primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	21	20	47.6	50.0	58.3	57.8	0.0	0.0	1.477007	3232	3926	861-865	1.000000	68.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTGAAAGAGAAGTGGG	CGTGCGGTGTAATTCCATTG	20	20	50.0	50.0	56.5	57.8	0.0	0.0	1.706995	3232	3926	861-865	1.000000	67.0
matK-trnK-UUU_p3	matK-trnK-UUU	3	CTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	20	20	50.0	50.0	56.5	57.8	0.0	0.0	1.706995	3232	3926	860-864	1.000000	67.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACTCCTGAAAGAGAAGTGGGT	TGGGTTGCTAACTCAATGGT	21	20	47.6	45.0	58.3	57.0	0.0	0.0	2.274236	3232	3926	802-806	1.000000	62.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTGAAAGAGAAGTGGGTA	CGTGCGGTGTAATTCCATTG	22	20	45.5	50.0	58.1	57.8	0.0	0.0	2.314458	3232	3926	861-865	1.000000	62.1
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	8476	9457	1127-1128	1.000000	78.5
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	8476	9457	1114-1115	1.000000	77.9
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	20	20	50.0	50.0	57.6	58.2	0.9	5.0	0.580969	8476	9457	1113-1114	1.000000	75.5
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	20	20	45.0	55.0	58.0	58.6	0.0	0.0	0.605692	8476	9457	1106-1107	1.000000	75.3
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.6	58.2	0.9	0.0	0.656018	8476	9457	1100-1101	1.000000	74.9
psbC_p1	psbC	1	TTCGTTTCCCAGGAAATCCG	TGGCTCGGCTAGATGGAATA	20	20	50.0	50.0	58.2	58.0	14.7	0.0	0.193607	10467	11888	1608-1608	1.000000	78.6
psbC_p2	psbC	2	GCAGCGGAAGATCCTGAATT	TGGCTCGGCTAGATGGAATA	20	20	50.0	50.0	58.3	58.0	0.6	0.0	0.336513	10467	11888	1587-1587	1.000000	77.5
psbC_p3	psbC	3	TTCGTTTCCCAGGAAATCCG	AACCGAGCCAGAACAGAAAA	20	20	50.0	45.0	58.2	57.7	14.7	0.0	0.525772	10467	11888	1589-1589	1.000000	75.9
psbC_p4	psbC	4	TTCGTTTCCCAGGAAATCCG	ACCGAGCCAGAACAGAAAAA	20	20	50.0	45.0	58.2	57.7	14.7	0.0	0.525772	10467	11888	1588-1588	1.000000	75.9
psbC_p5	psbC	5	TTCGTTTCCCAGGAAATCCG	GGAATAACCGAGCCAGAACA	20	20	50.0	50.0	58.2	57.6	14.7	0.0	0.580515	10467	11888	1594-1594	1.000000	75.5
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	AGCCTTCCAAGCTAACGATG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234861	12664	13606	1097-1097	1.000000	78.2
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	TTGCTTCTCCTGATGGTTGG	CCTAGCCTTCCAAGCTAACG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.263697	12664	13606	1100-1100	1.000000	78.0
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	GCTTCTCCTGATGGTTGGTC	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.285842	12664	13606	1095-1095	1.000000	77.8
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GCTTCTCCTGATGGTTGGTC	CCTAGCCTTCCAAGCTAACG	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.314679	12664	13606	1098-1098	1.000000	77.6
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TTGCTTCTCCTGATGGTTGG	CCAACGTCGACTATAACCCC	20	20	50.0	55.0	57.8	57.8	0.0	14.7	0.419409	12664	13606	1118-1118	1.000000	76.7
psbM-petN_p1	psbM-petN	1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	20	20	40.0	50.0	54.5	58.2	0.0	0.0	3.670895	18665	19431	939-954	1.000000	52.6
psbM-petN_p2	psbM-petN	2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	20	20	40.0	60.0	54.5	58.3	0.0	0.0	3.801267	18665	19431	883-898	1.000000	51.7
psbM-petN_p3	psbM-petN	3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	20	20	40.0	55.0	54.5	58.4	0.0	0.0	3.891699	18665	19431	882-897	1.000000	50.9
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	38.1	50.0	55.2	58.2	0.0	0.0	3.908487	18665	19431	914-929	1.000000	50.7
