primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	76	305	320-372	1.000000	89.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306133	76	305	334-386	1.000000	89.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	76	305	333-385	1.000000	89.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	76	305	335-387	1.000000	89.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.307703	76	305	322-374	1.000000	89.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.2	56.5	20.3	1.9	1.635593	4184	5097	970-1029	1.000000	78.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.2	56.5	20.3	1.9	1.635593	4184	5097	969-1028	1.000000	78.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.3	56.5	0.0	1.9	1.733010	4184	5097	984-1043	1.000000	77.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.3	56.5	5.3	1.9	1.749979	4184	5097	979-1038	1.000000	77.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AGGAACTTCCGCCTAATCAAA	20	21	50.0	42.9	58.2	57.3	20.3	1.9	1.882245	4184	5097	969-1028	1.000000	76.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6238	7230	817-1150	1.000000	90.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	TGACTGGCATAACATCCACG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.103051	6238	7230	1276-1603	1.000000	90.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGGATCGTGTCCTTCAAG	AGCAGCTTGCCAAACAAAAG	20	20	50.0	45.0	57.9	58.1	7.8	0.0	0.136254	6238	7230	1320-1647	1.000000	89.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AACGGATCGTGTCCTTCAAG	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	57.9	57.9	7.8	0.0	0.136380	6238	7230	1325-1652	1.000000	89.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AACGGATCGTGTCCTTCAAG	CGTGTGAATCGAGGGTTCAT	20	20	50.0	50.0	57.9	58.0	7.8	3.4	0.090431	6238	7230	1442-1770	0.957447	88.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8521	9077	622-637	1.000000	62.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8521	9077	621-636	1.000000	62.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8521	9077	625-640	1.000000	61.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8521	9077	624-639	1.000000	61.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8521	9077	623-638	1.000000	61.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.316428	26952	28047	1094-1209	1.000000	88.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CATTGCCTCATTTGCATCCC	TTTGGTAATGAGGCGACACC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.418225	26952	28047	1113-1228	1.000000	87.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.8	58.4	0.0	14.6	0.589791	26952	28047	1076-1191	1.000000	86.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.9	58.1	0.0	0.0	0.956495	26952	28047	1093-1208	1.000000	83.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ACATTGCCTCATTTGCATCC	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	57.0	58.1	0.0	0.0	1.055822	26952	28047	1095-1210	1.000000	82.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	31530	32776	1342-1420	1.000000	87.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	31530	32776	1345-1423	1.000000	87.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ATAGTGACTCGTCCGGGAAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.314904	31530	32776	1388-1466	0.978723	86.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	31530	32776	1341-1419	0.957447	84.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ATAGTGACTCGTCCGGGAAT	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.463380	31530	32776	1387-1465	0.936170	83.4
psaA-ycf3_p1	psaA-ycf3	1	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.5	57.8	0.0	0.0	0.648737	41635	42361	777-845	1.000000	83.0
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.676508	41635	42361	781-849	1.000000	82.8
