primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	20	22	55.0	45.5	58.1	58.2	0.0	0.0	2.331881	132	571	480-512	0.866667	67.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCATCTACAAATGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.336243	132	571	494-526	0.866667	67.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCATCTACAAATGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.337750	132	571	493-525	0.866667	67.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TCGAAGCTCCATCTACAAATGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.337750	132	571	495-527	0.866667	67.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	20	21	55.0	47.6	58.1	56.8	0.0	0.0	2.358974	132	571	479-511	0.866667	67.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.087178	13740	14970	1346-1387	1.000000	83.0
atpH-atpI_p2	atpH-atpI	2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.186809	13740	14970	1429-1470	1.000000	81.9
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.190326	13740	14970	1433-1474	1.000000	81.9
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	20	20	45.0	50.0	58.2	58.0	1.4	0.0	0.232716	13740	14970	1347-1388	1.000000	81.8
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.263178	13740	14970	1361-1402	1.000000	81.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.0	58.1	0.0	0.0	1.094688	27232	28553	1346-1407	1.000000	75.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.163184	27232	28553	1351-1412	1.000000	75.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.2	58.1	0.0	0.0	1.236113	27232	28553	1345-1406	1.000000	74.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CTCATTTCCATCCCCGAGC	AGTCCTCTGCCTTACCACTC	19	20	57.9	55.0	58.0	58.4	0.0	0.0	1.449777	27232	28553	1315-1376	1.000000	73.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	19	20	52.6	50.0	57.6	58.1	0.0	0.0	1.509031	27232	28553	1344-1405	1.000000	72.6
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	CAATGCCGGCAACAATGAAA	TTTGAACTGGTGACACGAGG	20	20	45.0	50.0	58.2	58.1	36.3	20.1	0.258638	49681	50317	692-842	1.000000	88.9
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	CAATGCCGGCAACAATGAAA	AATCATGTGCCAGGAACCAG	20	20	45.0	50.0	58.2	58.2	36.3	0.0	0.363374	49681	50317	713-863	1.000000	87.9
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	49681	50317	639-789	1.000000	87.2
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	CAATGCCGGCAACAATGAAA	GTGCCAGGAACCAGATTTGA	20	20	45.0	50.0	58.2	58.1	36.3	0.0	0.297042	49681	50317	707-857	0.933333	85.2
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	CAATGCCGGCAACAATGAAA	CATGTGCCAGGAACCAGATT	20	20	45.0	50.0	58.2	58.2	36.3	0.0	0.363374	49681	50317	710-860	0.933333	84.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTCCGGAAATGAAAAATGCCA	AGAAGGTCTACGGTTCGAGT	21	20	42.9	50.0	57.4	57.8	5.2	0.0	1.788479	52731	53865	1206-1267	1.000000	70.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTCCGGAAATGAAAAATGCC	AGAAGGTCTACGGTTCGAGT	21	20	42.9	50.0	57.4	57.8	5.2	0.0	1.790181	52731	53865	1207-1268	1.000000	70.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCGGAAATGAAAAATGCCA	AGAAGGTCTACGGTTCGAGT	20	20	40.0	50.0	56.2	57.8	0.0	0.0	1.998559	52731	53865	1205-1266	1.000000	68.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTCCGGAAATGAAAAATGCCA	GAAGGTCTACGGTTCGAGTC	21	20	42.9	55.0	57.4	57.5	5.2	0.0	2.132251	52731	53865	1205-1266	1.000000	67.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACTCCGGAAATGAAAAATGCC	GAAGGTCTACGGTTCGAGTC	21	20	42.9	55.0	57.4	57.5	5.2	0.0	2.133953	52731	53865	1206-1267	1.000000	67.4