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	21	20	38.1	60.0	55.2	58.3	0.0	0.0	4.038859	18665	19431	858-873	1.000000	49.8
rpoC1_p1	rpoC1	1	AGGGAGGAAGTTTGATCGGA	GCGCGGCAAAAATGATGATA	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.165607	24939	26987	2173-2173	1.000000	78.8
rpoC1_p2	rpoC1	2	AGGGAGGAAGTTTGATCGGA	ACTAGCGAAATCGGAATGCC	20	20	50.0	50.0	58.0	58.4	0.0	4.8	0.464325	24939	26987	2154-2154	1.000000	76.4
rpoC1_p3	rpoC1	3	AGGGAGGAAGTTTGATCGGA	GGCATCGCCGAATCTAACTT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.464325	24939	26987	2120-2120	1.000000	76.4
rpoC1_p4	rpoC1	4	AGGGAGGAAGTTTGATCGGA	ATCTCAATCTGGATTCGCGC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.540886	24939	26987	2189-2189	1.000000	75.8
rpoC1_p5	rpoC1	5	GGGAGGAAGTTTGATCGGAA	GCGCGGCAAAAATGATGATA	20	20	50.0	45.0	57.2	57.9	0.0	8.8	0.899909	24939	26987	2172-2172	1.000000	72.9
atpI-atpH_p1	atpI-atpH	1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	20	20	50.0	45.0	57.5	57.8	26.3	3.2	0.731910	33738	34552	884-884	1.000000	74.1
atpI-atpH_p2	atpI-atpH	2	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	58.6	57.8	16.5	3.2	0.790645	33738	34552	897-897	1.000000	73.7
atpI-atpH_p3	atpI-atpH	3	TAGCCGCAGCCTATATAGGT	AGCAGCAGCAATTAGTGGAT	20	20	50.0	45.0	57.5	57.6	26.3	3.2	0.958841	33738	34552	883-883	1.000000	72.3
atpI-atpH_p4	atpI-atpH	4	AGTCCCTATACCGGTCATGT	AAGCAGCAGCAATTAGTGGA	20	20	50.0	45.0	57.2	57.8	12.4	3.2	0.974624	33738	34552	952-952	1.000000	72.2
atpI-atpH_p5	atpI-atpH	5	ACAAGCGGTATTCAAGCTCT	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	57.2	57.8	0.0	3.2	0.975186	33738	34552	918-918	1.000000	72.2
atpA_p1	atpA	1	TTCCCCTTGCTTCCGAAAAA	TCTTTCTCAACGATTCCTCTTCT	20	23	45.0	39.1	57.9	57.3	0.0	0.0	3.805025	36738	38261	1610-1619	1.000000	49.6
atpA_p2	atpA	2	TTTCCCCTTGCTTCCGAAAA	TCTTTCTCAACGATTCCTCTTCT	20	23	45.0	39.1	57.9	57.3	0.0	0.0	3.805025	36738	38261	1611-1620	1.000000	49.6
atpA_p3	atpA	3	TTTTCCCCTTGCTTCCGAAA	TCTTTCTCAACGATTCCTCTTCT	20	23	45.0	39.1	57.9	57.3	0.0	0.0	3.805025	36738	38261	1612-1621	1.000000	49.6
atpA_p4	atpA	4	TTTTTCCCCTTGCTTCCGAA	TCTTTCTCAACGATTCCTCTTCT	20	23	45.0	39.1	57.9	57.3	0.0	0.0	3.805025	36738	38261	1613-1622	1.000000	49.6
atpA_p5	atpA	5	TTCCCCTTGCTTCCGAAAAA	TCTCAACGATTCCTCTTCTACT	20	22	45.0	40.9	57.9	56.1	0.0	0.0	4.024502	36738	38261	1606-1615	1.000000	47.9
psaA-ycf3_p1	psaA-ycf3	1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.480902	43786	44417	804-813	1.000000	77.0
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.499445	43786	44417	732-741	1.000000	77.0
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	43786	44417	800-809	1.000000	76.7
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	43786	44417	728-737	1.000000	76.7
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.7	58.0	0.0	18.7	0.667465	43786	44417	785-794	1.000000	75.6
ycf3_p1	ycf3	1	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGA	20	20	45.0	45.0	57.8	55.7	0.0	21.9	2.453412	44418	46417	2137-2145	1.000000	61.4
ycf3_p2	ycf3	2	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGA	20	20	45.0	45.0	57.8	55.7	0.0	21.9	2.453412	44418	46417	2136-2144	1.000000	61.4
ycf3_p3	ycf3	3	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGAT	20	21	45.0	42.9	57.8	56.3	0.0	29.8	2.890713	44418	46417	2137-2145	1.000000	57.9
ycf3_p4	ycf3	4	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGAT	20	21	45.0	42.9	57.8	56.3	0.0	29.8	2.890713	44418	46417	2136-2144	1.000000	57.9
