primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score ndhF_p1 ndhF 1 TGTTTCGATGCAACAACCAA TCCCCACTCTCTGGATCTAA 20 20 40.0 50.0 56.8 56.4 0.0 0.0 2.785811 117651 119882 2885-2885 0.500000 37.7 ndhF_p2 ndhF 2 TGTTTCGATGCAACAACCAA TCCCCACTCTCTGGATCTAAA 20 21 40.0 47.6 56.8 57.2 0.0 0.0 3.012051 117651 119882 2885-2885 0.500000 35.9 ndhF_p3 ndhF 3 ATGTTTCGATGCAACAACCA TCCCCACTCTCTGGATCTAA 20 20 40.0 50.0 56.5 56.4 5.3 0.0 3.013215 117651 119882 2886-2886 0.500000 35.9 ndhF_p4 ndhF 4 TGTTTCGATGCAACAACCAA ATCCCCACTCTCTGGATCTA 20 20 40.0 50.0 56.8 56.2 0.0 0.0 3.025576 117651 119882 2886-2886 0.500000 35.8 ndhF_p5 ndhF 5 TGTTTCGATGCAACAACCAA TATCCCCACTCTCTGGATCT 20 20 40.0 50.0 56.8 56.2 0.0 0.0 3.025576 117651 119882 2887-2887 0.500000 35.8 ndhF-rpl32_p1 ndhF-rpl32 1 GGGGAATTGGGCAGACTTAG AGCGTGTCTACCGATTTCAC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.066423 119883 120836 2344-2344 0.500000 59.5 ndhF-rpl32_p2 ndhF-rpl32 2 GGGGAATTGGGCAGACTTAG CAGCGTGTCTACCGATTTCA 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.067794 119883 120836 2345-2345 0.500000 59.5 ndhF-rpl32_p3 ndhF-rpl32 3 AAATAACAGGAAGGCGCACA AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.1 58.0 10.8 0.0 0.103543 119883 120836 2311-2311 0.500000 59.2 ndhF-rpl32_p4 ndhF-rpl32 4 AATAACAGGAAGGCGCACAA AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.1 58.0 10.8 0.0 0.103543 119883 120836 2310-2310 0.500000 59.2 ndhF-rpl32_p5 ndhF-rpl32 5 ATAACAGGAAGGCGCACAAA AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.1 58.0 10.8 0.0 0.103543 119883 120836 2309-2309 0.500000 59.2 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 ACGACCAAGTAACCGGGATA AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 58.1 58.0 6.3 0.0 0.156526 121005 121462 999-999 0.500000 58.7 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 ACGACCAAGTAACCGGGATA CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 58.1 58.0 6.3 0.0 0.157897 121005 121462 1000-1000 0.500000 58.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 ACGACCAAGTAACCGGGATA GCTGCATAGCCCAATACCAT 20 20 50.0 50.0 58.1 58.1 6.3 0.0 0.233555 121005 121462 1591-1591 0.500000 58.1 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 ACGACCAAGTAACCGGGATA ATCATGCCTTTTTCGGACGA 20 20 50.0 45.0 58.1 57.9 6.3 0.0 0.256646 121005 121462 1276-1276 0.500000 57.9 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 ACGACCAAGTAACCGGGATA TGCCTTTTTCGGACGAATCA 20 20 50.0 45.0 58.1 58.1 6.3 8.1 0.263136 121005 121462 1272-1272 0.500000 57.9 trnL-UAG-ccsA_p1 trnL-UAG-ccsA 1 GTGAAATCGGTAGACACGCT GCTGCATAGCCCAATACCAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.092689 121543 121631 612-612 0.500000 59.3 trnL-UAG-ccsA_p2 trnL-UAG-ccsA 2 TGAAATCGGTAGACACGCTG GCTGCATAGCCCAATACCAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.094060 121543 121631 