primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AGTCGCACTTAAAAGCCGAG	AGGTAATGCACGCAGCTAAA	20	20	50.0	45.0	58.6	57.9	6.4	0.0	0.678487	4109	4901	669-871	1.000000	79.4
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	TAGTCGCACTTAAAAGCCGA	AGGTAATGCACGCAGCTAAA	20	20	45.0	45.0	57.3	57.9	3.0	0.0	0.836640	4109	4901	670-872	1.000000	78.2
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TCGCACTTAAAAGCCGAGTA	AGGTAATGCACGCAGCTAAA	20	20	45.0	45.0	57.3	57.9	3.0	0.0	0.836640	4109	4901	667-869	1.000000	78.2
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	AGTCGCACTTAAAAGCCGAG	CACGACGAAAGGGCATTTTG	20	20	50.0	50.0	58.6	58.3	6.4	10.2	0.885235	4109	4901	702-904	1.000000	77.7
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	CGCACTTAAAAGCCGAGTAC	AGGTAATGCACGCAGCTAAA	20	20	50.0	45.0	57.2	57.9	0.0	0.0	0.890148	4109	4901	666-868	1.000000	77.7
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	GTTCGGTCACTATTGCGGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.0	0.120162	7255	7742	701-706	1.000000	81.9
psbK-psbI_p2	psbK-psbI	2	TCAAACTTGCCGAAGAGCTT	TTCTCTTCTTCATCTGCCGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.048417	7255	7742	1387-1398	1.000000	81.7
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTCTCTTCTTCATCTGCCGC	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.071830	7255	7742	1148-1159	1.000000	81.7
psbK-psbI_p4	psbK-psbI	4	TCAAACTTGCCGAAGAGCTT	GTTCGGTCACTATTGCGGAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.096749	7255	7742	940-948	1.000000	81.6
psbK-psbI_p5	psbK-psbI	5	GAATCATCCCTTCCAACCCC	TTCTCTTCTTCATCTGCCGC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.088988	7255	7742	1415-1426	1.000000	81.3
psbJ-petA_p1	psbJ-petA	1	GGGAGTAAGAGAAATGGCCG	TGTGGGTGGATTTGGTCAAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.051117	15399	16444	1333-1347	1.000000	81.5
psbJ-petA_p2	psbJ-petA	2	TGGAAGGATCCCTCTTTGGT	TGTGGGTGGATTTGGTCAAG	20	20	50.0	50.0	57.9	58.0	8.6	0.0	0.065800	15399	16444	1306-1320	1.000000	81.4
psbJ-petA_p3	psbJ-petA	3	TGGCTTATGATGTGTTCGGG	TGTGGGTGGATTTGGTCAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043002	15399	16444	1845-1859	1.000000	81.2
psbJ-petA_p4	psbJ-petA	4	AATGCAGTTCATCCAGCGAT	TGTGGGTGGATTTGGTCAAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054488	15399	16444	1610-1624	1.000000	81.2
psbJ-petA_p5	psbJ-petA	5	CCGGCCGAATGAGTATTTCA	TGTGGGTGGATTTGGTCAAG	20	20	50.0	50.0	58.1	58.0	30.7	0.0	0.057940	15399	16444	1820-1834	1.000000	81.1
ycf4-psaI_p1	ycf4-psaI	1	CCAGAAAGAGGATACAAGCCC	CGGCTTATTACTTCCGGCAA	21	20	52.4	50.0	58.1	58.3	0.0	0.0	1.416585	19806	20371	727-727	1.000000	70.8