psaA-ycf3_p3	psaA-ycf3	3	TTTTACTTCTGGTTCCGGCG	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.774905	41635	42361	776-844	1.000000	82.0
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	TCACGAGGCGTTTCGAATAA	20	20	50.0	45.0	58.5	57.6	0.0	15.7	0.835818	41635	42361	770-838	1.000000	81.6
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.5	57.7	0.0	19.9	0.760053	41635	42361	855-923	1.000000	81.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CGTTAGTCCTGGTAAAGCCC	GGATCAGGATCGCCCATTTT	20	20	55.0	50.0	58.0	58.0	0.0	3.4	0.033065	46071	46733	1273-1361	1.000000	90.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAGTCCTGGTAAAGCCCCAA	GGATCAGGATCGCCCATTTT	20	20	50.0	50.0	58.0	58.0	0.0	3.4	0.060887	46071	46733	1270-1358	1.000000	89.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TTAGTCCTGGTAAAGCCCCA	GGATCAGGATCGCCCATTTT	20	20	50.0	50.0	58.0	58.0	0.0	3.4	0.060887	46071	46733	1271-1359	1.000000	89.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CGTTAGTCCTGGTAAAGCCC	ACGCAGTCAACTCCATTTGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.052629	46071	46733	1370-1458	1.000000	89.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGTTAGTCCTGGTAAAGCCC	GGATTCCTCTACCAACGCAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.078623	46071	46733	1384-1472	0.978723	88.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.7	0.0	0.279423	50014	50967	1075-1106	1.000000	84.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.605515	50014	50967	1096-1127	1.000000	81.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.7	0.0	0.623195	50014	50967	1074-1105	1.000000	81.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	50014	50967	1063-1094	1.000000	80.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.6	57.8	0.0	0.0	0.766291	50014	50967	1088-1119	1.000000	80.6
ycf4-cemA_p1	ycf4-cemA	1	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	60191	60749	443-667	1.000000	89.8
ycf4-cemA_p2	ycf4-cemA	2	CGTTGACCCGTACTGATGAG	GAAATCCACCAGGGCAGAAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.167269	60191	60749	513-737	1.000000	89.5
ycf4-cemA_p3	ycf4-cemA	3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	20	20	45.0	50.0	58.2	58.1	33.5	0.0	0.298310	60191	60749	472-696	1.000000	88.6
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	20	20	45.0	50.0	57.7	58.1	8.4	0.0	0.400584	60191	60749	446-670	1.000000	88.1
ycf4-cemA_p5	ycf4-cemA	5	CGTTGACCCGTACTGATGAG	TCCACCAGGGCAGAAATACT	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.421798	60191	60749	509-733	1.000000	87.5
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62619	63647	1048-1141	1.000000	86.2
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62619	63647	1050-1143	1.000000	86.2
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62619	63647	1047-1140	1.000000	85.7
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62619	63647	1049-1142	1.000000	85.7
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	62619	63647	1047-1140	1.000000	85.4
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCGGGTGA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.064600	108752	110974	2519-2524	0.936170	83.2
ndhF_p2	ndhF	2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCGGGTGAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.064600	108752	110974	2518-2523	0.936170	83.2
ndhF_p3	ndhF	3	AAGAGGGATCCACCGAAGAA	AAAGAGGTAAGGTCGGGTGA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.067186	108752	110974	2511-2516	0.936170	83.2
ndhF_p4	ndhF	4	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCGGGTGAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.067186	108752	110974	2510-2515	0.936170	83.2