611-611 0.500000 59.2 trnL-UAG-ccsA_p3 trnL-UAG-ccsA 3 GTGAAATCGGTAGACACGCT ATCATGCCTTTTTCGGACGA 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.115780 121543 121631 297-297 0.500000 59.1 trnL-UAG-ccsA_p4 trnL-UAG-ccsA 4 TGAAATCGGTAGACACGCTG ATCATGCCTTTTTCGGACGA 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.117151 121543 121631 296-296 0.500000 59.1 trnL-UAG-ccsA_p5 trnL-UAG-ccsA 5 GTGAAATCGGTAGACACGCT TGCCTTTTTCGGACGAATCA 20 20 50.0 45.0 58.0 58.1 0.0 8.1 0.122270 121543 121631 293-293 0.500000 59.0 ccsA_p1 ccsA 1 GTGAAATCGGTAGACACGCT GGTGTTTGTGAGAACCCCTT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.075196 121632 122603 1585-1585 0.500000 59.4 ccsA_p2 ccsA 2 TGAAATCGGTAGACACGCTG GGTGTTTGTGAGAACCCCTT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.076566 121632 122603 1584-1584 0.500000 59.4 ccsA_p3 ccsA 3 GTGAAATCGGTAGACACGCT TGGGCCCCGAGAGTTATTTA 20 20 50.0 50.0 58.0 58.1 0.0 22.3 0.125522 121632 122603 1866-1866 0.500000 59.0 ccsA_p4 ccsA 4 TGAAATCGGTAGACACGCTG TGGGCCCCGAGAGTTATTTA 20 20 50.0 50.0 58.0 58.1 0.0 22.3 0.126892 121632 122603 1865-1865 0.500000 59.0 ccsA_p5 ccsA 5 GTGAAATCGGTAGACACGCT CAGTTGACAAGGTCGAAGCT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.136750 121632 122603 1780-1780 0.500000 58.9 ndhD_p1 ndhD 1 AAACACCAAACGTATCCGGT TGATCGTGTTTGCGAAAACG 20 20 45.0 45.0 57.7 58.0 0.0 21.4 0.271198 123211 124707 2212-2212 0.500000 57.8 ndhD_p2 ndhD 2 AAACACCAAACGTATCCGGT GATCGTGTTTGCGAAAACGT 20 20 45.0 45.0 57.7 58.0 0.0 25.1 0.272552 123211 124707 2211-2211 0.500000 57.8 ndhD_p3 ndhD 3 AAACACCAAACGTATCCGGT ACGTATGATCGTGTTTGCGA 20 20 45.0 45.0 57.7 58.0 0.0 0.0 0.273932 123211 124707 2217-2217 0.500000 57.8 ndhD_p4 ndhD 4 AAACACCAAACGTATCCGGT TCTGCTCCAAGAACAGAGGA 20 20 45.0 50.0 57.7 58.0 0.0 0.0 0.274723 123211 124707 1910-1910 0.500000 57.8 ndhD_p5 ndhD 5 AAACACCAAACGTATCCGGT AAACAACTCGAAGCATGGGT 20 20 45.0 45.0 57.7 58.0 0.0 0.0 0.278010 123211 124707 1816-1816 0.500000 57.8 psaC_p1 psaC 1 TTTCTTTCCACCCCACATGG TGATCGTGTTTGCGAAAACG 20 20 50.0 45.0 58.0 58.0 0.0 21.4 0.020174 124834 125079 1031-1031 0.500000 59.8 psaC_p2 psaC 2 TTTCTTTCCACCCCACATGG GATCGTGTTTGCGAAAACGT 20 20 50.0 45.0 58.0 58.0 0.0 25.1 0.021528 124834 125079 1030-1030 0.500000 59.8 psaC_p3 psaC 3 TTTCTTTCCACCCCACATGG ACGTATGATCGTGTTTGCGA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.022908 124834 125079 1036-1036 0.500000 59.8 psaC_p4 psaC 4 CCCGTCAGTAAAATAGGGCC TGATCGTGTTTGCGAAAACG 20 20 55.0 45.0 58.0 58.0 0.0 21.4 0.048760 124834 125079 835-835 0.500000 59.6 psaC_p5 psaC 5 CCCGTCAGTAAAATAGGGCC GATCGTGTTTGCGAAAACGT 20 20 55.0 45.0 58.0 58.0 0.0 25.1 0.050113 124834 125079 834-834 0.500000 59.6 psaC-ndhE_p1 psaC-ndhE 1 TCCTCTGTTCTTGGAGCAGA ACCGGCTATTGTTTCGTCAA 20 20 50.0 45.0 58.0 57.8 12.1 0.0 0.188307 125080 125339 476-476 0.500000 58.5 psaC-ndhE_p2 psaC-ndhE 