ycf4-psaI_p2	ycf4-psaI	2	CCCAGAAAGAGGATACAAGCC	CGGCTTATTACTTCCGGCAA	21	20	52.4	50.0	58.1	58.3	0.0	0.0	1.416585	19806	20371	728-728	1.000000	70.8
ycf4-psaI_p3	ycf4-psaI	3	CCAGAAAGAGGATACAAGCCC	GTCGGCTTATTACTTCCGGC	21	20	52.4	55.0	58.1	58.8	0.0	0.0	1.860334	19806	20371	729-729	1.000000	67.2
ycf4-psaI_p4	ycf4-psaI	4	CCCAGAAAGAGGATACAAGCC	GTCGGCTTATTACTTCCGGC	21	20	52.4	55.0	58.1	58.8	0.0	0.0	1.860334	19806	20371	730-730	1.000000	67.2
ycf4-psaI_p5	ycf4-psaI	5	CCTAGTGAACCCAGAAAGAGG	CGGCTTATTACTTCCGGCAA	21	20	52.4	50.0	57.4	58.3	0.0	0.0	1.959697	19806	20371	737-737	1.000000	66.4
psaI-accD_p1	psaI-accD	1	TTGCCGGAAGTAATAAGCCG	TTGATCTGATCGTTCCACGT	20	20	50.0	45.0	58.3	57.0	5.0	12.7	1.371157	20483	21032	675-717	1.000000	74.5
psaI-accD_p2	psaI-accD	2	TTGCCGGAAGTAATAAGCCG	CAGCTTTACAGTCTTCCTCGT	20	21	50.0	47.6	58.3	57.7	5.0	0.0	1.646404	20483	21032	622-664	1.000000	72.4
psaI-accD_p3	psaI-accD	3	TTGCCGGAAGTAATAAGCCG	TTTGATCTGATCGTTCCACGT	20	21	50.0	42.9	58.3	57.7	5.0	12.7	1.654019	20483	21032	676-718	1.000000	72.3
psaI-accD_p4	psaI-accD	4	GCCGGAAGTAATAAGCCGAC	TTGATCTGATCGTTCCACGT	20	20	55.0	45.0	58.8	57.0	5.0	12.7	1.814906	20483	21032	673-715	1.000000	71.0
psaI-accD_p5	psaI-accD	5	CCGGAAGTAATAAGCCGACT	TTGATCTGATCGTTCCACGT	20	20	50.0	45.0	57.1	57.0	0.0	12.7	1.915044	20483	21032	672-714	1.000000	70.2
trnV-UAC-trnH-GUG_p1	trnV-UAC-trnH-GUG	1	TGCATGTTGGGTTCTTGGAA	TTCACAATCCACTGCCTTGG	20	20	45.0	50.0	57.9	58.4	0.0	0.0	0.459515	27688	30373	2426-2836	1.000000	81.7
trnV-UAC-trnH-GUG_p2	trnV-UAC-trnH-GUG	2	GCCCAATGCGATAAAATGCA	TTCACAATCCACTGCCTTGG	20	20	45.0	50.0	57.8	58.4	0.0	0.0	0.602353	27688	30373	2442-2852	1.000000	80.5
trnV-UAC-trnH-GUG_p3	trnV-UAC-trnH-GUG	3	ATGCATGTTGGGTTCTTGGA	TTCACAATCCACTGCCTTGG	20	20	45.0	50.0	57.7	58.4	0.0	0.0	0.687842	27688	30373	2427-2837	1.000000	79.9
trnV-UAC-trnH-GUG_p4	trnV-UAC-trnH-GUG	4	GCATGTTGGGTTCTTGGAAC	TTCACAATCCACTGCCTTGG	20	20	50.0	50.0	57.6	58.4	0.0	0.0	0.819214	27688	30373	2425-2835	1.000000	78.8
trnV-UAC-trnH-GUG_p5	trnV-UAC-trnH-GUG	5	TGCATGTTGGGTTCTTGGAA	CACAATCCACTGCCTTGGT	20	19	45.0	52.6	57.9	58.0	0.0	0.8	1.120135	27688	30373	2424-2834	1.000000	76.4
trnT-GGU-psaM_p1	trnT-GGU-psaM	1	GAACCGATGACTTACGCCTT	GCCAGGCAGGTCAGAAAAA	20	19	50.0	52.6	58.0	58.3	0.0	6.5	1.273682	30644	31904	1393-1436	1.000000	74.2
trnT-GGU-psaM_p2	trnT-GGU-psaM	2	TACCGCTGAGTTAAAAGGGC	GCCAGGCAGGTCAGAAAAA	20	19	50.0	52.6	57.9	58.3	0.0	6.5	1.371248	30644	31904	1358-1401	1.000000	73.6
