTTAGCCAGTCGTCCA	GGCCCTTTTAACTCAGTGGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.368737	41328	42882	1703-1707	1.000000	77.8
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CGGACCAACCTACGAAAACA	TAAGCCGTCGGTTCAAATCC	20	20	50.0	50.0	58.1	58.3	0.0	13.9	0.402247	41328	42882	1689-1693	1.000000	77.5
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CGGACCAACCTACGAAAACA	GGCCCTTTTAACTCAGTGGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.419508	41328	42882	1731-1735	1.000000	77.4
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TCTTCTTAGCCAGTCGTCCA	AGGCCCTTTTAACTCAGTGG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.667408	41328	42882	1704-1708	1.000000	75.4
cemA_p1	cemA	1	AGAGAAAGTGGGGAGAGTCC	GGATAAGTCCAGCCCTTTAACA	20	22	55.0	45.5	58.1	57.8	0.0	0.0	2.285165	64877	66382	1641-1648	1.000000	62.8
cemA_p2	cemA	2	AGAAAGTGGGGAGAGTCCAA	GGATAAGTCCAGCCCTTTAACA	20	22	50.0	45.5	57.9	57.8	0.0	0.0	2.349148	64877	66382	1639-1646	1.000000	62.3
cemA_p3	cemA	3	CGGATTCCCTCCTCCTGATA	GGATAAGTCCAGCCCTTTAACA	20	22	55.0	45.5	57.7	57.8	0.0	0.0	2.541524	64877	66382	1572-1579	1.000000	60.8
cemA_p4	cemA	4	GGGGAGAGTCCAAAAGGGTA	GGATAAGTCCAGCCCTTTAACA	20	22	55.0	45.5	58.3	57.8	0.0	0.0	2.565608	64877	66382	1632-1639	1.000000	60.6
cemA_p5	cemA	5	GAGAAAGTGGGGAGAGTCCA	GGATAAGTCCAGCCCTTTAACA	20	22	55.0	45.5	58.4	57.8	0.0	0.0	2.585035	64877	66382	1640-1647	1.000000	60.4
rpl33-rps18_p1	rpl33-rps18	1	CAAGCACACCAACCACAAAG	GAACGTCTACGGGAAGATCG	20	20	50.0	55.0	58.1	57.9	0.0	7.5	0.177245	71775	71833	128-128	1.000000	79.8
rpl33-rps18_p2	rpl33-rps18	2	CAAGCACACCAACCACAAAG	TCTTCCCTGCTCACTTACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.155530	71775	71833	207-207	1.000000	79.5
rpl33-rps18_p3	rpl33-rps18	3	ACTACGCGTAAGAATCGTCG	TCTTCCCTGCTCACTTACGA	20	20	50.0	50.0	57.9	58.1	21.0	0.0	0.163493	71775	71833	275-275	1.000000	79.3
rpl33-rps18_p4	rpl33-rps18	4	CTACGCGTAAGAATCGTCGT	TCTTCCCTGCTCACTTACGA	20	20	50.0	50.0	57.9	58.1	21.0	0.0	0.163493	71775	71833	274-274	1.000000	79.3
rpl33-rps18_p5	rpl33-rps18	5	CTCGGTACACTACGCGTAAG	TCTTCCCTGCTCACTTACGA	20	20	55.0	50.0	57.9	58.1	21.0	0.0	0.163493	71775	71833	283-283	1.000000	79.3
psbT-psbN_p1	psbT-psbN	1	TCGAGAACCACCCAAAGTTC	AGAGACCCTTTCGAGGAACA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.260966	78505	78621	182-184	1.000000	81.4
psbT-psbN_p2	psbT-psbN	2	CCCATGGAAGCACTGGTTTA	AGAGACCCTTTCGAGGAACA	20	20	50.0	50.0	57.8	58.0	15.2	0.0	0.225498	78505	78621	253-255	1.000000	80.7
psbT-psbN_p3	psbT-psbN	3	TCTACGGTTGAACCCATGGA	AGAGACCCTTTCGAGGAACA	20	20	50.0	50.0	58.4	58.0	15.2	0.0	0.364210	78505	78621	265-267	1.000000	79.5
psbT-psbN_p4	psbT-psbN	4	CCCATGGAAGCACTGGTTTA	CTCAGAGACCCTTTCGAGGA	20	20	50.0	55.0	57.8	58.2	15.2	0.0	0.373639	78505	78621	256-258	1.000000	79.5
psbT-psbN_p5	psbT-psbN	5	GTTGAACCCATGGAAGCACT	AGAGACCCTTTCGAGGAACA	20	20	50.0	50.0	58.4	58.0	15.2	0.0	0.390300	78505	78621	259-261	1.000000	79.3
rpl22-rps19_p1	rpl22-rps19	1	TGATCGATAATCCGTCGCAC	ACTGATCGTATGGTGGGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.185642	88389	88405	202-202	1.000000	78.9
rpl22-rps19_p2	rpl22-rps19	2	TGATCGATAATCCGTCGCAC	TCGTATGGTGGGTCACAAAC	20	20	50.0	50.0	58.0	57.8	0.0	0.4	0.219134	88389	88405	197-197	1.000000	78.7
rpl22-rps19_p3	rpl22-rps19	3	TGATCGATAATCCGTCGCAC	CGTATGGTGGGTCACAAACT	20	20	50.0	50.0	58.0	57.8	0.0	0.4	0.225195	88389	88405	196-196	1.000000	78.6