2 ACCCATGCTTCGAGTTGTTT ACCGGCTATTGTTTCGTCAA 20 20 45.0 45.0 58.0 57.8 0.0 0.0 0.191595 125080 125339 570-570 0.500000 58.5 psaC-ndhE_p3 psaC-ndhE 3 GCTTCGAGTTGTTTCATGCC ACCGGCTATTGTTTCGTCAA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.203748 125080 125339 564-564 0.500000 58.4 psaC-ndhE_p4 psaC-ndhE 4 CCCGTCAGTAAAATAGGGCC ACCGGCTATTGTTTCGTCAA 20 20 55.0 45.0 58.0 57.8 0.0 0.0 0.209679 125080 125339 989-989 0.500000 58.3 psaC-ndhE_p5 psaC-ndhE 5 TCCTCTGTTCTTGGAGCAGA AGCTGCTTTGATAGGTGCAA 20 20 50.0 45.0 58.0 57.8 12.1 0.0 0.220479 125080 125339 985-985 0.500000 58.2 ndhG_p1 ndhG 1 CGTTTTCGCAAACACGATCA GATTACCTTAGCCCACGCAA 20 20 45.0 50.0 58.0 58.0 11.2 0.0 0.044612 125885 126415 1819-1819 0.500000 59.6 ndhG_p2 ndhG 2 CGTTTTCGCAAACACGATCA TACCTTAGCCCACGCAAATC 20 20 45.0 50.0 58.0 58.0 11.2 0.0 0.044612 125885 126415 1816-1816 0.500000 59.6 ndhG_p3 ndhG 3 CGTTTTCGCAAACACGATCA AACAAACAGTACGAGCTGCA 20 20 45.0 45.0 58.0 58.1 11.2 1.0 0.063828 125885 126415 1862-1862 0.500000 59.5 ndhG_p4 ndhG 4 CGTTTTCGCAAACACGATCA ACAAACAGTACGAGCTGCAA 20 20 45.0 45.0 58.0 58.1 11.2 1.0 0.063828 125885 126415 1861-1861 0.500000 59.5 ndhG_p5 ndhG 5 CGTTTTCGCAAACACGATCA AATCAAATTGCTTTGGGCCG 20 20 45.0 45.0 58.0 57.9 11.2 0.0 0.109612 125885 126415 1488-1488 0.500000 59.1 ndhI_p1 ndhI 1 GTGCTGCAGCTACAAAATGG GGGGATGGAATTTATGGCGT 20 20 50.0 50.0 58.0 58.0 36.6 0.0 0.017926 126655 127140 1471-1471 0.500000 59.9 ndhI_p2 ndhI 2 GTGCTGCAGCTACAAAATGG TGGGGATGGAATTTATGGCG 20 20 50.0 50.0 58.0 58.0 36.6 0.0 0.019346 126655 127140 1472-1472 0.500000 59.8 ndhI_p3 ndhI 3 CAAGAACCAGTCCCAACGAA GGGGATGGAATTTATGGCGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.042830 126655 127140 1417-1417 0.500000 59.7 ndhI_p4 ndhI 4 CAAGAACCAGTCCCAACGAA TGGGGATGGAATTTATGGCG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.044251 126655 127140 1418-1418 0.500000 59.6 ndhI_p5 ndhI 5 TCCCAACGAAAAGGCAGAAA GGGGATGGAATTTATGGCGT 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.064674 126655 127140 1407-1407 0.500000 59.5 ndhA_p1 ndhA 1 GATTTGCGTGGGCTAAGGTA GTGAGAGTTGAAGCCCCAAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.051424 127221 129769 2934-2934 0.500000 59.6 ndhA_p2 ndhA 2 TTGCGTGGGCTAAGGTAATC GTGAGAGTTGAAGCCCCAAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.051424 127221 129769 2931-2931 0.500000 59.6 ndhA_p3 ndhA 3 TGCAGCTCGTACTGTTTGTT GTGAGAGTTGAAGCCCCAAA 20 20 45.0 50.0 58.1 58.0 8.8 0.0 0.070639 127221 129769 2888-2888 0.500000 59.4 ndhA_p4 ndhA 4 TTGCAGCTCGTACTGTTTGT GTGAGAGTTGAAGCCCCAAA 20 20 45.0 50.0 58.1 58.0 8.8 0.0 0.070639 127221 129769 2889-2889 0.500000 59.4 ndhA_p5 ndhA 5 ACCAATGTACCTTGCAGCTC GTGAGAGTTGAAGCCCCAAA 20 20 50.0 50.0 58.2 58.0 5.4 0.0 0.192359 127221 129769 2900-2900 0.500000 58.5 ndhH_p1 ndhH 1 TCAGGTCCAATACGCTGTTG GAAAACCCGGGATCGATTGA 20 20 50.0 50.0 57.9 58.0 