trnT-GGU-psaM_p3	trnT-GGU-psaM	3	CCGATGACTTACGCCTTACC	GCCAGGCAGGTCAGAAAAA	20	19	55.0	52.6	58.2	58.3	0.0	6.5	1.418614	30644	31904	1390-1433	1.000000	73.1
trnT-GGU-psaM_p4	trnT-GGU-psaM	4	TTGAACCGATGACTTACGCC	GCCAGGCAGGTCAGAAAAA	20	19	50.0	52.6	58.3	58.3	0.0	6.5	1.550517	30644	31904	1395-1438	1.000000	72.0
trnT-GGU-psaM_p5	trnT-GGU-psaM	5	CGATGACTTACGCCTTACCA	GCCAGGCAGGTCAGAAAAA	20	19	50.0	52.6	57.4	58.3	0.0	6.5	1.839902	30644	31904	1389-1432	1.000000	69.7
ycf12-psbB_p1	ycf12-psbB	1	CCGTCAGAGTCAGAACAGTG	TATGAACGCGATACCAAGGC	20	20	55.0	50.0	57.9	58.1	0.0	15.9	0.196827	53571	54251	755-882	1.000000	83.6
ycf12-psbB_p2	ycf12-psbB	2	CCGTCAGAGTCAGAACAGTG	CAGAAATTAACCGGCCAGGA	20	20	55.0	50.0	57.9	57.9	0.0	18.7	0.180286	53571	54251	791-918	1.000000	83.5
ycf12-psbB_p3	ycf12-psbB	3	CCGTCAGAGTCAGAACAGTG	CGGTATGAACGCGATACCAA	20	20	55.0	50.0	57.9	58.2	0.0	26.0	0.214783	53571	54251	758-885	1.000000	83.4
ycf12-psbB_p4	ycf12-psbB	4	TGAGCTGCGCAATAACTTCT	CAGAAATTAACCGGCCAGGA	20	20	45.0	50.0	57.9	57.9	13.1	18.7	0.221925	53571	54251	773-900	1.000000	83.2
ycf12-psbB_p5	ycf12-psbB	5	CTAATGGGCCCGATACAACC	CAGAAATTAACCGGCCAGGA	20	20	55.0	50.0	58.1	57.9	19.4	18.7	0.230574	53571	54251	809-936	1.000000	83.0
trnI-CAU-trnF-GAA_p1	trnI-CAU-trnF-GAA	1	GTTGGGCGCTTTAACCATTC	AGGACTGAAAATCCTCGTGC	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.109805	67827	69662	1914-1919	1.000000	80.5
trnI-CAU-trnF-GAA_p2	trnI-CAU-trnF-GAA	2	GTTGGGCGCTTTAACCATTC	GACTGAAAATCCTCGTGCCA	20	20	50.0	50.0	58.0	58.2	7.6	0.0	0.200790	67827	69662	1912-1917	1.000000	79.8
trnI-CAU-trnF-GAA_p3	trnI-CAU-trnF-GAA	3	GTTGGGCGCTTTAACCATTC	ACCCTGTACCTGGATGATCC	20	20	50.0	55.0	58.0	58.2	7.6	4.0	0.201387	67827	69662	1971-1976	1.000000	79.7
trnI-CAU-trnF-GAA_p4	trnI-CAU-trnF-GAA	4	GTTGGGCGCTTTAACCATTC	CCCTGTACCTGGATGATCCA	20	20	50.0	55.0	58.0	58.2	7.6	14.4	0.202705	67827	69662	1970-1975	1.000000	79.7
trnI-CAU-trnF-GAA_p5	trnI-CAU-trnF-GAA	5	CCCGCGAGTTCACCAATTAT	AGGACTGAAAATCCTCGTGC	20	20	50.0	50.0	58.3	57.9	8.2	0.0	0.436910	67827	69662	1936-1941	1.000000	77.9
ycf3-psaA_p1	ycf3-psaA	1	CTCCGGGCAATTACATCGAA	GGCCTTTAGCTAGCGTCTTT	20	20	50.0	50.0	58.1	58.0	0.0	9.5	0.077484	75069	75692	798-808	1.000000	81.4
ycf3-psaA_p2	ycf3-psaA	2	TCCGGGCAATTACATCGAAG	GGCCTTTAGCTAGCGTCTTT	20	20	50.0	50.0	58.1	58.0	0.0	9.5	0.077484	75069	75692	797-807	1.000000	81.4
ycf3-psaA_p3	ycf3-psaA	3	CAGGACGCTTTGGAGAATGA	GGCCTTTAGCTAGCGTCTTT	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.124730	75069	75692	756-766	1.000000	81.1
ycf3-psaA_p4	ycf3-psaA	4	GCTGCTGAGTATTGGGAACA	GGCCTTTAGCTAGCGTCTTT	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.139449	75069	75692	832-842	1.000000	80.8