ndhF_p5	ndhF	5	GGGAGAAAGGGTGGAAAGTG	AAGAGGTAAGGTCGGGTGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.108750	108752	110974	2590-2595	0.936170	82.7
ndhF-rpl32_p1	ndhF-rpl32	1	CACGCATATTGATATATCTGTTCCA	TGATGCAGAAGTACGTTTTT	25	20	36.0	35.0	57.2	53.5	13.8	0.0	10.368618	110975	111479	479-580	0.978723	51.1
ndhF-rpl32_p2	ndhF-rpl32	2	CACGCATATTGATATATCTGTTCCA	TTGATGCAGAAGTACGTTTT	25	20	36.0	35.0	57.2	53.5	13.8	0.0	10.368618	110975	111479	480-581	0.978723	51.1
ndhF-rpl32_p3	ndhF-rpl32	3	CACGCATATTGATATATCTGTTCCA	TTTGATGCAGAAGTACGTTT	25	20	36.0	35.0	57.2	53.5	13.8	0.0	10.368618	110975	111479	481-582	0.978723	51.1
ndhF-rpl32_p4	ndhF-rpl32	4	CACGCATATTGATATATCTGTTCCA	TTTTGATGCAGAAGTACGTT	25	20	36.0	35.0	57.2	53.5	13.8	0.0	10.368618	110975	111479	482-583	0.978723	51.1
ndhF-rpl32_p5	ndhF-rpl32	5	CACGCATATTGATATATCTGTTCCA	TTTTTGATGCAGAAGTACGT	25	20	36.0	35.0	57.2	53.5	13.8	0.0	10.368618	110975	111479	483-584	0.978723	51.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGTGCGACAAACAAATAAACT	AGCGTGTCTACCGATTTCAC	21	20	33.3	50.0	54.9	58.0	0.0	0.0	4.096584	111657	112182	484-610	1.000000	59.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTGCGACAAACAAATAAACT	CAGCGTGTCTACCGATTTCA	21	20	33.3	50.0	54.9	58.0	0.0	0.0	4.097955	111657	112182	485-611	1.000000	59.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGTGCGACAAACAAATAAACT	TCCTAAGAGCAGCGTGTCTA	21	20	33.3	50.0	54.9	57.9	0.0	0.0	4.213031	111657	112182	494-620	1.000000	58.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTGCGACAAACAAATAAACT	CCTAAGAGCAGCGTGTCTAC	21	20	33.3	55.0	54.9	57.8	0.0	0.0	4.285049	111657	112182	493-619	1.000000	57.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTGCGACAAACAAATAAACT	CTAAGAGCAGCGTGTCTACC	21	20	33.3	55.0	54.9	57.8	0.0	0.0	4.285049	111657	112182	492-618	1.000000	57.7
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	112347	113318	1677-1686	1.000000	86.2
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033376	112347	113318	1676-1685	1.000000	86.2
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.037669	112347	113318	1676-1685	1.000000	86.2
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.039039	112347	113318	1675-1684	1.000000	86.2
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	TGACGAAATGGGCGGAATAG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	112347	113318	1727-1736	1.000000	85.9
rps15_p1	rps15	1	GATCAGCCGAAGAACACCAT	TACCAAGAGCAAGGACATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.143699	121079	121414	1193-1236	0.957447	85.7
rps15_p2	rps15	2	AACACCATGCATTGATGGGT	TACCAAGAGCAAGGACATGC	20	20	45.0	50.0	58.1	57.9	8.9	0.0	0.172483	121079	121414	1181-1224	0.936170	84.4
rps15_p3	rps15	3	CTTCTCCGCCAATAATGCCT	TACCAAGAGCAAGGACATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.140988	121079	121414	1743-1786	0.957447	84.3
rps15_p4	rps15	4	TCTTCTCCGCCAATAATGCC	TACCAAGAGCAAGGACATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.146542	121079	121414	1744-1787	0.957447	84.3
rps15_p5	rps15	5	CTCAAACGGTTTCACGCTTC	TACCAAGAGCAAGGACATGC	20	20	50.0	50.0	58.0	57.9	2.5	0.0	0.132577	121079	121414	1034-1048	0.382979	59.3
ycf1_p1	ycf1	1	TCGCAATTGAAAAAGCAAATAACG	AAGCATCGAAATCAAGAAGA	24	20	33.3	35.0	58.1	52.9	10.3	0.0	9.186715	122407	122526	186-186	0.978723	48.4
ycf1_p2	ycf1	2	TCGCAATTGAAAAAGCAAATAACG	AGCATCGAAATCAAGAAGAA	24	20	33.3	35.0	58.1	52.9	10.3	0.0	9.186715	122407	122526	185-185	0.957447	47.2
ycf1_p3	ycf1	3	CGCAATTGAAAAAGCAAATAACG	AGCATCGAAATCAAGAAGAA	23	20	34.8	35.0	56.8	52.9	10.3	0.0	9.266622	122407	122526	184-184	0.957447	47.2
ycf1_p4	ycf1	4	TCGCAATTGAAAAAGCAAATAACG	AGCATCAAATAAAAAGCATCGA	24	22	33.3	31.8	58.1	55.1	10.3	0.0	9.036394	122407	122526	199-199	0.957447	46.3
ycf1_p5	ycf1	5	CGCAATTGAAAAAGCAAATAACG	AGCATCAAATAAAAAGCATCGA	23	22	34.8	31.8	56.8	55.1	10.3	0.0	9.116301	122407	122526	198-198	0.957447	46.3