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	5.4	0.0	0.491221	65710	66780	872-1153	1.000000	82.4
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	5.4	0.0	0.491221	65710	66780	874-1155	1.000000	82.4
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGGCCGAA	GGTTCATATTCCGGGTTGGG	20	20	45.0	55.0	57.7	58.3	5.4	0.0	0.598802	65710	66780	871-1152	1.000000	81.5
petA-psbJ_p4	petA-psbJ	4	GAGAAGGTTCAATTGGCCGA	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.2	57.6	5.4	0.0	0.607328	65710	66780	868-1149	1.000000	81.5
petA-psbJ_p5	petA-psbJ	5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGGTTGG	20	20	50.0	50.0	58.2	57.6	5.4	0.0	0.607328	65710	66780	873-1154	1.000000	81.5
clpP_p1	clpP	1	AGTTGGCATGTTGAATCGTA	ACGTTTCCAACTCAAAGTGA	20	20	40.0	40.0	55.4	55.4	0.0	0.0	5.184154	72909	74912	2166-2186	1.000000	41.2
clpP_p2	clpP	2	TAGTTGGCATGTTGAATCGT	ACGTTTCCAACTCAAAGTGA	20	20	40.0	40.0	55.4	55.4	0.0	0.0	5.184154	72909	74912	2167-2187	1.000000	41.2
clpP_p3	clpP	3	AGTTGGCATGTTGAATCGTA	ACGTTTCCAACTCAAAGTGAA	20	21	40.0	38.1	55.4	56.2	0.0	0.0	5.392157	72909	74912	2166-2186	1.000000	41.2
clpP_p4	clpP	4	TAGTTGGCATGTTGAATCGT	ACGTTTCCAACTCAAAGTGAA	20	21	40.0	38.1	55.4	56.2	0.0	0.0	5.392157	72909	74912	2167-2187	1.000000	41.2
clpP_p5	clpP	5	TGGGTTGGTTTAGATTGATCCT	ACGTTTCCAACTCAAAGTGA	22	20	40.9	40.0	57.1	55.4	0.0	0.0	5.498253	72909	74912	2134-2154	1.000000	41.2
petD-rpoA_p1	petD-rpoA	1	AGCGACGACTGTCTTTTTGA	TGAACATTTTCGTAGCGAAGA	20	21	45.0	38.1	57.8	55.9	6.2	4.2	3.269468	80716	80887	360-361	1.000000	57.4
petD-rpoA_p2	petD-rpoA	2	CAGTAGCGACGACTGTCTTT	TGAACATTTTCGTAGCGAAGA	20	21	50.0	38.1	57.7	55.9	6.2	4.2	3.383904	80716	80887	364-365	1.000000	56.4
petD-rpoA_p3	petD-rpoA	3	AGCGACGACTGTCTTTTTGA	TTGAACATTTTCGTAGCGAAGA	20	22	45.0	36.4	57.8	56.7	6.2	4.2	3.543768	80716	80887	361-362	1.000000	55.2
petD-rpoA_p4	petD-rpoA	4	TTTGATTGGTACCGCAGTCG	TGAACATTTTCGTAGCGAAGA	20	21	50.0	38.1	58.6	55.9	7.6	4.2	3.615960	80716	80887	345-346	1.000000	54.8
petD-rpoA_p5	petD-rpoA	5	CAGTAGCGACGACTGTCTTT	TTGAACATTTTCGTAGCGAAGA	20	22	50.0	36.4	57.7	56.7	6.2	4.2	3.658203	80716	80887	365-366	1.000000	54.2
infA-rps8_p1	infA-rps8	1	AGACCGTTGGTAAGCGATTC	GAGAGGCTCGACTAGAAGGA	20	20	50.0	55.0	58.0	57.7	0.0	9.2	0.341286	82929	83045	220-242	1.000000	80.5
infA-rps8_p2	infA-rps8	2	TAAACGAACCCGGAACAGAC	GAGAGGCTCGACTAGAAGGA	20	20	50.0	55.0	57.8	57.7	0.7	9.2	0.488682	82929	83045	236-258	1.000000	79.1
infA-rps8_p3	infA-rps8	3	AGACCGTTGGTAAGCGATTC	TCCGCGAATTTTAGGTGGAA	20	20	50.0	45.0	58.0	57.5	0.0	8.2	0.482097	82929	83045	285-307	1.000000	78.6
infA-rps8_p4	infA-rps8	4	AGACCGTTGGTAAGCGATTC	TTCCGCGAATTTTAGGTGGA	20	20	50.0	45.0	58.0	57.5	0.0	8.2	0.482097	82929	83045	286-308	1.000000	78.6
infA-rps8_p5	infA-rps8	5	CCGTTGGTAAGCGATTCAGT	GAGAGGCTCGACTAGAAGGA	20	20	50.0	55.0	58.3	57.7	0.0	9.2	0.613304	82929	83045	217-239	1.000000	78.4
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	59.2	58.1	29.3	0.0	1.260580	87050	87120	207-212	1.000000	74.1