0.0 32.0 0.125987 129771 130952 1705-1705 0.500000 59.0 ndhH_p2 ndhH 2 TCAGGTCCAATACGCTGTTG AGAAAACCCGGGATCGATTG 20 20 50.0 50.0 57.9 58.0 0.0 32.0 0.131693 129771 130952 1706-1706 0.500000 58.9 ndhH_p3 ndhH 3 TCAGGTCCAATACGCTGTTG AAGAAGAAAACCCGGGATCG 20 20 50.0 50.0 57.9 57.9 0.0 32.0 0.202731 129771 130952 1710-1710 0.500000 58.4 ndhH_p4 ndhH 4 TCAGGTCCAATACGCTGTTG CAGTTGGATATTCGGGAGCC 20 20 50.0 55.0 57.9 58.1 0.0 0.0 0.209071 129771 130952 1513-1513 0.500000 58.3 ndhH_p5 ndhH 5 GAAGAACTCCCAAAGGACCG GAAAACCCGGGATCGATTGA 20 20 55.0 50.0 58.2 58.0 0.0 32.0 0.222997 129771 130952 1731-1731 0.500000 58.2 rps15_p1 rps15 1 ACTCCCTCAACCCGTTCTAA GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.038349 131056 131328 1456-1456 0.500000 59.7 rps15_p2 rps15 2 CAATGCCTACTCCCTCAACC GCAGCCGTTTCAGTATGGTA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.062665 131056 131328 1464-1464 0.500000 59.5 rps15_p3 rps15 3 CCAACAATGCCTACTCCCTC GCAGCCGTTTCAGTATGGTA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.062665 131056 131328 1468-1468 0.500000 59.5 rps15_p4 rps15 4 TCCTAACTGTTCCGGTCCAT GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.071557 131056 131328 1109-1109 0.500000 59.4 rps15_p5 rps15 5 TGCATTGATGGATGGTGAGG GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.080051 131056 131328 907-907 0.500000 59.4 rps15-ycf1_p1 rps15-ycf1 1 TCAATCGATCCCGGGTTTTC GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 58.0 58.0 22.9 0.0 0.051222 131329 131735 531-531 0.500000 59.6 rps15-ycf1_p2 rps15-ycf1 2 CAATCGATCCCGGGTTTTCT GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 58.0 58.0 22.9 0.0 0.056929 131329 131735 530-530 0.500000 59.5 rps15-ycf1_p3 rps15-ycf1 3 TCCTAACTGTTCCGGTCCAT GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.071557 131329 131735 1109-1109 0.500000 59.4 rps15-ycf1_p4 rps15-ycf1 4 TGCATTGATGGATGGTGAGG GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.080051 131329 131735 907-907 0.500000 59.4 rps15-ycf1_p5 rps15-ycf1 5 CTTGTAGCTGGGGCATTCAT GCAGCCGTTTCAGTATGGTA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.081512 131329 131735 859-859 0.500000 59.3 ycf1_p1 ycf1 1 TTACAGAATTGGGAGCGCTC GTCTGAAACCTTGGCACAGA 20 20 50.0 50.0 58.0 58.0 29.4 0.0 0.056857 133901 134020 1158-1158 0.500000 59.5 ycf1_p2 ycf1 2 TTACAGAATTGGGAGCGCTC TGCACCTATAATGGCGTTCC 20 20 50.0 50.0 58.0 58.0 29.4 0.0 0.062382 133901 134020 1249-1249 0.500000 59.5 ycf1_p3 ycf1 3 TTACAGAATTGGGAGCGCTC GCTACGATCCCCTCAAAAGG 20 20 50.0 55.0 58.0 58.0 29.4 0.0 0.069249 133901 134020 1127-1127 0.500000 59.4 ycf1_p4 ycf1 4 TTACAGAATTGGGAGCGCTC TTTCGTCTGAAACCTTGGCA 20 20 50.0 45.0 58.0 58.0 29.4 0.0 0.069737 133901 134020 1162-1162 0.500000 59.4 ycf1_p5 ycf1 5 TTACAGAATTGGGAGCGCTC CGAAACCGACGTTCGTTTTT 20 20 50.0 45.0 58.0 57.9 29.4 13.2 0.099940 133901 134020 1012-1012 0.500000 59.2