rpl22-rps19_p4	rpl22-rps19	4	GTCGCACCTTAGTAGCTGAC	ACTGATCGTATGGTGGGTCA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.228671	88389	88405	189-189	1.000000	78.6
rpl22-rps19_p5	rpl22-rps19	5	CGTACGAACAACCCTGGATT	ACTGATCGTATGGTGGGTCA	20	20	50.0	50.0	57.9	58.1	10.4	0.0	0.228308	88389	88405	222-222	1.000000	78.5
rrn23_p1	rrn23	1	ACTTCTGTCGTTTCACGCTT	TCGTAGCATTCAACGACGAG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.172889	94765	97648	2927-2994	1.000000	83.0
rrn23_p2	rrn23	2	TTCTGCGTCTAGAGTCTCCC	TCGTAGCATTCAACGACGAG	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.356139	94765	97648	2872-2939	1.000000	81.6
rrn23_p3	rrn23	3	CGGCTTTTTCTGCGTCTAGA	TCGTAGCATTCAACGACGAG	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.398808	94765	97648	2879-2946	1.000000	81.2
rrn23_p4	rrn23	4	TTCTGCGTCTAGAGTCTCCC	AGCACGTTGAAGTGTCGTAG	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.480028	94765	97648	2886-2953	1.000000	80.6
rrn23_p5	rrn23	5	TTCTGCGTCTAGAGTCTCCC	AGTAGCACGTTGAAGTGTCG	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.480028	94765	97648	2889-2956	1.000000	80.6
rps12_p1	rps12	1	GAATGACGAGGAGCCGTATG	TTCTGTTCGCTACACTGCTC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.293810	103753	104583	1021-1021	1.000000	78.0
rps12_p2	rps12	2	GGTGGGAATCTCATGTACGG	TTCTGTTCGCTACACTGCTC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.306486	103753	104583	1000-1000	1.000000	77.9
rps12_p3	rps12	3	AATTGAATGACGAGGAGCCG	TTCTGTTCGCTACACTGCTC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.409340	103753	104583	1025-1025	1.000000	77.1
rps12_p4	rps12	4	AATGACGAGGAGCCGTATGA	TTCTGTTCGCTACACTGCTC	20	20	50.0	50.0	58.6	57.9	0.0	0.0	0.666279	103753	104583	1020-1020	1.000000	75.1
rps12_p5	rps12	5	TGAATGACGAGGAGCCGTAT	TTCTGTTCGCTACACTGCTC	20	20	50.0	50.0	58.6	57.9	0.0	0.0	0.666279	103753	104583	1022-1022	1.000000	75.1
rpl21-rpl32_p1	rpl21-rpl32	1	AATTTGGTCCGATTCGTGGT	GCTACTTTCACCGTTTTGGC	20	20	45.0	50.0	57.8	58.0	5.2	0.0	0.240484	118134	118326	339-345	1.000000	79.0
rpl21-rpl32_p2	rpl21-rpl32	2	ATTTGGTCCGATTCGTGGTC	GCTACTTTCACCGTTTTGGC	20	20	50.0	50.0	58.3	58.0	5.2	0.0	0.312120	118134	118326	338-344	1.000000	78.4
rpl21-rpl32_p3	rpl21-rpl32	3	AATTTGGTCCGATTCGTGGT	GGCCGAGGAAAAAGCTTTTG	20	20	45.0	50.0	57.8	58.2	5.2	24.5	0.410918	118134	118326	361-367	1.000000	77.6
rpl21-rpl32_p4	rpl21-rpl32	4	AATTTGGTCCGATTCGTGGT	CGTTTTGGCCCTCCATACAT	20	20	45.0	50.0	57.8	58.2	5.2	0.0	0.431494	118134	118326	328-334	1.000000	77.5
rpl21-rpl32_p5	rpl21-rpl32	5	ATTTGGTCCGATTCGTGGTC	GGCCGAGGAAAAAGCTTTTG	20	20	50.0	50.0	58.3	58.2	5.2	24.5	0.482553	118134	118326	360-366	1.000000	77.0
rrn23_p1	rrn23	1	TCGTAGCATTCAACGACGAG	ACTTCTGTCGTTTCACGCTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.172889	154374	157257	2927-2994	1.000000	83.0
rrn23_p2	rrn23	2	TCGTAGCATTCAACGACGAG	TTCTGCGTCTAGAGTCTCCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.356139	154374	157257	2872-2939	1.000000	81.6
rrn23_p3	rrn23	3	TCGTAGCATTCAACGACGAG	CGGCTTTTTCTGCGTCTAGA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.398808	154374	157257	2879-2946	1.000000	81.2
rrn23_p4	rrn23	4	AGCACGTTGAAGTGTCGTAG	TTCTGCGTCTAGAGTCTCCC	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.480028	154374	157257	2886-2953	1.000000	80.6
rrn23_p5	rrn23	5	AGTAGCACGTTGAAGTGTCG	TTCTGCGTCTAGAGTCTCCC	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.480028	154374	157257	2889-2956	1.000000	80.6