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	87050	87120	274-279	1.000000	72.6
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	20	20	55.0	45.0	59.2	58.3	29.3	0.0	1.442713	87050	87120	209-214	1.000000	72.6
rps19-rpl2_p4	rps19-rpl2	4	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	20	20	55.0	50.0	59.2	57.8	29.3	0.0	1.379100	87050	87120	265-270	1.000000	72.3
rps19-rpl2_p5	rps19-rpl2	5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	56.6	58.1	18.5	0.0	1.483856	87050	87120	206-211	1.000000	72.3
rpl2_p1	rpl2	1	ACGTGTCACAAGCTTACTCC	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.416235	87121	88611	1638-1643	1.000000	78.4
rpl2_p2	rpl2	2	ACGTGTCACAAGCTTACTCC	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	57.8	58.3	0.0	6.3	0.486703	87121	88611	1645-1650	1.000000	77.9
rpl2_p3	rpl2	3	ACGTGTCACAAGCTTACTCC	ACGCTTCAACCGGGTTATTC	20	20	50.0	50.0	57.8	58.6	0.0	13.0	0.704527	87121	88611	1639-1644	1.000000	76.1
rpl2_p4	rpl2	4	CGTGTCACAAGCTTACTCCT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.6	58.3	0.0	13.0	0.706377	87121	88611	1637-1642	1.000000	76.1
rpl2_p5	rpl2	5	CGTGTCACAAGCTTACTCCT	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	57.6	58.3	0.0	6.3	0.776845	87121	88611	1644-1649	1.000000	75.5
ndhF-rpl32_p1	ndhF-rpl32	1	GAATAAGTCCCGCGCCTATT	TGCTACCCAATATCCCTTCCT	20	21	50.0	47.6	57.8	57.9	0.0	0.0	1.240176	114893	115678	942-952	1.000000	72.2
ndhF-rpl32_p2	ndhF-rpl32	2	CGGCGAAAACTTTTTGTTGC	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	0.0	0.0	1.420326	114893	115678	973-983	1.000000	70.7
ndhF-rpl32_p3	ndhF-rpl32	3	GCGAAAACTTTTTGTTGCCG	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	3.8	0.0	1.420326	114893	115678	971-981	1.000000	70.7
ndhF-rpl32_p4	ndhF-rpl32	4	GTCCCGCGCCTATTAACATA	TGCTACCCAATATCCCTTCCT	20	21	50.0	47.6	57.6	57.9	0.0	0.0	1.518745	114893	115678	936-946	1.000000	70.0
ndhF-rpl32_p5	ndhF-rpl32	5	GAATAAGTCCCGCGCCTATT	TGATGTCGAAGTGCGTTTTT	20	20	50.0	40.0	57.8	56.6	0.0	0.0	1.574935	114893	115678	891-901	1.000000	69.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	57.9	57.9	0.0	0.0	1.199135	115847	116822	1088-1120	1.000000	75.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.221916	115847	116822	1114-1146	1.000000	75.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	57.9	57.8	0.0	0.0	1.259861	115847	116822	1081-1113	1.000000	75.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	21	20	47.6	55.0	57.9	57.8	0.0	0.0	1.271152	115847	116822	1087-1119	1.000000	75.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	21	20	47.6	55.0	57.9	57.8	0.0	0.0	1.271152	115847	116822	1086-1118	1.000000	75.1
ycf1_p1	ycf1	1	TGCCCAATTTCCAGATTCAGT	TCTAAACTGTACCCCTCCCG	21	20	42.9	55.0	57.8	58.1	0.0	0.0	1.317325	129144	129263	453-501	0.866667	71.1
ycf1_p2	ycf1	2	TGCCCAATTTCCAGATTCAGT	TCCCGTGGAGAGCTAATGAA	21	20	42.9	50.0	57.8	58.1	0.0	0.0	1.313312	129144	129263	438-486	0.866667	71.0
ycf1_p3	ycf1	3	TGCCCAATTTCCAGATTCAGT	GAAGCCTGGGATAGCATTCC	21	20	42.9	55.0	57.8	58.1	0.0	0.0	1.271016	129144	129263	753-801	0.866667	69.4
ycf1_p4	ycf1	4	TGCCCAATTTCCAGATTCAGT	GGATAGCATTCCTTTTGCGC	21	20	42.9	50.0	57.8	57.9	0.0	0.0	1.299415	129144	129263	745-793	0.866667	69.3
ycf1_p5	ycf1	5	TGCCCAATTTCCAGATTCAGT	GCCTGGGATAGCATTCCTTT	21	20	42.9	50.0	57.8	57.6	0.0	0.0	1.545611	129144	129263	750-798	0.866667	67.3