ycf3_p5	ycf3	5	GATCAACAACCAAACCACCC	AGAGCATATGGATCGATCGA	20	20	50.0	45.0	57.2	55.7	0.0	21.9	3.098864	44418	46417	2138-2146	1.000000	56.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48365	49184	920-921	1.000000	80.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48365	49184	912-913	1.000000	79.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48365	49184	890-891	1.000000	79.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48365	49184	910-911	1.000000	78.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48365	49184	919-920	1.000000	77.9
ndhK_p1	ndhK	1	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	20	20	50.0	50.0	58.2	58.0	1.3	5.5	0.266827	51391	52131	979-979	1.000000	77.9
ndhK_p2	ndhK	2	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	20	20	45.0	50.0	57.8	58.0	1.3	5.5	0.271075	51391	52131	978-978	1.000000	77.9
ndhK_p3	ndhK	3	GATAACCAACCCTGCTGCAT	GGTTCAGTTTATGCATGGCG	20	20	50.0	50.0	58.2	57.8	1.3	10.2	0.425547	51391	52131	894-894	1.000000	76.7
ndhK_p4	ndhK	4	ATAACCAACCCTGCTGCATT	GGTTCAGTTTATGCATGGCG	20	20	45.0	50.0	57.8	57.8	1.3	10.2	0.429795	51391	52131	893-893	1.000000	76.7
ndhK_p5	ndhK	5	GATAACCAACCCTGCTGCAT	CGACGTATTGGGTGTATCCG	20	20	50.0	55.0	58.2	58.2	1.3	0.0	0.469822	51391	52131	955-955	1.000000	76.3
rbcL-psaI_p1	rbcL-psaI	1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.1	58.0	1.9	26.9	0.134794	58399	59687	1397-1434	1.000000	79.5
rbcL-psaI_p2	rbcL-psaI	2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.195661	58399	59687	1427-1464	1.000000	79.0
rbcL-psaI_p3	rbcL-psaI	3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.1	57.9	1.9	26.8	0.211328	58399	59687	1403-1440	1.000000	78.9
rbcL-psaI_p4	rbcL-psaI	4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.272195	58399	59687	1433-1470	1.000000	78.4
rbcL-psaI_p5	rbcL-psaI	5	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	13.1	26.9	0.274314	58399	59687	1398-1435	1.000000	78.4
petB_p1	petB	1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.6	58.2	0.0	0.0	1.620707	73822	75287	1569-1573	1.000000	67.3
petB_p2	petB	2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	20	20	50.0	50.0	57.6	56.7	0.0	0.0	1.668360	73822	75287	1569-1573	1.000000	66.9
petB_p3	petB	3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	20	21	50.0	47.6	57.6	57.7	0.0	0.0	1.712867	73822	75287	1575-1579	1.000000	66.6
petB_p4	petB	4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73822	75287	1574-1578	1.000000	65.1
petB_p5	petB	5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73822	75287	1575-1579	1.000000	65.1
rps15-ndhF_p1	rps15-ndhF	1	GTTTGCTGGCTTATTTGGCA	AAATATGTAGGGGGAGGGCG	20	20	45.0	55.0	57.5	58.9	0.0	0.0	1.389159	104687	105136	601-621	1.000000	71.0
rps15-ndhF_p2	rps15-ndhF	2	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATAT	20	19	45.0	57.9	57.5	57.9	0.0	0.0	1.538175	104687	105136	595-615	1.000000	69.8
rps15-ndhF_p3	rps15-ndhF	3	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATATC	20	20	45.0	60.0	57.5	59.1	0.0	0.0	1.550747	104687	105136	595-615	1.000000	69.7
rps15-ndhF_p4	rps15-ndhF	4	CGTTTGCTGGCTTATTTGGC	AAATATGTAGGGGGAGGGCG	20	20	50.0	55.0	58.7	58.9	0.0	0.0	1.585941	104687	105136	602-622	1.000000	69.4
rps15-ndhF_p5	rps15-ndhF	5	ACGTTTGCTGGCTTATTTGG	AAATATGTAGGGGGAGGGCG	20	20	45.0	55.0	57.3	58.9	0.0	0.0	1.658299	104687	105136	603-623	1.000000	68.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108457	109158	773-779	1.000000	78.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108457	109158	772-778	1.000000	78.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108457	109158	766-772	1.000000	78.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108457	109158	765-771	1.000000	78.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	58.1	58.2	0.0	4.7	0.313714	108457	109158	786-792	1.000000	78.2