ycf3-psaA_p5	ycf3-psaA	5	ACAGGACGCTTTGGAGAATG	GGCCTTTAGCTAGCGTCTTT	20	20	50.0	50.0	58.2	58.0	0.0	9.5	0.212075	75069	75692	757-767	1.000000	80.4
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CCCACAAAAACGAAACGGTC	GGTTCAAGTCCGGTAAAGGG	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.352556	84451	85763	1420-1445	1.000000	79.5
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CCCACAAAAACGAAACGGTC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	84451	85763	1433-1458	1.000000	78.7
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	AACCCACAAAAACGAAACGG	GGTTCAAGTCCGGTAAAGGG	20	20	45.0	55.0	57.7	58.2	0.0	0.0	0.463783	84451	85763	1422-1447	1.000000	78.6
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CCCACAAAAACGAAACGGTC	CATCGGTTCAAGTCCGGTAA	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.543454	84451	85763	1424-1449	1.000000	77.9
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	CGTAGCCAGTCATCAACAGT	GGTTCAAGTCCGGTAAAGGG	20	20	50.0	55.0	57.6	58.2	0.0	0.0	0.552924	84451	85763	1396-1421	1.000000	77.9
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	GGGACTCTTGGGAACGAATC	TCTAACCCTCCGTTGTTCCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.045396	93832	94571	928-933	1.000000	80.9
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GGGACTCTTGGGAACGAATC	GCTGTTAACTGACTGGTCGT	20	20	55.0	50.0	58.0	57.8	0.0	15.9	0.175986	93832	94571	883-888	1.000000	79.9
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	GGGACTCTTGGGAACGAATC	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	58.0	58.2	0.0	18.9	0.182220	93832	94571	870-875	1.000000	79.9
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	GGGACTCTTGGGAACGAATC	CTGGTCGTAGGTTCGAATCC	20	20	55.0	55.0	58.0	57.8	0.0	4.4	0.230895	93832	94571	871-876	1.000000	79.5
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TCTAACCCTCCGTTGTTCCT	20	20	55.0	50.0	57.8	58.0	18.9	0.0	0.235714	93832	94571	885-890	1.000000	79.5
ycf1_p1	ycf1	1	GCAGCTGTGATGGAACCTTA	ACATTGTCTCTTCGCGTCTC	20	20	50.0	50.0	57.9	58.0	16.6	5.8	0.129888	101944	102093	482-698	1.000000	86.4
ycf1_p2	ycf1	2	GCAGCTGTGATGGAACCTTA	CATTGTCTCTTCGCGTCTCA	20	20	50.0	50.0	57.9	58.0	16.6	5.8	0.131248	101944	102093	481-697	1.000000	86.4
ycf1_p3	ycf1	3	ACCGGTACAACATATCCCCA	ACATTGTCTCTTCGCGTCTC	20	20	50.0	50.0	58.1	58.0	4.5	5.8	0.133719	101944	102093	613-829	1.000000	85.8
ycf1_p4	ycf1	4	ACCGGTACAACATATCCCCA	CATTGTCTCTTCGCGTCTCA	20	20	50.0	50.0	58.1	58.0	4.5	5.8	0.135079	101944	102093	612-828	1.000000	85.8
ycf1_p5	ycf1	5	GCAGCTGTGATGGAACCTTA	TTACCGGCTTTATCGTTCCG	20	20	50.0	50.0	57.9	58.1	16.6	0.0	0.188631	101944	102093	752-968	1.000000	85.5
psaC-ccsA_p1	psaC-ccsA	1	TTGTACCCAATGCGTACGAG	GGAGCAATATGGGCCAATGA	20	20	50.0	50.0	58.0	58.0	21.7	11.9	0.001304	105845	107780	2147-2394	1.000000	85.3
psaC-ccsA_p2	psaC-ccsA	2	TTGTACCCAATGCGTACGAG	CTGGAGCAATATGGGCCAAT	20	20	50.0	50.0	58.0	58.0	21.7	11.9	0.006322	105845	107780	2149-2396	1.000000	85.3
psaC-ccsA_p3	psaC-ccsA	3	TTGTACCCAATGCGTACGAG	GGATCCGTTATTTGGGGGTC	20	20	50.0	55.0	58.0	58.0	21.7	0.0	0.023896	105845	107780	1975-2222	1.000000	85.3
psaC-ccsA_p4	psaC-ccsA	4	ATTCGGGGTGATCTAGAGGG	GGAGCAATATGGGCCAATGA	20	20	55.0	50.0	58.0	58.0	0.0	11.9	0.017257	105845	107780	2410-2654	0.916667	81.8
psaC-ccsA_p5	psaC-ccsA	5	ATTCGGGGTGATCTAGAGGG	CTGGAGCAATATGGGCCAAT	20	20	55.0	50.0	58.0	58.0	0.0	11.9	0.022275	105845	107780	2412-2656	0.916667	81.8
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	TGGAAGGGCTCACATTAGGT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.3	57.7	0.0	16.5	0.588865	111698	113248	1506-1700	1.000000	80.5
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	111698	113248	1448-1642	1.000000	80.4
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.4	57.7	0.0	16.5	0.611502	111698	113248	1473-1667	1.000000	80.3
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	GTTTGATGGAAGGGCTCACA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.1	57.7	0.0	16.5	0.347991	111698	113248	1512-1704	0.916667	78.9
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	TGTTTGATGGAAGGGCTCAC	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.1	57.7	0.0	16.5	0.347991	111698	113248	1513-1705	0.916667	78.9
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	CAGAGGGAATGGCAATGCTA	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.6	58.0	4.8	5.8	0.361187	114565	116151	1669-1704	1.000000	81.7
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	CAGAGGGAATGGCAATGCTA	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.6	58.0	4.8	5.8	0.362557	114565	116151	1667-1702	1.000000	81.7
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	TTAATGGATGCTGCCAGAGG	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.6	58.0	0.0	5.8	0.361187	114565	116151	1683-1718	1.000000	81.6
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	TTAATGGATGCTGCCAGAGG	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	5.8	0.362557	114565	116151	1681-1716	1.000000	81.6
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	CAGAGGGAATGGCAATGCTA	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.6	58.2	4.8	0.0	0.550023	114565	116151	1664-1699	1.000000	80.2